cemile koca | Ankara Yildirim Beyazit University (original) (raw)

Papers by cemile koca

Research paper thumbnail of Comparison of serum levels of IL-6, IL-8, TNF-α, C reactive protein and heat shock protein 70 in patients with active or inactive Behçet’s disease

Turkish Journal of Medical Sciences

The aim of this study was to elucidate serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 in pati... more The aim of this study was to elucidate serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 in patients with active or inactive Behçet's disease. Materials and methods: The study included 50 patients who met the International Study Group criteria for Behçet's disease. Of these, 26 had active disease and 24 had inactive disease. The control group was comprised of 25 age-and sexmatched healthy participants. Serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 were measured. Results: In patients with active disease, significantly higher mean serum levels of IL-6, IL-8, TNF-α, and CRP were found compared to patients with inactive disease or in controls (overall, P < 0.05). In patients with active disease, the mean serum level of HSP 70 was significantly higher than that of the control group (P = 0.02). In patients with inactive disease it was higher compared to the controls but with a significance level close to 0.05 (P = 0.044). However, HSP 70 levels did not differ significantly between patients with active disease and patients with inactive disease (P = 0.93). Conclusion: The results of our study suggest that serum IL-6, IL-8, TNF-α, and CRP levels are increased in patients with active Behçet's disease. HSP 70 levels in both active and inactive disease groups were higher than in controls, though it was significant only in the active group. This suggests that HSP 70 has a role in the chronic nature of Behçet's disease, with HSP 70 expression possibly not falling to normal levels in the inactive phase of the disease.

Research paper thumbnail of Immunomodulatory role of leptin treatment in experimental sepsis caused by gram negative bacteria

Turkish Journal of Medical Sciences

To investigate the eff ect of leptin treatment on circulating infl ammatory cytokines and on tiss... more To investigate the eff ect of leptin treatment on circulating infl ammatory cytokines and on tissue damage in experimental rat model of gram-negative sepsis. Materials and methods: Adult male Wistar rats, 28 in total, were randomly divided into 4 groups (n = 7): sham, leptin, sepsis, and sepsis group treated with leptin (sepsis+leptin). Sepsis was induced by intraperitoneal (ip) injection of 2 × 10 10 CFU of Escherichia coli ATCC 25922. Leptin and sepsis+leptin groups received a single dose ip 0.1 mg/kg leptin, while sham group received 1 mL of ip saline. Rats were sacrifi ced 24 h aft er the induction of sepsis. Blood samples, lung, and kidney tissues were collected for analysis. Results: Th e sepsis group had signifi cantly higher serum TNF-α, IL-6, and endothelin levels than the sham group (P = 0.05, P < 0.001, P = 0.003, respectively). Th e sepsis+leptin group had signifi cantly lower IL-6 and endothelin levels (P = 0.001 and P = 0.020, respectively), and higher lung and kidney tissue myeloperoxidase activities when compared with the sepsis group (P = 0.039, P = 0.033, respectively). Conclusion: According to our results, leptin has a profound infl uence on sepsis, and to some extent it restricts the infl ammatory events in sepsis.

Research paper thumbnail of Subclinical hypoxia of infants with intrauterine growth retardation determined by increased serum S100B protein levels

Turkish Journal of Medical Sciences

To test the hypothesis that serum S100B levels could be useful in detecting neurological damage i... more To test the hypothesis that serum S100B levels could be useful in detecting neurological damage in infants with intrauterine growth retardation (IUGR). Materials and methods: Th e study group consisted of infants with IUGR and the control group consisted of agematched healthy infants. S100B protein levels were measured aft er birth and compared between groups. Results: For this study, 43 infants with IUGR and 25 infants as a control group were recruited. Gender, gestational age, type of delivery, and maternal age of the groups were statistically insignifi cant, with the exception of the mean birth weights (2120 ± 450 g in the IUGR group and 3096 ± 570 g in the control group (P < 0.001), respectively). S100B protein levels of the IUGR infants (1.13 ± 0.54) were signifi cantly higher than those of the control group (0.45 ± 0.13) (P < 0.001). IUGR infants treated with antenatal steroids showed lower S100B levels than IUGR infants that did not receive antenatal steroid treatments (P < 0.05). Th e study group infants were divided into 2 groups, for growth retardation (GR) that was asymmetric (n = 15) and symmetric (n = 28). Th e asymmetric and symmetric GR infants' S100B levels were 1.14 ± 0.47 pg/mL and 1.21 ± 0.34 pg/mL, respectively, and no signifi cant diff erences were found between the 2 groups in terms of S100B levels (P = 0.32). Conclusion: Th e results of this study favor the opinion that there is an existing intrauterine hypoxia causing hypoxic brain tissue damage in IUGR infants, even when followed up with modern obstetrical screening protocols. Measurements of S100B may be useful in the prediction of outcome in these infants.

