CDK1, CPTAC-797 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MEDYTKIEKI | GEGTYGVVYK | GRHKTTGQVV | AMKKIRLESE | EEGVPSTAIR |
| 60 | 70 | 80 | 90 | 100 |
| EISLLKELRH | PNIVSLQDVL | MQDSRLYLIF | EFLSMDLKKY | LDSIPPGQYM |
| 110 | 120 | 130 | 140 | 150 |
| DSSLVKSYLY | QILQGIVFCH | SRRVLHRDLK | PQNLLIDDKG | TIKLADFGLA |
| 160 | 170 | 180 | 190 | 200 |
| RAFGIPIRVY | THEVVTLWYR | SPEVLLGSAR | YSTPVDIWSI | GTIFAELATK |
| 210 | 220 | 230 | 240 | 250 |
| KPLFHGDSEI | DQLFRIFRAL | GTPNNEVWPE | VESLQDYKNT | FPKWKPGSLA |
| 260 | 270 | 280 | 290 | 297 |
| SHVKNLDENG | LDLLSKMLIY | DPAKRISGKM | ALNHPYFNDL | DNQIKKM |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for non-CPTAC-6249 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
IGEGTY[+79.966331]GVVYK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
10
Peptide End
20
CPTAC ID
non-CPTAC-6249
Peptide Molecular Mass
1,264.5741
Species
Human
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
SKBR3 cells
Submitting Laboratory
Cell Signaling Technology
Submitting Lab PI
Yiying Zhu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Orbitrap Q Exactive (Thermo)
Internal Standard
Heavy isotope-labeled peptides
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
EASY-nLC II (Thermo)
Column Packing
self-packed
Column Dimensions
ID 100um, length 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 6.4 | 5.6 | 8 | 7.5 | 6.4 | 7 | 9.9 | 8.5 | 10.6 | 15 | 15 | 15 |
| y10 (1+) | 7.4 | 5.3 | 8.7 | 7.7 | 6.3 | 7.8 | 10.7 | 8.2 | 11.7 | 15 | 15 | 15 |
| y7 (1+) | 7.4 | 5.9 | 6 | 8 | 6.5 | 5.8 | 10.9 | 8.8 | 8.3 | 15 | 15 | 15 |
| y6 (1+) | 6.1 | 6.8 | 7.2 | 5.8 | 7 | 6 | 8.4 | 9.8 | 9.4 | 15 | 15 | 15 |
| y5 (1+) | 7.7 | 6.2 | 6.9 | 11.2 | 6 | 5.7 | 13.6 | 8.6 | 8.9 | 15 | 15 | 15 |
| y8 (2+) | 10.3 | 4.5 | 5.7 | 9.5 | 4.8 | 5 | 14 | 6.6 | 7.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5897 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Sequence
VYTHEVVTLWYR
Modification Type
unmodified
Protein - Site of Modification
159
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-5897
Peptide Molecular Mass
1,564.8038
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 24.9 | 11.3 | 7.9 | 24.6 | 21.2 | 7.5 | 35 | 24 | 10.9 | 15 | 27 | 15 |
| b7 (1+) | 25.3 | 13.3 | 5.9 | 31.5 | 24.9 | 9.1 | 40.4 | 28.2 | 10.8 | 15 | 27 | 15 |
| b6 (1+) | 13.9 | 15 | 9 | 30.7 | 21.9 | 9.1 | 33.7 | 26.5 | 12.8 | 15 | 27 | 15 |
| y10 (2+) | 31.5 | 14.7 | 9.8 | 37.3 | 25.8 | 6.5 | 48.8 | 29.7 | 11.8 | 15 | 27 | 15 |
| y11 (2+) | 20.5 | 13.4 | 12 | 24.6 | 22.1 | 8.5 | 32 | 25.8 | 14.7 | 15 | 27 | 15 |
| y3 (1+) | 18.2 | 9.3 | 8 | 17.6 | 18.7 | 8.4 | 25.3 | 20.9 | 11.6 | 15 | 27 | 15 |
| y5 (1+) | 20.7 | 12.5 | 8 | 20 | 18.4 | 8.7 | 28.8 | 22.2 | 11.8 | 15 | 27 | 15 |
| sum | 11.8 | 9.3 | 7.7 | 13.7 | 19.8 | 6.9 | 18.1 | 21.9 | 10.3 | 15 | 27 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5898 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
VYT[+79.966331]HEVVTLWYR
Modification Type
Phospho (ST)
Protein - Site of Modification
162
Peptide - Site of Modification
3
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-5898
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b7 (2+) | 15.3 | 8.7 | 5.4 | 24.1 | 19.6 | 5.7 | 28.5 | 21.4 | 7.9 | 15 | 27 | 15 |
