GSK3B, CPTAC-804 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-5749: View additional LLEYTPTAR data
non-CPTAC-5556: View additional LLEYTPTAR data
non-CPTAC-5556: View additional LLEYTPTAR data
non-CPTAC-5403: View additional QTLPVIYVK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MSGRPRTTSF | AESCKPVQQP | SAFGSMKVSR | DKDGSKVTTV | VATPGQGPDR |
| 60 | 70 | 80 | 90 | 100 |
| PQEVSYTDTK | VIGNGSFGVV | YQAKLCDSGE | LVAIKKVLQD | KRFKNRELQI |
| 110 | 120 | 130 | 140 | 150 |
| MRKLDHCNIV | RLRYFFYSSG | EKKDEVYLNL | VLDYVPETVY | RVARHYSRAK |
| 160 | 170 | 180 | 190 | 200 |
| QTLPVIYVKL | YMYQLFRSLA | YIHSFGICHR | DIKPQNLLLD | PDTAVLKLCD |
| 210 | 220 | 230 | 240 | 250 |
| FGSAKQLVRG | EPNVSYICSR | YYRAPELIFG | ATDYTSSIDV | WSAGCVLAEL |
| 260 | 270 | 280 | 290 | 300 |
| LLGQPIFPGD | SGVDQLVEII | KVLGTPTREQ | IREMNPNYTE | FKFPQIKAHP |
| 310 | 320 | 330 | 340 | 350 |
| WTKVFRPRTP | PEAIALCSRL | LEYTPTARLT | PLEACAHSFF | DELRDPNVKL |
| 360 | 370 | 380 | 390 | 400 |
| PNGRDTPALF | NFTTQELSSN | PPLATILIPP | HARIQAAAST | PTNATAASDA |
| 410 | 420 | |||
| NTGDRGQTNN | AASASASNST |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
loading
Assay Details for CPTAC-5749 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
LLEYTPTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
320
Peptide End
328
CPTAC ID
CPTAC-5749
Peptide Molecular Mass
1,062.5710
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 15.1 | 8.7 | 4.5 | 23.5 | 23 | 10.9 | 27.9 | 24.6 | 11.8 | 15 | 15 | 15 |
| y6 (1+) | 25.2 | 8.9 | 4.4 | 31.5 | 20.1 | 11.6 | 40.3 | 22 | 12.4 | 15 | 15 | 15 |
| y7 (1+) | 28.5 | 7.4 | 3.9 | 18.6 | 23.1 | 11 | 34 | 24.3 | 11.7 | 15 | 15 | 15 |
| sum | 18.8 | 7.3 | 3.9 | 21.1 | 22.1 | 11.1 | 28.3 | 23.3 | 11.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5750 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TPPEAIALC[+57.021464]SR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
309
Peptide End
319
CPTAC ID
CPTAC-5750
Peptide Molecular Mass
1,213.6125
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 21.3 | 12.3 | 5.6 | 23.1 | 27 | 15.2 | 31.4 | 29.7 | 16.2 | 15 | 15 | 15 |
| y5 (1+) | 43.1 | 12.8 | 6.4 | 44.4 | 26.8 | 12.7 | 61.9 | 29.7 | 14.2 | 15 | 15 | 15 |
| sum | 24 | 10.9 | 4.5 | 23.9 | 26.4 | 13.9 | 33.9 | 28.6 | 14.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5790 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTSFAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-5790
Peptide Molecular Mass
2,287.0613
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y15 (2+) | 12.1 | 4.2 | 9 | 16.7 | 16 | 12.4 | 20.6 | 16.5 | 15.3 | 16 | 15 | 17 |
| y8 (1+) | 14.1 | 4.1 | 9.2 | 19.5 | 15 | 12.4 | 24.1 | 15.6 | 15.4 | 16 | 15 | 17 |
