MAP2K1, CPTAC-808 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP2K1 | MEK1, PRKMK1 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 393 | 43439 |
| Protein Name |
|---|
| Dual specificity mitogen-activated protein kinase kinase 1 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
non-CPTAC-5411: View additional IPEQILGK data
CPTAC-1545: View additional LIHLEIKPAIR data
CPTAC-808: View additional QLMVHAFIK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MPKKKPTPIQ | LNPAPDGSAV | NGTSSAETNL | EALQKKLEEL | ELDEQQRKRL |
| 60 | 70 | 80 | 90 | 100 |
| EAFLTQKQKV | GELKDDDFEK | ISELGAGNGG | VVFKVSHKPS | GLVMARKLIH |
| 110 | 120 | 130 | 140 | 150 |
| LEIKPAIRNQ | IIRELQVLHE | CNSPYIVGFY | GAFYSDGEIS | ICMEHMDGGS |
| 160 | 170 | 180 | 190 | 200 |
| LDQVLKKAGR | IPEQILGKVS | IAVIKGLTYL | REKHKIMHRD | VKPSNILVNS |
| 210 | 220 | 230 | 240 | 250 |
| RGEIKLCDFG | VSGQLIDSMA | NSFVGTRSYM | SPERLQGTHY | SVQSDIWSMG |
| 260 | 270 | 280 | 290 | 300 |
| LSLVEMAVGR | YPIPPPDAKE | LELMFGCQVE | GDAAETPPRP | RTPGRPLSSY |
| 310 | 320 | 330 | 340 | 350 |
| GMDSRPPMAI | FELLDYIVNE | PPPKLPSGVF | SLEFQDFVNK | CLIKNPAERA |
| 360 | 370 | 380 | 390 | 393 |
| DLKQLMVHAF | IKRSDAEEVD | FAGWLCSTIG | LNQPSTPTHA | AGV |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-5745 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
71
Peptide End
84
CPTAC ID
CPTAC-5745
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 22.3 | 15.2 | 8.8 | 30.4 | 31.5 | 18.6 | 37.7 | 35 | 20.6 | 15 | 15 | 15 |
| y9 (1+) | 30.7 | 11.7 | 10.4 | 29.3 | 33.4 | 20.6 | 42.4 | 35.4 | 23.1 | 15 | 15 | 15 |
| y10 (1+) | 23.9 | 11.8 | 10.4 | 22.6 | 31.1 | 21.4 | 32.9 | 33.3 | 23.8 | 15 | 15 | 15 |
| sum | 23.3 | 12.6 | 9.8 | 25 | 31.5 | 20.4 | 34.2 | 33.9 | 22.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5411 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
IPEQILGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
161
Peptide End
168
CPTAC ID
non-CPTAC-5411
Peptide Molecular Mass
896.5331
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 11.6 | 7.6 | 2.4 | 14.6 | 7.7 | 3.1 | 18.6 | 10.8 | 3.9 | 15 | 15 | 15 |
| y6 (1+) | 15.9 | 3.5 | 1.4 | 17.9 | 3.5 | 3 | 23.9 | 4.9 | 3.3 | 15 | 15 | 15 |
| y7 (1+) | 7.4 | 2.8 | 1.1 | 9.5 | 3.7 | 1.9 | 12 | 4.6 | 2.2 | 15 | 15 | 15 |
| sum | 7.1 | 2.6 | 0.7 | 9.9 | 3.1 | 1.9 | 12.2 | 4 | 2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5557 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
71
Peptide End
84
CPTAC ID
non-CPTAC-5557
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 23.4 | 9.6 | 7 | 33.1 | 32 | 17.2 | 40.5 | 33.4 | 18.6 | 14 | 14 | 14 |
| y9 (1+) | 31.2 | 7.2 | 6.1 | 29.7 | 34.5 | 16.9 | 43.1 | 35.2 | 18 | 14 | 14 | 14 |
| y10 (1+) | 24.3 | 7.4 | 6.3 | 24.7 | 31.5 | 17.7 | 34.6 | 32.4 | 18.8 | 14 | 14 | 14 |