Research paper thumbnail of Effects of Erdosteine in Experimental Sepsis Model in Rats

Hong Kong Journal of Emergency Medicine, 2011

Objective Erdosteine is a mucolytic agent that is known to possess antioxidant effects. This stud... more Objective Erdosteine is a mucolytic agent that is known to possess antioxidant effects. This study investigated the effects of erdosteine on endothelin-1 (ET-1) levels and oxidative stress parameters superoxide dismutase (SOD) and malondialdehyde (MDA) in a rat sepsis model. Methods Four groups of Wistar albino rats (n=8 per group) were randomly allocated to the following groups: sham (group 1), sepsis (group 2), erdosteine control (group 3) and a sepsis group pretreated with erdosteine (group 4). Sepsis was induced using E. Coli ATCC 25922 inoculation. Serum ET-1, liver tissue SOD and MDA levels were determined in all groups. Results ET-1 levels were significantly higher in group 2 compared to groups 1, 3 and 4 (p<0.001, p=0.002 and p<0.001, respectively). Similarly, MDA levels in groups 1, 3 and 4 were significantly lower relative to group 2 (p<0.001, p=0.022 and p=0.010, respectively). Additionally, SOD activities in these same three groups were found to be significantly...

Research paper thumbnail of Erratum for: Association of coronary artery disease severity and disulphide/native thiol ratio

Electronic Journal of General Medicine, 2018

Research paper thumbnail of Responses of Anterior Pituitary Hormones to Fever During Community-Acquired Infections

Electronic Journal of General Medicine, 2008

INTRODUCTION Fever is a part of the acute phase response (APR) to infection and systemic inflamma... more INTRODUCTION Fever is a part of the acute phase response (APR) to infection and systemic inflammation. It is a part of a complex physiological response of the host against microorganisms or foreign non-microbial agents invading the body (1,2). The fever is induced by infammatory mediators released by immune cells activated by contacts with foreign molecules (1,2). Recent studies have demonstrated that there are bidirectional circuits between the central nervous systems.(CNS) and the immune system 3,4. The hypothalamicpituitary-adrenal (HPA) axis plays an important role between the immune

Research paper thumbnail of The Effect of Dexmedetomidine and Propofol on Oxidative Stress Parameters during Lower Extremity Surgery: A Prospective Randomized Trial

International Journal of Anesthetics and Anesthesiology, 2015

Background: This study aims to compare the impact of sedation, continuous dexmedetomidine and pro... more Background: This study aims to compare the impact of sedation, continuous dexmedetomidine and propofol infusion, to oxidative stress that occurred as a result of tourniquet-induced ischemia reperfusion (IR) during lower extremity surgery. Material & Methods: All patients were administered combined spinoepidural anesthesia; Group D received infusion of 1µg kgdexmedetomidine for 10 minutes and 0.5µg kghinfusion, Group P was administered 0.2 mg kgpropofol following bolus 2 mg kgmin-. At baseline, 20 minutes and two hours after the tourniquet was released, plasma total antioxidant status (TAS), total oxidant status (TOS), paraoxonase (PON), stimulated paraoxonase (SPON), arylesterase, ceruloplasmin, myeloperoxidase (MPO), ischemia modified albumin (IMA) and advanced oxidation protein products (AOPP) levels were analyzed. Results: In Group D, ceruloplasmin values taken post tourniquet release were lower compared to the baseline values (p = 0.02 and 0.008, respectively). As for Group P, a decrease in the TAS and TOS values was recorded two hours after the tourniquets were released (in both p = 0.008). No differences were found in other markers of oxidative stress during intra-group comparisons (p > 0.05). Conclusion: The study concluded that administration of propofol and dexmedetomidine sedation during lower extremity surgery had similar effects on oxidative stress caused by tourniquet-induced IR.

