MAPK9, CPTAC-893 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-3146: View additional EHAIEEWK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MSDSKCDSQF | YSVQVADSTF | TVLKRYQQLK | PIGSGAQGIV | CAAFDTVLGI |
| 60 | 70 | 80 | 90 | 100 |
| NVAVKKLSRP | FQNQTHAKRA | YRELVLLKCV | NHKNIISLLN | VFTPQKTLEE |
| 110 | 120 | 130 | 140 | 150 |
| FQDVYLVMEL | MDANLCQVIH | MELDHERMSY | LLYQMLCGIK | HLHSAGIIHR |
| 160 | 170 | 180 | 190 | 200 |
| DLKPSNIVVK | SDCTLKILDF | GLARTACTNF | MMTPYVVTRY | YRAPEVILGM |
| 210 | 220 | 230 | 240 | 250 |
| GYKENVDIWS | VGCIMGELVK | GCVIFQGTDH | IDQWNKVIEQ | LGTPSAEFMK |
| 260 | 270 | 280 | 290 | 300 |
| KLQPTVRNYV | ENRPKYPGIK | FEELFPDWIF | PSESERDKIK | TSQARDLLSK |
| 310 | 320 | 330 | 340 | 350 |
| MLVIDPDKRI | SVDEALRHPY | ITVWYDPAEA | EAPPPQIYDA | QLEEREHAIE |
| 360 | 370 | 380 | 390 | 400 |
| EWKELIYKEV | MDWEERSKNG | VVKDQPSDAA | VSSNATPSQS | SSINDISSMS |
| 410 | 420 | 424 | ||
| TEQTLASDTD | SSLDASTGPL | EGCR |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-3146 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK9
Peptide Sequence
EHAIEEWK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
346
Peptide End
353
CPTAC ID
CPTAC-3146
Peptide Molecular Mass
1,040.4927
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 7.6 | 6.6 | 11.6 | 6.2 | 7.1 | 11.5 | 9.8 | 9.7 | 16.3 | 15 | 15 | 15 |
| y6 (1+) | 3.8 | 7.1 | 8.2 | 5.7 | 7.7 | 10.2 | 6.9 | 10.5 | 13.1 | 15 | 15 | 15 |
| y4 (1+) | 11.4 | 7.8 | 12 | 12.9 | 9.9 | 13.8 | 17.2 | 12.6 | 18.3 | 15 | 15 | 15 |
| sum | 3.2 | 5.7 | 8.4 | 4 | 6.2 | 9.5 | 5.1 | 8.4 | 12.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-893 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK9
Peptide Sequence
FEELFPDWIFPSESER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
271
Peptide End
286
CPTAC ID
CPTAC-893
Peptide Molecular Mass
2,026.9313
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 74.4 | 40 | 19.9 | 69.6 | 48.3 | 27.7 | 101.9 | 62.7 | 34.1 | 10 | 15 | 15 |
| y4 (1+) | 105.6 | 42 | 27.9 | 113.5 | 45.1 | 42.3 | 155 | 61.6 | 50.7 | 11 | 15 | 15 |
| y5 (1+) | 24.8 | 15.2 | 21.7 | 48.3 | 27.2 | 22.9 | 54.3 | 31.2 | 31.5 | 10 | 15 | 15 |
| y6 (1+) | 37.8 | 17 | 8.8 | 102.5 | 21.1 | 10.5 | 109.2 | 27.1 | 13.7 | 13 | 15 | 15 |
| y7 (1+) | 35.9 | 15.8 | 12.3 | 74.9 | 15.7 | 14 | 83.1 | 22.3 | 18.6 | 12 | 15 | 15 |