| y11 (1+) | 22.4 | 6.1 | 7.4 | 29.8 | 31.7 | 18.5 | 37.3 | 32.3 | 19.9 | 14 | 14 | 14 |
| sum | 23.2 | 7.2 | 6.4 | 26.9 | 32 | 17.5 | 35.5 | 32.8 | 18.6 | 14 | 14 | 14 |
Additional Resources and Comments
Assay Details for non-CPTAC-5704 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Modified Sequence
LC[+57.021464]DFGVSGQLIDS[+79.966331]MANSFVGTR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
206
Peptide End
227
CPTAC ID
non-CPTAC-5704
Peptide Molecular Mass
2,453.0756
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 6.3 | 3.6 | 3.7 | 5.2 | 4.1 | 5.1 | 8.2 | 5.5 | 6.3 | 15 | 15 | 15 |
| y4 (1+) | 11 | 7.3 | 4.3 | 4.4 | 8.8 | 6 | 11.8 | 11.4 | 7.4 | 15 | 15 | 15 |
| y8 (1+) | 14.1 | 8.9 | 7.2 | 13.4 | 7.9 | 7.1 | 19.5 | 11.9 | 10.1 | 15 | 15 | 15 |
| y7 (1+) | 13.7 | 6 | 5 | 13.2 | 4.6 | 6 | 19 | 7.6 | 7.8 | 15 | 15 | 15 |
| sum | 7.7 | 4.7 | 3.6 | 5.1 | 4.6 | 5 | 9.2 | 6.6 | 6.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5410 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
VSHKPSGLVMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
85
Peptide End
96
CPTAC ID
non-CPTAC-5410
Peptide Molecular Mass
1,280.7023
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 4.5 | 3.8 | 2.2 | 5.8 | 3.9 | 3.6 | 7.3 | 5.4 | 4.2 | 15 | 15 | 15 |
| y4 (1+) | 3.6 | 3.6 | 1.8 | 5.5 | 3.9 | 3.5 | 6.6 | 5.3 | 3.9 | 15 | 15 | 15 |
| y8 (1+) | 4.4 | 2.1 | 2.4 | 6.2 | 3.7 | 3.2 | 7.6 | 4.3 | 4 | 15 | 15 | 15 |
| y9 (1+) | 11.6 | 4.9 | 6 | 11.3 | 5.8 | 6.1 | 16.2 | 7.6 | 8.6 | 15 | 15 | 15 |
| sum | 2.6 | 2.6 | 1.4 | 4.4 | 3.1 | 2.9 | 5.1 | 4 | 3.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1544 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
71
Peptide End
84
CPTAC ID
CPTAC-1544
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 10.8 | 3.7 | 3.2 | 14.6 | 5.1 | 7.2 | 18.2 | 6.3 | 7.9 | 15 | 15 | 15 |
| y9 (1+) | 24.5 | 6.2 | 4 | 28.8 | 6.8 | 5.1 | 37.8 | 9.2 | 6.5 | 15 | 15 | 15 |
| y8 (1+) | 18.3 | 5.1 | 3 | 18.3 | 5.6 | 6.7 | 25.9 | 7.6 | 7.3 | 15 | 15 | 15 |
| b3 (1+) | 18.6 | 4.3 | 3.4 | 11.9 | 4.5 | 7.4 | 22.1 | 6.2 | 8.1 | 15 | 15 | 15 |
| sum | 10.9 | 3.3 | 2.3 | 9.7 | 3.9 | 6.4 | 14.6 | 5.1 | 6.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1545 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
LIHLEIKPAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
98
Peptide End
108
CPTAC ID
CPTAC-1545
Peptide Molecular Mass
1,301.8183
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 40.1 | 7.8 | 20.7 | 40.6 | 13.4 | 24.5 | 57.1 | 15.5 | 32.1 | 15 | 15 | 15 |
| y6 (1+) | 39.6 | 14.4 | 16.4 | 45.3 | 13.4 | 17.7 | 60.2 | 19.7 | 24.1 | 15 | 15 | 15 |
| y4 (1+) | 17.1 | 5.1 | 8.5 | 19.6 | 12.2 | 11.7 | 26 | 13.2 | 14.5 | 15 | 15 | 15 |
