APOC1, CPTAC-1085 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
non-CPTAC-2623: View additional EWFSETFQK data
non-CPTAC-1085: View additional TPDVSSALDK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MRLFLSLPVL | VVVLSIVLEG | PAPAQGTPDV | SSALDKLKEF | GNTLEDKARE |
| 60 | 70 | 80 | 83 | |
| LISRIKQSEL | SAKMREWFSE | TFQKVKEKLK | IDS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
loading
Assay Details for non-CPTAC-2623 Collapse assay details
Data source: Panorama
Official Gene Symbol
APOC1
Peptide Sequence
EWFSETFQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
66
Peptide End
74
CPTAC ID
non-CPTAC-2623
Peptide Molecular Mass
1,200.5451
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 12.2 | 5.1 | 7.9 | 15.8 | 6.4 | 8.3 | 20 | 8.2 | 11.5 | 15 | 15 | 15 |
| y6 (1+) | 12.1 | 6.8 | 6.6 | 16.6 | 8 | 6.8 | 20.5 | 10.5 | 9.5 | 15 | 15 | 15 |
| y7 (2+) | 25.2 | 6.1 | 8.5 | 30.2 | 8.5 | 8.2 | 39.3 | 10.5 | 11.8 | 15 | 15 | 15 |
| y4 (1+) | 20.9 | 8.6 | 4.2 | 31.1 | 10.9 | 4.3 | 37.5 | 13.9 | 6 | 15 | 15 | 15 |
| y5 (1+) | 18.1 | 10 | 6 | 17.8 | 9.9 | 8 | 25.4 | 14.1 | 10 | 15 | 15 | 15 |
| sum | 6.7 | 3.4 | 4.6 | 14.7 | 6.1 | 4.6 | 16.2 | 7 | 6.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-1085 Collapse assay details
Data source: Panorama
Official Gene Symbol
APOC1
Peptide Sequence
TPDVSSALDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
27
Peptide End
36
CPTAC ID
non-CPTAC-1085
Peptide Molecular Mass
1,031.5135
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (2+) | 10.1 | 6.9 | 8 | 11.8 | 6.3 | 7.6 | 15.5 | 9.3 | 11 | 15 | 15 | 15 |
| y3 (1+) | 22.4 | 9.1 | 5.2 | 20 | 9.6 | 4.6 | 30 | 13.2 | 6.9 | 15 | 15 | 15 |
| y6 (1+) | 16.7 | 10 | 8.1 | 17.4 | 10.7 | 9.5 | 24.1 | 14.6 | 12.5 | 15 | 15 | 15 |
| y7 (1+) | 19.6 | 9.3 | 6.5 | 19.5 | 9.1 | 6.8 | 27.6 | 13 | 9.4 | 15 | 15 | 15 |
| y8 (1+) | 13.3 | 9.4 | 6.3 | 12.1 | 8.4 | 8.4 | 18 | 12.6 | 10.5 | 15 | 15 | 15 |
| sum | 8.7 | 4 | 4.9 | 9.2 | 4.6 | 5.2 | 12.7 | 6.1 | 7.1 | 15 | 15 | 15 |