| b7 (1+) | 18.8 | 9.4 | 5.1 | 19.2 | 19.2 | 7.1 | 26.9 | 21.4 | 8.7 | 15 | 27 | 15 |
| b6 (1+) | 17.9 | 8.6 | 4 | 17 | 21.2 | 6.3 | 24.7 | 22.9 | 7.5 | 15 | 27 | 15 |
| y3 (1+) | 10.9 | 6.6 | 4.5 | 14.1 | 19.4 | 10 | 17.8 | 20.5 | 11 | 15 | 27 | 15 |
| y4 (1+) | 16.8 | 9.9 | 5.2 | 18.9 | 19 | 8 | 25.3 | 21.4 | 9.5 | 15 | 27 | 15 |
| y5 (1+) | 14.4 | 6.9 | 4.1 | 17.3 | 17.1 | 9.4 | 22.5 | 18.4 | 10.3 | 15 | 27 | 15 |
| y6 (1+) | 23.9 | 8.9 | 4.4 | 23.9 | 14.8 | 7.7 | 33.8 | 17.3 | 8.9 | 15 | 27 | 15 |
| sum | 7.4 | 5.5 | 2.8 | 10.1 | 17.9 | 7 | 12.5 | 18.7 | 7.5 | 15 | 27 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1200 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Sequence
LESEEEGVPSTAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
37
Peptide End
50
CPTAC ID
CPTAC-1200
Peptide Molecular Mass
1,515.7417
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 18 | 6.7 | 4.4 | 22 | 7.1 | 5.6 | 28.4 | 9.8 | 7.1 | 15 | 15 | 15 |
| y8 (1+) | 27.4 | 8.4 | 3.3 | 30.3 | 13.3 | 3.6 | 40.9 | 15.7 | 4.9 | 15 | 15 | 15 |
| y9 (1+) | 35.3 | 12.9 | 6.6 | 48.6 | 13.3 | 9.5 | 60.1 | 18.5 | 11.6 | 15 | 15 | 15 |
| sum | 14.3 | 5.1 | 3.3 | 20.9 | 7.1 | 4.2 | 25.3 | 8.7 | 5.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-797 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
IGEGTY[+80.0]GVVYK
Modification Type
Phospho (Y)
Protein - Site of Modification
15
Peptide - Site of Modification
6
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-797
Peptide Molecular Mass
1,264.5741
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 14.8 | 8.2 | 10.4 | 16.1 | 12.1 | 10.2 | 21.9 | 14.6 | 14.6 | 15 | 15 | 15 |
| y5 (1+) | 15.7 | 15.6 | 7.5 | 18.9 | 15.3 | 7.6 | 24.6 | 21.9 | 10.7 | 15 | 15 | 15 |
| y6 (1+) | 15.2 | 12.9 | 15 | 15.9 | 12.6 | 13.3 | 22 | 18 | 20 | 15 | 15 | 15 |
| y7 (1+) | 32.8 | 18.3 | 9.5 | 31.7 | 18.7 | 14.7 | 45.6 | 26.2 | 17.5 | 15 | 15 | 15 |
| y8 (1+) | 16.1 | 6.7 | 6.6 | 11.4 | 13 | 7.4 | 19.7 | 14.6 | 9.9 | 15 | 15 | 15 |
| y10 (1+) | 19.3 | 10.4 | 5.8 | 21.1 | 10.5 | 7.2 | 28.6 | 14.8 | 9.2 | 15 | 15 | 15 |
| sum | 7.4 | 4.1 | 5.6 | 7.7 | 5.6 | 6.1 | 10.7 | 6.9 | 8.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-798 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Sequence
IGEGTYGVVYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-798
Peptide Molecular Mass
1,184.6077
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 11.7 | 4.8 | 3.8 | 10.4 | 4.4 | 3.6 | 15.7 | 6.5 | 5.2 | 15 | 15 | 15 |
| y4 (1+) | 18.1 | 11.4 | 10.2 | 23.6 | 13.5 | 12.4 | 29.7 | 17.7 | 16.1 | 15 | 15 | 15 |
| y5 (1+) | 10.7 | 4.2 | 5.7 | 10.6 | 4.6 | 5.3 | 15.1 | 6.2 | 7.8 | 15 | 15 | 15 |
| y6 (1+) | 10.5 | 9.9 | 8.7 | 11.4 | 12.8 | 9 | 15.5 | 16.2 | 12.5 | 15 | 15 | 15 |
| y7 (1+) | 21.6 | 11.6 | 19.3 | 24.7 | 11.9 | 14.9 | 32.8 | 16.6 | 24.4 | 15 | 15 | 15 |
| y8 (1+) | 11.9 | 5.2 | 8.2 | 12.9 | 6.4 | 8.3 | 17.6 | 8.2 | 11.7 | 15 | 15 | 15 |
| y9 (1+) | 11.7 | 8.7 | 9.4 | 12.6 | 10.5 | 9.4 | 17.2 | 13.6 | 13.3 | 15 | 15 | 15 |
| y10 (1+) | 9.6 | 5.4 | 3.3 | 12.2 | 4.9 | 3.2 | 15.5 | 7.3 | 4.6 | 15 | 15 | 15 |
| sum | 5.3 | 3.1 | 3.3 | 6.6 | 2.4 | 3.1 | 8.5 | 3.9 | 4.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1040 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
IGEGT[+80.0]YGVVYK
Modification Type
Phospho (ST)
Protein - Site of Modification
