MAP2K2, CPTAC-811 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP2K2 | MEK2, MKK2, PRKMK2 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 400 | 44424 |
| Protein Name |
|---|
| Dual specificity mitogen-activated protein kinase kinase 2 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-813: View additional SYMAPER data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MLARRKPVLP | ALTINPTIAE | GPSPTSEGAS | EANLVDLQKK | LEELELDEQQ |
| 60 | 70 | 80 | 90 | 100 |
| KKRLEAFLTQ | KAKVGELKDD | DFERISELGA | GNGGVVTKVQ | HRPSGLIMAR |
| 110 | 120 | 130 | 140 | 150 |
| KLIHLEIKPA | IRNQIIRELQ | VLHECNSPYI | VGFYGAFYSD | GEISICMEHM |
| 160 | 170 | 180 | 190 | 200 |
| DGGSLDQVLK | EAKRIPEEIL | GKVSIAVLRG | LAYLREKHQI | MHRDVKPSNI |
| 210 | 220 | 230 | 240 | 250 |
| LVNSRGEIKL | CDFGVSGQLI | DSMANSFVGT | RSYMAPERLQ | GTHYSVQSDI |
| 260 | 270 | 280 | 290 | 300 |
| WSMGLSLVEL | AVGRYPIPPP | DAKELEAIFG | RPVVDGEEGE | PHSISPRPRP |
| 310 | 320 | 330 | 340 | 350 |
| PGRPVSGHGM | DSRPAMAIFE | LLDYIVNEPP | PKLPNGVFTP | DFQEFVNKCL |
| 360 | 370 | 380 | 390 | 400 |
| IKNPAERADL | KMLTNHTFIK | RSEVEEVDFA | GWLCKTLRLN | QPGTPTRTAV |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1543 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K2
Peptide Sequence
ISELGAGNGGVVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
75
Peptide End
88
CPTAC ID
CPTAC-1543
Peptide Molecular Mass
1,300.6987
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 9.3 | 4.9 | 3.3 | 10.2 | 5.9 | 6.1 | 13.8 | 7.7 | 6.9 | 15 | 15 | 15 |
| y10 (1+) | 6.4 | 5.3 | 1.6 | 7.5 | 6 | 6.9 | 9.9 | 8 | 7.1 | 15 | 15 | 15 |
| y8 (1+) | 3.2 | 3.6 | 3.1 | 6.8 | 6.6 | 6.7 | 7.5 | 7.5 | 7.4 | 15 | 15 | 15 |
| b3 (1+) | 5.8 | 4.6 | 4.1 | 8 | 12.2 | 11.6 | 9.9 | 13 | 12.3 | 15 | 15 | 15 |
| sum | 3.2 | 3.3 | 1.5 | 5.2 | 6.9 | 6.9 | 6.1 | 7.6 | 7.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-811 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K2
Peptide Sequence
LNQPGTPTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
389
Peptide End
397
CPTAC ID
CPTAC-811
Peptide Molecular Mass
982.5196
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 5.3 | 2.8 | 2.6 | 5.2 | 2.8 | 2.9 | 7.4 | 4 | 3.9 | 15 | 15 | 15 |
| y5 (1+) | 6.6 | 2.1 | 5.1 | 6.5 | 2.3 | 5.5 | 9.3 | 3.1 | 7.5 | 15 | 15 | 15 |
| y6 (1+) | 1.2 | 1.1 | 1.7 | 1.6 | 1 | 1.8 | 2 | 1.5 | 2.5 | 15 | 15 | 15 |
| y7 (1+) | 6.6 | 4.1 | 4.4 | 6.4 | 3.5 | 5.5 | 9.2 | 5.4 | 7 | 15 | 15 | 15 |
| b3 (1+) | 6.4 | 3.9 | 3.5 | 10.3 | 5.3 | 3.5 | 12.1 | 6.6 | 4.9 | 15 | 15 | 15 |
| sum | 1.4 | 0.8 | 1.3 | 1.7 | 0.8 | 1.4 | 2.2 | 1.1 | 1.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1045 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K2
