SRC, CPTAC-905 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

CPTAC-3071: View additional LDSGGFYITSR data

10 20 30 40 50
MGSNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP
60 70 80 90 100
SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE
110 120 130 140 150
TDLSFKKGER LQIVNNTEGD WWLAHSLSTG QTGYIPSNYV APSDSIQAEE
160 170 180 190 200
WYFGKITRRE SERLLLNAEN PRGTFLVRES ETTKGAYCLS VSDFDNAKGL
210 220 230 240 250
NVKHYKIRKL DSGGFYITSR TQFNSLQQLV AYYSKHADGL CHRLTTVCPT
260 270 280 290 300
SKPQTQGLAK DAWEIPRESL RLEVKLGQGC FGEVWMGTWN GTTRVAIKTL
310 320 330 340 350
KPGTMSPEAF LQEAQVMKKL RHEKLVQLYA VVSEEPIYIV TEYMSKGSLL
360 370 380 390 400
DFLKGETGKY LRLPQLVDMA AQIASGMAYV ERMNYVHRDL RAANILVGEN
410 420 430 440 450
LVCKVADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS
460 470 480 490 500
FGILLTELTT KGRVPYPGMV NREVLDQVER GYRMPCPPEC PESLHDLMCQ
510 520 530 536
CWRKEPEERP TFEYLQAFLE DYFTSTEPQY QPGENL

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-3072 Collapse assay details

Data source: Panorama

Official Gene Symbol

SRC

Peptide Modified Sequence

GAYC[+57.0]LSVSDFDNAK

Modification Type

Carbamidomethyl (C)

Protein - Site of Modification

189

Peptide - Site of Modification

4

Peptide Start

185

Peptide End

198

CPTAC ID

CPTAC-3072

Peptide Molecular Mass

1,545.6770

Species

Homo Sapiens

Assay Type

Direct PRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher, Q-Exactive

Internal Standard

25 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 µm x 50 cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y9 (1+) | 4.7 | 6 | 3.5 | 6.6 | 5.8 | 3.6 | 8.1 | 8.3 | 5 | 15 | 15 | 15 | | y8 (1+) | 5.5 | 5.4 | 3.6 | 5.4 | 5.6 | 3.5 | 7.7 | 7.8 | 5 | 15 | 15 | 15 | | y7 (1+) | 4.4 | 6.7 | 3 | 5.5 | 6.1 | 3.7 | 7 | 9.1 | 4.8 | 15 | 15 | 15 | | sum | 4 | 6 | 3.2 | 5.4 | 5.8 | 3.5 | 6.7 | 8.3 | 4.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-3071 Collapse assay details

Data source: Panorama

Official Gene Symbol

SRC

Peptide Sequence

LDSGGFYITSR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

210

Peptide End

220

CPTAC ID

CPTAC-3071

Peptide Molecular Mass

1,214.5932

Species

Homo Sapiens

Assay Type

Direct PRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher, Q-Exactive

Internal Standard

25 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 µm x 50 cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 5.1 | 9.4 | 5.9 | 6.2 | 11 | 6.3 | 8 | 14.5 | 8.6 | 15 | 15 | 15 | | y5 (1+) | 5.2 | 6.4 | 4.6 | 7.2 | 8.1 | 6 | 8.9 | 10.3 | 7.6 | 15 | 15 | 15 | | y9 (1+) | 6.2 | 7.6 | 4.3 | 8 | 9.4 | 6.5 | 10.1 | 12.1 | 7.8 | 15 | 15 | 15 | | sum | 5.4 | 7.5 | 4.5 | 7 | 9.3 | 6 | 8.8 | 11.9 | 7.5 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-905 Collapse assay details

Data source: Panorama

Official Gene Symbol

SRC

Peptide Modified Sequence

LIEDNEY[+80.0]TAR

Modification Type

Phospho (Y)

Protein - Site of Modification

419

Peptide - Site of Modification

7

Peptide Start

388

Peptide End

397

CPTAC ID

CPTAC-905

Peptide Molecular Mass

1,302.5493

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Pooled patient derived xenograft breast tumor digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TripleTOF 5600+ (SCIEX)

Internal Standard

Peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

2DPlus nano-LC (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

200 µm x 15 cm

Flow Rate

800 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y3 (1+) | 25.3 | 11.9 | 11.2 | 31.4 | 14.5 | 15.2 | 40.3 | 18.8 | 18.9 | 15 | 15 | 15 | | y4 (1+) | 14.3 | 11.6 | 8.5 | 14.5 | 14.4 | 7.6 | 20.4 | 18.5 | 11.4 | 15 | 15 | 15 | | y5 (1+) | 21.1 | 10.3 | 8.4 | 23.6 | 11.5 | 10.9 | 31.7 | 15.4 | 13.8 | 15 | 15 | 15 | | y6 (1+) | 6.5 | 6.9 | 7.7 | 11.6 | 9 | 9.6 | 13.3 | 11.3 | 12.3 | 15 | 15 | 15 | | y7 (1+) | 14.4 | 8.1 | 7.4 | 12.6 | 8.3 | 9.9 | 19.1 | 11.6 | 12.4 | 15 | 15 | 15 | | y8 (1+) | 12.9 | 7.1 | 7.1 | 14.9 | 8.6 | 8 | 19.7 | 11.2 | 10.7 | 15 | 15 | 15 | | sum | 3.4 | 5.9 | 4.6 | 6 | 7.2 | 6 | 6.9 | 9.3 | 7.6 | 15 | 15 | 15 |

Additional Resources and Comments