Research paper thumbnail of A novel marker in acute central serous chorioretinopathy: thiol/disulfide homeostasis

International Ophthalmology, 2017

Purpose The aim of this study was to compare dynamic thiol/disulfide homeostatic status in acute ... more Purpose The aim of this study was to compare dynamic thiol/disulfide homeostatic status in acute central serous chorioretinopathy (CSCR) patients by using a novel and automated assay determining dynamic thiol/disulfide homeostasis.MethodsFifty-one patients with acute CSCR (study group) and 65 healthy individuals (control group) were enrolled in this study. Diagnosis of acute CSCR was made clinically and using spectral-domain RTVue OCT (optical coherence tomography) (Optovue, Fremont, CA). Fluorescein angiography confirmed the diagnosis of acute CSCR in all subjects. Total thiol, native thiol, disulfide amount, and native thiol/disulfide ratio (TDR) were calculated in the blood samples.ResultsMean total thiol, native thiol, and native TDR values were lower in patients with acute CSCR (364.2 ± 14.1, 326.4 ± 13.2, 17.14 ± 1.9, respectively) than in healthy eyes (441.2 ± 16.3, 398.5 ± 16.4, 22.70 ± 2.15, respectively; mean total thiol, p = 0.017; native thiol, p = 0.011; native TDR, p = 0.031).ConclusionsTotal thiol, native thiol, and native TDR were significantly lower statistically in patients with acute CSCR when compared with healthy controls.

Research paper thumbnail of Thiol/Disulfide Homeostasis in Patients with Central Serous Chorioretinopathy

Current Eye Research, 2016

To evaluate dynamic thiol/disulfide homeostasis in patients with chronic central serous chorioret... more To evaluate dynamic thiol/disulfide homeostasis in patients with chronic central serous chorioretinopathy (cCSC). This prospective study included 34 cCSC cases and 37 healthy individuals who were age- and sex-matched. A new colorimetric method for measuring thiol/disulfide homeostasis was used. Native thiol, total thiol/disulfide levels, disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios were measured. The age and gender distributions were similar in both main groups. The mean duration of disease was 47.29 ± 24 months. Native and total thiol levels were significantly lower among the cCSC group relative to the control group (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). There was not a statistically significant difference between the cCSC and the control group in terms of disulfide levels (p = 0.492). While disulfide/native thiol and disulfide/total thiol ratios were elevated, the native thiol/total thiol ratio was decreased in the cCSC group (p = 0.017, 0.021, 0.036, respectively). Ratios obtained using plasma native thiol, total thiol, and disulfide levels differed significantly between the both groups. Disulfide/thiol ratio was significantly greater in cCSC patients relative to healthy control subjects. Our results suggest that the oxidative process is involved in the pathogenesis of the cCSC.

Research paper thumbnail of Assessment of fetal antioxidant and oxidant status during different anesthesia techniques for elective cesarean sections

Journal of Research in Medical Sciences, 2015

and more popular because of the development of anesthesia techniques and materials besides patien... more and more popular because of the development of anesthesia techniques and materials besides patients can remain conscious. In the case of cesarean section surgery, for the determination of the method of anesthesia, not only patient and doctors preferences but also fetal status is important. Although general anesthesia is known to have negative effects on the baby's Apgar scores, [4] it is unavoidable for some patients who refused regional anesthesia due to fear of the needless, emergency conditions, and medical contraindication such as coagulation disorders, aortic stenosis, and infection. [5] Although oxidative and anti-oxidative stress marker levels were shown to be higher in cesarean section Background: We aimed to investigate the effects of general, spinal and epidural anesthesia on fetal total antioxidant status (TAS) and total oxidant status (TOS), and oxidative stress index (OSI) during elective cesarean section in this study. Materials and Methods: Forty-seven parturients scheduled for elective cesarean section were randomly allocated into three groups: Group spinal (n = 15), group epidural (n = 17), and group general (n = 15), This prospective randomized study was performed in Faculty of Medicine, Turgut Ozal University, Turkey. After the baby was delivered; TAS, TOS levels, and arterial blood gases parameters were analyzed in an umbilical arterial blood sample. OSI values are calculated by a ratio of TOS to the TAS. Results: The levels of TAS and TOS in umbilical arterial blood sample were not statistically different among three. However, OSI values were significantly different among the three groups (P = 0.042). Median OSI values is 24 (interquartile range [IQR], 2-37) in group spinal, 19 (IQR, 4-44) in group epidural, and 8 (IQR, 4-36) in group general. There was no significant difference in OSI values in the comparison of group spinal with group general and group epidural, but it was significantly lower in group general when compared with group epidural with Bonferroni correction (P = 0.017). Umbilical cord arterial blood gas values (pH, PaCO 2 , PaO 2 , SaO 2 , HCO 3 , and CtO 2), glucose, lactate, and hemoglobin levels were similar in three groups. Conclusion: General anesthesia may be more favorable than epidural in those undergoing cesarean section when fetal oxidative status gains importance.

