CCT3, CPTAC-474 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-6084: View additional ELGIWEPLAVK data
CPTAC-475: View additional ELGIWEPLAVK data

10 20 30 40 50
MMGHRPVLVL SQNTKRESGR KVQSGNINAA KTIADIIRTC LGPKSMMKML
60 70 80 90 100
LDPMGGIVMT NDGNAILREI QVQHPAAKSM IEISRTQDEE VGDGTTSVII
110 120 130 140 150
LAGEMLSVAE HFLEQQMHPT VVISAYRKAL DDMISTLKKI SIPVDISDSD
160 170 180 190 200
MMLNIINSSI TTKAISRWSS LACNIALDAV KMVQFEENGR KEIDIKKYAR
210 220 230 240 250
VEKIPGGIIE DSCVLRGVMI NKDVTHPRMR RYIKNPRIVL LDSSLEYKKG
260 270 280 290 300
ESQTDIEITR EEDFTRILQM EEEYIQQLCE DIIQLKPDVV ITEKGISDLA
310 320 330 340 350
QHYLMRANIT AIRRVRKTDN NRIARACGAR IVSRPEELRE DDVGTGAGLL
360 370 380 390 400
EIKKIGDEYF TFITDCKDPK ACTILLRGAS KEILSEVERN LQDAMQVCRN
410 420 430 440 450
VLLDPQLVPG GGASEMAVAH ALTEKSKAMT GVEQWPYRAV AQALEVIPRT
460 470 480 490 500
LIQNCGASTI RLLTSLRAKH TQENCETWGV NGETGTLVDM KELGIWEPLA
510 520 530 540 545
VKLQTYKTAV ETAVLLLRID DIVSGHKKKG DDQSRQGGAP DAGQE

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6084 Collapse assay details

Data source: Panorama

Official Gene Symbol

CCT3

Peptide Sequence

ELGIWEPLAVK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

492

Peptide End

502

CPTAC ID

non-CPTAC-6084

Peptide Molecular Mass

1,253.7020

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

Bovine Serum Albumin

Submitting Laboratory

McGill University

Submitting Lab PI

Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6495B QQQ-MS

Internal Standard

Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

Infinity 1290

Column Packing

commercial (Agilent ZORBAX Eclipse Plus C18)

Column Dimensions

4.6 mm × 150 mm, (5 µm)

Flow Rate

0.4 mL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b3 (1+) | 8.9 | 5.1 | 8.1 | 8.9 | 5.6 | 8.5 | 12.6 | 7.6 | 11.7 | 16 | 16 | 17 | | y5 (1+) | 4.5 | 4.2 | 5.7 | 5.7 | 5.4 | 6.5 | 7.3 | 6.8 | 8.6 | 16 | 16 | 17 | | y6 (1+) | 11 | 4.2 | 4.9 | 9.1 | 5 | 4.9 | 14.3 | 6.5 | 6.9 | 16 | 16 | 17 | | y7 (1+) | 10.3 | 5.8 | 4.1 | 7.3 | 5.6 | 3.3 | 12.6 | 8.1 | 5.3 | 16 | 16 | 17 | | y9 (1+) | 5.6 | 5.2 | 4.6 | 4.6 | 5.3 | 4.5 | 7.2 | 7.4 | 6.4 | 16 | 16 | 17 | | sum | 4.9 | 2.2 | 2.3 | 2.6 | 2.3 | 1.3 | 5.5 | 3.2 | 2.6 | 16 | 16 | 17 |

Additional Resources and Comments

Assay Details for CPTAC-475 Collapse assay details

Data source: Panorama

Official Gene Symbol

CCT3

Peptide Sequence

ELGIWEPLAVK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

492

Peptide End

502

CPTAC ID

CPTAC-475

Peptide Molecular Mass

1,253.7020

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Seoul National University / Korea Institute of Science and Technology and FHCRC

Submitting Lab PI

Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y9 (1+) | 13.7 | 7.7 | 5.7 | 21.4 | 7.8 | 6.4 | 25.4 | 11 | 8.6 | 15 | 25 | 15 | | y7 (1+) | 7.3 | 7.4 | 5.8 | 17.5 | 8.1 | 6.4 | 19 | 11 | 8.6 | 15 | 25 | 15 | | y6 (1+) | 15.4 | 8.5 | 4.6 | 16.3 | 8.6 | 7.1 | 22.4 | 12.1 | 8.5 | 15 | 25 | 15 | | sum | 8.4 | 6.7 | 5.2 | 16.7 | 7.2 | 6.1 | 18.7 | 9.8 | 8 | 15 | 25 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-474 Collapse assay details

Data source: Panorama

Official Gene Symbol

CCT3

Peptide Modified Sequence

IPGGIIEDSC[+57.0]VLR

Modification Type

Carbamidomethyl Cysteine

Protein - Site of Modification

213

Peptide - Site of Modification

10

Peptide Start

204

Peptide End

216

CPTAC ID

CPTAC-474

Peptide Molecular Mass

1,427.7443

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Seoul National University / Korea Institute of Science and Technology and FHCRC

Submitting Lab PI

Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C,15N

LC

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y11 (1+) | 10.8 | 8.9 | 4.2 | 15.3 | 11.2 | 5.8 | 18.7 | 14.3 | 7.2 | 15 | 25 | 15 | | y8 (1+) | 9.1 | 9.5 | 4.9 | 13.3 | 10.3 | 7.5 | 16.1 | 14 | 9 | 15 | 25 | 15 | | y7 (1+) | 8.7 | 8.8 | 3.1 | 13 | 10.3 | 7.4 | 15.6 | 13.5 | 8 | 15 | 25 | 15 | | sum | 8.1 | 8.8 | 3.5 | 13.4 | 10.3 | 6.2 | 15.7 | 13.5 | 7.1 | 15 | 25 | 15 |

Additional Resources and Comments