GRB2, CPTAC-699 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-1424: View additional ATADDELSFK data
CPTAC-1609: View additional ATADDELSFK data
CPTAC-1609: View additional ATADDELSFK data
CPTAC-1422: View additional FGNDVQHFK data
CPTAC-699: View additional FGNDVQHFK data
CPTAC-1423: View additional NYVTPVNR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MEAIAKYDFK | ATADDELSFK | RGDILKVLNE | ECDQNWYKAE | LNGKDGFIPK |
| 60 | 70 | 80 | 90 | 100 |
| NYIEMKPHPW | FFGKIPRAKA | EEMLSKQRHD | GAFLIRESES | APGDFSLSVK |
| 110 | 120 | 130 | 140 | 150 |
| FGNDVQHFKV | LRDGAGKYFL | WVVKFNSLNE | LVDYHRSTSV | SRNQQIFLRD |
| 160 | 170 | 180 | 190 | 200 |
| IEQVPQQPTY | VQALFDFDPQ | EDGELGFRRG | DFIHVMDNSD | PNWWKGACHG |
| 210 | 217 | |||
| QTGMFPRNYV | TPVNRNV |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1424 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
ATADDELSFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
11
Peptide End
20
CPTAC ID
CPTAC-1424
Peptide Molecular Mass
1,095.5084
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 2.5 | 1.8 | 1 | 3.2 | 6.3 | 3.8 | 4.1 | 6.6 | 3.9 | 15 | 15 | 15 |
| y6 (1+) | 5.3 | 2.8 | 2.9 | 7.1 | 7.2 | 3.3 | 8.9 | 7.7 | 4.4 | 15 | 15 | 15 |
| y7 (1+) | 3.5 | 2.7 | 2.3 | 6.4 | 6.2 | 3.8 | 7.3 | 6.8 | 4.4 | 15 | 15 | 15 |
| sum | 1.9 | 1.6 | 1.3 | 3.6 | 6.3 | 3.5 | 4.1 | 6.5 | 3.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1425 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
ESESAPGDFSLSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
87
Peptide End
100
CPTAC ID
CPTAC-1425
Peptide Molecular Mass
1,451.6780
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 17.4 | 6.3 | 2.3 | 21.2 | 10.4 | 5.1 | 27.4 | 12.2 | 5.6 | 15 | 15 | 15 |
| y9 (1+) | 3.8 | 1.3 | 1.8 | 7 | 6.3 | 2.8 | 8 | 6.4 | 3.3 | 15 | 15 | 15 |
| y10 (1+) | 15.9 | 4.8 | 4.5 | 19 | 7.2 | 5.2 | 24.8 | 8.7 | 6.9 | 15 | 15 | 15 |
| sum | 3.6 | 1.4 | 1.8 | 7.4 | 6.4 | 2.8 | 8.2 | 6.6 | 3.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1422 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
FGNDVQHFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
101
Peptide End
109
CPTAC ID
CPTAC-1422
Peptide Molecular Mass
1,090.5196
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 5.3 | 2.7 | 3.1 | 6.5 | 8.4 | 4.5 | 8.4 | 8.8 | 5.5 | 15 | 15 | 15 |
| y7 (2+) | 4.7 | 2.1 | 2.8 | 6.7 | 7.2 | 4.5 | 8.2 | 7.5 | 5.3 | 15 | 15 | 15 |
| y5 (2+) | 6.2 | 3.1 | 3 | 6.9 | 6.8 | 4.7 | 9.3 | 7.5 | 5.6 | 15 | 15 | 15 |
| sum | 4.3 | 2 | 2.3 | 4.6 | 7 | 4 | 6.3 | 7.3 | 4.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1421 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
FNSLNELVDYHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
125
Peptide End
136
CPTAC ID
CPTAC-1421
Peptide Molecular Mass
1,505.7263
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 3.8 | 2.9 | 2.7 | 10.2 | 5.7 | 4.7 | 10.9 | 6.4 | 5.4 | 15 | 15 | 15 |
| y10 (2+) | 6.2 | 3.8 | 3 | 12.7 | 6.9 | 4.5 | 14.1 | 7.9 | 5.4 | 15 | 15 | 15 |
| y4 (1+) | 3.1 | 1.9 | 2.3 | 10.6 | 6.3 | 3.8 | 11 | 6.6 | 4.4 | 15 | 15 | 15 |
| sum | 2.3 | 2.5 | 2.1 | 10.6 | 6.1 | 4 | 10.8 | 6.6 | 4.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1423 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
NYVTPVNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
208
Peptide End
215
CPTAC ID
CPTAC-1423
Peptide Molecular Mass
961.4981
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 2.4 | 2.5 | 1.5 | 3.1 | 6.1 | 2.9 | 3.9 | 6.6 | 3.3 | 15 | 15 | 15 |
| y6 (1+) | 2.8 | 2.6 | 1.2 | 3.7 | 6.2 | 2.7 | 4.6 | 6.7 | 3 | 15 | 15 | 15 |
| y5 (1+) | 2.8 | 2.5 | 1.2 | 3.8 | 6.3 | 2.7 | 4.7 | 6.8 | 3 | 15 | 15 | 15 |
| sum | 1.7 | 2.2 | 0.9 | 3.1 | 6.1 | 2.6 | 3.5 | 6.5 | 2.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-699 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
FGNDVQHFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
101
Peptide End
109
CPTAC ID
CPTAC-699
Peptide Molecular Mass
1,090.5196
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (2+) | 11.2 | 4.6 | 2 | 16.4 | 5.4 | 2.8 | 19.9 | 7.1 | 3.4 | 15 | 15 | 15 |
| y4 (1+) | 11.6 | 7 | 1.5 | 15.6 | 6.7 | 3.1 | 19.4 | 9.7 | 3.4 | 15 | 15 | 15 |
| y5 (1+) | 12.1 | 5 | 2.8 | 13.5 | 7.8 | 3.1 | 18.1 | 9.3 | 4.2 | 15 | 15 | 15 |
| sum | 8.2 | 3.7 | 1.2 | 11.9 | 5.6 | 2.6 | 14.5 | 6.7 | 2.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1609 Collapse assay details
Data source: Panorama
Official Gene Symbol
GRB2
Peptide Sequence
ATADDELSFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
11
Peptide End
20
CPTAC ID
CPTAC-1609
Peptide Molecular Mass
1,095.5084
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 16.1 | 7.2 | 4 | 19.2 | 8 | 6 | 25.1 | 10.8 | 7.2 | 15 | 15 | 15 |
| y7 (1+) | 13.7 | 5.3 | 2.9 | 18.1 | 7.8 | 6.4 | 22.7 | 9.4 | 7 | 15 | 15 | 15 |
| y8 (1+) | 7.4 | 2.8 | 1.4 | 13.6 | 6.4 | 5.3 | 15.5 | 7 | 5.5 | 15 | 15 | 15 |
| sum | 6.2 | 2.9 | 1.1 | 11.2 | 6.2 | 5 | 12.8 | 6.8 | 5.1 | 15 | 15 | 15 |