Peptide Modified Sequence
LNQPGTPT[+80.0]R
Modification Type
Phospho (ST)
Protein - Site of Modification
396
Peptide - Site of Modification
8
Peptide Start
389
Peptide End
397
CPTAC ID
CPTAC-1045
Peptide Molecular Mass
1,062.4859
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 7.9 | 9.4 | 6.1 | 10.3 | 10 | 6.3 | 13 | 13.7 | 8.8 | 15 | 15 | 15 |
| y3 (1+) | 42.7 | 23.4 | 14.6 | 43.2 | 22.8 | 18.2 | 60.7 | 32.7 | 23.3 | 15 | 15 | 15 |
| b3 (1+) | 27 | 10.4 | 10.7 | 26.9 | 11 | 12.3 | 38.1 | 15.1 | 16.3 | 15 | 15 | 15 |
| sum | 8.8 | 9.2 | 4.1 | 8.1 | 9.2 | 5.1 | 12 | 13 | 6.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-812 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K2
Peptide Sequence
LPNGVFTPDFQEFVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
333
Peptide End
348
CPTAC ID
CPTAC-812
Peptide Molecular Mass
1,850.9203
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 22.4 | 8.9 | 10.8 | 24.4 | 11.6 | 11.4 | 33.1 | 14.6 | 15.7 | 15 | 15 | 15 |
| y4 (1+) | 28.3 | 11.4 | 7.4 | 27.8 | 11.1 | 8.5 | 39.7 | 15.9 | 11.3 | 15 | 15 | 15 |
| y5 (1+) | 29.4 | 12.1 | 9.5 | 25.6 | 21.5 | 13.2 | 39 | 24.7 | 16.3 | 15 | 15 | 15 |
| y7 (1+) | 15.9 | 12.5 | 10.6 | 17.1 | 11.3 | 10.9 | 23.3 | 16.9 | 15.2 | 15 | 15 | 15 |
| y9 (1+) | 15.9 | 10.1 | 7.3 | 16.1 | 8.5 | 8 | 22.6 | 13.2 | 10.8 | 15 | 15 | 15 |
| y10 (1+) | 21.6 | 11.3 | 6.8 | 25.9 | 11.5 | 7.4 | 33.7 | 16.1 | 10 | 15 | 15 | 15 |
| y11 (1+) | 14.4 | 10 | 10.6 | 16.1 | 10.1 | 10.8 | 21.6 | 14.2 | 15.1 | 15 | 15 | 15 |
| b9 (1+) | 22.5 | 15.3 | 11.5 | 27.7 | 17.3 | 12.6 | 35.7 | 23.1 | 17.1 | 15 | 15 | 15 |
| b7 (1+) | 25.8 | 29.7 | 11.5 | 39.6 | 25.8 | 15 | 47.3 | 39.3 | 18.9 | 15 | 15 | 15 |
| b5 (1+) | 22.8 | 19.9 | 11.2 | 24.2 | 23.7 | 14.3 | 33.2 | 30.9 | 18.2 | 15 | 15 | 15 |
| b4 (1+) | 16.9 | 8.5 | 11 | 21.7 | 11.2 | 9.9 | 27.5 | 14.1 | 14.8 | 15 | 15 | 15 |
| sum | 2.8 | 4.7 | 3.2 | 4.2 | 4.1 | 3.2 | 5 | 6.2 | 4.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-813 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K2
Peptide Sequence
SYMAPER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
232
Peptide End
238
CPTAC ID
CPTAC-813
Peptide Molecular Mass
852.3800
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 16.3 | 7.1 | 6.4 | 11.2 | 6.7 | 5.7 | 19.8 | 9.8 | 8.6 | 15 | 15 | 15 |
| y4 (1+) | 7.7 | 5.8 | 4.5 | 8 | 6.7 | 3.8 | 11.1 | 8.9 | 5.9 | 15 | 15 | 15 |
| y5 (1+) | 6.8 | 4.3 | 3.4 | 6.1 | 4.2 | 4.4 | 9.1 | 6 | 5.6 | 15 | 15 | 15 |
| sum | 6.2 | 2.7 | 3.2 | 4.5 | 2.5 | 3.7 | 7.7 | 3.7 | 4.9 | 15 | 15 | 15 |