UGGT1, CPTAC-290 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
UGGT1 GT, UGCGL1, UGGT, UGT1, UGTR
Sequence Length (AA) Molecular Weight (Da)
1555 177190
Protein Name
UDP-glucose:glycoprotein glucosyltransferase 1
Sources
UniProt PhosphoSitePlus ® GeneCards Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCKGDASGA CAAGALPVTG VCYKMGVLVV LTVLWLFSSV KADSKAITTS
60 70 80 90 100
LTTKWFSTPL LLEASEFLAE DSQEKFWNFV EASQNIGSSD HDGTDYSYYH
110 120 130 140 150
AILEAAFQFL SPLQQNLFKF CLSLRSYSAT IQAFQQIAAD EPPPEGCNSF
160 170 180 190 200
FSVHGKKTCE SDTLEALLLT ASERPKPLLF KGDHRYPSSN PESPVVIFYS
210 220 230 240 250
EIGSEEFSNF HRQLISKSNA GKINYVFRHY IFNPRKEPVY LSGYGVELAI
260 270 280 290 300
KSTEYKAKDD TQVKGTEVNT TVIGENDPID EVQGFLFGKL RDLHPDLEGQ
310 320 330 340 350
LKELRKHLVE STNEMAPLKV WQLQDLSFQT AARILASPVE LALVVMKDLS
360 370 380 390 400
QNFPTKARAI TKTAVSSELR TEVEENQKYF KGTLGLQPGD SALFINGLHM
410 420 430 440 450
DLDTQDIFSL FDVLRNEARV MEGLHRLGIE GLSLHNVLKL NIQPSEADYA
460 470 480 490 500
VDIRSPAISW VNNLEVDSRY NSWPSSLQEL LRPTFPGVIR QIRKNLHNMV
510 520 530 540 550
FIVDPAHETT AELMNTAEMF LSNHIPLRIG FIFVVNDSED VDGMQDAGVA
560 570 580 590 600
VLRAYNYVAQ EVDDYHAFQT LTHIYNKVRT GEKVKVEHVV SVLEKKYPYV
610 620 630 640 650
EVNSILGIDS AYDRNRKEAR GYYEQTGVGP LPVVLFNGMP FEREQLDPDE
660 670 680 690 700
LETITMHKIL ETTTFFQRAV YLGELPHDQD VVEYIMNQPN VVPRINSRIL
710 720 730 740 750
TAERDYLDLT ASNNFFVDDY ARFTILDSQG KTAAVANSMN YLTKKGMSSK
760 770 780 790 800
EIYDDSFIRP VTFWIVGDFD SPSGRQLLYD AIKHQKSSNN VRISMINNPA
810 820 830 840 850
KEISYENTQI SRAIWAALQT QTSNAAKNFI TKMAKEGAAE ALAAGADIAE
860 870 880 890 900
FSVGGMDFSL FKEVFESSKM DFILSHAVYC RDVLKLKKGQ RAVISNGRII
910 920 930 940 950
GPLEDSELFN QDDFHLLENI ILKTSGQKIK SHIQQLRVEE DVASDLVMKV
960 970 980 990 1000
DALLSAQPKG DPRIEYQFFE DRHSAIKLRP KEGETYFDVV AVVDPVTREA
1010 1020 1030 1040 1050
QRLAPLLLVL AQLINMNLRV FMNCQSKLSD MPLKSFYRYV LEPEISFTSD
1060 1070 1080 1090 1100
NSFAKGPIAK FLDMPQSPLF TLNLNTPESW MVESVRTPYD LDNIYLEEVD
1110 1120 1130 1140 1150
SVVAAEYELE YLLLEGHCYD ITTGQPPRGL QFTLGTSANP VIVDTIVMAN
1160 1170 1180 1190 1200
LGYFQLKANP GAWILRLRKG RSEDIYRIYS HDGTDSPPDA DEVVIVLNNF
1210 1220 1230 1240 1250
KSKIIKVKVQ KKADMVNEDL LSDGTSENES GFWDSFKWGF TGQKTEEVKQ
1260 1270 1280 1290 1300
DKDDIINIFS VASGHLYERF LRIMMLSVLK NTKTPVKFWF LKNYLSPTFK
1310 1320 1330 1340 1350
EFIPYMANEY NFQYELVQYK WPRWLHQQTE KQRIIWGYKI LFLDVLFPLV
1360 1370 1380 1390 1400
VDKFLFVDAD QIVRTDLKEL RDFNLDGAPY GYTPFCDSRR EMDGYRFWKS
1410 1420 1430 1440 1450
GYWASHLAGR KYHISALYVV DLKKFRKIAA GDRLRGQYQG LSQDPNSLSN
1460 1470 1480 1490 1500
LDQDLPNNMI HQVPIKSLPQ EWLWCETWCD DASKKRAKTI DLCNNPMTKE
1510 1520 1530 1540 1550
PKLEAAVRIV PEWQDYDQEI KQLQIRFQKE KETGALYKEK TKEPSREGPQ
1555
KREEL

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-290 Collapse assay details

Data source: Panorama

Official Gene Symbol

UGGT1

Peptide Sequence

VWQLQDLSFQTAAR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

320

Peptide End

333

CPTAC ID

CPTAC-290

Peptide Molecular Mass

1,661.8526

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C,15N

LC

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y10 (1+) | 9.7 | 7 | 7.4 | 14.1 | 15.2 | 10 | 17.1 | 16.7 | 12.4 | 15 | 25 | 15 | | y9 (1+) | 15.6 | 11.5 | 8.6 | 25 | 20 | 11.6 | 29.5 | 23.1 | 14.4 | 15 | 25 | 15 | | y8 (1+) | 18.5 | 17.6 | 9.8 | 18.7 | 18.6 | 15.8 | 26.3 | 25.6 | 18.6 | 15 | 25 | 15 | | sum | 10 | 7.8 | 5.7 | 16 | 16.5 | 9.6 | 18.9 | 18.3 | 11.2 | 15 | 25 | 15 |

Additional Resources and Comments