Mpo, CPTAC-3375 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-3375: View additional IANVFTNAFR data

10 20 30 40 50
MKLLLALAGL LAPLAMLQTS NGATPALLGE VENSVVLSCM EEAKQLVDRA
60 70 80 90 100
YKERRESIKR SLQSGSASPT ELLFYFKQPV AGTRTAVRAA DYLHVALDLL
110 120 130 140 150
KRKLQPLWPR PFNVTDVLTP AQLNLLSVSS GCAYQDVRVT CPPNDKYRTI
160 170 180 190 200
TGHCNNRRSP TLGASNRAFV RWLPAEYEDG VSMPFGWTPG VNRNGFKVPL
210 220 230 240 250
ARQVSNAIVR FPNDQLTKDQ ERALMFMQWG QFLDHDITLT PEPATRFSFF
260 270 280 290 300
TGLNCETSCL QQPPCFPLKI PPNDPRIKNQ KDCIPFFRSC PACTRNNITI
310 320 330 340 350
RNQINALTSF VDASGVYGSE DPLARKLRNL TNQLGLLAIN TRFQDNGRAL
360 370 380 390 400
MPFDSLHDDP CLLTNRSARI PCFLAGDMRS SEMPELTSMH TLFVREHNRL
410 420 430 440 450
ATQLKRLNPR WNGEKLYQEA RKIVGAMVQI ITYRDYLPLV LGPAAMKKYL
460 470 480 490 500
PQYRSYNDSV DPRIANVFTN AFRYGHTLIQ PFMFRLNNQY RPTGPNPRVP
510 520 530 540 550
LSKVFFASWR VVLEGGIDPI LRGLMATPAK LNRQNQIVVD EIRERLFEQV
560 570 580 590 600
MRIGLDLPAL NMQRSRDHGL PGYNAWRRFC GLPQPSTVGE LGTVLKNLEL
610 620 630 640 650
ARKLMAQYGT PNNIDIWMGG VSEPLEPNGR VGQLLACLIG TQFRKLRDGD
660 670 680 690 700
RFWWENPGVF SKQQRQALAS ISLPRIICDN TGITTVSKNN IFMSNTYPRD
710 718
FVSCNTLPKL NLTSWKET

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6037 Collapse assay details

Data source: Panorama

Official Gene Symbol

MPO

Peptide Modified Sequence

IGLDLPALNM[+15.994915]QR

Modification Type

Oxidation (M)

Protein - Site of Modification

588

Peptide - Site of Modification

10

Peptide Start

579

Peptide End

590

CPTAC ID

CPTAC-6037

Peptide Molecular Mass

1,355.7231

Species

Human

Assay Type

Enrichment MRM

Enrichment Method

N/A

Matrix

FFPE lung tumor tissue lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

QTRAP 5500

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Eksigent nanoLC 425

Column Packing

3 µm ChromXP C18EP, 120 Å

Column Dimensions

150 x 0.075 mm

Flow Rate

0.3 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b4 (1+) | 61 | 67.6 | 40.5 | 70.7 | 86.3 | 59.5 | 93.4 | 109.6 | 72 | 15 | 15 | 15 | | b5 (1+) | 41.8 | 60.7 | 58.9 | 69.1 | 59.7 | 75.7 | 80.8 | 85.1 | 95.9 | 15 | 15 | 15 | | y4 (1+) | 78.4 | 71.2 | 58.6 | 84.2 | 72.7 | 72.7 | 115 | 101.8 | 93.4 | 15 | 15 | 15 | | y6 (1+) | 72.4 | 70.4 | 53.7 | 79.1 | 87.5 | 76.1 | 107.2 | 112.3 | 93.1 | 15 | 15 | 15 | | y7 (1+) | 42.9 | 62.4 | 33.3 | 42.4 | 74.9 | 40.6 | 60.3 | 97.5 | 52.5 | 15 | 15 | 15 | | y8 (1+) | 48.6 | 68.8 | 31.2 | 45.1 | 67.7 | 74 | 66.3 | 96.5 | 80.3 | 15 | 15 | 15 | | y9 (1+) | 54 | 85.5 | 44.5 | 56.4 | 83.4 | 64.3 | 78.1 | 119.4 | 78.2 | 15 | 15 | 15 | | sum | 34.9 | 62 | 32.4 | 32 | 71.4 | 48.1 | 47.3 | 94.6 | 58 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-6053 Collapse assay details

Data source: Panorama

Official Gene Symbol

MPO

Peptide Sequence

IGLDLPALNMQR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

579

Peptide End

590

CPTAC ID

CPTAC-6053

Peptide Molecular Mass

1,339.7282

Species

Human

Assay Type

Enrichment MRM

Enrichment Method

N/A

Matrix

FFPE lung tumor tissue lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

QTRAP 5500

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Eksigent nanoLC 425

Column Packing

3 µm ChromXP C18EP, 120 Å

Column Dimensions

150 x 0.075 mm

Flow Rate

0.3 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b5 (1+) | 20.5 | 20.8 | 19.8 | 18.4 | 22.7 | 23.4 | 27.5 | 30.8 | 30.7 | 15 | 15 | 15 | | y7 (1+) | 11 | 24.3 | 17.1 | 12.2 | 24.3 | 17.9 | 16.4 | 34.4 | 24.8 | 15 | 15 | 15 | | y8 (1+) | 9.3 | 22.3 | 20.4 | 16.2 | 26 | 27.3 | 18.7 | 34.3 | 34.1 | 15 | 15 | 15 | | y9 (1+) | 17.9 | 24.9 | 24.6 | 18.4 | 29 | 25.9 | 25.7 | 38.2 | 35.7 | 15 | 15 | 15 | | sum | 10.7 | 23.8 | 16.9 | 12 | 24.1 | 18 | 16.1 | 33.9 | 24.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-3375 Collapse assay details

Data source: Panorama

Official Gene Symbol

Mpo

Peptide Sequence

IANVFTNAFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

464

Peptide End

473

CPTAC ID

non-CPTAC-3375

Peptide Molecular Mass

1,151.6087

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b4 (1+) | 11.9 | 6.6 | 3.9 | 19.1 | 10.3 | 4.9 | 22.5 | 12.2 | 6.3 | 15 | 15 | 15 | | b3 (1+) | 11.7 | 4 | 5.8 | 26.9 | 12.8 | 7.1 | 29.3 | 13.4 | 9.2 | 15 | 15 | 15 | | y5 (1+) | 3.5 | 6.5 | 6 | 13.7 | 11.3 | 6.8 | 14.1 | 13 | 9.1 | 15 | 15 | 15 | | y6 (1+) | 5.8 | 6.7 | 3.6 | 13.6 | 11.7 | 4.7 | 14.8 | 13.5 | 5.9 | 15 | 15 | 15 | | y8 (1+) | 5.5 | 5.2 | 5.7 | 13.3 | 7.9 | 6.2 | 14.4 | 9.5 | 8.4 | 15 | 15 | 15 | | sum | 2.6 | 3 | 1.5 | 14.4 | 9.5 | 3.8 | 14.6 | 10 | 4.1 | 15 | 15 | 15 |

Additional Resources and Comments