NFATC1, CPTAC-983 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| NFATC1 | NFAT2, NFATC |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 943 | 101243 |
| Protein Name |
|---|
| Nuclear factor of activated T-cells, cytoplasmic 1 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MPSTSFPVPS | KFPLGPAAAV | FGRGETLGPA | PRAGGTMKSA | EEEHYGYASS |
| 60 | 70 | 80 | 90 | 100 |
| NVSPALPLPT | AHSTLPAPCH | NLQTSTPGII | PPADHPSGYG | AALDGGPAGY |
| 110 | 120 | 130 | 140 | 150 |
| FLSSGHTRPD | GAPALESPRI | EITSCLGLYH | NNNQFFHDVE | VEDVLPSSKR |
| 160 | 170 | 180 | 190 | 200 |
| SPSTATLSLP | SLEAYRDPSC | LSPASSLSSR | SCNSEASSYE | SNYSYPYASP |
| 210 | 220 | 230 | 240 | 250 |
| QTSPWQSPCV | SPKTTDPEEG | FPRGLGACTL | LGSPRHSPST | SPRASVTEES |
| 260 | 270 | 280 | 290 | 300 |
| WLGARSSRPA | SPCNKRKYSL | NGRQPPYSPH | HSPTPSPHGS | PRVSVTDDSW |
| 310 | 320 | 330 | 340 | 350 |
| LGNTTQYTSS | AIVAAINALT | TDSSLDLGDG | VPVKSRKTTL | EQPPSVALKV |
| 360 | 370 | 380 | 390 | 400 |
| EPVGEDLGSP | PPPADFAPED | YSSFQHIRKG | GFCDQYLAVP | QHPYQWAKPK |
| 410 | 420 | 430 | 440 | 450 |
| PLSPTSYMSP | TLPALDWQLP | SHSGPYELRI | EVQPKSHHRA | HYETEGSRGA |
| 460 | 470 | 480 | 490 | 500 |
| VKASAGGHPI | VQLHGYLENE | PLMLQLFIGT | ADDRLLRPHA | FYQVHRITGK |
| 510 | 520 | 530 | 540 | 550 |
| TVSTTSHEAI | LSNTKVLEIP | LLPENSMRAV | IDCAGILKLR | NSDIELRKGE |
| 560 | 570 | 580 | 590 | 600 |
| TDIGRKNTRV | RLVFRVHVPQ | PSGRTLSLQV | ASNPIECSQR | SAQELPLVEK |
| 610 | 620 | 630 | 640 | 650 |
| QSTDSYPVVG | GKKMVLSGHN | FLQDSKVIFV | EKAPDGHHVW | EMEAKTDRDL |
| 660 | 670 | 680 | 690 | 700 |
| CKPNSLVVEI | PPFRNQRITS | PVHVSFYVCN | GKRKRSQYQR | FTYLPANVPI |
| 710 | 720 | 730 | 740 | 750 |
| IKTEPTDDYE | PAPTCGPVSQ | GLSPLPRPYY | SQQLAMPPDP | SSCLVAGFPP |
| 760 | 770 | 780 | 790 | 800 |
| CPQRSTLMPA | APGVSPKLHD | LSPAAYTKGV | ASPGHCHLGL | PQPAGEAPAV |
| 810 | 820 | 830 | 840 | 850 |
| QDVPRPVATH | PGSPGQPPPA | LLPQQVSAPP | SSSCPPGLEH | SLCPSSPSPP |
| 860 | 870 | 880 | 890 | 900 |
| LPPATQEPTC | LQPCSPACPP | ATGRPQHLPS | TVRRDESPTA | GPRLLPEVHE |
| 910 | 920 | 930 | 940 | 943 |
| DGSPNLAPIP | VTVKREPEEL | DQLYLDDVNE | IIRNDLSSTS | THS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
loading
Assay Details for CPTAC-983 Collapse assay details
Data source: Panorama
Official Gene Symbol
NFATC1
Peptide Modified Sequence
GLGAC[+57.0]TLLGS[+80.0]PR
Modification Type
Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
228, 233
Peptide - Site of Modification
5, 10
Peptide Start
224
Peptide End
235
CPTAC ID
CPTAC-983
Peptide Molecular Mass
1,280.5948
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b8 (1+) | 33.8 | 23.8 | 42.4 | 30.3 | 23.3 | 43.5 | 45.4 | 33.3 | 60.7 | 15 | 24 | 15 |
| y4 (1+) | 29.9 | 11.3 | 22.7 | 19.6 | 10.2 | 20.6 | 35.8 | 15.2 | 30.7 | 15 | 24 | 15 |
| y5 (1+) | 34.9 | 23.5 | 21 | 28.5 | 36.2 | 26.8 | 45.1 | 43.2 | 34 | 15 | 24 | 15 |
| sum | 29.3 | 13.8 | 11 | 17.9 | 18.1 | 14 | 34.3 | 22.8 | 17.8 | 15 | 24 | 15 |