MAP3K7, CPTAC-853 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-2812: View additional IAATTAYSKPK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MSTASAASSS | SSSSAGEMIE | APSQVLNFEE | IDYKEIEVEE | VVGRGAFGVV |
| 60 | 70 | 80 | 90 | 100 |
| CKAKWRAKDV | AIKQIESESE | RKAFIVELRQ | LSRVNHPNIV | KLYGACLNPV |
| 110 | 120 | 130 | 140 | 150 |
| CLVMEYAEGG | SLYNVLHGAE | PLPYYTAAHA | MSWCLQCSQG | VAYLHSMQPK |
| 160 | 170 | 180 | 190 | 200 |
| ALIHRDLKPP | NLLLVAGGTV | LKICDFGTAC | DIQTHMTNNK | GSAAWMAPEV |
| 210 | 220 | 230 | 240 | 250 |
| FEGSNYSEKC | DVFSWGIILW | EVITRRKPFD | EIGGPAFRIM | WAVHNGTRPP |
| 260 | 270 | 280 | 290 | 300 |
| LIKNLPKPIE | SLMTRCWSKD | PSQRPSMEEI | VKIMTHLMRY | FPGADEPLQY |
| 310 | 320 | 330 | 340 | 350 |
| PCQYSDEGQS | NSATSTGSFM | DIASTNTSNK | SDTNMEQVPA | TNDTIKRLES |
| 360 | 370 | 380 | 390 | 400 |
| KLLKNQAKQQ | SESGRLSLGA | SRGSSVESLP | PTSEGKRMSA | DMSEIEARIA |
| 410 | 420 | 430 | 440 | 450 |
| ATTAYSKPKR | GHRKTASFGN | ILDVPEIVIS | GNGQPRRRSI | QDLTVTGTEP |
| 460 | 470 | 480 | 490 | 500 |
| GQVSSRSSSP | SVRMITTSGP | TSEKPTRSHP | WTPDDSTDTN | GSDNSIPMAY |
| 510 | 520 | 530 | 540 | 550 |
| LTLDHQLQPL | APCPNSKESM | AVFEQHCKMA | QEYMKVQTEI | ALLLQRKQEL |
| 560 | 570 | 580 | 590 | 600 |
| VAELDQDEKD | QQNTSRLVQE | HKKLLDENKS | LSTYYQQCKK | QLEVIRSQQQ |
| 606 | ||||
| KRQGTS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-853 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Sequence
DLKPPNLLLVAGGTVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
156
Peptide End
172
CPTAC ID
CPTAC-853
Peptide Molecular Mass
1,747.0608
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 7.5 | 13.9 | 8.4 | 12.4 | 14.2 | 9.3 | 14.5 | 19.9 | 12.5 | 15 | 15 | 15 |
| y7 (1+) | 17.9 | 11.4 | 7.9 | 18 | 10 | 8.3 | 25.4 | 15.2 | 11.5 | 15 | 15 | 15 |
| y8 (1+) | 17.9 | 12.4 | 7.4 | 15.5 | 14.5 | 8.6 | 23.7 | 19.1 | 11.3 | 15 | 15 | 15 |
| y9 (1+) | 29.4 | 14.1 | 7.4 | 24.9 | 15.8 | 11 | 38.5 | 21.2 | 13.3 | 15 | 15 | 15 |
| b8 (1+) | 28.3 | 13.8 | 10.5 | 24.5 | 12 | 10.6 | 37.4 | 18.3 | 14.9 | 15 | 15 | 15 |
| b7 (1+) | 12.5 | 8.8 | 8.2 | 11.4 | 11.5 | 8.1 | 16.9 | 14.5 | 11.5 | 15 | 15 | 15 |
| b6 (1+) | 24.9 | 7.6 | 7.4 | 24.7 | 11.5 | 7.8 | 35.1 | 13.8 | 10.8 | 15 | 15 | 15 |
| b4 (1+) | 42.1 | 23.9 | 14.1 | 37 | 24.6 | 14.9 | 56 | 34.3 | 20.5 | 15 | 15 | 15 |
| sum | 9.1 | 5.8 | 2.9 | 8.5 | 5.7 | 2.6 | 12.5 | 8.1 | 3.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3093 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Modified Sequence
GAFGVVC[+57.0]K
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
52
Peptide - Site of Modification
7
Peptide Start
45
Peptide End
52
CPTAC ID
CPTAC-3093
Peptide Molecular Mass
836.4215
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 3.4 | 3.4 | 4.4 | 4.7 | 3.6 | 7.9 | 5.8 | 5 | 9 | 15 | 15 | 15 |
| y3 (1+) | 2.9 | 4 | 5.1 | 4.2 | 5.2 | 7.5 | 5.1 | 6.6 | 9.1 | 15 | 15 | 15 |
| y4 (1+) | 6.1 | 3.2 | 5.9 | 6.5 | 3.2 | 6 | 8.9 | 4.5 | 8.4 | 15 | 15 | 15 |
| sum | 3.3 | 3.4 | 4.7 | 4.4 | 3.8 | 7.4 | 5.5 | 5.1 | 8.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2812 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Sequence
IAATTAYSKPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
399
Peptide End
409
CPTAC ID
CPTAC-2812
Peptide Molecular Mass
1,149.6394
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 5.2 | 3.7 | 6.2 | 6.7 | 3.8 | 7.2 | 8.5 | 5.3 | 9.5 | 15 | 15 | 15 |
| y5 (1+) | 3.5 | 3 | 4.1 | 5.1 | 3.3 | 7.5 | 6.2 | 4.5 | 8.5 | 15 | 15 | 15 |
