MAP3K8, CPTAC-858 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP3K8 | COT, ESTF |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 467 | 52925 |
| Protein Name |
|---|
| Mitogen-activated protein kinase kinase kinase 8 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-858: View additional IEASLER data
CPTAC-859: View additional NIGSDFIPR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MEYMSTGSDN | KEEIDLLIKH | LNVSDVIDIM | ENLYASEEPA | VYEPSLMTMC |
| 60 | 70 | 80 | 90 | 100 |
| QDSNQNDERS | KSLLLSGQEV | PWLSSVRYGT | VEDLLAFANH | ISNTAKHFYG |
| 110 | 120 | 130 | 140 | 150 |
| QRPQESGILL | NMVITPQNGR | YQIDSDVLLI | PWKLTYRNIG | SDFIPRGAFG |
| 160 | 170 | 180 | 190 | 200 |
| KVYLAQDIKT | KKRMACKLIP | VDQFKPSDVE | IQACFRHENI | AELYGAVLWG |
| 210 | 220 | 230 | 240 | 250 |
| ETVHLFMEAG | EGGSVLEKLE | SCGPMREFEI | IWVTKHVLKG | LDFLHSKKVI |
| 260 | 270 | 280 | 290 | 300 |
| HHDIKPSNIV | FMSTKAVLVD | FGLSVQMTED | VYFPKDLRGT | EIYMSPEVIL |
| 310 | 320 | 330 | 340 | 350 |
| CRGHSTKADI | YSLGATLIHM | QTGTPPWVKR | YPRSAYPSYL | YIIHKQAPPL |
| 360 | 370 | 380 | 390 | 400 |
| EDIADDCSPG | MRELIEASLE | RNPNHRPRAA | DLLKHEALNP | PREDQPRCQS |
| 410 | 420 | 430 | 440 | 450 |
| LDSALLERKR | LLSRKELELP | ENIADSSCTG | STEESEMLKR | QRSLYIDLGA |
| 460 | 467 | |||
| LAGYFNLVRG | PPTLEYG |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-858 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K8
Peptide Sequence
IEASLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
365
Peptide End
371
CPTAC ID
CPTAC-858
Peptide Molecular Mass
816.4341
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 13.3 | 7.1 | 9.2 | 14.9 | 10.9 | 10.2 | 20 | 13 | 13.7 | 15 | 15 | 15 |
| y5 (1+) | 6.4 | 7.4 | 7.2 | 8 | 7.1 | 6.9 | 10.2 | 10.3 | 10 | 15 | 15 | 15 |
| y6 (1+) | 11.5 | 6.5 | 6.5 | 12 | 7.3 | 6.6 | 16.6 | 9.8 | 9.3 | 15 | 15 | 15 |
| sum | 7.7 | 5.3 | 6.6 | 8.2 | 5.2 | 6.4 | 11.2 | 7.4 | 9.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-859 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP3K8
Peptide Sequence
NIGSDFIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
138
Peptide End
146
CPTAC ID
CPTAC-859
Peptide Molecular Mass
1,017.5243
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 19.9 | 9.8 | 7.7 | 22.1 | 9.8 | 11.2 | 29.7 | 13.9 | 13.6 | 15 | 15 | 15 |
| y4 (1+) | 4.3 | 5.1 | 4.5 | 9.2 | 4.6 | 5.1 | 10.2 | 6.9 | 6.8 | 15 | 15 | 15 |
| y5 (1+) | 20.4 | 19.7 | 10 | 18.4 | 14.9 | 12.8 | 27.5 | 24.7 | 16.2 | 15 | 15 | 15 |
| y6 (1+) | 8 | 11.3 | 7.4 | 9 | 7.9 | 6.9 | 12 | 13.8 | 10.1 | 15 | 15 | 15 |
| y7 (1+) | 6.4 | 2.6 | 2.8 | 4.7 | 3.9 | 3.6 | 7.9 | 4.7 | 4.6 | 15 | 15 | 15 |
| sum | 4.9 | 3.1 | 2.1 | 4.7 | 3.5 | 2.6 | 6.8 | 4.7 | 3.3 | 15 | 15 | 15 |