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Papers by claudia venegas
Iconos Revista De Ciencias Sociales, 2008
Íconos - Revista de Ciencias Sociales, 2013
Bulletin- European Association of Fish Pathologists
Piscirickeesia salmonis is the etiological agent of piscirickeesiosis, a fish disease described i... more Piscirickeesia salmonis is the etiological agent of piscirickeesiosis, a fish disease described in different locations of the world but particularly severe in maricultured salmonids in Chile. A time-course study was performed in order to observe the infection process of P. salmonis on CHSE-214 cells by confocal laser microscopy (CLM) and transmission electron microscopy with both a standard procedure (TEM) and immunogold (IG-TEM). For the CLM examination, monolayers seeded on round coverslips were studied at the following post-inoculation (pi) times: 5 min, 10 min, 15 min, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h. P. salmonis was visualized using an indirect fluorescence antibody test. In TEM and IG-TEM, monolayers grown in 25 mL culture flasks were infected and later fixed at the following pi times: 15 min, 1 h, 4 h, 6 h, 24 h, 45 h and 8 days. Results showed that P. salmonis was found aaached to the plasma membrane as early as 5 min pi. The microorganisms remained on the cell surface ...
Aquaculture and the Environment - A Shared Destiny, 2011
... and Implemented Actions to Reconstruct a More Sustainable Aquaculture Industry Pablo Ibieta1,... more ... and Implemented Actions to Reconstruct a More Sustainable Aquaculture Industry Pablo Ibieta1, Valentina Tapia1, Claudia Venegas1, Mary Hausdorf1 and Harald Takle1,2 1AVS Chile SA, Puerto Varas 2Nofima, Ås 1Chile 2Norway 1. Introduction ...
Journal of Fish Diseases, 2006
In the last 9 years, epizootics of an icterus condition has affected coho salmon, Oncorhynchus ki... more In the last 9 years, epizootics of an icterus condition has affected coho salmon, Oncorhynchus kisutch (Walbaum), reared in seawater cages in southern regions of Chile. At necropsy, fish from field cases exhibited signs of jaundice accompanied by pale light-brown livers and dark spleens. Histopathological and haematological results indicated that these fish presented haemolytic anaemia. After microbiological examination no bacterial or viral agents could be identified as aetiological agents of this disease. In an infectivity trial, coho salmon, Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum), were inoculated intraperitoneally with a filtrate of an organ homogenate (0.45 lm) from a diseased coho salmon and held for 60 days in tanks supplied with fresh water. The disease was only reproduced in coho salmon in which mortalities, beginning at day 23 postinoculation (p.i.), reached a cumulative value of 24% at day 27 p.i. This condition was transmitted to non-inoculated cohabiting coho salmon suggesting that it is a waterborne disease. Thus, this icteric condition is caused by an infectious form of haemolytic anaemia, probably of viral aetiology, and coho salmon are more susceptible than either Atlantic salmon or rainbow trout.
Fish Pathology, 1999
Penaeid acute viremia (PAV) caused by penaeid rod-shaped DNA virus (PRDV: one of white spot bacul... more Penaeid acute viremia (PAV) caused by penaeid rod-shaped DNA virus (PRDV: one of white spot baculovirus complex) is the most serious disease affecting kuruma prawn (Penaeus japonicus) culture in Japan. In this study an experimental challenge was carried out in different larval (nauplius, zoea and mysis) and postlarval (PL 1, 6, 9 and 11-12) stages of kuruma prawn in order to elucidate the difference in susceptibility to PRDV among different developmental stages of the host. The PRDV challenge was done by immersion in all the tested groups. No infections were recorded in the larval stages and PL1. The PL6 first showed mortality due to PAV on 10th day post-inoculation. The PL9 exhibited the onset of PAV on 5th day post-inoculation.
Diseases of Aquatic Organisms, 2000
BMC Genomics, 2012
Background: Aquaculture of piscivorous fish is in continual expansion resulting in a global requi... more Background: Aquaculture of piscivorous fish is in continual expansion resulting in a global requirement to reduce the dependence on wild caught fish for generation of fishmeal and fish oil. Plant proteins represent a suitable protein alternative to fish meal and are increasingly being used in fish feed. In this study, we examined the transcriptional response of Atlantic salmon (Salmo salar) to a high marine protein (MP) or low fishmeal, higher plant protein replacement diet (PP), formulated to the same nutritional specification within previously determined acceptable maximum levels of individual plant feed materials.
Aquaculture Nutrition, 2014
ABSTRACT The effects on Atlantic salmon (Salmo salar L.) metabolic health of including modern pro... more ABSTRACT The effects on Atlantic salmon (Salmo salar L.) metabolic health of including modern processed land animal by-products (LAP) to a plant- and marine-based diet (50% marine and 50% plant ingredients) were investigated. Three experimental diets were made with systematic replacements of both marine and plant ingredients with LAP as a source of protein (poultry meal and porcine blood meal) and fat (poultry oil) to fit a two-way factorial design. A 16-week feeding trial was performed with postsmolts in seawater (initial weight 372 g). The diet with both protein and lipids from LAP reduced liver triacylglycerols more than fourfold compared to the diet without LAP. This was confirmed by histological examinations showing reduced fatty degeneration in the liver of fish fed the high LAP diet. No severe negative effects on gut or tissue health were seen by histological examinations or by measuring genetic markers with qPCR, although a trend in the histological results indicated an improved gut health by including LAP in the diets. Clinical analyses of plasma and lipoprotein fractions showed no differences between dietary groups.