Research paper thumbnail of Antioxidant effects of curcumin in spinal cord injury in rats

Turkish Journal of Trauma and Emergency Surgery, 2011

Bu deneysel çalışma, sıçanlarda spinal kord yaralanmasında curcuminin antioksidan etki yoluyla fa... more Bu deneysel çalışma, sıçanlarda spinal kord yaralanmasında curcuminin antioksidan etki yoluyla faydasını araştırmak için yapıldı. GEREÇ VE YÖNTEM Yirmi dört adet Wistar albino sıçan 3 gruba randomize edildi. Spinal kord yaralanması ağırlık düşürme modeliyle gerçekleştirildi. Grup 1'e laminektomi ardından spinal kord yaralanması uygulandı ve herhangi bir tedavi verilmedi. Grup 2'ye laminektomi ardından spinal kord yaralanması uygulandı ve curcumin verildi (200 mg/kg/gün ağızdan). Grup 3'e laminektomi ardından spinal kord yaralanması uygulandı ve metilprednizolon verildi (30 mg/kg periton içine), 24 saat sonra tüm sıçanlardan kan örnekleri alındı, sonra serum süperoksit dismutaz (SOD) ve malondialdehit (MDA) düzeyleri belirlendi ve elde edilen sonuçlar karşılaştırıldı. BULGULAR Curcumin grubunda SOD düzeyi kontrol ve metilprednizolon grubundan daha yüksekti (p<0,001 ve p<0,012). Curcumin grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,042). Benzer şekilde metilprednizolon grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,001). SONUÇ Bu çalışmanın sonuçları curcuminin etkin biçimde oksidatif hasara karşı spinal kord dokularını koruduğunu gösterir.

Research paper thumbnail of Value of Caffeic Acid Phenethyl Ester Pretreatment in Experimental Sepsis Model in Rats

Mediators of Inflammation, 2015

Background and Aim. The aim of this study was to determine the actions of caffeic acid phenethyl ... more Background and Aim. The aim of this study was to determine the actions of caffeic acid phenethyl ester (CAPE) on the changes of endothelin-1 (ET-1) level, tumor necrosis factor- (TNF-) alpha, and oxidative stress parameters such as superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in experimental sepsis model in rats.Materials and Methods. Twenty-four rats were randomly divided into three experimental groups: sham (group 1), sepsis (group 2), and sepsis + CAPE (group 3),n= 8 each. CAPE was administered (10 µmol/kg) intraperitoneally to group 3 before sepsis induction. Serum ET-1, serum TNF-alpha, tissue SOD activity, and tissue MDA levels were measured in all groups.Results. Pretreatment with CAPE decreased ET-1, TNF-alpha, and MDA levels in sepsis induced rats. Additionally SOD activities were higher in rats pretreated with CAPE after sepsis induction.Conclusion. Our results demonstrate that CAPE may have a beneficial effect on ET and TNF-alpha levels and oxida...

Research paper thumbnail of Lipid peroxidation and scavenging enzyme levels in the liver of streptozotocin-induced diabetic rats

Indian journal of biochemistry & biophysics, 2003

In this study, alterations in the liver antioxidant enzymes status and lipid peroxidation in shor... more In this study, alterations in the liver antioxidant enzymes status and lipid peroxidation in short-term (8-weeks) and long-term (24-weeks) diabetic rats were examined. Glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) levels were significantly increased, but superoxide dismutase (SOD) activity was significantly reduced in 8-weeks diabetic rats, compared to control. Catalase (CAT) activity, however, was found unchanged. In 24-weeks diabetic rats, while GSH-Px activity was unchanged, but SOD and CAT activities and MDA levels were significantly increased, compared to control. These results suggest that diabetes-induced alterations in tissue antioxidant system may reflect a generalized increase in tissue oxidative stress. It can be concluded that lipid peroxidation and antioxidant enzyme levels are elevated in diabetic condition. Hence, diabetes mellitus, if left untreated, may increase degenerative processes due to accumulation of oxidative free radicals.

