Ces1c, CPTAC-3800 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-3800: View additional TESELLEISGK data

10 20 30 40 50
MWLHALVWAS LAVCPILGHS LLPPVVDTTQ GKVLGKYISL EGFEQPVAVF
60 70 80 90 100
LGVPFAKPPL GSLRFAPPQP AEPWSFVKNA TSYPPMCSQD AGWAKILSDM
110 120 130 140 150
FSTEKEILPL KISEDCLYLN IYSPADLTKS SQLPVMVWIH GGGLVIGGAS
160 170 180 190 200
PYNGLALSAH ENVVVVTIQY RLGIWGLFST GDEHSPGNWA HLDQLAALRW
210 220 230 240 250
VQDNIANFGG NPDSVTIFGE SSGGISVSVL VLSPLGKDLF HRAISESGVV
260 270 280 290 300
INTNVGKKNI QAVNEIIATL SQCNDTSSAA MVQCLRQKTE SELLEISGKL
310 320 330 340 350
VQYNISLSTM IDGVVLPKAP EEILAEKSFN TVPYIVGFNK QEFGWIIPMM
360 370 380 390 400
LQNLLPEGKM NEETASLLLR RFHSELNISE SMIPAVIEQY LRGVDDPAKK
410 420 430 440 450
SELILDMFGD IFFGIPAVLL SRSLRDAGVS TYMYEFRYRP SFVSDKRPQT
460 470 480 490 500
VEGDHGDEIF FVFGAPLLKE GASEEETNLS KMVMKFWANF ARNGNPNGEG
510 520 530 540 550
LPHWPEYDEQ EGYLQIGATT QQAQRLKAEE VAFWTELLAK NPPETDPTEH
554
TEHK

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3800 Collapse assay details

Data source: Panorama

Official Gene Symbol

Ces1c

Peptide Sequence

TESELLEISGK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

289

Peptide End

299

CPTAC ID

non-CPTAC-3800

Peptide Molecular Mass

1,204.6187

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b2 (1+) | 3.9 | 3.4 | 2.6 | 6 | 10.1 | 8.9 | 7.2 | 10.7 | 9.3 | 15 | 15 | 15 | | y3 (1+) | 6.8 | 4.3 | 2 | 13.5 | 10.6 | 9.4 | 15.1 | 11.4 | 9.6 | 15 | 15 | 15 | | y5 (1+) | 16.6 | 2.8 | 3.4 | 17.7 | 8.9 | 11.5 | 24.3 | 9.3 | 12 | 15 | 15 | 15 | | y6 (1+) | 19 | 6.3 | 3.5 | 19.4 | 10.8 | 11.3 | 27.2 | 12.5 | 11.8 | 15 | 15 | 15 | | y9 (1+) | 4.5 | 3.7 | 2.8 | 6.5 | 8 | 8.4 | 7.9 | 8.8 | 8.9 | 15 | 15 | 15 | | sum | 4.7 | 1.9 | 1.7 | 6 | 8.7 | 8.9 | 7.6 | 8.9 | 9.1 | 15 | 15 | 15 |

Additional Resources and Comments