MAP2K6, CPTAC-820 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP2K6 | MEK6, MKK6, PRKMK6, SKK3 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 334 | 37492 |
| Protein Name |
|---|
| Dual specificity mitogen-activated protein kinase kinase 6 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-821: View additional GTDVASFVK data
CPTAC-2907: View additional HVPSGQIMAVK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MSQSKGKKRN | PGLKIPKEAF | EQPQTSSTPP | RDLDSKACIS | IGNQNFEVKA |
| 60 | 70 | 80 | 90 | 100 |
| DDLEPIMELG | RGAYGVVEKM | RHVPSGQIMA | VKRIRATVNS | QEQKRLLMDL |
| 110 | 120 | 130 | 140 | 150 |
| DISMRTVDCP | FTVTFYGALF | REGDVWICME | LMDTSLDKFY | KQVIDKGQTI |
| 160 | 170 | 180 | 190 | 200 |
| PEDILGKIAV | SIVKALEHLH | SKLSVIHRDV | KPSNVLINAL | GQVKMCDFGI |
| 210 | 220 | 230 | 240 | 250 |
| SGYLVDSVAK | TIDAGCKPYM | APERINPELN | QKGYSVKSDI | WSLGITMIEL |
| 260 | 270 | 280 | 290 | 300 |
| AILRFPYDSW | GTPFQQLKQV | VEEPSPQLPA | DKFSAEFVDF | TSQCLKKNSK |
| 310 | 320 | 330 | 334 | |
| ERPTYPELMQ | HPFFTLHESK | GTDVASFVKL | ILGD |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-820 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K6
Peptide Sequence
DVKPSNVLINALGQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
194
CPTAC ID
CPTAC-820
Peptide Molecular Mass
1,693.9727
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 10.9 | 7.5 | 9.6 | 13.2 | 8.2 | 11.5 | 17.1 | 11.1 | 15 | 15 | 15 | 15 |
| y5 (1+) | 12.4 | 8.5 | 9.1 | 23.9 | 7.8 | 10.9 | 26.9 | 11.5 | 14.2 | 15 | 15 | 15 |
| y6 (1+) | 25 | 14.1 | 13.6 | 24.7 | 14.7 | 12 | 35.1 | 20.4 | 18.1 | 15 | 15 | 15 |
| y7 (1+) | 15.8 | 11.6 | 10.1 | 16.8 | 16 | 10.2 | 23.1 | 19.8 | 14.4 | 15 | 15 | 15 |
| y8 (1+) | 20.9 | 11.8 | 8.2 | 22.3 | 10.3 | 8.2 | 30.6 | 15.7 | 11.6 | 15 | 15 | 15 |
| b8 (1+) | 26.2 | 15.8 | 17.8 | 33 | 16.5 | 17.2 | 42.1 | 22.8 | 24.8 | 15 | 15 | 15 |
| b7 (1+) | 13.8 | 8.1 | 6.4 | 17.2 | 10.2 | 5.4 | 22.1 | 13 | 8.4 | 15 | 15 | 15 |
| b6 (1+) | 17.3 | 9.4 | 8.6 | 16.4 | 13 | 8 | 23.8 | 16 | 11.7 | 15 | 15 | 15 |
| b5 (1+) | 26.7 | 17.8 | 19.1 | 26.5 | 23.4 | 23.1 | 37.6 | 29.4 | 30 | 15 | 15 | 15 |
| sum | 5.5 | 3.5 | 4.5 | 7.5 | 5.3 | 5.5 | 9.3 | 6.4 | 7.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-821 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K6
Peptide Sequence
GTDVASFVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
321
Peptide End
329
CPTAC ID
CPTAC-821
Peptide Molecular Mass
922.4760
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 14.3 | 12.4 | 8 | 16.5 | 8.7 | 9.4 | 21.8 | 15.1 | 12.3 | 15 | 15 | 15 |
| y4 (1+) | 14.7 | 7.8 | 6.4 | 12.2 | 8.2 | 6.3 | 19.1 | 11.3 | 9 | 15 | 15 | 15 |
| y5 (1+) | 10.2 | 4.5 | 5.9 | 11.4 | 5.4 | 5.1 | 15.3 | 7 | 7.8 | 15 | 15 | 15 |
| y6 (1+) | 11.2 | 6.3 | 7.8 | 11.9 | 6.8 | 6.8 | 16.3 | 9.3 | 10.3 | 15 | 15 | 15 |
| y7 (1+) | 10.2 | 6.4 | 4.3 | 10.5 | 7.5 | 4.3 | 14.6 | 9.9 | 6.1 | 15 | 15 | 15 |
| b4 (1+) | 17.7 | 6 | 11 | 16.8 | 8.9 | 12.1 | 24.4 | 10.7 | 16.4 | 15 | 15 | 15 |
| b3 (1+) | 21.4 | 6.7 | 10.8 | 20.2 | 13.6 | 11.9 | 29.4 | 15.2 | 16.1 | 15 | 15 | 15 |
| sum | 5.3 | 2.6 | 2.6 | 6.4 | 2.3 | 2.9 | 8.3 | 3.5 | 3.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2907 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP2K6
Peptide Sequence
HVPSGQIMAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
72
Peptide End
82
CPTAC ID
CPTAC-2907
Peptide Molecular Mass
1,165.6278
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b5 (1+) | 28 | 6 | 6.2 | 40.5 | 11.6 | 9.8 | 49.2 | 13.1 | 11.6 | 15 | 15 | 15 |
| y3 (1+) | 15.7 | 10.9 | 2.6 | 20.6 | 14.7 | 6 | 25.9 | 18.3 | 6.5 | 15 | 15 | 15 |
| b6 (1+) | 14.7 | 5.1 | 4.5 | 29.5 | 10.7 | 11 | 33 | 11.9 | 11.9 | 15 | 15 | 15 |
| sum | 10.5 | 7.2 | 3.6 | 23.2 | 13.1 | 8.3 | 25.5 | 14.9 | 9 | 15 | 15 | 15 |