Smox, CPTAC-3385 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-3385: View additional ISLYSK data

10 20 30 40 50
MQSCESSGDS ADDPLSRGLR RRGQPRVVVI GAGLAGLAAA RALLEQGFTD
60 70 80 90 100
VTVLEASSHI GGRVQSVRLG DTTFELGATW IHGSHGNPIY QLAEANGLLE
110 120 130 140 150
ETTDGERSVG RISLYSKNGV ACYLTNRGCR IPKDVVEEFS DLYNEVYNMT
160 170 180 190 200
QEFFRHGKPV NAESQNSVGV FTREKVRNRI RDDPDDTEAT KRLKLAMIQQ
210 220 230 240 250
YLKVESCESS SHSIDEVSLS AFGEWTEIPG AHHIIPSGFM RVVELLAEGI
260 270 280 290 300
PPHVIQLGKP VRCIHWDQAS AHPRGPEIEP RGEGDHNHDT GEGGQSGENP
310 320 330 340 350
QQGRWDEDEP WPVVVECEDC EVIPADHVIV TVSLGVLKRQ YTSFFRPCLP
360 370 380 390 400
TEKVAAIHRL GIGTTDKIFL EFEEPFWGPE CNSLQFVWED EAESCTLTYP
410 420 430 440 450
PELWYRKICG FDVLYPPERY GHVLSGWICG EEALVMERCD DEAVAEICTE
460 470 480 490 500
MLRQFTGNPN IPKPRRILRS AWGSNPYFRG SYSYTQVGSS GADVEKLAKP
510 520 530 540 550
LPYTESSKTA PMQVLFSGEA THRKYYSTTH GALLSGQREA ARLIEMYRDL
555
FQQGP

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3385 Collapse assay details

Data source: Panorama

Official Gene Symbol

Smox

Peptide Sequence

ISLYSK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

112

Peptide End

117

CPTAC ID

non-CPTAC-3385

Peptide Molecular Mass

709.4010

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (1+) | 3.9 | 5.5 | 3.4 | 14 | 12.3 | 4.2 | 14.5 | 13.5 | 5.4 | 15 | 15 | 15 | | y4 (1+) | 6.7 | 3.9 | 5 | 15.9 | 7.5 | 5.9 | 17.3 | 8.5 | 7.7 | 15 | 15 | 15 | | y3 (1+) | 8.1 | 4.4 | 4.8 | 13.2 | 9.3 | 6.8 | 15.5 | 10.3 | 8.3 | 15 | 15 | 15 | | y2 (1+) | 8.8 | 4.9 | 5.3 | 15.4 | 11.3 | 5.4 | 17.7 | 12.3 | 7.6 | 15 | 15 | 15 | | b2 (1+) | 5.3 | 5.4 | 7 | 14.4 | 8.2 | 6.9 | 15.3 | 9.8 | 9.8 | 15 | 15 | 15 | | sum | 3.3 | 2.7 | 2.6 | 14 | 9.4 | 2.5 | 14.4 | 9.8 | 3.6 | 15 | 15 | 15 |

Additional Resources and Comments