SERPINE1, CPTAC-1150 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-4049: View additional FSLETEVDLR data

non-CPTAC-1150: View additional VFQQVAQASK data

10 20 30 40 50
MQMSPALTCL VLGLALVFGE GSAVHHPPSY VAHLASDFGV RVFQQVAQAS
60 70 80 90 100
KDRNVVFSPY GVASVLAMLQ LTTGGETQQQ IQAAMGFKID DKGMAPALRH
110 120 130 140 150
LYKELMGPWN KDEISTTDAI FVQRDLKLVQ GFMPHFFRLF RSTVKQVDFS
160 170 180 190 200
EVERARFIIN DWVKTHTKGM ISNLLGKGAV DQLTRLVLVN ALYFNGQWKT
210 220 230 240 250
PFPDSSTHRR LFHKSDGSTV SVPMMAQTNK FNYTEFTTPD GHYYDILELP
260 270 280 290 300
YHGDTLSMFI AAPYEKEVPL SALTNILSAQ LISHWKGNMT RLPRLLVLPK
310 320 330 340 350
FSLETEVDLR KPLENLGMTD MFRQFQADFT SLSDQEPLHV AQALQKVKIE
360 370 380 390 400
VNESGTVASS STAVIVSARM APEEIIMDRP FLFVVRHNPT GTVLFMGQVM
402
EP

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4049 Collapse assay details

Data source: Panorama

Official Gene Symbol

Serpine1

Peptide Sequence

FSLETEVDLR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

301

Peptide End

310

CPTAC ID

non-CPTAC-4049

Peptide Molecular Mass

1,207.6085

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 11.3 | 9.4 | 5.3 | 10.4 | 10 | 7 | 15.4 | 13.7 | 8.8 | 15 | 15 | 15 | | y7 (1+) | 11.9 | 9.4 | 7.3 | 12.3 | 10 | 8.2 | 17.1 | 13.7 | 11 | 15 | 15 | 15 | | y6 (1+) | 16.5 | 20.4 | 19 | 21 | 23.2 | 17.6 | 26.7 | 30.9 | 25.9 | 15 | 15 | 15 | | y2 (1+) | 9.9 | 6.7 | 6.1 | 10 | 7.6 | 5.3 | 14.1 | 10.1 | 8.1 | 15 | 15 | 15 | | y3 (2+) | 104.5 | 43.4 | 75.4 | 116.9 | 93.9 | 75.2 | 156.8 | 103.4 | 106.5 | 15 | 15 | 15 | | sum | 9.1 | 8.7 | 7.4 | 10.4 | 8.8 | 7 | 13.8 | 12.4 | 10.2 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-1150 Collapse assay details

Data source: Panorama

Official Gene Symbol

SERPINE1

Peptide Sequence

VFQQVAQASK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

42

Peptide End

51

CPTAC ID

non-CPTAC-1150

Peptide Molecular Mass

1,104.5928

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b2 (1+) | 12.1 | 5.1 | 8.5 | 15.6 | 8.3 | 11 | 19.7 | 9.7 | 13.9 | 14 | 15 | 15 | | y8 (2+) | 17.5 | 11.7 | 9.6 | 17.1 | 10.6 | 12.7 | 24.5 | 15.8 | 15.9 | 15 | 15 | 15 | | y5 (1+) | 9.7 | 10.6 | 7.4 | 8.9 | 13.6 | 8.2 | 13.2 | 17.2 | 11 | 15 | 15 | 15 | | y7 (1+) | 28.7 | 10.3 | 7.5 | 23.1 | 12.4 | 8.8 | 36.8 | 16.1 | 11.6 | 15 | 15 | 15 | | y8 (1+) | 16.9 | 11 | 7 | 16.9 | 10.5 | 7.4 | 23.9 | 15.2 | 10.2 | 15 | 15 | 15 | | sum | 8.6 | 5.3 | 4.8 | 7.6 | 5.9 | 4.5 | 11.5 | 7.9 | 6.6 | 15 | 15 | 15 |

Additional Resources and Comments