MAP4K3, CPTAC-864 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MAP4K3 | RAB8IPL1 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 894 | 101316 |
| Protein Name |
|---|
| Mitogen-activated protein kinase kinase kinase kinase 3 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MNPGFDLSRR | NPQEDFELIQ | RIGSGTYGDV | YKARNVNTGE | LAAIKVIKLE |
| 60 | 70 | 80 | 90 | 100 |
| PGEDFAVVQQ | EIIMMKDCKH | PNIVAYFGSY | LRRDKLWICM | EFCGGGSLQD |
| 110 | 120 | 130 | 140 | 150 |
| IYHVTGPLSE | LQIAYVSRET | LQGLYYLHSK | GKMHRDIKGA | NILLTDNGHV |
| 160 | 170 | 180 | 190 | 200 |
| KLADFGVSAQ | ITATIAKRKS | FIGTPYWMAP | EVAAVERKGG | YNQLCDLWAV |
| 210 | 220 | 230 | 240 | 250 |
| GITAIELAEL | QPPMFDLHPM | RALFLMTKSN | FQPPKLKDKM | KWSNSFHHFV |
| 260 | 270 | 280 | 290 | 300 |
| KMALTKNPKK | RPTAEKLLQH | PFVTQHLTRS | LAIELLDKVN | NPDHSTYHDF |
| 310 | 320 | 330 | 340 | 350 |
| DDDDPEPLVA | VPHRIHSTSR | NVREEKTRSE | ITFGQVKFDP | PLRKETEPHH |
| 360 | 370 | 380 | 390 | 400 |
| ELPDSDGFLD | SSEEIYYTAR | SNLDLQLEYG | QGHQGGYFLG | ANKSLLKSVE |
| 410 | 420 | 430 | 440 | 450 |
| EELHQRGHVA | HLEDDEGDDD | ESKHSTLKAK | IPPPLPPKPK | SIFIPQEMHS |
| 460 | 470 | 480 | 490 | 500 |
| TEDENQGTIK | RCPMSGSPAK | PSQVPPRPPP | PRLPPHKPVA | LGNGMSSFQL |
| 510 | 520 | 530 | 540 | 550 |
| NGERDGSLCQ | QQNEHRGTNL | SRKEKKDVPK | PISNGLPPTP | KVHMGACFSK |
| 560 | 570 | 580 | 590 | 600 |
| VFNGCPLKIH | CASSWINPDT | RDQYLIFGAE | EGIYTLNLNE | LHETSMEQLF |
| 610 | 620 | 630 | 640 | 650 |
| PRRCTWLYVM | NNCLLSISGK | ASQLYSHNLP | GLFDYARQMQ | KLPVAIPAHK |
| 660 | 670 | 680 | 690 | 700 |
| LPDRILPRKF | SVSAKIPETK | WCQKCCVVRN | PYTGHKYLCG | ALQTSIVLLE |
| 710 | 720 | 730 | 740 | 750 |
| WVEPMQKFML | IKHIDFPIPC | PLRMFEMLVV | PEQEYPLVCV | GVSRGRDFNQ |
| 760 | 770 | 780 | 790 | 800 |
| VVRFETVNPN | STSSWFTESD | TPQTNVTHVT | QLERDTILVC | LDCCIKIVNL |
| 810 | 820 | 830 | 840 | 850 |
| QGRLKSSRKL | SSELTFDFQI | ESIVCLQDSV | LAFWKHGMQG | RSFRSNEVTQ |
| 860 | 870 | 880 | 890 | 894 |
| EISDSTRIFR | LLGSDRVVVL | ESRPTDNPTA | NSNLYILAGH | ENSY |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-3018 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K3
Peptide Sequence
ASQLYSHNLPGLFDYAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
621
Peptide End
637
CPTAC ID
CPTAC-3018
Peptide Molecular Mass
1,950.9588
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 7 | 5.7 | 4.4 | 13.6 | 6.3 | 4.9 | 15.3 | 8.5 | 6.6 | 15 | 15 | 15 |
| y3 (1+) | 7.9 | 6.4 | 3.8 | 12.7 | 7.4 | 4.4 | 15 | 9.8 | 5.8 | 15 | 15 | 15 |
