CFH, CPTAC-2658 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
non-CPTAC-2658: View additional SSQESYAHGTK data
non-CPTAC-5588: View additional TGEQVTFR data
non-CPTAC-3900: View additional VEYSHGEVVK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MRLLAKIICL | MLWAICVAED | CNELPPRRNT | EILTGSWSDQ | TYPEGTQAIY |
| 60 | 70 | 80 | 90 | 100 |
| KCRPGYRSLG | NVIMVCRKGE | WVALNPLRKC | QKRPCGHPGD | TPFGTFTLTG |
| 110 | 120 | 130 | 140 | 150 |
| GNVFEYGVKA | VYTCNEGYQL | LGEINYRECD | TDGWTNDIPI | CEVVKCLPVT |
| 160 | 170 | 180 | 190 | 200 |
| APENGKIVSS | AMEPDREYHF | GQAVRFVCNS | GYKIEGDEEM | HCSDDGFWSK |
| 210 | 220 | 230 | 240 | 250 |
| EKPKCVEISC | KSPDVINGSP | ISQKIIYKEN | ERFQYKCNMG | YEYSERGDAV |
| 260 | 270 | 280 | 290 | 300 |
| CTESGWRPLP | SCEEKSCDNP | YIPNGDYSPL | RIKHRTGDEI | TYQCRNGFYP |
| 310 | 320 | 330 | 340 | 350 |
| ATRGNTAKCT | STGWIPAPRC | TLKPCDYPDI | KHGGLYHENM | RRPYFPVAVG |
| 360 | 370 | 380 | 390 | 400 |
| KYYSYYCDEH | FETPSGSYWD | HIHCTQDGWS | PAVPCLRKCY | FPYLENGYNQ |
| 410 | 420 | 430 | 440 | 450 |
| NYGRKFVQGK | SIDVACHPGY | ALPKAQTTVT | CMENGWSPTP | RCIRVKTCSK |
| 460 | 470 | 480 | 490 | 500 |
| SSIDIENGFI | SESQYTYALK | EKAKYQCKLG | YVTADGETSG | SITCGKDGWS |
| 510 | 520 | 530 | 540 | 550 |
| AQPTCIKSCD | IPVFMNARTK | NDFTWFKLND | TLDYECHDGY | ESNTGSTTGS |
| 560 | 570 | 580 | 590 | 600 |
| IVCGYNGWSD | LPICYERECE | LPKIDVHLVP | DRKKDQYKVG | EVLKFSCKPG |
| 610 | 620 | 630 | 640 | 650 |
| FTIVGPNSVQ | CYHFGLSPDL | PICKEQVQSC | GPPPELLNGN | VKEKTKEEYG |
| 660 | 670 | 680 | 690 | 700 |
| HSEVVEYYCN | PRFLMKGPNK | IQCVDGEWTT | LPVCIVEEST | CGDIPELEHG |
| 710 | 720 | 730 | 740 | 750 |
| WAQLSSPPYY | YGDSVEFNCS | ESFTMIGHRS | ITCIHGVWTQ | LPQCVAIDKL |
| 760 | 770 | 780 | 790 | 800 |
| KKCKSSNLII | LEEHLKNKKE | FDHNSNIRYR | CRGKEGWIHT | VCINGRWDPE |
| 810 | 820 | 830 | 840 | 850 |
| VNCSMAQIQL | CPPPPQIPNS | HNMTTTLNYR | DGEKVSVLCQ | ENYLIQEGEE |
| 860 | 870 | 880 | 890 | 900 |
| ITCKDGRWQS | IPLCVEKIPC | SQPPQIEHGT | INSSRSSQES | YAHGTKLSYT |
| 910 | 920 | 930 | 940 | 950 |
| CEGGFRISEE | NETTCYMGKW | SSPPQCEGLP | CKSPPEISHG | VVAHMSDSYQ |
| 960 | 970 | 980 | 990 | 1000 |
| YGEEVTYKCF | EGFGIDGPAI | AKCLGEKWSH | PPSCIKTDCL | SLPSFENAIP |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| MGEKKDVYKA | GEQVTYTCAT | YYKMDGASNV | TCINSRWTGR | PTCRDTSCVN |
| 1060 | 1070 | 1080 | 1090 | 1100 |
| PPTVQNAYIV | SRQMSKYPSG | ERVRYQCRSP | YEMFGDEEVM | CLNGNWTEPP |
| 1110 | 1120 | 1130 | 1140 | 1150 |
| QCKDSTGKCG | PPPPIDNGDI | TSFPLSVYAP | ASSVEYQCQN | LYQLEGNKRI |
| 1160 | 1170 | 1180 | 1190 | 1200 |
