F5, CPTAC-2648 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-2648: View additional SEAYNTFSER data

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2648 Collapse assay details

Data source: Panorama

Official Gene Symbol

F5

Peptide Sequence

SEAYNTFSER

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

1053

Peptide End

1062

CPTAC ID

non-CPTAC-2648

Peptide Molecular Mass

1,202.5204

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 7.1 | 9 | 10.2 | 9.6 | 9.9 | 14.1 | 11.9 | 13.4 | 17.4 | 15 | 15 | 15 | | y6 (1+) | 13.1 | 7.9 | 9 | 18.4 | 8.5 | 11.7 | 22.6 | 11.6 | 14.8 | 15 | 15 | 15 | | y8 (2+) | 24.7 | 11 | 9.9 | 29.1 | 17.8 | 13.2 | 38.2 | 20.9 | 16.5 | 15 | 15 | 15 | | b3 (1+) | 11.3 | 9 | 10.3 | 17.4 | 11.7 | 8.7 | 20.7 | 14.8 | 13.5 | 15 | 15 | 15 | | y3 (1+) | 20.5 | 8.8 | 8 | 20.6 | 9.9 | 10.5 | 29.1 | 13.2 | 13.2 | 15 | 15 | 15 | | sum | 6.4 | 4.6 | 6.3 | 11.2 | 8 | 8.6 | 12.9 | 9.2 | 10.7 | 15 | 15 | 15 |

Additional Resources and Comments