| y4 (1+) | 5.6 | 5.1 | 4.8 | 6.9 | 5.3 | 6.9 | 8.9 | 7.4 | 8.4 | 15 | 15 | 15 |
| sum | 2.9 | 3.3 | 4.6 | 4.6 | 3.4 | 7 | 5.4 | 4.7 | 8.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-854 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Sequence
IAATTGNGQPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
11
CPTAC ID
CPTAC-854
Peptide Molecular Mass
1,084.5625
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 13 | 10.3 | 4.1 | 16.2 | 10.1 | 4.3 | 20.8 | 14.4 | 5.9 | 15 | 15 | 15 |
| y5 (1+) | 10.8 | 8.9 | 5 | 9.4 | 9.7 | 5.1 | 14.3 | 13.2 | 7.1 | 15 | 15 | 15 |
| y6 (1+) | 5 | 3.7 | 2.1 | 6.3 | 3.7 | 3.6 | 8 | 5.2 | 4.2 | 15 | 15 | 15 |
| y7 (1+) | 6 | 4.5 | 4.6 | 6.5 | 3.7 | 4.9 | 8.8 | 5.8 | 6.7 | 15 | 15 | 15 |
| y8 (1+) | 4.4 | 4.4 | 5.5 | 5.9 | 5.3 | 3.1 | 7.4 | 6.9 | 6.3 | 15 | 15 | 15 |
| y9 (1+) | 5.6 | 4.9 | 2.6 | 5.3 | 6.2 | 2.7 | 7.7 | 7.9 | 3.7 | 15 | 15 | 15 |
| y10 (1+) | 6.7 | 4.2 | 4 | 7.8 | 4.5 | 5.4 | 10.3 | 6.2 | 6.7 | 15 | 15 | 15 |
| sum | 2.4 | 2.5 | 1.1 | 2.7 | 3 | 1.3 | 3.6 | 3.9 | 1.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-855 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Modified Sequence
MS[+80.0]ADMSEIEAR
Modification Type
Phospho (ST)
Protein - Site of Modification
389
Peptide - Site of Modification
2
Peptide Start
388
Peptide End
398
CPTAC ID
CPTAC-855
Peptide Molecular Mass
1,318.4935
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 38.3 | 16.2 | 26.6 | 40.8 | 17 | 27.5 | 56 | 23.5 | 38.3 | 15 | 15 | 15 |
| y5 (1+) | 34.8 | 16 | 14.4 | 28.5 | 19 | 13.9 | 45 | 24.8 | 20 | 15 | 15 | 15 |
| y6 (1+) | 16 | 6.7 | 5.9 | 13.3 | 8.9 | 6.8 | 20.8 | 11.1 | 9 | 15 | 15 | 15 |
| y7 (1+) | 15.8 | 12.4 | 5.8 | 14.2 | 12.4 | 7.9 | 21.2 | 17.5 | 9.8 | 15 | 15 | 15 |
| sum | 9.3 | 6.6 | 5.1 | 8.1 | 7.8 | 4.8 | 12.3 | 10.2 | 7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-856 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Sequence
MSADMSEIEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
388
Peptide End
398
CPTAC ID
CPTAC-856
Peptide Molecular Mass
1,238.5271
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 11.1 | 7 | 5.7 | 9.3 | 4.8 | 5.9 | 14.5 | 8.5 | 8.2 | 15 | 15 | 15 |
| y5 (1+) | 10 | 10.8 | 4.3 | 13 | 11.7 | 4.8 | 16.4 | 15.9 | 6.4 | 15 | 15 | 15 |
| y6 (1+) | 6.6 | 4.2 | 4.8 | 4.7 | 4.5 | 4.9 | 8.1 | 6.2 | 6.9 | 15 | 15 | 15 |
| y7 (1+) | 8.6 | 4.3 | 2.4 | 7.2 | 5.8 | 3.2 | 11.2 | 7.2 | 4 | 15 | 15 | 15 |
| y8 (1+) | 8.5 | 4.3 | 4 | 8.8 | 6.1 | 4.3 | 12.2 | 7.5 | 5.9 | 15 | 15 | 15 |
| y9 (1+) | 10.8 | 5.1 | 6.6 | 10.4 | 5 | 7.6 | 15 | 7.1 | 10.1 | 15 | 15 | 15 |
| y10 (1+) | 10.9 | 4.6 | 5.9 | 9.5 | 6.3 | 8 | 14.5 | 7.8 | 9.9 | 15 | 15 | 15 |
| b4 (1+) | 18.3 | 12.1 | 8.3 | 17.2 | 13.3 | 8.3 | 25.1 | 18 | 11.7 | 15 | 15 | 15 |
| sum | 3 | 1.6 | 1.8 | 3.2 | 1.7 | 2 | 4.4 | 2.3 | 2.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1048 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K7
Peptide Modified Sequence
S[+80.0]IQDLTVTGTEPGQVSSR
Modification Type
Phospho (ST)
Protein - Site of Modification
439
Peptide - Site of Modification
1
Peptide Start
439
Peptide End
456
CPTAC ID
CPTAC-1048
Peptide Molecular Mass
1,953.9045
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 14.4 | 6 | 6.8 | 16.5 | 7.9 | 6.3 | 21.9 | 9.9 | 9.3 | 15 | 15 | 15 |
| y6 (1+) | 32.9 | 9.2 | 5.6 | 25.7 | 9 | 7.6 | 41.7 | 12.9 | 9.4 | 15 | 15 | 15 |
| y4 (1+) | 28.6 | 13.1 | 9.9 | 38.9 | 14.8 | 12.3 | 48.3 | 19.8 | 15.8 | 15 | 15 | 15 |
| sum | 9.9 | 4.4 | 5.8 | 13.6 | 6.5 | 5.1 | 16.8 | 7.8 | 7.7 | 15 | 15 | 15 |