| sum | 13 | 3.6 | 9.1 | 18.1 | 15.3 | 12.4 | 22.3 | 15.7 | 15.4 | 16 | 15 | 17 |
Additional Resources and Comments
Assay Details for CPTAC-5791 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+79.966331]FAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-5791
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 8.5 | 9.7 | 13.4 | 17.8 | 19.2 | 19.7 | 19.7 | 21.5 | 23.8 | 16 | 15 | 16 |
| y15 (2+) | 15.2 | 10.6 | 10.6 | 23.9 | 17.9 | 14.9 | 28.3 | 20.8 | 18.3 | 16 | 15 | 16 |
| sum | 9.1 | 9.9 | 11.7 | 19 | 18.6 | 16.8 | 21.1 | 21.1 | 20.5 | 16 | 15 | 16 |
Additional Resources and Comments
Assay Details for CPTAC-5751 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
VIGNGSFGVVYQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
61
Peptide End
74
CPTAC ID
CPTAC-5751
Peptide Molecular Mass
1,437.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 29.9 | 10.1 | 7.7 | 23.8 | 27.8 | 13.9 | 38.2 | 29.6 | 15.9 | 15 | 15 | 15 |
| y5 (1+) | 26.5 | 15.6 | 5.7 | 24.4 | 32.5 | 13.7 | 36 | 36.1 | 14.8 | 15 | 15 | 15 |
| y7 (1+) | 24.6 | 11.1 | 6.5 | 22.3 | 27.4 | 14.3 | 33.2 | 29.6 | 15.7 | 15 | 15 | 15 |
| sum | 24.2 | 11.5 | 6.5 | 20.5 | 29.1 | 13.8 | 31.7 | 31.3 | 15.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5556 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
LLEYTPTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
320
Peptide End
328
CPTAC ID
non-CPTAC-5556
Peptide Molecular Mass
1,062.5710
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 16.1 | 5.9 | 4 | 23.8 | 23.3 | 10.5 | 28.7 | 24 | 11.2 | 14 | 14 | 14 |
| y5 (1+) | 23.8 | 7.5 | 4.1 | 26.7 | 23.9 | 11.6 | 35.8 | 25 | 12.3 | 14 | 14 | 14 |
| y6 (1+) | 21.2 | 6.3 | 3.3 | 29.3 | 20.5 | 11 | 36.2 | 21.4 | 11.5 | 14 | 14 | 14 |
| y7 (1+) | 21.4 | 4.1 | 3.2 | 20.7 | 23.6 | 11 | 29.8 | 24 | 11.5 | 14 | 14 | 14 |
| sum | 18.2 | 4.1 | 3.2 | 22.8 | 22.7 | 10.9 | 29.2 | 23.1 | 11.4 | 14 | 14 | 14 |
Additional Resources and Comments
Assay Details for non-CPTAC-5403 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
QTLPVIYVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
151
Peptide End
159
CPTAC ID
non-CPTAC-5403
Peptide Molecular Mass
1,059.6328
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.2 | 2.8 | 2.6 | 6.1 | 3 | 3.7 | 7.4 | 4.1 | 4.5 | 15 | 15 | 15 |
| y7 (1+) | 9.4 | 6.3 | 3.8 | 6.3 | 7.2 | 4.1 | 11.3 | 9.6 | 5.6 | 15 | 15 | 15 |
| y3 (1+) | 9 | 4.1 | 3.4 | 11.5 | 4.5 | 3.2 | 14.6 | 6.1 | 4.7 | 15 | 15 | 15 |
| y5 (1+) | 18.1 | 6.8 | 5.3 | 20.9 | 6.4 | 4.2 | 27.6 | 9.3 | 6.8 | 15 | 15 | 15 |
| sum | 4.1 | 2 | 2.1 | 6.2 | 2.7 | 2.9 | 7.4 | 3.4 | 3.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5404 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TPPEAIALC[+57.021464]SR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