Research paper thumbnail of Effects of Different Fluids on Hymodynamic Parameters and Liver Arginase Activities in Hemorrhagic Shock

AIM: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to res... more AIM: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to research the effect of different fluid resuscitation on the liver arginase activity. MATERIALS AND METHODS: Fifty rats were divided into five groups. In group 1; massive splenic injury was untreated, in group 2 massive injury was treated with 70 ml/kg/hour Ringer Lactate solution, in groups 3,4,5 massive injury was treated with 7.5 ml/kg/hour Hydroxyethyl Starch, Hypertonic Saline and Dextran-40, respectively between 15 and 30 minutes of injury. In all groups, splenectomy was performed at the 30th minute. Hemodynamic monitoring and anesthesia were continued up to 90 minutes. Duration of follow-up was 48 hrs. After 48 hours of survival, liver tissue samples were removed and were kept in -70◦C for the analysis of arginase activities. RESULTS: Different fluid regimens were found to have no effects on hemodynamic parameters, arteriel blood gases and survival at the early phase of uncontrolled hemorrhagic shock. RL increased liver arginase activity compared with control group (p<0.02) and other groups. (p<0.05). Unlike Ringer Lactate, other groups had no statistical differences at arginase activities comparing to control group. CONCLUSIONS: Finally, depending on long term results, Ringer Lactate is more useful fluid choice in hemorrhagic shock.

Research paper thumbnail of Decreased Plasma Vitamin D Levels in Patients with Undifferentiated Spondyloarthritis and Ankylosing Spondylitis

Internal Medicine, 2013

Objective The aim of the present study was to evaluate the plasma vitamin D (vit D) levels and th... more Objective The aim of the present study was to evaluate the plasma vitamin D (vit D) levels and their association with the disease activity in patients with ankylosing spondylitis (AS) and undifferentiated spondyloarthritis (uSpA) compared with healthy populations. Methods This study included 161 spondyloarthritis patients (113 uSpA patients and 48 AS patients) attending our rheumatology outpatient clinic, along with 92 controls. Results The plasma vit D levels were 18 μg/L (8-38) in the AS group, 20 μg/L (4-92.3) in the uSpA group and 24.3 μg/L (7.2-76.8) in the control group. The plasma vit D levels of the AS patients were significantly lower than those of the patients in the control group (p=0.004). The men in the AS group had significanly lower vit D levels than those in the control group (p=0.005). On the other hand, the women in the uSpA group had significanly lower vit D levels than those in the control group (p=0.011). The vit D levels were inversely related to both erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in the AS patients (p=0.002, R=-0.428; p<0.001, R=-0.592, respectively). This correlation was not demonstrated in the uSpA patients. The vit D levels were not found to correlate with the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) levels in either the AS or uSpA patients. Conclusion 25-hydroxy-vit D deficiency is frequently observed in patients with SpAs. In this study, vit D deficiency was much more prominent in the male AS patients. On the other hand, among women, the uSpA patients exhibited much more prominent vit D deficiency than the control group subjects. The acute phase response may inversely affect the vit D levels in AS patients.

Research paper thumbnail of Association of polycystic ovary syndrome and a non-dipping blood pressure pattern in young women

Clinics, 2010

OBJECTIVE: The association between polycystic ovarian syndrome and increased cardiovascular disea... more OBJECTIVE: The association between polycystic ovarian syndrome and increased cardiovascular disease risk is still a controversial issue. In light of data documenting some common pathways or common end-points, the present study was undertaken to determine whether there is a relationship between sleep blood pressure pattern disturbances and polycystic ovarian syndrome in young women. METHOD: The daytime and nighttime ambulatory blood pressures (BPs) were determined for each subject, according to the actual waking and sleeping times recorded in their individual diaries, in this cross-sectional study. RESULTS: The study group comprised 168 women (mean age: 25.7±5.5) diagnosed with polycystic ovarian syndrome, while the control group included 52 age-and BMI-matched healthy subjects (mean age: 26.1±5.4). When nocturnal BP declines very little or not at all, with the BP falling less than 10% during sleep compared with waking values, this pattern is classified as a non-dipping BP pattern. However, the non-dipping pattern of BP changes was significantly more common in polycystic ovarian syndrome patients compared to the control group (p<0.01). The prevalence of a non-dipping BP pattern was 43.4% (73 patients) in polycystic ovarian syndrome patients and 3.9% (2 patients) in the control group. CONCLUSION: Our cross-sectional study revealed that a non-dipping BP pattern is highly prevalent in polycystic ovarian syndrome patients, even if they are young and non-obese.

