C3, CPTAC-1105 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-5596: View additional GTLSVVAVYHAK data

non-CPTAC-1105: View additional TGLQEVEVK data

10 20 30 40 50
MGPTSGPSLL LLLLTHLPLA LGSPMYSIIT PNILRLESEE TMVLEAHDAQ
60 70 80 90 100
GDVPVTVTVH DFPGKKLVLS SEKTVLTPAT NHMGNVTFTI PANREFKSEK
110 120 130 140 150
GRNKFVTVQA TFGTQVVEKV VLVSLQSGYL FIQTDKTIYT PGSTVLYRIF
160 170 180 190 200
TVNHKLLPVG RTVMVNIENP EGIPVKQDSL SSQNQLGVLP LSWDIPELVN
210 220 230 240 250
MGQWKIRAYY ENSPQQVFST EFEVKEYVLP SFEVIVEPTE KFYYIYNEKG
260 270 280 290 300
LEVTITARFL YGKKVEGTAF VIFGIQDGEQ RISLPESLKR IPIEDGSGEV
310 320 330 340 350
VLSRKVLLDG VQNPRAEDLV GKSLYVSATV ILHSGSDMVQ AERSGIPIVT
360 370 380 390 400
SPYQIHFTKT PKYFKPGMPF DLMVFVTNPD GSPAYRVPVA VQGEDTVQSL
410 420 430 440 450
TQGDGVAKLS INTHPSQKPL SITVRTKKQE LSEAEQATRT MQALPYSTVG
460 470 480 490 500
NSNNYLHLSV LRTELRPGET LNVNFLLRMD RAHEAKIRYY TYLIMNKGRL
510 520 530 540 550
LKAGRQVREP GQDLVVLPLS ITTDFIPSFR LVAYYTLIGA SGQREVVADS
560 570 580 590 600
VWVDVKDSCV GSLVVKSGQS EDRQPVPGQQ MTLKIEGDHG ARVVLVAVDK
610 620 630 640 650
GVFVLNKKNK LTQSKIWDVV EKADIGCTPG SGKDYAGVFS DAGLTFTSSS
660 670 680 690 700
GQQTAQRAEL QCPQPAARRR RSVQLTEKRM DKVGKYPKEL RKCCEDGMRE
710 720 730 740 750
NPMRFSCQRR TRFISLGEAC KKVFLDCCNY ITELRRQHAR ASHLGLARSN
760 770 780 790 800
LDEDIIAEEN IVSRSEFPES WLWNVEDLKE PPKNGISTKL MNIFLKDSIT
810 820 830 840 850
TWEILAVSMS DKKGICVADP FEVTVMQDFF IDLRLPYSVV RNEQVEIRAV
860 870 880 890 900
LYNYRQNQEL KVRVELLHNP AFCSLATTKR RHQQTVTIPP KSSLSVPYVI
910 920 930 940 950
VPLKTGLQEV EVKAAVYHHF ISDGVRKSLK VVPEGIRMNK TVAVRTLDPE
960 970 980 990 1000
RLGREGVQKE DIPPADLSDQ VPDTESETRI LLQGTPVAQM TEDAVDAERL
1010 1020 1030 1040 1050
KHLIVTPSGC GEQNMIGMTP TVIAVHYLDE TEQWEKFGLE KRQGALELIK
1060 1070 1080 1090 1100
KGYTQQLAFR QPSSAFAAFV KRAPSTWLTA YVVKVFSLAV NLIAIDSQVL
1110 1120 1130 1140 1150
CGAVKWLILE KQKPDGVFQE DAPVIHQEMI GGLRNNNEKD MALTAFVLIS
1160 1170 1180 1190 1200
LQEAKDICEE QVNSLPGSIT KAGDFLEANY MNLQRSYTVA IAGYALAQMG
1210 1220 1230 1240 1250
RLKGPLLNKF LTTAKDKNRW EDPGKQLYNV EATSYALLAL LQLKDFDFVP
1260 1270 1280 1290 1300
PVVRWLNEQR YYGGGYGSTQ ATFMVFQALA QYQKDAPDHQ ELNLDVSLQL
1310 1320 1330 1340 1350
PSRSSKITHR IHWESASLLR SEETKENEGF TVTAEGKGQG TLSVVTMYHA
1360 1370 1380 1390 1400
KAKDQLTCNK FDLKVTIKPA PETEKRPQDA KNTMILEICT RYRGDQDATM
1410 1420 1430 1440 1450
SILDISMMTG FAPDTDDLKQ LANGVDRYIS KYELDKAFSD RNTLIIYLDK
1460 1470 1480 1490 1500
VSHSEDDCLA FKVHQYFNVE LIQPGAVKVY AYYNLEESCT RFYHPEKEDG
1510 1520 1530 1540 1550
KLNKLCRDEL CRCAEENCFI QKSDDKVTLE ERLDKACEPG VDYVYKTRLV
1560 1570 1580 1590 1600
KVQLSNDFDE YIMAIEQTIK SGSDEVQVGQ QRTFISPIKC REALKLEEKK
1610 1620 1630 1640 1650
HYLMWGLSSD FWGEKPNLSY IIGKDTWVEH WPEEDECQDE ENQKQCQDLG
1660 1663
AFTESMVVFG CPN