| y9 (2+) | 16 | 14.5 | 9.4 | 22.6 | 10.6 | 15 | 27.7 | 18 | 17.7 | 15 | 15 | 15 |
| sum | 5 | 8.5 | 5.8 | 14.5 | 9.6 | 11.9 | 15.3 | 12.8 | 13.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2940 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
71
Peptide End
84
CPTAC ID
CPTAC-2940
Peptide Molecular Mass
1,346.7194
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 5.4 | 7.3 | 3.3 | 5.8 | 9.5 | 4.5 | 7.9 | 12 | 5.6 | 15 | 15 | 15 |
| b3 (1+) | 3.8 | 7.6 | 3.2 | 5 | 8.9 | 3.8 | 6.3 | 11.7 | 5 | 15 | 15 | 15 |
| y8 (1+) | 5.9 | 10 | 3.9 | 5.4 | 10.6 | 5.3 | 8 | 14.6 | 6.6 | 15 | 15 | 15 |
| sum | 4.8 | 7.7 | 2.8 | 4.7 | 9.4 | 4.2 | 6.7 | 12.2 | 5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2939 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
LPSGVFSLEFQDFVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
325
Peptide End
340
CPTAC ID
CPTAC-2939
Peptide Molecular Mass
1,825.9251
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 6.2 | 3.1 | 5.7 | 7.2 | 6 | 13.7 | 9.5 | 6.8 | 14.8 | 15 | 15 | 15 |
| y4 (1+) | 4.8 | 2.6 | 4.2 | 5.2 | 5 | 10.6 | 7.1 | 5.6 | 11.4 | 15 | 15 | 15 |
| y10 (1+) | 12.1 | 3.4 | 5.5 | 16.4 | 5.9 | 13.4 | 20.4 | 6.8 | 14.5 | 15 | 15 | 15 |
| sum | 7.5 | 2.9 | 5.1 | 8.3 | 5.7 | 12.8 | 11.2 | 6.4 | 13.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-808 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
QLMVHAFIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
354
Peptide End
362
CPTAC ID
CPTAC-808
Peptide Molecular Mass
1,085.6056
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 7.4 | 7.4 | 6.4 | 5.7 | 7.5 | 7.4 | 9.3 | 10.5 | 9.8 | 15 | 15 | 15 |
| y5 (1+) | 13.8 | 6.6 | 3.7 | 13.7 | 6.2 | 4.5 | 19.4 | 9.1 | 5.8 | 15 | 15 | 15 |
| y6 (1+) | 12.8 | 11.2 | 10 | 16 | 12.3 | 10.2 | 20.5 | 16.6 | 14.3 | 15 | 15 | 15 |
| sum | 9.4 | 5.3 | 3.8 | 6.6 | 5.8 | 4.5 | 11.5 | 7.9 | 5.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-809 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K1
Peptide Sequence
VSHKPSGLVMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
85
Peptide End
96
CPTAC ID
CPTAC-809
Peptide Molecular Mass
1,280.7023
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 7 | 6.8 | 5.2 | 6.7 | 8.9 | 4.7 | 9.7 | 11.2 | 7 | 15 | 15 | 15 |
| y4 (1+) | 11.6 | 7.3 | 3.8 | 12.5 | 5.9 | 6 | 17.1 | 9.4 | 7.1 | 15 | 15 | 15 |
| y6 (1+) | 21.7 | 17.7 | 8 | 22.5 | 13.2 | 8.1 | 31.3 | 22.1 | 11.4 | 15 | 15 | 15 |
| y7 (1+) | 12.5 | 17.3 | 10.1 | 12.7 | 14.1 | 10.6 | 17.8 | 22.3 | 14.6 | 15 | 15 | 15 |
| y8 (1+) | 8.8 | 6 | 3.7 | 8.5 | 6.6 | 4.7 | 12.2 | 8.9 | 6 | 15 | 15 | 15 |
| y9 (1+) | 14.9 | 15.2 | 8.3 | 24.4 | 14.3 | 9.9 | 28.6 | 20.9 | 12.9 | 15 | 15 | 15 |
| sum | 6.9 | 3.6 | 2.2 | 6.2 | 4 | 2.6 | 9.3 | 5.4 | 3.4 | 15 | 15 | 15 |