| TCRNGQWSEP | PKCLHPCVIS | REIMENYNIA | LRWTAKQKLY | SRTGESVEFV |
| 1210 | 1220 | 1230 | 1231 | |
| CKRGYRLSSR | SHTLRTTCWD | GKLEYPTCAK | R |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
loading
Assay Details for non-CPTAC-2658 Collapse assay details
Data source: Panorama
Official Gene Symbol
CFH
Peptide Sequence
SSQESYAHGTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
886
Peptide End
896
CPTAC ID
non-CPTAC-2658
Peptide Molecular Mass
1,193.5313
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 17.3 | 4.6 | 3.6 | 20.1 | 8.8 | 5.6 | 26.5 | 9.9 | 6.7 | 15 | 15 | 15 |
| y9 (2+) | 3.9 | 3.2 | 2.2 | 8.9 | 6 | 3.2 | 9.7 | 6.8 | 3.9 | 15 | 15 | 15 |
| y3 (1+) | 10.1 | 7.3 | 5.6 | 13.5 | 8 | 7.7 | 16.9 | 10.8 | 9.5 | 15 | 15 | 15 |
| y10 (2+) | 17.2 | 8.4 | 5.3 | 20.1 | 11.6 | 7.6 | 26.5 | 14.3 | 9.3 | 15 | 15 | 15 |
| y7 (2+) | 23.7 | 12 | 9.3 | 27.6 | 24.2 | 17.7 | 36.4 | 27 | 20 | 15 | 15 | 15 |
| sum | 3.4 | 4.1 | 3.4 | 5.8 | 4 | 5.3 | 6.7 | 5.7 | 6.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5588 Collapse assay details
Data source: Panorama
Official Gene Symbol
Cfh
Peptide Sequence
TGEQVTFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1015
Peptide End
1022
CPTAC ID
non-CPTAC-5588
Peptide Molecular Mass
936.4665
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 24.1 | 7.1 | 1.7 | 96.5 | 58.6 | 2.8 | 99.5 | 59 | 3.3 | 15 | 15 | 15 |
| y3 (1+) | 3 | 2.3 | 2.3 | 6.7 | 6.1 | 3.6 | 7.3 | 6.5 | 4.3 | 15 | 15 | 15 |
| y4 (1+) | 2 | 2.1 | 2 | 9.4 | 5.4 | 3.2 | 9.6 | 5.8 | 3.8 | 15 | 15 | 15 |
| y5 (1+) | 2.6 | 1.1 | 2.1 | 9 | 7 | 3.2 | 9.4 | 7.1 | 3.8 | 15 | 15 | 15 |
| y7 (1+) | 3.3 | 2.4 | 2.6 | 6.1 | 6 | 2.8 | 6.9 | 6.5 | 3.8 | 15 | 15 | 15 |
| sum | 13.5 | 2.3 | 0.9 | 68.8 | 13.3 | 2.6 | 70.1 | 13.5 | 2.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3900 Collapse assay details
Data source: Panorama
Official Gene Symbol
Cfh
Peptide Sequence
VEYSHGEVVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
646
Peptide End
655
CPTAC ID
non-CPTAC-3900
Peptide Molecular Mass
1,145.5717
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (2+) | 9.3 | 7.1 | 2.6 | 13.8 | 8.4 | 4.9 | 16.6 | 11 | 5.5 | 15 | 15 | 15 |
| y8 (2+) | 7.1 | 5.2 | 4.1 | 6.3 | 6.7 | 7.5 | 9.5 | 8.5 | 8.5 | 15 | 15 | 15 |
| y7 (2+) | 23.8 | 9 | 7.4 | 22.7 | 10.4 | 11.4 | 32.9 | 13.8 | 13.6 | 15 | 15 | 15 |
| b2 (1+) | 9.5 | 7.3 | 6.2 | 12.2 | 6.3 | 8.2 | 15.5 | 9.6 | 10.3 | 15 | 15 | 15 |
| b6 (3+) | 24.7 | 11 | 5.4 | 21 | 8.6 | 5.8 | 32.4 | 14 | 7.9 | 15 | 15 | 15 |
| sum | 5.5 | 4.1 | 1.7 | 5 | 5.5 | 5.8 | 7.4 | 6.9 | 6 | 15 | 15 | 15 |