MAP3K2, CPTAC-843 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K2 MAPKKK2, MEKK2
Sequence Length (AA) Molecular Weight (Da)
619 69741
Protein Name
Mitogen-activated protein kinase kinase kinase 2
Sources
UniProt PhosphoSitePlus ® GeneCards Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-844: View additional LSIIGPTSR data

10 20 30 40 50
MDDQQALNSI MQDLAVLHKA SRPALSLQET RKAKSSSPKK QNDVRVKFEH
60 70 80 90 100
RGEKRILQFP RPVKLEDLRS KAKIAFGQSM DLHYTNNELV IPLTTQDDLD
110 120 130 140 150
KAVELLDRSI HMKSLKILLV INGSTQATNL EPLPSLEDLD NTVFGAERKK
160 170 180 190 200
RLSIIGPTSR DRSSPPPGYI PDELHQVARN GSFTSINSEG EFIPESMDQM
210 220 230 240 250
LDPLSLSSPE NSGSGSCPSL DSPLDGESYP KSRMPRAQSY PDNHQEFSDY
260 270 280 290 300
DNPIFEKFGK GGTYPRRYHV SYHHQEYNDG RKTFPRARRT QGTSLRSPVS
310 320 330 340 350
FSPTDHSLST SSGSSIFTPE YDDSRIRRRG SDIDNPTLTV MDISPPSRSP
360 370 380 390 400
RAPTNWRLGK LLGQGAFGRV YLCYDVDTGR ELAVKQVQFD PDSPETSKEV
410 420 430 440 450
NALECEIQLL KNLLHERIVQ YYGCLRDPQE KTLSIFMEYM PGGSIKDQLK
460 470 480 490 500
AYGALTENVT RKYTRQILEG VHYLHSNMIV HRDIKGANIL RDSTGNVKLG
510 520 530 540 550
DFGASKRLQT ICLSGTGMKS VTGTPYWMSP EVISGEGYGR KADIWSVACT
560 570 580 590 600
VVEMLTEKPP WAEFEAMAAI FKIATQPTNP KLPPHVSDYT RDFLKRIFVE
610 619
AKLRPSADEL LRHMFVHYH

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-843 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAP3K2

Peptide Sequence

AQSYPDNHQEFSDYDNPIFEK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

237

Peptide End

257

CPTAC ID

CPTAC-843

Peptide Molecular Mass

2,543.0877

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Pooled patient derived xenograft breast tumor digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TripleTOF 5600+ (SCIEX)

Internal Standard

Peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

2DPlus nano-LC (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

200 µm x 15 cm

Flow Rate

800 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y3 (1+) | 16 | 10.3 | 7.7 | 16.5 | 8.4 | 8.5 | 23 | 13.3 | 11.5 | 15 | 15 | 15 | | y5 (1+) | 14.2 | 5.8 | 7.6 | 22.5 | 6.9 | 5.3 | 26.6 | 9 | 9.3 | 15 | 15 | 15 | | y6 (1+) | 24 | 7.8 | 14.6 | 29 | 8.6 | 14.2 | 37.6 | 11.6 | 20.4 | 15 | 15 | 15 | | y7 (1+) | 26.3 | 17.5 | 16.5 | 33.1 | 16.2 | 21.2 | 42.3 | 23.8 | 26.9 | 15 | 15 | 15 | | y9 (1+) | 24 | 9.9 | 20.7 | 27.6 | 16.4 | 22.3 | 36.6 | 19.2 | 30.4 | 15 | 15 | 15 | | y11 (1+) | 28.1 | 8.8 | 13.2 | 33.2 | 19.1 | 13 | 43.5 | 21 | 18.5 | 15 | 15 | 15 | | sum | 9.6 | 4.7 | 5.1 | 11.1 | 4.3 | 3.2 | 14.7 | 6.4 | 6 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-2983 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAP3K2

Peptide Sequence

AQSYPDNHQEFSDYDNPIFEK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

237

Peptide End

257

CPTAC ID

CPTAC-2983

Peptide Molecular Mass

2,543.0877

Species

Homo Sapiens

Assay Type

Direct PRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher, Q-Exactive

Internal Standard

25 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 µm x 50 cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (1+) | 9 | 5.1 | 18 | 10.2 | 11.7 | 24 | 13.6 | 12.8 | 30 | 15 | 15 | 15 | | b3 (1+) | 9.1 | 5.4 | 4.3 | 8.9 | 5.2 | 4.1 | 12.7 | 7.5 | 5.9 | 15 | 15 | 15 | | y3 (1+) | 8.9 | 5.4 | 2.3 | 7.2 | 6 | 2.5 | 11.4 | 8.1 | 3.4 | 15 | 15 | 15 | | sum | 7 | 4.7 | 6.3 | 6.4 | 6.4 | 7.4 | 9.5 | 7.9 | 9.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-844 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAP3K2

Peptide Sequence

LSIIGPTSR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

152

Peptide End

160

CPTAC ID

CPTAC-844

Peptide Molecular Mass

942.5498

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Pooled patient derived xenograft breast tumor digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TripleTOF 5600+ (SCIEX)

Internal Standard

Peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

2DPlus nano-LC (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

200 µm x 15 cm

Flow Rate

800 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 7.8 | 4.4 | 4.1 | 7.2 | 5.5 | 4.1 | 10.6 | 7 | 5.8 | 15 | 15 | 15 | | y5 (1+) | 2.7 | 2.1 | 2.4 | 3 | 1.9 | 2.9 | 4 | 2.8 | 3.8 | 15 | 15 | 15 | | y6 (1+) | 2.2 | 2.2 | 2 | 3.2 | 2.5 | 2.9 | 3.9 | 3.3 | 3.5 | 15 | 15 | 15 | | y7 (1+) | 10.1 | 2.5 | 2.7 | 8.4 | 3.1 | 3.2 | 13.1 | 4 | 4.2 | 15 | 15 | 15 | | y8 (1+) | 4.3 | 4.5 | 2.4 | 8.2 | 3.6 | 3.3 | 9.3 | 5.8 | 4.1 | 15 | 15 | 15 | | sum | 1.5 | 1.7 | 1.7 | 2.4 | 1.8 | 2.4 | 2.8 | 2.5 | 2.9 | 15 | 15 | 15 |

Additional Resources and Comments