ERLIN2, CPTAC-361 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAQLGAVVAV	ASSFFCASLF	SAVHKIEEGH	IGVYYRGGAL	LTSTSGPGFH
60	70	80	90	100
LMLPFITSYK	SVQTTLQTDE	VKNVPCGTSG	GVMIYFDRIE	VVNFLVPNAV
110	120	130	140	150
YDIVKNYTAD	YDKALIFNKI	HHELNQFCSV	HTLQEVYIEL	FDQIDENLKL
160	170	180	190	200
ALQQDLTSMA	PGLVIQAVRV	TKPNIPEAIR	RNYELMESEK	TKLLIAAQKQ
210	220	230	240	250
KVVEKEAETE	RKKALIEAEK	VAQVAEITYG	QKVMEKETEK	KISEIEDAAF
260	270	280	290	300
LAREKAKADA	ECYTAMKIAE	ANKLKLTPEY	LQLMKYKAIA	SNSKIYFGKD
310	320	330	339
IPNMFMDSAG	SVSKQFEGLA	DKLSFGLEDE	PLETATKEN

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-362 Collapse assay details

Data source: Panorama

Official Gene Symbol

ERLIN2

Peptide Sequence

LSFGLEDEPLETATK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

323

Peptide End

337

CPTAC ID

CPTAC-362

Peptide Molecular Mass

1,648.8196

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Broad Institute and FHCRC

Submitting Lab PI

Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C,15N

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV equation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y10 (1+) | 16 | 10.6 | 17 | 40.6 | 19.2 | 21.1 | 43.6 | 21.9 | 27.1 | 15 | 25 | 15 | | y9 (1+) | 13.3 | 14.2 | 18.9 | 33.5 | 18.5 | 18.8 | 36 | 23.3 | 26.7 | 15 | 25 | 15 | | y8 (1+) | 20.2 | 33.6 | 24.7 | 34.7 | 37.8 | 24.3 | 40.2 | 50.6 | 34.6 | 15 | 25 | 15 | | sum | 9.3 | 10 | 12.7 | 34.3 | 15.3 | 18.4 | 35.5 | 18.3 | 22.4 | 15 | 25 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-361 Collapse assay details

Data source: Panorama

Official Gene Symbol

ERLIN2

Peptide Sequence

VAQVAEITYGQK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

221

Peptide End

232

CPTAC ID

CPTAC-361

Peptide Molecular Mass

1,305.6929

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Broad Institute and FHCRC

Submitting Lab PI

Steve Carr

Publication

View Details (opens in a new window)

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C,15N

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV equation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y9 (1+) | 16.9 | 10.4 | 5 | 16.2 | 10.7 | 6.7 | 23.4 | 14.9 | 8.4 | 15 | 25 | 15 | | y8 (1+) | 7 | 7.3 | 6.8 | 11 | 8.2 | 6.3 | 13 | 11 | 9.3 | 15 | 25 | 15 | | y7 (1+) | 8.3 | 10.6 | 9.7 | 11.1 | 11.5 | 11.1 | 13.9 | 15.6 | 14.7 | 15 | 25 | 15 | | sum | 6.4 | 6.4 | 3.1 | 8.2 | 7.3 | 4.9 | 10.4 | 9.7 | 5.8 | 15 | 25 | 15 |