318
Peptide - Site of Modification
9
Peptide Start
309
Peptide End
319
CPTAC ID
non-CPTAC-5404
Peptide Molecular Mass
1,213.6125
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 2.4 | 1.7 | 1.8 | 3.3 | 2.1 | 1.9 | 4.1 | 2.7 | 2.6 | 15 | 15 | 15 |
| y5 (1+) | 6 | 2.5 | 1.4 | 5.5 | 3.5 | 3.4 | 8.1 | 4.3 | 3.7 | 15 | 15 | 15 |
| y6 (1+) | 9.1 | 2.4 | 2.3 | 9.9 | 2.9 | 4.6 | 13.4 | 3.8 | 5.1 | 15 | 15 | 15 |
| y7 (1+) | 5.7 | 2.2 | 2.7 | 6 | 2.5 | 4.4 | 8.3 | 3.3 | 5.2 | 15 | 15 | 15 |
| y8 (1+) | 8.2 | 3 | 3.5 | 7.5 | 4 | 3.3 | 11.1 | 5 | 4.8 | 15 | 15 | 15 |
| sum | 2.2 | 1.2 | 1.4 | 2.5 | 1.6 | 2.7 | 3.3 | 2 | 3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5401 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTSFAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
15
Peptide - Site of Modification
8
Peptide Start
7
Peptide End
27
CPTAC ID
non-CPTAC-5401
Peptide Molecular Mass
2,287.0613
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16 (2+) | 7.5 | 4.9 | 3.3 | 9.6 | 5.7 | 4.9 | 12.2 | 7.5 | 5.9 | 15 | 15 | 15 |
| y17 (2+) | 11 | 5.1 | 4.2 | 11.4 | 5.9 | 4.5 | 15.8 | 7.8 | 6.2 | 15 | 15 | 15 |
| y19 (2+) | 10.4 | 4.1 | 3 | 12.8 | 7.3 | 3.7 | 16.5 | 8.4 | 4.8 | 15 | 15 | 15 |
| y8 (1+) | 7 | 2.6 | 2.9 | 8.1 | 6.3 | 3.4 | 10.7 | 6.8 | 4.5 | 15 | 15 | 15 |
| y12 (1+) | 20 | 10.5 | 5.6 | 16.6 | 6.7 | 6.6 | 26 | 12.5 | 8.7 | 15 | 15 | 15 |
| sum | 3.3 | 2.1 | 1.8 | 5.1 | 3.8 | 2.9 | 6.1 | 4.3 | 3.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5705 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+79.966331]FAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
non-CPTAC-5705
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (2+) | 12.7 | 4.9 | 5.7 | 13 | 6.8 | 7.5 | 18.2 | 8.4 | 9.4 | 15 | 15 | 15 |
| y8 (1+) | 3.4 | 3.7 | 2.3 | 4.5 | 4 | 3.8 | 5.6 | 5.4 | 4.4 | 15 | 15 | 15 |
| y17 (2+) | 8.6 | 10.5 | 5.4 | 10.5 | 11.6 | 6.1 | 13.6 | 15.6 | 8.1 | 15 | 15 | 15 |
| y16 (2+) | 5.9 | 5.2 | 4.4 | 6.8 | 8.1 | 6.4 | 9 | 9.6 | 7.8 | 15 | 15 | 15 |
| y15 (2+) | 10.6 | 7.9 | 4.9 | 10.7 | 8.3 | 5.3 | 15.1 | 11.5 | 7.2 | 15 | 15 | 15 |
| sum | 3.6 | 2.8 | 1.8 | 4 | 3.2 | 2.4 | 5.4 | 4.3 | 3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5554 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
VIGNGSFGVVYQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
61
Peptide End
74
CPTAC ID
non-CPTAC-5554
Peptide Molecular Mass
1,437.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 28.4 | 9.3 | 7.3 | 22.9 | 28.5 | 13.2 | 36.5 | 30 | 15.1 | 14 | 14 | 14 |
| y5 (1+) | 29 | 12.7 | 5.9 | 25.8 | 33.4 | 13.5 | 38.8 | 35.7 | 14.7 | 14 | 14 | 14 |