Iconos Revista De Ciencias Sociales, 2008
Íconos - Revista de Ciencias Sociales, 2013
Bulletin- European Association of Fish Pathologists
Piscirickeesia salmonis is the etiological agent of piscirickeesiosis, a fish disease described i... more Piscirickeesia salmonis is the etiological agent of piscirickeesiosis, a fish disease described in different locations of the world but particularly severe in maricultured salmonids in Chile. A time-course study was performed in order to observe the infection process of P. salmonis on CHSE-214 cells by confocal laser microscopy (CLM) and transmission electron microscopy with both a standard procedure (TEM) and immunogold (IG-TEM). For the CLM examination, monolayers seeded on round coverslips were studied at the following post-inoculation (pi) times: 5 min, 10 min, 15 min, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h. P. salmonis was visualized using an indirect fluorescence antibody test. In TEM and IG-TEM, monolayers grown in 25 mL culture flasks were infected and later fixed at the following pi times: 15 min, 1 h, 4 h, 6 h, 24 h, 45 h and 8 days. Results showed that P. salmonis was found aaached to the plasma membrane as early as 5 min pi. The microorganisms remained on the cell surface ...
Aquaculture and the Environment - A Shared Destiny, 2011
... and Implemented Actions to Reconstruct a More Sustainable Aquaculture Industry Pablo Ibieta1,... more ... and Implemented Actions to Reconstruct a More Sustainable Aquaculture Industry Pablo Ibieta1, Valentina Tapia1, Claudia Venegas1, Mary Hausdorf1 and Harald Takle1,2 1AVS Chile SA, Puerto Varas 2Nofima, Ås 1Chile 2Norway 1. Introduction ...
Journal of Fish Diseases, 2006
In the last 9 years, epizootics of an icterus condition has affected coho salmon, Oncorhynchus ki... more In the last 9 years, epizootics of an icterus condition has affected coho salmon, Oncorhynchus kisutch (Walbaum), reared in seawater cages in southern regions of Chile. At necropsy, fish from field cases exhibited signs of jaundice accompanied by pale light-brown livers and dark spleens. Histopathological and haematological results indicated that these fish presented haemolytic anaemia. After microbiological examination no bacterial or viral agents could be identified as aetiological agents of this disease. In an infectivity trial, coho salmon, Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum), were inoculated intraperitoneally with a filtrate of an organ homogenate (0.45 lm) from a diseased coho salmon and held for 60 days in tanks supplied with fresh water. The disease was only reproduced in coho salmon in which mortalities, beginning at day 23 postinoculation (p.i.), reached a cumulative value of 24% at day 27 p.i. This condition was transmitted to non-inoculated cohabiting coho salmon suggesting that it is a waterborne disease. Thus, this icteric condition is caused by an infectious form of haemolytic anaemia, probably of viral aetiology, and coho salmon are more susceptible than either Atlantic salmon or rainbow trout.
Fish Pathology, 1999
Penaeid acute viremia (PAV) caused by penaeid rod-shaped DNA virus (PRDV: one of white spot bacul... more Penaeid acute viremia (PAV) caused by penaeid rod-shaped DNA virus (PRDV: one of white spot baculovirus complex) is the most serious disease affecting kuruma prawn (Penaeus japonicus) culture in Japan. In this study an experimental challenge was carried out in different larval (nauplius, zoea and mysis) and postlarval (PL 1, 6, 9 and 11-12) stages of kuruma prawn in order to elucidate the difference in susceptibility to PRDV among different developmental stages of the host. The PRDV challenge was done by immersion in all the tested groups. No infections were recorded in the larval stages and PL1. The PL6 first showed mortality due to PAV on 10th day post-inoculation. The PL9 exhibited the onset of PAV on 5th day post-inoculation.
Diseases of Aquatic Organisms, 2000
BMC Genomics, 2012
Background: Aquaculture of piscivorous fish is in continual expansion resulting in a global requi... more Background: Aquaculture of piscivorous fish is in continual expansion resulting in a global requirement to reduce the dependence on wild caught fish for generation of fishmeal and fish oil. Plant proteins represent a suitable protein alternative to fish meal and are increasingly being used in fish feed. In this study, we examined the transcriptional response of Atlantic salmon (Salmo salar) to a high marine protein (MP) or low fishmeal, higher plant protein replacement diet (PP), formulated to the same nutritional specification within previously determined acceptable maximum levels of individual plant feed materials.
Aquaculture Nutrition, 2014
ABSTRACT The effects on Atlantic salmon (Salmo salar L.) metabolic health of including modern pro... more ABSTRACT The effects on Atlantic salmon (Salmo salar L.) metabolic health of including modern processed land animal by-products (LAP) to a plant- and marine-based diet (50% marine and 50% plant ingredients) were investigated. Three experimental diets were made with systematic replacements of both marine and plant ingredients with LAP as a source of protein (poultry meal and porcine blood meal) and fat (poultry oil) to fit a two-way factorial design. A 16-week feeding trial was performed with postsmolts in seawater (initial weight 372 g). The diet with both protein and lipids from LAP reduced liver triacylglycerols more than fourfold compared to the diet without LAP. This was confirmed by histological examinations showing reduced fatty degeneration in the liver of fish fed the high LAP diet. No severe negative effects on gut or tissue health were seen by histological examinations or by measuring genetic markers with qPCR, although a trend in the histological results indicated an improved gut health by including LAP in the diets. Clinical analyses of plasma and lipoprotein fractions showed no differences between dietary groups.