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1239 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

TIYTPGSTVLYR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

137

Peptide End

148

CPTAC ID

CPTAC-1239

Peptide Molecular Mass

1,369.7242

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

Ovarian cancer tumor tissue lysate

Submitting Laboratory

Pacific Northwest National Laboratory

Submitting Lab PI

Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Waters nanoACQUITY (Part Number 176016000)

Column Packing

Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)

Column Dimensions

100 um x 100 mm

Flow Rate

0.5 uL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 8.6 | 5.6 | 8.4 | 15.6 | 9 | 8.5 | 17.8 | 10.6 | 12 | 15 | 15 | 15 | | y9 (1+) | 35.1 | 8.5 | 10.1 | 24.5 | 11.2 | 10.5 | 42.8 | 14.1 | 14.6 | 15 | 15 | 15 | | y10 (1+) | 23.9 | 6.2 | 7.9 | 23.4 | 7.4 | 10.3 | 33.4 | 9.7 | 13 | 15 | 15 | 15 | | sum | 12.9 | 5.6 | 8 | 13.8 | 8.2 | 8.8 | 18.9 | 9.9 | 11.9 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-5596 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

GTLSVVAVYHAK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

1340

Peptide End

1351

CPTAC ID

non-CPTAC-5596

Peptide Molecular Mass

1,243.6925

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (3+) | 19.7 | 10 | 9.5 | 28.6 | 12.5 | 10 | 34.7 | 16 | 13.8 | 15 | 15 | 15 | | y9 (2+) | 6.2 | 3.2 | 3.9 | 6.9 | 5 | 4.3 | 9.3 | 5.9 | 5.8 | 15 | 15 | 15 | | y10 (2+) | 6.8 | 5.1 | 3.8 | 7.9 | 7 | 5.1 | 10.4 | 8.7 | 6.4 | 15 | 15 | 15 | | y4 (1+) | 7.8 | 6.9 | 2.7 | 9.8 | 6.6 | 4.2 | 12.5 | 9.5 | 5 | 15 | 15 | 15 | | y6 (1+) | 6.9 | 3.1 | 2.2 | 10.6 | 6.6 | 2.9 | 12.6 | 7.3 | 3.6 | 15 | 15 | 15 | | sum | 3.5 | 2.9 | 1.6 | 5.4 | 5.6 | 2.7 | 6.4 | 6.3 | 3.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-1106 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

SGIPIVTSPYQIHFTK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

344

Peptide End

359

CPTAC ID

non-CPTAC-1106

Peptide Molecular Mass

1,786.9618

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b14 (3+) | NA | NA | NA | NA | NA | NA | NA | NA | NA | 0 | 0 | 0 | | b3 (1+) | 5.5 | 3 | 2.7 | 14.3 | 7.1 | 6 | 15.3 | 7.7 | 6.6 | 15 | 15 | 15 | | y13 (3+) | 5.9 | 3.4 | 4.3 | 13.5 | 7.1 | 5.5 | 14.7 | 7.9 | 7 | 15 | 15 | 15 | | y13 (2+) | 6 | 4.5 | 3.6 | 13.2 | 7.4 | 5.8 | 14.5 | 8.7 | 6.8 | 15 | 15 | 15 | | sum | 5.4 | 1.7 | 2.9 | 13.1 | 5.9 | 5 | 14.2 | 6.1 | 5.8 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-1105 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

