IGFALS, CPTAC-2678 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

non-CPTAC-5574: View additional DFALQNPGVVPR data

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Phosphorylation Acetylation Ubiquitylation Other

loading

Assay Details for non-CPTAC-5574 Collapse assay details

Data source: Panorama

Official Gene Symbol

Igfals

Peptide Sequence

DFALQNPGVVPR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

548

Peptide End

559

CPTAC ID

non-CPTAC-5574

Peptide Molecular Mass

1,311.6935

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b3 (1+) | 9.8 | 6.6 | 9.6 | 9.6 | 10.2 | 9.7 | 13.7 | 12.1 | 13.6 | 15 | 15 | 15 | | b2 (1+) | 17 | 9.3 | 8.9 | 13.7 | 8.4 | 8.4 | 21.8 | 12.5 | 12.2 | 15 | 15 | 15 | | y2 (1+) | 10.1 | 6.4 | 5.3 | 13.2 | 11.1 | 7.8 | 16.6 | 12.8 | 9.4 | 15 | 15 | 15 | | y3 (1+) | 10 | 8.3 | 7.7 | 12.8 | 7.6 | 8.5 | 16.2 | 11.3 | 11.5 | 15 | 15 | 15 | | y6 (1+) | 7.6 | 6.9 | 6.7 | 13 | 9.5 | 5.3 | 15.1 | 11.7 | 8.5 | 15 | 15 | 15 | | sum | 4 | 1.5 | 2.5 | 7 | 6.3 | 3.7 | 8.1 | 6.5 | 4.5 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-2678 Collapse assay details

Data source: Panorama

Official Gene Symbol

IGFALS

Peptide Sequence

SLALGTFAHTPALASLGLSNNR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

136

Peptide End

157

CPTAC ID

non-CPTAC-2678

Peptide Molecular Mass

2,210.1808

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

6490 Triple Quad (Agilent)

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y6 (1+) | 5.4 | 4.7 | 16.9 | 19.1 | 7.8 | 23.7 | 19.8 | 9.1 | 29.1 | 15 | 15 | 15 | | b2 (1+) | 5.8 | 4.2 | 10 | 18.7 | 7.3 | 13.2 | 19.6 | 8.4 | 16.6 | 15 | 15 | 15 | | b14 (2+) | 9.2 | 5.2 | 14 | 18.3 | 8 | 19.6 | 20.5 | 9.5 | 24.1 | 15 | 15 | 15 | | b6 (2+) | 7 | 4.3 | 12.1 | 18.7 | 7.1 | 15.3 | 20 | 8.3 | 19.5 | 15 | 15 | 15 | | b17 (2+) | 5.7 | 4.4 | 16.8 | 20.1 | 8.6 | 22.8 | 20.9 | 9.7 | 28.3 | 15 | 15 | 15 | | sum | 5.7 | 4.3 | 13.7 | 18.9 | 7.4 | 18.3 | 19.7 | 8.6 | 22.9 | 15 | 15 | 15 |

Additional Resources and Comments