| y8 (1+) | 75 | 45.4 | 37.8 | 87.4 | 47.4 | 42.5 | 115.2 | 65.6 | 56.9 | 10 | 12 | 15 |
| y9 (1+) | 77.6 | 58.4 | 42.6 | 95.2 | 66.2 | 33.1 | 122.8 | 88.3 | 53.9 | 8 | 15 | 15 |
| y10 (1+) | 110.9 | 61.9 | 33.8 | 145.6 | 59.1 | 49.8 | 183 | 85.6 | 60.2 | 9 | 11 | 13 |
| y11 (1+) | 60.7 | 62.4 | 43.8 | 157.3 | 141.6 | 48.4 | 168.6 | 154.7 | 65.3 | 6 | 11 | 14 |
| b11 (1+) | NA | NA | NA | NA | NA | NA | NA | NA | NA | 0 | 1 | 1 |
| b10 (1+) | NA | 94.3 | NA | NA | 0.2 | NA | NA | 94.3 | NA | 3 | 4 | 3 |
| b9 (1+) | 45.7 | 44.2 | 77.3 | 173.2 | 38.5 | 86.4 | 179.1 | 58.6 | 115.9 | 5 | 7 | 13 |
| b8 (1+) | 44.7 | 58.8 | 29.8 | 81 | 54.3 | 27.3 | 92.5 | 80 | 40.4 | 9 | 15 | 13 |
| b7 (1+) | 82.8 | 51.5 | 75.3 | 113.7 | 51.4 | 79.8 | 140.7 | 72.8 | 109.7 | 11 | 12 | 14 |
| b6 (1+) | 141.3 | 72.8 | 73.8 | 36.8 | 77.3 | 71.4 | 146 | 106.2 | 102.7 | 9 | 9 | 12 |
| b5 (1+) | 77.7 | 60.1 | 56 | 62.5 | 67.6 | 79.6 | 99.7 | 90.5 | 97.3 | 10 | 14 | 15 |
| b4 (1+) | 64.9 | 38.2 | 23.8 | 138.6 | 38.6 | 30 | 153 | 54.3 | 38.3 | 11 | 15 | 15 |
| b3 (1+) | 73.9 | 54.7 | 17.4 | 71.7 | 73.7 | 20.6 | 103 | 91.8 | 27 | 13 | 15 | 15 |
| sum | 50 | 6.4 | 4 | 88.4 | 11 | 5.2 | 101.6 | 12.7 | 6.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-894 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK9
Peptide Sequence
VIEQLGTPSAEFMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
237
Peptide End
250
CPTAC ID
CPTAC-894
Peptide Molecular Mass
1,548.7858
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 5.7 | 6.4 | 7.3 | 8.4 | 6.2 | 6.7 | 10.2 | 8.9 | 9.9 | 15 | 15 | 15 |
| y4 (1+) | 18.8 | 10.2 | 6.4 | 17.9 | 9.1 | 5.8 | 26 | 13.7 | 8.6 | 15 | 15 | 15 |
| y5 (1+) | 16.9 | 11.6 | 3.5 | 15.3 | 11.4 | 5.5 | 22.8 | 16.3 | 6.5 | 15 | 15 | 15 |
| y6 (1+) | 15.4 | 8.4 | 6.2 | 14 | 7.6 | 6.4 | 20.8 | 11.3 | 8.9 | 15 | 15 | 15 |
| y7 (1+) | 5.4 | 3.3 | 1.9 | 4.7 | 4.7 | 2.2 | 7.2 | 5.7 | 2.9 | 15 | 15 | 15 |
| y8 (1+) | 20.5 | 8.2 | 4.2 | 19.9 | 6.9 | 5.3 | 28.6 | 10.7 | 6.8 | 15 | 15 | 15 |
| y9 (1+) | 5.2 | 3.5 | 2.2 | 8.7 | 4 | 2.2 | 10.1 | 5.3 | 3.1 | 15 | 15 | 15 |
| y10 (1+) | 12 | 10.4 | 4.5 | 12.3 | 11.2 | 4.8 | 17.2 | 15.3 | 6.6 | 15 | 15 | 15 |
| b4 (1+) | 21.7 | 10.9 | 8.6 | 21.5 | 9.6 | 12.1 | 30.5 | 14.5 | 14.8 | 15 | 15 | 15 |
| b3 (1+) | 26.6 | 4.7 | 4.5 | 25.1 | 6.5 | 4.6 | 36.6 | 8 | 6.4 | 15 | 15 | 15 |
| sum | 3.6 | 2.6 | 1.4 | 3.7 | 2.4 | 1.8 | 5.2 | 3.5 | 2.3 | 15 | 15 | 15 |