DUT, CPTAC-64 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| DUT | N/A |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 252 | 26563 |
| Protein Name |
|---|
| Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MTPLCPRPAL | CYHFLTSLLR | SAMQNARGAR | QRAEAAVLSG | PGPPLGRAAQ |
| 60 | 70 | 80 | 90 | 100 |
| HGIPRPLSSA | GRLSQGCRGA | STVGAAGWKG | ELPKAGGSPA | PGPETPAISP |
| 110 | 120 | 130 | 140 | 150 |
| SKRARPAEVG | GMQLRFARLS | EHATAPTRGS | ARAAGYDLYS | AYDYTIPPME |
| 160 | 170 | 180 | 190 | 200 |
| KAVVKTDIQI | ALPSGCYGRV | APRSGLAAKH | FIDVGAGVID | EDYRGNVGVV |
| 210 | 220 | 230 | 240 | 250 |
| LFNFGKEKFE | VKKGDRIAQL | ICERIFYPEI | EEVQALDDTE | RGSGGFGSTG |
| 252 | ||||
| KN |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-65 Collapse assay details
Data source: Panorama
Official Gene Symbol
DUT
Peptide Sequence
GNVGVVLFNFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
195
Peptide End
206
CPTAC ID
CPTAC-65
Peptide Molecular Mass
1,249.6819
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 10.1 | 7.2 | 8.7 | 12.2 | 20.3 | 10.5 | 15.8 | 21.5 | 13.6 | 15 | 25 | 15 |
| y7 (1+) | 8.9 | 13.1 | 6.5 | 13.6 | 16.9 | 9 | 16.3 | 21.4 | 11.1 | 15 | 25 | 15 |
| y6 (1+) | 7.7 | 16.1 | 8.6 | 13.8 | 18.8 | 11.3 | 15.8 | 24.8 | 14.2 | 15 | 25 | 15 |
| sum | 5.6 | 8.5 | 7.1 | 11.9 | 14.6 | 9.5 | 13.2 | 16.9 | 11.9 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-64 Collapse assay details
Data source: Panorama
Official Gene Symbol
DUT
Peptide Modified Sequence
TDIQIALPSGC[+57.0]YGR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
166
Peptide - Site of Modification
11
Peptide Start
156
Peptide End
169
CPTAC ID
CPTAC-64
Peptide Molecular Mass
1,549.7559
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 49 | 11.6 | 11.4 | 54.6 | 13.5 | 15.7 | 73.4 | 17.8 | 19.4 | 15 | 25 | 15 |
| y8 (1+) | 46.7 | 15.1 | 8.6 | 53 | 13.4 | 10.4 | 70.6 | 20.2 | 13.5 | 15 | 25 | 15 |
| y7 (1+) | 31.1 | 8 | 5.2 | 34.3 | 8.3 | 9.6 | 46.3 | 11.5 | 10.9 | 15 | 25 | 15 |
| sum | 22.5 | 8.1 | 5.3 | 34.7 | 9.1 | 9 | 41.4 | 12.2 | 10.4 | 15 | 25 | 15 |