NAP1L1, CPTAC-97 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MADIDNKEQS | ELDQDLDDVE | EVEEEETGEE | TKLKARQLTV | QMMQNPQILA |
| 60 | 70 | 80 | 90 | 100 |
| ALQERLDGLV | ETPTGYIESL | PRVVKRRVNA | LKNLQVKCAQ | IEAKFYEEVH |
| 110 | 120 | 130 | 140 | 150 |
| DLERKYAVLY | QPLFDKRFEI | INAIYEPTEE | ECEWKPDEED | EISEELKEKA |
| 160 | 170 | 180 | 190 | 200 |
| KIEDEKKDEE | KEDPKGIPEF | WLTVFKNVDL | LSDMVQEHDE | PILKHLKDIK |
| 210 | 220 | 230 | 240 | 250 |
| VKFSDAGQPM | SFVLEFHFEP | NEYFTNEVLT | KTYRMRSEPD | DSDPFSFDGP |
| 260 | 270 | 280 | 290 | 300 |
| EIMGCTGCQI | DWKKGKNVTL | KTIKKKQKHK | GRGTVRTVTK | TVSNDSFFNF |
| 310 | 320 | 330 | 340 | 350 |
| FAPPEVPESG | DLDDDAEAIL | AADFEIGHFL | RERIIPRSVL | YFTGEAIEDD |
| 360 | 370 | 380 | 390 | 391 |
| DDDYDEEGEE | ADEEGEEEGD | EENDPDYDPK | KDQNPAECKQ | Q |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-98 Collapse assay details
Data source: Panorama
Official Gene Symbol
NAP1L1
Peptide Sequence
GIPEFWLTVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
166
Peptide End
176
CPTAC ID
CPTAC-98
Peptide Molecular Mass
1,335.7227
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 14.5 | 10.1 | 6 | 26.6 | 22.7 | 21 | 30.3 | 24.8 | 21.8 | 15 | 25 | 15 |
| y7 (1+) | 8 | 11.3 | 6.1 | 29.6 | 23.5 | 21.8 | 30.7 | 26.1 | 22.6 | 15 | 25 | 15 |
| y6 (1+) | 11.5 | 11.6 | 7.2 | 20 | 20.3 | 20.9 | 23.1 | 23.4 | 22.1 | 15 | 25 | 15 |
| sum | 8.2 | 10.9 | 6.3 | 21.9 | 22.1 | 21.3 | 23.4 | 24.6 | 22.2 | 15 | 25 | 15 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|
Additional Resources and Comments
Assay Details for CPTAC-97 Collapse assay details
Data source: Panorama
Official Gene Symbol
NAP1L1
Peptide Sequence
LDGLVETPTGYIESLPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
56
Peptide End
72
CPTAC ID
CPTAC-97
Peptide Molecular Mass
1,858.9676
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 15.3 | 9.3 | 4.3 | 28.4 | 12.8 | 12.1 | 32.3 | 15.8 | 12.8 | 15 | 25 | 15 |
| y10 (1+) | 12.3 | 6.5 | 4.9 | 21.9 | 11.2 | 13.5 | 25.1 | 12.9 | 14.4 | 15 | 25 | 15 |
| y4 (1+) | 19 | 11.6 | 9.8 | 30 | 15 | 14.5 | 35.5 | 19 | 17.5 | 15 | 25 | 15 |
| sum | 10.9 | 6.6 | 4.5 | 22.3 | 11.4 | 11.9 | 24.8 | 13.2 | 12.7 | 15 | 25 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||||
|---|---|---|---|---|---|---|---|---|
| fragment_ion | HCC1187 | HCC1419 | HCC1954 | HCC70 | MCF12A | MDAMB175VII | ZR751 | ZR7530 |
| y11 (1+) | -0.06 | -0.1 | -0.14 | -0.41 | -0.06 | -0.09 | -0.07 | -0.01 |
| y10 (1+) | -0.06 | -0.1 | -0.15 | -0.37 | -0.05 | -0.09 | -0.08 | -0.03 |
| y4 (1+) | -0.09 | -0.1 | -0.23 | -0.54 | -0.06 | -0.17 | -0.11 | -0.05 |
| sum | -0.06 | -0.1 | -0.15 | -0.39 | -0.05 | -0.1 | -0.08 | -0.03 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y4 (1+) | 7.2 | 9.5 | 11.4 | NA | 7.9 |
| y10 (1+) | 3.2 | 4.1 | 1.8 | NA | 8.5 |
| y11 (1+) | 6.1 | 3.6 | 8 | NA | 3.3 |
| sum | 3.9 | 3.5 | 3.6 | NA | 6.6 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y4 (1+) | 9.1 | 8.4 |
| y10 (1+) | 4.3 | 4.8 |
| y11 (1+) | 4.9 | 5.9 |
| sum | 4.2 | 4.7 |
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y10 (1+) | 6.7 | 10.8 | 12.7 |
| y11 (1+) | 9.1 | 12.1 | 15.1 |
| y4 (1+) | 14.2 | 16.9 | 22.1 |
| sum | 6.7 | 10.9 | 12.8 |