RIPK3, CPTAC-903 - CPTAC Assay Portal (original) (raw)
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Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| RIPK3 | RIP3 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 518 | 56887 |
| Protein Name |
|---|
| Receptor-interacting serine/threonine-protein kinase 3 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MSCVKLWPSG | APAPLVSIEE | LENQELVGKG | GFGTVFRAQH | RKWGYDVAVK |
| 60 | 70 | 80 | 90 | 100 |
| IVNSKAISRE | VKAMASLDNE | FVLRLEGVIE | KVNWDQDPKP | ALVTKFMENG |
| 110 | 120 | 130 | 140 | 150 |
| SLSGLLQSQC | PRPWPLLCRL | LKEVVLGMFY | LHDQNPVLLH | RDLKPSNVLL |
| 160 | 170 | 180 | 190 | 200 |
| DPELHVKLAD | FGLSTFQGGS | QSGTGSGEPG | GTLGYLAPEL | FVNVNRKAST |
| 210 | 220 | 230 | 240 | 250 |
| ASDVYSFGIL | MWAVLAGREV | ELPTEPSLVY | EAVCNRQNRP | SLAELPQAGP |
| 260 | 270 | 280 | 290 | 300 |
| ETPGLEGLKE | LMQLCWSSEP | KDRPSFQECL | PKTDEVFQMV | ENNMNAAVST |
| 310 | 320 | 330 | 340 | 350 |
| VKDFLSQLRS | SNRRFSIPES | GQGGTEMDGF | RRTIENQHSR | NDVMVSEWLN |
| 360 | 370 | 380 | 390 | 400 |
| KLNLEEPPSS | VPKKCPSLTK | RSRAQEEQVP | QAWTAGTSSD | SMAQPPQTPE |
| 410 | 420 | 430 | 440 | 450 |
| TSTFRNQMPS | PTSTGTPSPG | PRGNQGAERQ | GMNWSCRTPE | PNPVTGRPLV |
| 460 | 470 | 480 | 490 | 500 |
| NIYNCSGVQV | GDNNYLTMQQ | TTALPTWGLA | PSGKGRGLQH | PPPVGSQEGP |
| 510 | 518 | |||
| KDPEAWSRPQ | GWYNHSGK |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-903 Collapse assay details
Data source: Panorama
Official Gene Symbol
RIPK3
Peptide Sequence
DLKPSNVLLDPELHVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
142
Peptide End
157
CPTAC ID
CPTAC-903
Peptide Molecular Mass
1,816.0095
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 11.7 | 4 | 6.7 | 9.5 | 4 | 6.4 | 15.1 | 5.7 | 9.3 | 15 | 15 | 15 |
| y6 (1+) | 9.9 | 5.3 | 2.7 | 9.9 | 5 | 3.2 | 14 | 7.3 | 4.2 | 15 | 15 | 15 |
| b6 (1+) | 10.3 | 8.7 | 3.7 | 12.6 | 11.4 | 4.7 | 16.3 | 14.3 | 6 | 15 | 15 | 15 |
| sum | 6.7 | 4.5 | 2.3 | 7.2 | 4.3 | 2.4 | 9.8 | 6.2 | 3.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-904 Collapse assay details
Data source: Panorama
Official Gene Symbol
RIPK3
Peptide Sequence
VNWDQDPKPALVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
82
Peptide End
95
CPTAC ID
CPTAC-904
Peptide Molecular Mass
1,609.8464
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 12.7 | 9.8 | 6.9 | 18 | 10.9 | 10.2 | 22 | 14.7 | 12.3 | 15 | 15 | 15 |
| y5 (1+) | 29.8 | 9.3 | 8.2 | 27.1 | 11.9 | 8.5 | 40.3 | 15.1 | 11.8 | 15 | 15 | 15 |
| y6 (1+) | 4.1 | 4 | 3 | 6.7 | 4.2 | 5.3 | 7.9 | 5.8 | 6.1 | 15 | 15 | 15 |
| y7 (1+) | 21.7 | 13.4 | 11 | 22.6 | 10.3 | 11.3 | 31.3 | 16.9 | 15.8 | 15 | 15 | 15 |
| y8 (1+) | 10 | 4.9 | 5.1 | 11.5 | 6.3 | 6.6 | 15.2 | 8 | 8.3 | 15 | 15 | 15 |
| sum | 3.6 | 3.6 | 2.4 | 6.3 | 3.6 | 4.3 | 7.3 | 5.1 | 4.9 | 15 | 15 | 15 |