Scarf2, CPTAC-3498 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-3498: View additional SVLPAATVR data

10 20 30 40 50
MEGAGSRGAG PARRQGARGL GLLLLLWLLP GLAAPQDLNP RGRNVCRTPG
60 70 80 90 100
SQVLTCCAGW RQLGDECGIA VCEGNSTCSE NEVCVRPGEC RCRHGYFGAN
110 120 130 140 150
CDTKCPRQFW GPDCKERCSC HPHGQCEDVT GQCTCHARRW GARCEHACQC
160 170 180 190 200
QHGTCHPRSG ACRCEPGWWG AQCASACYCS ATSRCDPQTG ACLCHVGWWG
210 220 230 240 250
RSCNNQCACN SSPCEQQSGR CQCRERMFGA RCDRYCQCSH GRCHPVDGTC
260 270 280 290 300
ACDPGYRGKY CREPCPAGFY GPGCRRRCGQ CKGQQPCTVV EGRCLTCEPG
310 320 330 340 350
WNGTKCDQPC ATGFYGEGCG HRCPPCRDGH ACNHVTGKCT HCNAGWIGDR
360 370 380 390 400
CETKCSNGTY GEDCAFVCSD CGSGHCDFQS GRCLCSPGVH GPHCNVTCPA
410 420 430 440 450
GLHGVDCAQA CSCHEESCDP VTGACHLETN QRKGVMGAGA LLTLLLGLLL
460 470 480 490 500
SLLGCCCACR GKDSARRELT LGRKKAPQRF CGSFSRISMK LPRIPLRRQK
510 520 530 540 550
LPKVVVAHHD LDNTLNCSFL DPPSGLEQPS PSWSSRASFS SFDTTDEGPV
560 570 580 590 600
YCVPHEEATA DSRDLEATAA LTEVAAVSLE PTGTSTPGEE AAVLPASSDS
610 620 630 640 650
ERSASSVEGP SGALYARVAR REARPARTRN EAGGLSLSPS PERRKPPPPD
660 670 680 690 700
PATKPKVSWI HGKHSAAAAA PSPPPAGRKA APSPSGRKRT PSNSSVQPPG
710 720 730 740 750
LTEEAPGPAS PTPPRARARG RGLGLSEPTD AGGPPRSAPE AASMLAAELR
760 770 780 790 800
DKTRSLGRAE KPPPPQKAKR SVLPAATVRT ASASEASGSE KAAASAPAPE
810 820 830 833
TPRKKTPIQK PPRKKSREAA GEPSRAGTAP GAS

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3498 Collapse assay details

Data source: Panorama

Official Gene Symbol

Scarf2

Peptide Sequence

SVLPAATVR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

771

Peptide End

779

CPTAC ID

non-CPTAC-3498

Peptide Molecular Mass

912.5393

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 16.8 | 7.8 | 5.9 | 17.6 | 8.1 | 5.4 | 24.3 | 11.2 | 8 | 15 | 15 | 15 | | y6 (1+) | 7.8 | 3.6 | 3.6 | 6.6 | 5.6 | 4.4 | 10.2 | 6.7 | 5.7 | 15 | 15 | 15 | | y5 (1+) | 24 | 12.1 | 11.6 | 27.2 | 13.8 | 10.5 | 36.3 | 18.4 | 15.6 | 15 | 15 | 15 | | y6 (2+) | 10.1 | 5.9 | 3.6 | 12.5 | 5.8 | 3.7 | 16.1 | 8.3 | 5.2 | 15 | 15 | 15 | | b3 (1+) | 12.7 | 5.3 | 5.8 | 11.5 | 6.4 | 6.9 | 17.1 | 8.3 | 9 | 15 | 15 | 15 | | sum | 4.4 | 2.2 | 2.2 | 6.6 | 3.5 | 3.3 | 7.9 | 4.1 | 4 | 15 | 15 | 15 |

Additional Resources and Comments