14
Peptide - Site of Modification
5
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-1040
Peptide Molecular Mass
1,264.5741
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 37.4 | 40.1 | 55.9 | 51.1 | 37.4 | 83 | 63.3 | 54.8 | 100.1 | 15 | 15 | 15 |
| y5 (1+) | 19.1 | 9.8 | 6.8 | 20.7 | 11.1 | 7.8 | 28.2 | 14.8 | 10.3 | 15 | 15 | 15 |
| y4 (1+) | 25.1 | 15.9 | 7.5 | 27.2 | 16 | 15.9 | 37 | 22.6 | 17.6 | 15 | 15 | 15 |
| sum | 14.3 | 8.8 | 4.8 | 14.8 | 9.3 | 7.1 | 20.6 | 12.8 | 8.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3054 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Sequence
LESEEEGVPSTAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
37
Peptide End
50
CPTAC ID
CPTAC-3054
Peptide Molecular Mass
1,515.7417
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.9 | 2.4 | 1.2 | 4.6 | 2.6 | 1.9 | 6.7 | 3.5 | 2.2 | 15 | 15 | 15 |
| y8 (1+) | 3.7 | 2.6 | 2.2 | 4.3 | 2.7 | 2.3 | 5.7 | 3.7 | 3.2 | 15 | 15 | 15 |
| y9 (1+) | 6 | 2.5 | 2.3 | 6.3 | 3.1 | 2.9 | 8.7 | 4 | 3.7 | 15 | 15 | 15 |
| sum | 4.6 | 2.4 | 1.3 | 4.5 | 2.6 | 1.9 | 6.4 | 3.5 | 2.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-801 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Sequence
VYTHEVVTLWYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-801
Peptide Molecular Mass
1,564.8038
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 12.3 | 4.4 | 4 | 10.3 | 4.7 | 4 | 16 | 6.4 | 5.7 | 15 | 15 | 15 |
| y6 (1+) | 10.5 | 12.1 | 11.1 | 9.2 | 12.8 | 11.5 | 14 | 17.6 | 16 | 15 | 15 | 15 |
| y7 (1+) | 11.6 | 5.9 | 9.2 | 16.3 | 6.9 | 10.9 | 20 | 9.1 | 14.3 | 15 | 15 | 15 |
| y8 (1+) | 9.4 | 6.5 | 11.4 | 10.5 | 9.6 | 11.3 | 14.1 | 11.6 | 16.1 | 15 | 15 | 15 |
| b6 (1+) | 8.4 | 9 | 8.6 | 15.6 | 9.6 | 9.8 | 17.7 | 13.2 | 13 | 15 | 15 | 15 |
| b5 (1+) | 12.4 | 4.8 | 5.7 | 12.4 | 4.9 | 6.1 | 17.5 | 6.9 | 8.3 | 15 | 15 | 15 |
| sum | 5.7 | 3.3 | 2.2 | 5 | 3 | 2.1 | 7.6 | 4.5 | 3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1041 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
VY[+80.0]THEVVTLWYR
Modification Type
Phospho (Y)
Protein - Site of Modification
160
Peptide - Site of Modification
2
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-1041
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 44.3 | 9.7 | 12.6 | 56.2 | 5.7 | 11.2 | 71.6 | 11.3 | 16.9 | 15 | 15 | 15 |
| y4 (1+) | 34.5 | 16.6 | 16.6 | 41.1 | 15.3 | 12.5 | 53.7 | 22.6 | 20.8 | 15 | 15 | 15 |
| y3 (1+) | 19 | 14.5 | 14.8 | 32.3 | 13.5 | 14.8 | 37.5 | 19.8 | 20.9 | 15 | 15 | 15 |
| sum | 23.1 | 8.6 | 7.1 | 28.7 | 7.4 | 7.2 | 36.8 | 11.3 | 10.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1042 Collapse assay details
Data source: Panorama
Official Gene Symbol
CDK1
Peptide Modified Sequence
VYT[+80.0]HEVVTLWYR
Modification Type
Phospho (ST)
Protein - Site of Modification
161
Peptide - Site of Modification
3
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-1042
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 39.6 | 11.5 | 13.3 | 45.8 | 14 | 18.3 | 60.5 | 18.1 | 22.6 | 15 | 15 | 15 |
| y4 (1+) | 36.1 | 33.7 | 12.7 | 55.1 | 33.9 | 12.2 | 65.9 | 47.8 | 17.6 | 15 | 15 | 15 |
| y3 (1+) | 48 | 17.5 | 14.3 | 45.8 | 20.1 | 16.2 | 66.3 | 26.7 | 21.6 | 15 | 15 | 15 |
| sum | 18.2 | 12.2 | 7.8 | 27.2 | 12.6 | 11.7 | 32.7 | 17.5 | 14.1 | 15 | 15 | 15 |