| y8 (1+) | 6.4 | 6.5 | 4.1 | 15.5 | 7.1 | 4.7 | 16.8 | 9.6 | 6.2 | 15 | 15 | 15 |
| sum | 5.8 | 6.1 | 4 | 14.3 | 6.8 | 4.6 | 15.4 | 9.1 | 6.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3017 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K3
Peptide Sequence
ETLQGLYYLHSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
119
Peptide End
130
CPTAC ID
CPTAC-3017
Peptide Molecular Mass
1,450.7456
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 8.2 | 6.6 | 5.5 | 10.2 | 7.4 | 6 | 13.1 | 9.9 | 8.1 | 15 | 15 | 15 |
| y5 (1+) | 9.2 | 6 | 6.1 | 13.1 | 8.6 | 7.1 | 16 | 10.5 | 9.4 | 15 | 15 | 15 |
| y6 (1+) | 9 | 6.6 | 6.8 | 11.4 | 9.2 | 7.7 | 14.5 | 11.3 | 10.3 | 15 | 15 | 15 |
| sum | 8.3 | 6.3 | 5.9 | 11.4 | 8.3 | 6.7 | 14.1 | 10.4 | 8.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-864 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K3
Peptide Modified Sequence
HIDFPIPC[+57.0]PLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
720
Peptide - Site of Modification
8
Peptide Start
713
Peptide End
723
CPTAC ID
CPTAC-864
Peptide Molecular Mass
1,363.7071
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 12.3 | 8.9 | 5.2 | 14.9 | 8.7 | 5.5 | 19.3 | 12.4 | 7.6 | 15 | 15 | 15 |
| y4 (1+) | 13.8 | 8.3 | 6.7 | 17.4 | 8.8 | 9.2 | 22.2 | 12.1 | 11.4 | 15 | 15 | 15 |
| y5 (1+) | 8.2 | 2.8 | 2.4 | 8 | 3.4 | 2.5 | 11.5 | 4.4 | 3.5 | 15 | 15 | 15 |
| b4 (1+) | 6.5 | 4.5 | 3.2 | 8.2 | 4.8 | 3.6 | 10.5 | 6.6 | 4.8 | 15 | 15 | 15 |
| b3 (1+) | 11 | 3.3 | 2.5 | 10.7 | 5.2 | 3.1 | 15.3 | 6.2 | 4 | 15 | 15 | 15 |
| sum | 3.1 | 3 | 1.9 | 4.8 | 3.5 | 1.8 | 5.7 | 4.6 | 2.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-865 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAP4K3
Peptide Sequence
LLQHPFVTQHLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
267
Peptide End
279
CPTAC ID
CPTAC-865
Peptide Molecular Mass
1,588.8838
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 9 | 6.3 | 5.9 | 9.9 | 8.2 | 7.2 | 13.4 | 10.3 | 9.3 | 15 | 15 | 15 |
| y4 (1+) | 13.2 | 6.5 | 4.5 | 8.7 | 6.2 | 4.1 | 15.8 | 9 | 6.1 | 15 | 15 | 15 |
| y5 (1+) | 8.4 | 11.6 | 5.3 | 9.2 | 11.2 | 7.6 | 12.5 | 16.1 | 9.3 | 15 | 15 | 15 |
| y6 (1+) | 10.4 | 5.4 | 6.1 | 9.6 | 6.5 | 5.9 | 14.2 | 8.5 | 8.5 | 15 | 15 | 15 |
| y7 (1+) | 14 | 5.3 | 3.1 | 16.3 | 6.5 | 4.3 | 21.5 | 8.4 | 5.3 | 15 | 15 | 15 |
| y8 (1+) | 13 | 7.6 | 8.4 | 13.1 | 7.2 | 10.3 | 18.5 | 10.5 | 13.3 | 15 | 15 | 15 |
| sum | 6 | 3.2 | 2.6 | 5.5 | 3.5 | 2.5 | 8.1 | 4.7 | 3.6 | 15 | 15 | 15 |