TGLQEVEVK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

905

Peptide End

913

CPTAC ID

non-CPTAC-1105

Peptide Molecular Mass

1,001.5393

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (2+) | 6.5 | 4.8 | 5 | 13 | 6.4 | 6.6 | 14.5 | 8 | 8.3 | 15 | 15 | 15 | | y2 (1+) | 4.9 | 4.5 | 4.5 | 8.8 | 6.5 | 4.2 | 10.1 | 7.9 | 6.2 | 15 | 15 | 15 | | y5 (1+) | 5.7 | 6.8 | 3.8 | 8.4 | 9.1 | 7 | 10.2 | 11.4 | 8 | 15 | 15 | 15 | | y6 (1+) | 6.3 | 4.2 | 5 | 11.7 | 9.5 | 6.1 | 13.3 | 10.4 | 7.9 | 15 | 15 | 15 | | sum | 2.7 | 2 | 2.1 | 8.9 | 6.4 | 3.5 | 9.3 | 6.7 | 4.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-2653 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

VHQYFNVELIQPGAVK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

1463

Peptide End

1478

CPTAC ID

non-CPTAC-2653

Peptide Molecular Mass

1,840.9836

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 9.7 | 4.9 | 3.5 | 17 | 9 | 6.6 | 19.6 | 10.2 | 7.5 | 15 | 15 | 15 | | b6 (1+) | 8.5 | 7.7 | 4.9 | 13 | 7.2 | 8.7 | 15.5 | 10.5 | 10 | 15 | 15 | 15 | | y6 (1+) | 6.2 | 4.8 | 5.4 | 17 | 9.8 | 5.7 | 18.1 | 10.9 | 7.9 | 15 | 15 | 15 | | y7 (1+) | 9.4 | 6.9 | 3.6 | 17 | 8.2 | 5.3 | 19.4 | 10.7 | 6.4 | 15 | 15 | 15 | | b13 (3+) | 11.9 | 5.9 | 6.3 | 17.4 | 7.2 | 7.1 | 21.1 | 9.3 | 9.5 | 15 | 15 | 15 | | sum | 5.8 | 3.2 | 2.6 | 13.2 | 6.1 | 4.2 | 14.4 | 6.9 | 4.9 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-5595 Collapse assay details

Data source: Panorama

Official Gene Symbol

C3

Peptide Sequence

VHQYFNVGLIQPGSVK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

1463

Peptide End

1478

CPTAC ID

non-CPTAC-5595

Peptide Molecular Mass

1,784.9574

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b9 (2+) | 5.1 | 2.7 | 2.9 | 7 | 6.7 | 3.4 | 8.7 | 7.2 | 4.5 | 15 | 15 | 15 | | b6 (1+) | 4.6 | 3.8 | 5 | 9 | 6.8 | 4 | 10.1 | 7.8 | 6.4 | 15 | 15 | 15 | | b2 (1+) | 4.6 | 2.7 | 2.8 | 6.6 | 6.4 | 3.6 | 8 | 6.9 | 4.6 | 15 | 15 | 15 | | y5 (1+) | 4.6 | 1.5 | 4.2 | 6.2 | 5.1 | 5.8 | 7.7 | 5.3 | 7.2 | 15 | 15 | 15 | | y7 (1+) | 3.5 | 3.8 | 3.3 | 8.4 | 6.8 | 2.8 | 9.1 | 7.8 | 4.3 | 15 | 15 | 15 | | sum | 1 | 1 | 1.2 | 5.9 | 5.4 | 2.7 | 6 | 5.5 | 3 | 15 | 15 | 15 |

Additional Resources and Comments