Pycard, CPTAC-3636 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| Pycard | Asc |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 193 | 21459 |
| Protein Name |
|---|
| Apoptosis-associated speck-like protein containing a CARD |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
non-CPTAC-3636: View additional TGHFVDQHR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MGRARDAILD | ALENLSGDEL | KKFKMKLLTV | QLREGYGRIP | RGALLQMDAI |
| 60 | 70 | 80 | 90 | 100 |
| DLTDKLVSYY | LESYGLELTM | TVLRDMGLQE | LAEQLQTTKE | ESGAVAAAAS |
| 110 | 120 | 130 | 140 | 150 |
| VPAQSTARTG | HFVDQHRQAL | IARVTEVDGV | LDALHGSVLT | EGQYQAVRAE |
| 160 | 170 | 180 | 190 | 193 |
| TTSQDKMRKL | FSFVPSWNLT | CKDSLLQALK | EIHPYLVMDL | EQS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for non-CPTAC-3636 Collapse assay details
Data source: Panorama
Official Gene Symbol
Pycard
Peptide Sequence
TGHFVDQHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
109
Peptide End
117
CPTAC ID
non-CPTAC-3636
Peptide Molecular Mass
1,095.5210
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 42.7 | 35.5 | 23.5 | 54.5 | 41.6 | 32.7 | 69.2 | 54.7 | 40.3 | 15 | 15 | 15 |
| y7 (3+) | 10.4 | 9.9 | 5.4 | 11.8 | 10.1 | 6.2 | 15.7 | 14.1 | 8.2 | 15 | 15 | 15 |
| y3 (2+) | 22.3 | 12.1 | 8.5 | 21.2 | 16.3 | 10.1 | 30.8 | 20.3 | 13.2 | 15 | 15 | 15 |
| y2 (1+) | 10.6 | 8.1 | 11.2 | 18.3 | 10.6 | 11.5 | 21.1 | 13.3 | 16.1 | 15 | 15 | 15 |
| y4 (1+) | 10.3 | 5.7 | 5.5 | 9.4 | 10.1 | 7.3 | 13.9 | 11.6 | 9.1 | 15 | 15 | 15 |
| sum | 6.7 | 6.1 | 2.3 | 6.3 | 8.3 | 4.2 | 9.2 | 10.3 | 4.8 | 15 | 15 | 15 |