CD3EAP, CPTAC-920 - CPTAC Assay Portal (original) (raw)
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Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| CD3EAP | ASE1, CAST, PAF49 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 510 | 54986 |
| Protein Name |
|---|
| DNA-directed RNA polymerase I subunit RPA34 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MEEPQAGDAA | RFSCPPNFTA | KPPASESPRF | SLEALTGPDT | ELWLIQAPAD |
| 60 | 70 | 80 | 90 | 100 |
| FAPECFNGRH | VPLSGSQIVK | GKLAGKRHRY | RVLSSCPQAG | EATLLAPSTE |
| 110 | 120 | 130 | 140 | 150 |
| AGGGLTCASA | PQGTLRILEG | PQQSLSGSPL | QPIPASPPPQ | IPPGLRPRFC |
| 160 | 170 | 180 | 190 | 200 |
| AFGGNPPVTG | PRSALAPNLL | TSGKKKKEMQ | VTEAPVTQEA | VNGHGALEVD |
| 210 | 220 | 230 | 240 | 250 |
| MALGSPEMDV | RKKKKKKNQQ | LKEPEAAGPV | GTEPTVETLE | PLGVLFPSTT |
| 260 | 270 | 280 | 290 | 300 |
| KKRKKPKGKE | TFEPEDKTVK | QEQINTEPLE | DTVLSPTKKR | KRQKGTEGME |
| 310 | 320 | 330 | 340 | 350 |
| PEEGVTVESQ | PQVKVEPLEE | AIPLPPTKKR | KKEKGQMAMM | EPGTEAMEPV |
| 360 | 370 | 380 | 390 | 400 |
| EPEMKPLESP | GGTMAPQQPE | GAKPQAQAAL | AAPKKKTKKE | KQQDATVEPE |
| 410 | 420 | 430 | 440 | 450 |
| TEVVGPELPD | DLEPQAAPTS | TKKKKKKKER | GHTVTEPIQP | LEPELPGEGQ |
| 460 | 470 | 480 | 490 | 500 |
| PEARATPGST | KKRKKQSQES | RMPETVPQEE | MPGPPLNSES | GEEAPTGRDK |
| 510 | ||||
| KRKQQQQQPV |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-920 Collapse assay details
Data source: Panorama
Official Gene Symbol
CD3EAP
Peptide Modified Sequence
FSC[+57.0]PPNFTAKPPASES[+80.0]PR
Modification Type
Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
14, 27
Peptide - Site of Modification
3, 16
Peptide Start
12
Peptide End
29
CPTAC ID
CPTAC-920
Peptide Molecular Mass
2,068.9078
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y14 (2+) | 37 | 25.8 | 31.4 | 43.5 | 26.2 | 48 | 57.1 | 36.8 | 57.4 | 12 | 24 | 15 |
| y15 (2+) | 42.7 | 25.6 | 24.1 | 32.9 | 31.9 | 32.8 | 53.9 | 40.9 | 40.7 | 12 | 24 | 15 |
| y8 (1+) | 45.9 | 36.5 | 65.3 | 26.7 | 42.1 | 50.8 | 53.1 | 55.7 | 82.7 | 12 | 24 | 15 |
| sum | 35.3 | 21.9 | 24 | 25.8 | 25.5 | 25.8 | 43.7 | 33.6 | 35.2 | 12 | 24 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-921 Collapse assay details
Data source: Panorama
Official Gene Symbol
CD3EAP
Peptide Modified Sequence
QEQINTEPLEDTVLS[+80.0]PTK
Modification Type
Phospho (ST)
Protein - Site of Modification
285
Peptide - Site of Modification
15
Peptide Start
271
Peptide End
288
CPTAC ID
CPTAC-921
Peptide Molecular Mass
2,120.9879
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b7 (1+) | 23.3 | 11.2 | 22.3 | 39.2 | 19.7 | 24 | 45.6 | 22.7 | 32.8 | 12 | 20 | 13 |
| y11 (2+) | 22.9 | 23.5 | 16.9 | 24.8 | 33.6 | 28.7 | 33.8 | 41 | 33.3 | 12 | 20 | 13 |
| y4 (1+) | 39 | 24.9 | 36.9 | 35.5 | 26.5 | 45.7 | 52.7 | 36.4 | 58.7 | 12 | 20 | 13 |
| sum | 15.6 | 13.7 | 16.8 | 20.7 | 21.6 | 27.2 | 25.9 | 25.6 | 32 | 12 | 20 | 13 |