| y7 (1+) | 26.5 | 9.6 | 5.1 | 23.5 | 28.8 | 12.6 | 35.4 | 30.4 | 13.6 | 14 | 14 | 14 |
| y8 (1+) | 29.1 | 10.8 | 3.8 | 30.6 | 33.8 | 13.9 | 42.2 | 35.5 | 14.4 | 14 | 14 | 14 |
| y12 (1+) | 23.7 | 8.2 | 6.9 | 30.9 | 27.3 | 14.2 | 38.9 | 28.5 | 15.8 | 14 | 14 | 14 |
| sum | 25.6 | 9.3 | 5.8 | 24.5 | 29.8 | 13.3 | 35.4 | 31.2 | 14.5 | 14 | 14 | 14 |
Additional Resources and Comments
Assay Details for CPTAC-3039 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Sequence
DIKPQNLLLDPDTAVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
181
Peptide End
197
CPTAC ID
CPTAC-3039
Peptide Molecular Mass
1,892.0619
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b6 (1+) | 5.3 | 8 | 7.1 | 7.5 | 13.7 | 12.2 | 9.2 | 15.9 | 14.1 | 15 | 15 | 15 |
| y7 (1+) | 5.2 | 7.5 | 7.2 | 7.7 | 12.8 | 12.3 | 9.3 | 14.8 | 14.3 | 15 | 15 | 15 |
| b7 (1+) | 5.4 | 8.2 | 7.4 | 7.5 | 14.1 | 12.2 | 9.2 | 16.3 | 14.3 | 15 | 15 | 15 |
| sum | 4.9 | 7.8 | 7.1 | 7.2 | 13.3 | 12.1 | 8.7 | 15.4 | 14 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3038 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
LTPLEAC[+57.0]AHSFFDELR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
336
Peptide - Site of Modification
7
Peptide Start
329
Peptide End
344
CPTAC ID
CPTAC-3038
Peptide Molecular Mass
1,904.9091
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 4 | 6.3 | 8.9 | 6.2 | 16.6 | 16.1 | 7.4 | 17.8 | 18.4 | 15 | 15 | 15 |
| y8 (1+) | 3.9 | 6.6 | 8.6 | 6 | 17.2 | 16.3 | 7.2 | 18.4 | 18.4 | 15 | 15 | 15 |
| y3 (1+) | 4.4 | 4.8 | 10.8 | 5.9 | 12.7 | 15.6 | 7.4 | 13.6 | 19 | 15 | 15 | 15 |
| sum | 3.3 | 5.9 | 9.2 | 5.4 | 15.9 | 15.9 | 6.3 | 17 | 18.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-804 Collapse assay details
Data source: Panorama
Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+80.0]FAESC[+57.0]KPVQQPSAFGSMK
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
9, 14
Peptide - Site of Modification
3, 8
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-804
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 25.6 | 12.5 | 8.5 | 33.1 | 17.8 | 10.8 | 41.8 | 21.8 | 13.7 | 15 | 15 | 15 |
| y4 (1+) | 28.8 | 15.5 | 9 | 19.8 | 15.7 | 13.3 | 34.9 | 22.1 | 16.1 | 15 | 15 | 15 |
| y5 (1+) | 21.1 | 11 | 10.5 | 18.8 | 9.3 | 10.6 | 28.3 | 14.4 | 14.9 | 15 | 15 | 15 |
| y6 (1+) | 23.2 | 12.1 | 6 | 21.6 | 15.9 | 8.9 | 31.7 | 20 | 10.7 | 15 | 15 | 15 |
| y7 (1+) | 24.9 | 7.7 | 18.6 | 26.9 | 8.4 | 15.7 | 36.7 | 11.4 | 24.3 | 15 | 15 | 15 |
| y8 (1+) | 8.9 | 6.6 | 2.9 | 9 | 7 | 5.6 | 12.7 | 9.6 | 6.3 | 15 | 15 | 15 |
| y12 (1+) | 9.9 | 9.1 | 9.2 | 13.6 | 8.1 | 10.9 | 16.8 | 12.2 | 14.3 | 15 | 15 | 15 |
| sum | 6.5 | 4.2 | 1.8 | 7 | 4.9 | 3.6 | 9.6 | 6.5 | 4 | 15 | 15 | 15 |