Ptprg, CPTAC-4056 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-4056: View additional AYIATQGPLK data

10 20 30 40 50
MRRLLEPCWW ILFLKITSSV LHYVVCFPAL TEGYVGTLQE SRQDSSVQIR
60 70 80 90 100
RRKASGDPYW AYSGAYGPEH WVTSSVSCGG SHQSPIDILD HHARVGDEYQ
110 120 130 140 150
ELQLDGFDNE SSNKTWMKNT GKTVAILLKD DYFVSGAGLP GRFKAEKVEF
160 170 180 190 200
HWGHSNGSAG SEHSVNGRRF PVEMQIFFYN PDDFDSFQTA ISENRIIGAM
210 220 230 240 250
AIFFQVSPRD NSALDPIIHG LKGVVHHEKE TFLDPFILRD LLPASLGSYY
260 270 280 290 300
RYTGSLTTPP CSEIVEWIVF RRPVPISYHQ LEAFYSIFTT EQQDHVKSVE
310 320 330 340 350
YLRNNFRPQQ ALNDRVVSKS AVRDAWNHDL ADFLDNPLGT EASKVCSSPP
360 370 380 390 400
IHMKVQPLNQ TALQVSWSQP ETIYHPPIMN YMISYSWTKN EDEKEKTFTK
410 420 430 440 450
DSDKDLKATI SHVSPDSLYL FRVQAVCRND MRSDFSQTML FQANTTRIFQ
460 470 480 490 500
GTRIVKTGVP TASPASSADM APISSGSSTW TSSGIPFSFV SMATGMGPSS
510 520 530 540 550
SGSQATVASV VTSTLLAGLG FGGGGISSFP STVWPTRLPT ASAASKQAGR
560 570 580 590 600
TVLATTEALA SPGPDVHSAP SKDSEGTEEG EKEEKSESED GEREHEEEEK
610 620 630 640 650
DSEKKEKSEA THTAAESDRT APAPTPSSPH RTAAEGGHQT IPGRRQDHSA
660 670 680 690 700
PATDQPGHVA PDLDPLVDTA TQVPPTATEE HYSGSDPRRP EMPSKKPMSR
710 720 730 740 750
GDRFSEDSKF ITVNPAEKNT SGMLSRPSPG RMEWIIPLIV VSALTFVCLV
760 770 780 790 800
LLIAVLVYWR GCNKIKSKGF PRRSREVPSS GERGEKGSRK CFQTAHFYVE
810 820 830 840 850
DSSSPRVVPN ESVPIIPIPD DMEAIPVKQF GKHIGELYSN SQHGFSEDFE
860 870 880 890 900
EVQRCTADMN ITAEHSNHPD NKHKNRYINI LAYDHSRVKL RPLPGKDSKH
910 920 930 940 950
SDYINANYVD GYNKAKAYIA TQGPLKSTFE DFWRMIWEQN TGIIIMITNL
960 970 980 990 1000
VEKGRRKCDQ YWPTENTEEY GNIIVTLKST KVHACYTVRR LSVRNTKVKK
1010 1020 1030 1040 1050
GQKGNPKGRQ NERTVIQYHY TQWPDMGVPE YALPVLTFVR RSSAARMPDM
1060 1070 1080 1090 1100
GPVLVHCSAG VGRTGTYIVI DSMLQQIKDK STVNVLGFLK HIRTQRNYLV
1110 1120 1130 1140 1150
QTEEQYIFIH DALLEAILGK ETAVSSSQLH SYVNSILIPG VGGKTRLEKQ
1160 1170 1180 1190 1200
FKLITQCNAK YVECFSAQKE CNKEKNRNSS VVPAERARVG LAPLPGMKGT
1210 1220 1230 1240 1250
DYINASYIMG YYRSNEFIIT QHPLPHTTKD FWRMIWDHNA QIIVMLPDNQ
1260 1270 1280 1290 1300
SLAEDEFVYW PSREESMNCE AFTVTLISKD RLCLSNEEQI IIHDFILEAT
1310 1320 1330 1340 1350
QDDYVLEVRH FQCPKWPNPD APISSTFELI NVIKEEALTR DGPTIVHDEY
1360 1370 1380 1390 1400
GAVSAGMLCA LTTLSQQLEN ENAVDVFQVA KMINLMRPGV FTDIEQYQFV
1410 1420 1430 1440 1442
YKAMLSLIST KENGNGPMTG DKNGAVLTAE ESDPAESMES LV

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-4056 Collapse assay details

Data source: Panorama

Official Gene Symbol

Ptprg

Peptide Sequence

AYIATQGPLK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

917

Peptide End

926

CPTAC ID

non-CPTAC-4056

Peptide Molecular Mass

1,060.5917

Species

Mus musculus (Mouse)

Assay Type

Direct MRM

Matrix

Plasma

Submitting Laboratory

UVic-Genome BC Proteomics Centre

Submitting Lab PI

Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6490/6495 QQQ

Internal Standard

synthetic peptide

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

1290 LC (Agilent)

Column Packing

Zorbax Eclipse Plus C18, 1.8 µm

Column Dimensions

2.1 x 150 mm

Flow Rate

400 µL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b3 (1+) | 10.2 | 6.4 | 6.3 | 11.9 | 7.1 | 5.7 | 15.7 | 9.6 | 8.5 | 15 | 15 | 15 | | b2 (1+) | 6.8 | 5.7 | 4.5 | 7.3 | 6.8 | 7 | 10 | 8.9 | 8.3 | 15 | 15 | 15 | | y4 (1+) | 15.7 | 7.2 | 7.1 | 22.2 | 6.9 | 7.8 | 27.2 | 10 | 10.5 | 15 | 15 | 15 | | y7 (1+) | 5.5 | 8.5 | 4.2 | 12.4 | 9.1 | 8.3 | 13.6 | 12.5 | 9.3 | 15 | 15 | 15 | | y8 (1+) | 6.5 | 9.8 | 5.1 | 12.4 | 9.1 | 6.3 | 14 | 13.4 | 8.1 | 15 | 15 | 15 | | sum | 2.7 | 3.2 | 2.5 | 8.1 | 4.8 | 5.5 | 8.5 | 5.8 | 6 | 15 | 15 | 15 |

Additional Resources and Comments