Alpl, CPTAC-3492 - CPTAC Assay Portal (original) (raw)
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Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| Alpl | Akp-2, Akp2 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 524 | 57514 |
| Protein Name |
|---|
| Alkaline phosphatase, tissue-nonspecific isozyme |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MISPFLVLAI | GTCLTNSFVP | EKERDPSYWR | QQAQETLKNA | LKLQKLNTNV |
| 60 | 70 | 80 | 90 | 100 |
| AKNVIMFLGD | GMGVSTVTAA | RILKGQLHHN | TGEETRLEMD | KFPFVALSKT |
| 110 | 120 | 130 | 140 | 150 |
| YNTNAQVPDS | AGTATAYLCG | VKANEGTVGV | SAATERTRCN | TTQGNEVTSI |
| 160 | 170 | 180 | 190 | 200 |
| LRWAKDAGKS | VGIVTTTRVN | HATPSAAYAH | SADRDWYSDN | EMPPEALSQG |
| 210 | 220 | 230 | 240 | 250 |
| CKDIAYQLMH | NIKDIDVIMG | GGRKYMYPKN | RTDVEYELDE | KARGTRLDGL |
| 260 | 270 | 280 | 290 | 300 |
| DLISIWKSFK | PRHKHSHYVW | NRTELLALDP | SRVDYLLGLF | EPGDMQYELN |
| 310 | 320 | 330 | 340 | 350 |
| RNNLTDPSLS | EMVEVALRIL | TKNLKGFFLL | VEGGRIDHGH | HEGKAKQALH |
| 360 | 370 | 380 | 390 | 400 |
| EAVEMDQAIG | KAGAMTSQKD | TLTVVTADHS | HVFTFGGYTP | RGNSIFGLAP |
| 410 | 420 | 430 | 440 | 450 |
| MVSDTDKKPF | TAILYGNGPG | YKVVDGEREN | VSMVDYAHNN | YQAQSAVPLR |
| 460 | 470 | 480 | 490 | 500 |
| HETHGGEDVA | VFAKGPMAHL | LHGVHEQNYI | PHVMAYASCI | GANLDHCAWA |
| 510 | 520 | 524 | ||
| GSGSAPSPGA | LLLPLAVLSL | RTLF |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for non-CPTAC-3492 Collapse assay details
Data source: Panorama
Official Gene Symbol
Alpl
Peptide Sequence
ANEGTVGVSAATER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
123
Peptide End
136
CPTAC ID
non-CPTAC-3492
Peptide Molecular Mass
1,360.6583
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 36.8 | 10.8 | 18.6 | 41.8 | 16.6 | 21.3 | 55.7 | 19.8 | 28.3 | 15 | 15 | 15 |
| y6 (1+) | 14.1 | 12.1 | 10.8 | 16.5 | 10.5 | 12.6 | 21.7 | 16 | 16.6 | 15 | 15 | 15 |
| y8 (1+) | 10.7 | 8 | 5.4 | 12.3 | 8.6 | 5.9 | 16.3 | 11.7 | 8 | 15 | 15 | 15 |
| y9 (1+) | 22.1 | 8.4 | 8.9 | 25.4 | 9.4 | 8.3 | 33.7 | 12.6 | 12.2 | 15 | 15 | 15 |
| y11 (1+) | 22.2 | 9.9 | 6.6 | 27.8 | 9.7 | 7.9 | 35.6 | 13.9 | 10.3 | 15 | 15 | 15 |
| sum | 6.1 | 3.2 | 4.2 | 9.8 | 4.5 | 5.1 | 11.5 | 5.5 | 6.6 | 15 | 15 | 15 |