ADIPOQ, CPTAC-1057 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
non-CPTAC-3336: View additional AVLFTYDQYQEK data
non-CPTAC-1057: View additional GFPGIQGR data
non-CPTAC-3294: View additional SAFSVGLETR data
non-CPTAC-3335: View additional VTVPNVPIR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MLLLGAVLLL | LALPGHDQET | TTQGPGVLLP | LPKGACTGWM | AGIPGHPGHN |
| 60 | 70 | 80 | 90 | 100 |
| GAPGRDGRDG | TPGEKGEKGD | PGLIGPKGDI | GETGVPGAEG | PRGFPGIQGR |
| 110 | 120 | 130 | 140 | 150 |
| KGEPGEGAYV | YRSAFSVGLE | TYVTIPNMPI | RFTKIFYNQQ | NHYDGSTGKF |
| 160 | 170 | 180 | 190 | 200 |
| HCNIPGLYYF | AYHITVYMKD | VKVSLFKKDK | AMLFTYDQYQ | ENNVDQASGS |
| 210 | 220 | 230 | 240 | 244 |
| VLLHLEVGDQ | VWLQVYGEGE | RNGLYADNDN | DSTFTGFLLY | HDTN |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
loading
Assay Details for non-CPTAC-3336 Collapse assay details
Data source: Panorama
Official Gene Symbol
Adipoq
Peptide Sequence
AVLFTYDQYQEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
184
Peptide End
195
CPTAC ID
non-CPTAC-3336
Peptide Molecular Mass
1,503.7246
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (2+) | 8.7 | 6.4 | 5 | 13.5 | 8.1 | 6.3 | 16.1 | 10.3 | 8 | 15 | 15 | 15 |
| y2 (1+) | 4.1 | 5.7 | 5.4 | 14.9 | 6.6 | 6.8 | 15.5 | 8.7 | 8.7 | 15 | 15 | 15 |
| y6 (1+) | 4.7 | 3.6 | 4.1 | 16.3 | 6.3 | 3.5 | 17 | 7.3 | 5.4 | 15 | 15 | 15 |
| y7 (1+) | 5.5 | 6.4 | 4 | 13.6 | 7.5 | 5.8 | 14.7 | 9.9 | 7 | 15 | 15 | 15 |
| y8 (1+) | 6.4 | 4.9 | 4.6 | 14.4 | 7 | 4.1 | 15.8 | 8.5 | 6.2 | 15 | 15 | 15 |
| sum | 2.7 | 0.7 | 2.8 | 14.4 | 5.6 | 3.3 | 14.7 | 5.6 | 4.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-1057 Collapse assay details
Data source: Panorama
Official Gene Symbol
ADIPOQ
Peptide Sequence
GFPGIQGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
93
Peptide End
100
CPTAC ID
non-CPTAC-1057
Peptide Molecular Mass
830.4399
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 14.3 | 5.7 | 7.7 | 12.3 | 7.8 | 6.9 | 18.9 | 9.7 | 10.3 | 13 | 15 | 15 |
| y6 (2+) | 6.3 | 4.9 | 3.3 | 7.4 | 5.2 | 3.3 | 9.7 | 7.1 | 4.7 | 15 | 15 | 15 |
| y3 (1+) | 50 | 11.4 | 4.6 | 43.2 | 14 | 5.3 | 66.1 | 18.1 | 7 | 13 | 15 | 15 |
| y5 (1+) | 18.1 | 7.7 | 5.6 | 18.9 | 8.1 | 5.4 | 26.2 | 11.2 | 7.8 | 15 | 15 | 15 |
| y6 (1+) | 12 | 6 | 4.3 | 19.3 | 7.5 | 5.5 | 22.7 | 9.6 | 7 | 14 | 15 | 15 |
| sum | 6.1 | 4.6 | 2.9 | 6.8 | 4.9 | 3 | 9.1 | 6.7 | 4.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3294 Collapse assay details
Data source: Panorama
Official Gene Symbol
Adipoq
Peptide Sequence
SAFSVGLETR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
116
Peptide End
125
CPTAC ID
non-CPTAC-3294
Peptide Molecular Mass
1,065.5455
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 7.9 | 6 | 5 | 12.2 | 10.2 | 7.1 | 14.5 | 11.8 | 8.7 | 15 | 15 | 15 |
| y2 (1+) | 5.5 | 5.1 | 5.2 | 27.5 | 15.4 | 21.6 | 28 | 16.2 | 22.2 | 15 | 15 | 15 |
| y6 (1+) | 5.7 | 6.6 | 4.8 | 14.2 | 10.5 | 5.3 | 15.3 | 12.4 | 7.2 | 15 | 15 | 15 |
| y8 (2+) | 6.3 | 5.6 | 6.2 | 17.6 | 8 | 7.6 | 18.7 | 9.8 | 9.8 | 15 | 15 | 15 |
| y5 (1+) | 3.7 | 4.5 | 4.9 | 17.1 | 4.8 | 7.7 | 17.5 | 6.6 | 9.1 | 15 | 15 | 15 |
| sum | 2 | 2.5 | 3.6 | 14.5 | 4.9 | 7.2 | 14.6 | 5.5 | 8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3335 Collapse assay details
Data source: Panorama
Official Gene Symbol
Adipoq
Peptide Sequence
VTVPNVPIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
126
Peptide End
134
CPTAC ID
non-CPTAC-3335
Peptide Molecular Mass
993.5971
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 3.7 | 5.2 | 3.1 | 16.3 | 6.9 | 4.8 | 16.7 | 8.6 | 5.7 | 15 | 15 | 15 |
| y7 (2+) | 4 | 3.5 | 5.6 | 14.1 | 5.3 | 5.9 | 14.7 | 6.4 | 8.1 | 15 | 15 | 15 |
| y3 (1+) | 6 | 6.5 | 6.9 | 16.8 | 8.4 | 6.9 | 17.8 | 10.6 | 9.8 | 15 | 15 | 15 |
| y6 (1+) | 4.2 | 5.4 | 2.7 | 15.6 | 9.4 | 5.2 | 16.2 | 10.8 | 5.9 | 15 | 15 | 15 |
| y7 (1+) | 5.5 | 4.2 | 4.8 | 10.7 | 7.7 | 6.2 | 12 | 8.8 | 7.8 | 15 | 15 | 15 |
| sum | 2.9 | 3.7 | 2.2 | 14.9 | 7.8 | 3.6 | 15.2 | 8.6 | 4.2 | 15 | 15 | 15 |