CTTN, CPTAC-345 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MWKASAGHAV | SIAQDDAGAD | DWETDPDFVN | DVSEKEQRWG | AKTVQGSGHQ |
| 60 | 70 | 80 | 90 | 100 |
| EHINIHKLRE | NVFQEHQTLK | EKELETGPKA | SHGYGGKFGV | EQDRMDKSAV |
| 110 | 120 | 130 | 140 | 150 |
| GHEYQSKLSK | HCSQVDSVRG | FGGKFGVQMD | RVDQSAVGFE | YQGKTEKHAS |
| 160 | 170 | 180 | 190 | 200 |
| QKDYSSGFGG | KYGVQADRVD | KSAVGFDYQG | KTEKHESQRD | YSKGFGGKYG |
| 210 | 220 | 230 | 240 | 250 |
| IDKDKVDKSA | VGFEYQGKTE | KHESQKDYVK | GFGGKFGVQT | DRQDKCALGW |
| 260 | 270 | 280 | 290 | 300 |
| DHQEKLQLHE | SQKDYKTGFG | GKFGVQSERQ | DSAAVGFDYK | EKLAKHESQQ |
| 310 | 320 | 330 | 340 | 350 |
| DYSKGFGGKY | GVQKDRMDKN | ASTFEDVTQV | SSAYQKTVPV | EAVTSKTSNI |
| 360 | 370 | 380 | 390 | 400 |
| RANFENLAKE | KEQEDRRKAE | AERAQRMAKE | RQEQEEARRK | LEEQARAKTQ |
| 410 | 420 | 430 | 440 | 450 |
| TPPVSPAPQP | TEERLPSSPV | YEDAASFKAE | LSYRGPVSGT | EPEPVYSMEA |
| 460 | 470 | 480 | 490 | 500 |
| ADYREASSQQ | GLAYATEAVY | ESAEAPGHYP | AEDSTYDEYE | NDLGITAVAL |
| 510 | 520 | 530 | 540 | 550 |
| YDYQAAGDDE | ISFDPDDIIT | NIEMIDDGWW | RGVCKGRYGL | FPANYVELRQ |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-346 Collapse assay details
Data source: Panorama
Official Gene Symbol
CTTN
Peptide Sequence
TQTPPVSPAPQPTEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
399
Peptide End
414
CPTAC ID
CPTAC-346
Peptide Molecular Mass
1,733.8584
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 10 | 9.4 | 8.5 | 20.1 | 16.5 | 13 | 22.5 | 19 | 15.5 | 15 | 25 | 15 |
| y9 (1+) | 9.3 | 6.4 | 5.8 | 20 | 13.4 | 10.5 | 22.1 | 14.8 | 12 | 15 | 25 | 15 |
| y13 (2+) | 10.1 | 4.9 | 4.7 | 20.6 | 13.3 | 10.4 | 22.9 | 14.2 | 11.4 | 15 | 25 | 15 |
| sum | 9 | 5.1 | 4.9 | 20.3 | 13.4 | 10.5 | 22.2 | 14.3 | 11.6 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-345 Collapse assay details
Data source: Panorama
Official Gene Symbol
CTTN
Peptide Sequence
VDQSAVGFEYQGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
132
Peptide End
144
CPTAC ID
CPTAC-345
Peptide Molecular Mass
1,426.6729
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 7.6 | 7.9 | 4.3 | 9.2 | 9.4 | 4.3 | 11.9 | 12.3 | 6.1 | 15 | 25 | 15 |
| y8 (1+) | 6.5 | 6.3 | 4.4 | 6.4 | 7.3 | 4.6 | 9.1 | 9.6 | 6.4 | 15 | 25 | 15 |
| y7 (1+) | 3.9 | 7.7 | 4.4 | 4.5 | 7 | 4.6 | 6 | 10.4 | 6.4 | 15 | 25 | 15 |
| sum | 4 | 6.5 | 4 | 4 | 6.5 | 4 | 5.7 | 9.2 | 5.7 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-963 Collapse assay details
Data source: Panorama
Official Gene Symbol
CTTN
Peptide Modified Sequence
LPSS[+80.0]PVYEDAASFK
Modification Type
Phospho (ST)
Protein - Site of Modification
418
Peptide - Site of Modification
4
Peptide Start
415
Peptide End
428
CPTAC ID
CPTAC-963
Peptide Molecular Mass
1,589.7015
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (2+) | 66.5 | 41.3 | 48.7 | 78.7 | 40.9 | 56.5 | 103 | 58.1 | 74.6 | 15 | 24 | 15 |
| y13 (2+) | 32.8 | 9.9 | 8 | 19 | 9.8 | 13.1 | 37.9 | 13.9 | 15.3 | 15 | 24 | 15 |
| y8 (1+) | 24.5 | 11.3 | 9.2 | 34.2 | 11.8 | 14.1 | 42.1 | 16.3 | 16.8 | 15 | 24 | 15 |
| y10 (1+) | 33.9 | 9.8 | 9.1 | 28.7 | 9.3 | 13.7 | 44.4 | 13.5 | 16.4 | 15 | 24 | 15 |
| sum | 27.9 | 9.4 | 8.2 | 19.3 | 9.6 | 13.1 | 33.9 | 13.4 | 15.5 | 15 | 24 | 15 |