IL18, CPTAC-705 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-1431: View additional EDELGDR data
CPTAC-1435: View additional GMAVTISVK data
CPTAC-1432: View additional SDIIFFQR data
CPTAC-1250: View additional SDIIFFQR data
CPTAC-1433: View additional TIFIISMYK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAAEPVEDNC | INFVAMKFID | NTLYFIAEDD | ENLESDYFGK | LESKLSVIRN |
| 60 | 70 | 80 | 90 | 100 |
| LNDQVLFIDQ | GNRPLFEDMT | DSDCRDNAPR | TIFIISMYKD | SQPRGMAVTI |
| 110 | 120 | 130 | 140 | 150 |
| SVKCEKISTL | SCENKIISFK | EMNPPDNIKD | TKSDIIFFQR | SVPGHDNKMQ |
| 160 | 170 | 180 | 190 | 193 |
| FESSSYEGYF | LACEKERDLF | KLILKKEDEL | GDRSIMFTVQ | NED |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1431 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Sequence
EDELGDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
177
Peptide End
183
CPTAC ID
CPTAC-1431
Peptide Molecular Mass
832.3563
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.3 | 2.8 | 3.4 | 6.8 | 4.5 | 4.1 | 8 | 5.3 | 5.3 | 15 | 15 | 15 |
| y5 (1+) | 4.7 | 2.5 | 2.3 | 6.7 | 6.3 | 3.5 | 8.2 | 6.8 | 4.2 | 15 | 15 | 15 |
| y4 (1+) | 2.5 | 1.9 | 1.6 | 3.9 | 6.3 | 3.6 | 4.6 | 6.6 | 3.9 | 15 | 15 | 15 |
| sum | 2.2 | 1.8 | 1.7 | 4.3 | 6 | 3.4 | 4.8 | 6.3 | 3.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1435 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Sequence
GMAVTISVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
95
Peptide End
103
CPTAC ID
CPTAC-1435
Peptide Molecular Mass
904.5052
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 4.5 | 3.8 | 4.2 | 14.5 | 7.5 | 5.9 | 15.2 | 8.4 | 7.2 | 15 | 15 | 15 |
| y6 (1+) | 5.5 | 5.8 | 4 | 15.2 | 9.9 | 4.8 | 16.2 | 11.5 | 6.2 | 15 | 15 | 15 |
| y5 (1+) | 5.6 | 1.7 | 3.3 | 18.2 | 7 | 4 | 19 | 7.2 | 5.2 | 15 | 15 | 15 |
| sum | 3.5 | 2.9 | 3.4 | 15.6 | 7.6 | 4.4 | 16 | 8.1 | 5.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1434 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Modified Sequence
ISTLSC[+57.0]ENK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
112
Peptide - Site of Modification
6
Peptide Start
107
Peptide End
115
CPTAC ID
CPTAC-1434
Peptide Molecular Mass
1,050.5016
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 3.9 | 2.8 | 2.9 | 5.6 | 5.2 | 4 | 6.8 | 5.9 | 4.9 | 15 | 15 | 15 |
| y5 (1+) | 2.8 | 1.6 | 2.3 | 4.6 | 5.3 | 3.8 | 5.4 | 5.5 | 4.4 | 15 | 15 | 15 |
| y7 (1+) | 4.1 | 2.1 | 2.2 | 4.8 | 5.5 | 3.4 | 6.3 | 5.9 | 4 | 15 | 15 | 15 |
| sum | 2.6 | 1.4 | 2 | 4.2 | 5.2 | 3.4 | 4.9 | 5.4 | 3.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1432 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Sequence
SDIIFFQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
133
Peptide End
140
CPTAC ID
CPTAC-1432
Peptide Molecular Mass
1,024.5342
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 3 | 0.9 | 2.4 | 5.9 | 4.9 | 3.3 | 6.6 | 5 | 4.1 | 15 | 15 | 15 |
| y4 (1+) | 2.2 | 1 | 2.6 | 7.1 | 5.3 | 2.8 | 7.4 | 5.4 | 3.8 | 15 | 15 | 15 |
| y6 (1+) | 4.3 | 2.1 | 2 | 5.2 | 5.9 | 3.8 | 6.7 | 6.3 | 4.3 | 15 | 15 | 15 |
| sum | 2.4 | 0.8 | 2.4 | 6.5 | 5.2 | 2.8 | 6.9 | 5.3 | 3.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1433 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Sequence
TIFIISMYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
89
CPTAC ID
CPTAC-1433
Peptide Molecular Mass
1,114.6097
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.8 | 2.3 | 3.3 | 11.2 | 6.4 | 3.2 | 12.2 | 6.8 | 4.6 | 15 | 15 | 15 |
| y7 (1+) | 3.3 | 3.9 | 1.9 | 11.6 | 6.5 | 2.9 | 12.1 | 7.6 | 3.5 | 15 | 15 | 15 |
| y5 (1+) | 5.5 | 2.6 | 2.7 | 12.5 | 8 | 2.2 | 13.7 | 8.4 | 3.5 | 15 | 15 | 15 |
| sum | 3.3 | 2.4 | 1.5 | 11.5 | 6.6 | 1.7 | 12 | 7 | 2.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-705 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Modified Sequence
ISTLSC[+57.0]ENK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
112
Peptide - Site of Modification
6
Peptide Start
107
Peptide End
115
CPTAC ID
CPTAC-705
Peptide Molecular Mass
1,050.5016
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 22.8 | 4.2 | 2.1 | 27.2 | 7.3 | 3.1 | 35.5 | 8.4 | 3.7 | 15 | 15 | 15 |
| y7 (1+) | 14.7 | 4.5 | 1.9 | 26.4 | 6.4 | 3.4 | 30.2 | 7.8 | 3.9 | 15 | 15 | 15 |
| y8 (1+) | 17.7 | 6.3 | 2.2 | 23.8 | 7.1 | 3.7 | 29.7 | 9.5 | 4.3 | 15 | 15 | 15 |
| sum | 14.3 | 3.6 | 1.5 | 21.4 | 5.1 | 3.1 | 25.7 | 6.2 | 3.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5980 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Modified Sequence
ISTLSC[+57.021464]ENK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
107
Peptide End
115
CPTAC ID
CPTAC-5980
Peptide Molecular Mass
1,050.5016
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 6.9 | 3.4 | 1.9 | 8.6 | 12.1 | 13.1 | 11 | 12.6 | 13.2 | 13 | 25 | 14 |
| y7 (1+) | 5.9 | 2.7 | 1.8 | 9.2 | 10.8 | 13 | 10.9 | 11.1 | 13.1 | 13 | 25 | 14 |
| y5 (1+) | 6.9 | 2.8 | 2.8 | 8 | 10.6 | 13.4 | 10.6 | 11 | 13.7 | 13 | 25 | 14 |
| sum | 6.4 | 2.8 | 2.1 | 8.6 | 11 | 13.1 | 10.7 | 11.4 | 13.3 | 13 | 25 | 14 |
Additional Resources and Comments
Assay Details for CPTAC-1249 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Modified Sequence
ISTLSC[+57.0]ENK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
112
Peptide - Site of Modification
6
Peptide Start
107
Peptide End
115
CPTAC ID
CPTAC-1249
Peptide Molecular Mass
1,050.5016
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 11.7 | 8.7 | 6.9 | 13.5 | 12 | 8.6 | 17.9 | 14.8 | 11 | 15 | 15 | 15 |
| y6 (1+) | 12.5 | 10 | 6.8 | 17.3 | 10.8 | 9.1 | 21.3 | 14.7 | 11.4 | 15 | 15 | 15 |
| y8 (1+) | 10.3 | 6.9 | 7.4 | 16.2 | 10.4 | 7.3 | 19.2 | 12.5 | 10.4 | 15 | 15 | 15 |
| sum | 6 | 6.8 | 5.1 | 11.8 | 10.5 | 7.6 | 13.2 | 12.5 | 9.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1250 Collapse assay details
Data source: Panorama
Official Gene Symbol
IL18
Peptide Sequence
SDIIFFQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
133
Peptide End
140
CPTAC ID
CPTAC-1250
Peptide Molecular Mass
1,024.5342
Species
Homo sapiens (Human)
Assay Type
Direct ImmunoMRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 19.4 | 4.9 | 7.4 | 20.6 | 9.9 | 10.9 | 28.3 | 11 | 13.2 | 15 | 15 | 15 |
| y5 (1+) | 14.3 | 3.5 | 4.2 | 13.6 | 8.6 | 8.8 | 19.7 | 9.3 | 9.8 | 15 | 15 | 15 |
| y6 (1+) | 34.9 | 6.2 | 8.1 | 31.5 | 10.1 | 10.2 | 47 | 11.9 | 13 | 15 | 15 | 15 |
| sum | 13.6 | 3.5 | 5.7 | 15.6 | 8.7 | 9.4 | 20.7 | 9.4 | 11 | 15 | 15 | 15 |