TFPI, CPTAC-1163 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-1163: View additional FYYNSVIGK data

10 20 30 40 50
MIYTMKKVHA LWASVCLLLN LAPAPLNADS EEDEEHTIIT DTELPPLKLM
60 70 80 90 100
HSFCAFKADD GPCKAIMKRF FFNIFTRQCE EFIYGGCEGN QNRFESLEEC
110 120 130 140 150
KKMCTRDNAN RIIKTTLQQE KPDFCFLEED PGICRGYITR YFYNNQTKQC
160 170 180 190 200
ERFKYGGCLG NMNNFETLEE CKNICEDGPN GFQVDNYGTQ LNAVNNSLTP
210 220 230 240 250
QSTKVPSLFE FHGPSWCLTP ADRGLCRANE NRFYYNSVIG KCRPFKYSGC
260 270 280 290 300
GGNENNFTSK QECLRACKKG FIQRISKGGL IKTKRKRKKQ RVKIAYEEIF
304
VKNM

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1163 Collapse assay details

Data source: Panorama

Official Gene Symbol

TFPI

Peptide Sequence

FYYNSVIGK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

233

Peptide End

241

CPTAC ID

non-CPTAC-1163

Peptide Molecular Mass

1,089.5495

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b2 (1+) | 12.2 | 10.6 | 6.2 | 12.7 | 10.8 | 5.9 | 17.6 | 15.1 | 8.6 | 15 | 15 | 15 | | y3 (1+) | 27.8 | 9.4 | 6.2 | 31 | 12.9 | 5.2 | 41.6 | 16 | 8.1 | 15 | 15 | 15 | | y6 (1+) | 19.9 | 7.7 | 8.5 | 24.2 | 8.4 | 10.4 | 31.3 | 11.4 | 13.4 | 13 | 15 | 15 | | y7 (1+) | 10.2 | 9 | 6.1 | 13.1 | 9.2 | 6.1 | 16.6 | 12.9 | 8.6 | 15 | 15 | 15 | | y8 (1+) | 23.5 | 8.2 | 9.6 | 24.9 | 9.6 | 9.1 | 34.2 | 12.6 | 13.2 | 14 | 15 | 15 | | sum | 5.8 | 7.3 | 4.3 | 6 | 7.4 | 4.3 | 8.3 | 10.4 | 6.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-4064 Collapse assay details

Data source: Panorama

Official Gene Symbol

Tfpi

Peptide Sequence

STVNNIVVPQSPK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

200

Peptide End

212

CPTAC ID

non-CPTAC-4064

Peptide Molecular Mass

1,381.7565

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 3.6 | 5.6 | 4.9 | 11.3 | 6.1 | 5.4 | 11.9 | 8.3 | 7.3 | 15 | 15 | 15 | | y6 (1+) | 4.7 | 7.2 | 4 | 15 | 7.2 | 7.4 | 15.7 | 10.2 | 8.4 | 15 | 15 | 15 | | y5 (1+) | 5.5 | 4.5 | 2.8 | 11.5 | 5.4 | 4.6 | 12.7 | 7 | 5.4 | 15 | 15 | 15 | | y3 (1+) | 17.8 | 7 | 10.1 | 18.8 | 8.3 | 11.3 | 25.9 | 10.9 | 15.2 | 15 | 15 | 15 | | y2 (1+) | 9.1 | 6.2 | 9.7 | 12.8 | 8.5 | 9.3 | 15.7 | 10.5 | 13.4 | 15 | 15 | 15 | | sum | 3 | 3.8 | 2 | 10 | 4.3 | 4 | 10.4 | 5.7 | 4.5 | 15 | 15 | 15 |

Additional Resources and Comments