Cd55, CPTAC-3972 - CPTAC Assay Portal (original) (raw)
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Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| Cd55 | Cd55a, Daf, Daf1 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 390 | 42618 |
| Protein Name |
|---|
| Complement decay-accelerating factor, GPI-anchored |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
non-CPTAC-3972: View additional VSATQHVPVTK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MIRGRAPRTR | PSPPPPLLPL | LSLSLLLLSP | TVRGDCGPPP | DIPNARPILG |
| 60 | 70 | 80 | 90 | 100 |
| RHSKFAEQSK | VAYSCNNGFK | QVPDKSNIVV | CLENGQWSSH | ETFCEKSCVA |
| 110 | 120 | 130 | 140 | 150 |
| PERLSFASLK | KEYLNMNFFP | VGTIVEYECR | PGFRKQPPLP | GKATCLEDLV |
| 160 | 170 | 180 | 190 | 200 |
| WSPVAQFCKK | KSCPNPKDLD | NGHINIPTGI | LFGSEINFSC | NPGYRLVGVS |
| 210 | 220 | 230 | 240 | 250 |
| STFCSVTGNT | VDWDDEFPVC | TEIHCPEPPK | INNGIMRGES | DSYTYSQVVT |
| 260 | 270 | 280 | 290 | 300 |
| YSCDKGFILV | GNASIYCTVS | KSDVGQWSSP | PPRCIEKSKV | PTKKPTINVP |
| 310 | 320 | 330 | 340 | 350 |
| STGTPSTPQK | PTTESVPNPG | DQPTPQKPST | VKVSATQHVP | VTKTTVRHPI |
| 360 | 370 | 380 | 390 | |
| RTSTDKGEPN | TGGDRYIYGH | TCLITLTVLH | VMLSLIGYLT |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for non-CPTAC-3972 Collapse assay details
Data source: Panorama
Official Gene Symbol
Cd55
Peptide Sequence
VSATQHVPVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
333
Peptide End
343
CPTAC ID
non-CPTAC-3972
Peptide Molecular Mass
1,165.6455
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (2+) | 23.6 | 9.1 | 5.9 | 31.4 | 12.9 | 8.1 | 39.3 | 15.8 | 10 | 15 | 15 | 15 |
| y9 (2+) | 7 | 5.1 | 3.5 | 8 | 5.6 | 6.4 | 10.6 | 7.6 | 7.3 | 15 | 15 | 15 |
| y10 (2+) | 8.8 | 3.6 | 6.3 | 17 | 10.3 | 9.7 | 19.1 | 10.9 | 11.6 | 15 | 15 | 15 |
| y2 (1+) | 8.9 | 6.5 | 5.4 | 11.3 | 9.3 | 6.5 | 14.4 | 11.3 | 8.5 | 15 | 15 | 15 |
| y4 (1+) | 11.2 | 7.5 | 3.9 | 15.6 | 10.1 | 5.4 | 19.2 | 12.6 | 6.7 | 15 | 15 | 15 |
| sum | 5.5 | 2.3 | 3.8 | 12.6 | 7.1 | 7.4 | 13.7 | 7.5 | 8.3 | 15 | 15 | 15 |