MST1, CPTAC-2672 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

non-CPTAC-2672: View additional SPLNDFQVLR data

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6061 Collapse assay details

Data source: Panorama

Official Gene Symbol

MST1

Peptide Sequence

VVGGHPGNSPWTVSLR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

484

Peptide End

499

CPTAC ID

CPTAC-6061

Peptide Molecular Mass

1,661.8638

Species

Human

Assay Type

Enrichment MRM

Enrichment Method

N/A

Matrix

FFPE lung tumor tissue lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

QTRAP 5500

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Eksigent nanoLC 425

Column Packing

3 µm ChromXP C18EP, 120 Å

Column Dimensions

150 x 0.075 mm

Flow Rate

0.3 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y3 (1+) | 27.1 | 16.7 | 18.4 | 26.2 | 19.6 | 31.5 | 37.7 | 25.7 | 36.5 | 15 | 15 | 15 | | y4 (1+) | 18.2 | 12.1 | 19 | 18.7 | 12.7 | 27.2 | 26.1 | 17.5 | 33.2 | 15 | 15 | 15 | | y5 (1+) | 19.5 | 8.3 | 21 | 19.1 | 11.9 | 23.7 | 27.3 | 14.5 | 31.7 | 15 | 15 | 15 | | y6 (1+) | 13.7 | 13.5 | 26 | 18.6 | 17.3 | 27.3 | 23.1 | 21.9 | 37.7 | 15 | 15 | 15 | | y7 (1+) | 19.1 | 9.1 | 35 | 20.5 | 11 | 37.4 | 28 | 14.3 | 51.2 | 15 | 15 | 15 | | y7 (2+) | 20.9 | 12.4 | 24.2 | 19.8 | 12.4 | 29.9 | 28.8 | 17.5 | 38.5 | 15 | 15 | 15 | | sum | 16 | 7.9 | 14.9 | 15.5 | 9.5 | 22.2 | 22.3 | 12.4 | 26.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-2672 Collapse assay details

Data source: Panorama

Official Gene Symbol

MST1

Peptide Sequence

SPLNDFQVLR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

21

Peptide End

30

CPTAC ID

non-CPTAC-2672

Peptide Molecular Mass

1,187.6299

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y6 (1+) | 6.6 | 6.5 | 10.9 | 13.7 | 9.5 | 10.6 | 15.2 | 11.5 | 15.2 | 15 | 15 | 15 | | y5 (1+) | 13.2 | 6.7 | 9.1 | 16.1 | 12.2 | 11.7 | 20.8 | 13.9 | 14.8 | 15 | 15 | 15 | | y3 (1+) | 14.7 | 6.3 | 10.3 | 15.3 | 8.8 | 13.2 | 21.2 | 10.8 | 16.7 | 15 | 15 | 15 | | y9 (2+) | 17.6 | 6.3 | 7.4 | 20 | 9.2 | 11.1 | 26.6 | 11.2 | 13.3 | 15 | 15 | 15 | | y7 (1+) | 9.2 | 6.2 | 6.4 | 11.3 | 8.4 | 9.4 | 14.6 | 10.4 | 11.4 | 15 | 15 | 15 | | sum | 8.2 | 4 | 4 | 12.2 | 7.9 | 8.5 | 14.7 | 8.9 | 9.4 | 15 | 15 | 15 |

Additional Resources and Comments