MAP4K2, CPTAC-862 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP4K2 | GCK, RAB8IP |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 820 | 91556 |
| Protein Name |
|---|
| Mitogen-activated protein kinase kinase kinase kinase 2 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MALLRDVSLQ | DPRDRFELLQ | RVGAGTYGDV | YKARDTVTSE | LAAVKIVKLD |
| 60 | 70 | 80 | 90 | 100 |
| PGDDISSLQQ | EITILRECRH | PNVVAYIGSY | LRNDRLWICM | EFCGGGSLQE |
| 110 | 120 | 130 | 140 | 150 |
| IYHATGPLEE | RQIAYVCREA | LKGLHHLHSQ | GKIHRDIKGA | NLLLTLQGDV |
| 160 | 170 | 180 | 190 | 200 |
| KLADFGVSGE | LTASVAKRRS | FIGTPYWMAP | EVAAVERKGG | YNELCDVWAL |
| 210 | 220 | 230 | 240 | 250 |
| GITAIELGEL | QPPLFHLHPM | RALMLMSKSS | FQPPKLRDKT | RWTQNFHHFL |
| 260 | 270 | 280 | 290 | 300 |
| KLALTKNPKK | RPTAEKLLQH | PFTTQQLPRA | LLTQLLDKAS | DPHLGTPSPE |
| 310 | 320 | 330 | 340 | 350 |
| DCELETYDMF | PDTIHSRGQH | GPAERTPSEI | QFHQVKFGAP | RRKETDPLNE |
| 360 | 370 | 380 | 390 | 400 |
| PWEEEWTLLG | KEELSGSLLQ | SVQEALEERS | LTIRSASEFQ | ELDSPDDTMG |
| 410 | 420 | 430 | 440 | 450 |
| TIKRAPFLGP | LPTDPPAEEP | LSSPPGTLPP | PPSGPNSSPL | LPTAWATMKQ |
| 460 | 470 | 480 | 490 | 500 |
| REDPERSSCH | GLPPTPKVHM | GACFSKVFNG | CPLRIHAAVT | WIHPVTRDQF |
| 510 | 520 | 530 | 540 | 550 |
| LVVGAEEGIY | TLNLHELHED | TLEKLISHRC | SWLYCVNNVL | LSLSGKSTHI |
| 560 | 570 | 580 | 590 | 600 |
| WAHDLPGLFE | QRRLQQQVPL | SIPTNRLTQR | IIPRRFALST | KIPDTKGCLQ |
| 610 | 620 | 630 | 640 | 650 |
| CRVVRNPYTG | ATFLLAALPT | SLLLLQWYEP | LQKFLLLKNF | SSPLPSPAGM |
| 660 | 670 | 680 | 690 | 700 |
| LEPLVLDGKE | LPQVCVGAEG | PEGPGCRVLF | HVLPLEAGLT | PDILIPPEGI |
| 710 | 720 | 730 | 740 | 750 |
| PGSAQQVIQV | DRDTILVSFE | RCVRIVNMQG | EPTATLAPEL | TFDFPIETVV |
| 760 | 770 | 780 | 790 | 800 |
| CLQDSVLAFW | SHGMQGRSLD | TNEVTQEITD | ETRIFRVLGA | HRDIILESIP |
| 810 | 820 | |||
| TDNPEAHSNL | YILTGHQSTY |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-862 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K2
Peptide Sequence
IHAAVTWIHPVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
485
Peptide End
497
CPTAC ID
CPTAC-862
Peptide Molecular Mass
1,499.8361
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 6.3 | 4.2 | 5.2 | 5.9 | 4.8 | 6.1 | 8.6 | 6.4 | 8 | 15 | 15 | 15 |
| b4 (1+) | 15.5 | 6.9 | 7.8 | 15.8 | 7.1 | 9 | 22.1 | 9.9 | 11.9 | 15 | 15 | 15 |
| b3 (1+) | 7.1 | 7.6 | 5.7 | 9.7 | 7.9 | 5.7 | 12 | 11 | 8.1 | 15 | 15 | 15 |
| sum | 5.8 | 3.2 | 4 | 6.2 | 3.8 | 4.3 | 8.5 | 5 | 5.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2865 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K2
Peptide Sequence
LQQQVPLSIPTNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
564
Peptide End
576
CPTAC ID
CPTAC-2865
Peptide Molecular Mass
1,492.8362
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 5.2 | 3.6 | 3.2 | 6.7 | 3.1 | 3.9 | 8.5 | 4.8 | 5 | 15 | 15 | 15 |
| y4 (1+) | 4.3 | 3 | 3.3 | 5.5 | 2.6 | 3.7 | 7 | 4 | 5 | 15 | 15 | 15 |
| y9 (1+) | 6.6 | 4.4 | 3.3 | 7.4 | 3.6 | 4.6 | 9.9 | 5.7 | 5.7 | 15 | 15 | 15 |
| sum | 5 | 3.5 | 3.2 | 6.3 | 2.9 | 3.8 | 8 | 4.5 | 5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2864 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K2
Peptide Sequence
SLDTNEVTQEITDETR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
768
Peptide End
783
CPTAC ID
CPTAC-2864
Peptide Molecular Mass
1,849.8541
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 16 | 7.4 | 4.3 | 16.3 | 8.5 | 5 | 22.8 | 11.3 | 6.6 | 15 | 15 | 15 |
| y9 (1+) | 15.4 | 6.5 | 5.8 | 15.6 | 8 | 5.6 | 21.9 | 10.3 | 8.1 | 15 | 15 | 15 |
| y10 (1+) | 16.2 | 6.2 | 5.8 | 16 | 7.4 | 5.6 | 22.8 | 9.7 | 8.1 | 15 | 15 | 15 |
| sum | 15.5 | 6.5 | 5.5 | 15.7 | 7.8 | 5.3 | 22.1 | 10.2 | 7.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-863 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K2
Peptide Modified Sequence
VFNGC[+57.0]PLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
481
Peptide - Site of Modification
5
Peptide Start
477
Peptide End
484
CPTAC ID
CPTAC-863
Peptide Molecular Mass
961.4804
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 10.8 | 4 | 3.8 | 14.1 | 9 | 6.3 | 17.8 | 9.8 | 7.4 | 15 | 15 | 15 |
| y4 (1+) | 11.2 | 8.7 | 7 | 9.6 | 8.8 | 5.1 | 14.8 | 12.4 | 8.7 | 15 | 15 | 15 |
| y5 (1+) | 7.5 | 3.5 | 2 | 7.7 | 3.5 | 4.7 | 10.7 | 4.9 | 5.1 | 15 | 15 | 15 |
| y6 (1+) | 3.5 | 1.9 | 3.5 | 4.9 | 2.1 | 3 | 6 | 2.8 | 4.6 | 15 | 15 | 15 |
| y7 (1+) | 7.2 | 3.9 | 6 | 6.3 | 5.1 | 6 | 9.6 | 6.4 | 8.5 | 15 | 15 | 15 |
| sum | 4 | 1.6 | 1.2 | 3.5 | 2.3 | 2 | 5.3 | 2.8 | 2.3 | 15 | 15 | 15 |