Research paper thumbnail of Evaluation of Asymmetric Dimethylarginine (ADMA) Levels in Children with Growth Hormone Deficiency

Journal of Clinical Research in Pediatric Endocrinology, 2014

Ori gi nal Ar tic le ABS TRACT Ob jec ti ve: To investigate serum asymmetric dimethylarginine (AD... more Ori gi nal Ar tic le ABS TRACT Ob jec ti ve: To investigate serum asymmetric dimethylarginine (ADMA) levels in children with isolated growth hormone deficiency (GHD) and to determine the effect of GH replacement therapy on these levels. Methods: 31 patients diagnosed with isolated GHD and 29 age-and sexmatched healthy children were enrolled in the study. Height, weight and waist circumference were measured in all subjects. Fasting serum insulin-like growth factor-1 (IGF-1), IGF binding protein-3, glucose, insulin and lipid levels were evaluated. Serum ADMA levels were assessed using the enzyme-linked immunosorbent assay technique. The same evaluations were repeated on the 3 rd and 6 th months of treatment in 28 of the GHD cases. Results: There were no significant differences in ADMA levels between the patient and control groups [0.513±0.130 (0.291-0.820) μmol/L vs. 0.573±0.199 (0.241-1.049) μmol/L]. There was a positive correlation between serum ADMA and HbA1c levels in the control group. In the GHD cases, ADMA levels negatively correlated with high-density lipoprotein levels and positively correlated with low-density lipoprotein levels. There was also a significant increase in ADMA levels in patients receiving GH therapy compared to pre-treatment levels [serum ADMA level, 1.075±0.133 (0.796-1.303) μmol/L at the 3 rd month and 0.923±0.121 (0.695-1.159) μmol/L at the 6 th month of treatment]. There was a negative correlation between ADMA levels and homeostasis model assessment of insulin resistance values at the 6 th month evaluation. There were no relationships between ADMA levels and age, sex, or pubertal state either before or during the treatment. Conclusion: Serum ADMA levels were found to be similar in patients with GHD and in healthy children. However, serum ADMA levels showed a significant increase in GHD patients following GH replacement therapy.

Research paper thumbnail of The Effect of Purified Micronized Flavonoid Fraction on the Healing of Anastomoses in the Colon in Rats

Surgery Today, 2006

collagenolysis, deposition of new collagen, and maturation. 1 There is a complex interaction of m... more collagenolysis, deposition of new collagen, and maturation. 1 There is a complex interaction of many factors and collagen turnover. 2 Anastomotic leakage is a major complication of surgery of the digestive tract. 3-5 Morbidity and mortality rates because of clinically evident leakage are still high, despite advances in operative techniques and suture materials. 4 The reported incidences of leakage of colorectal anastomoses vary greatly. Using contrast studies, leakage was detected in up to 50% of patients who underwent an anterior resection. 6,7 Clinically, apparent anastomotic leakage ranges from 3.4% to 8%. 8 Anastomotic leakage increases the hospital length of stay twofold and perioperative morbidity threefold. 9 Flavonoids are a chemically active heterogeneous group of plant extracts naturally occurring as food chemicals with considerable structural, biochemical, and pharmacological diversity. 10,11 The mechanisms of flavonoid action are multifactorial. Flavonoids have distinct effects on the function of immune and inflammatory cells both in vivo and in vitro: the release of inflammatory mediators and cytotoxic enzymes, the formation of oxygen radical species, and the homophilic and/or heterophilic adhesiveness of inflammatory cells. Flavonoids also have a beneficial action on collagen sythesis. 10 With the aforementioned effects of flavonoids, the objective of this study was to investigate the effects of clinically used, micronized, purified, flavonoid fractions (Daflon, 500 mg; Servier, Turkey) on the healing of anastomoses in the colon of rats. Methods The Ethics Committee of Fatih University Medical School approved this experimental protocol. The study was performed in accordance with the internationally accepted principles for laboratory animals.

Research paper thumbnail of Is IL-33 useful to detect early stage of renal failure?

Renal Failure, 2013

IL-33 is a proinflammatory cytokine that is a member of IL-1 family. Previously the effect of IL-... more IL-33 is a proinflammatory cytokine that is a member of IL-1 family. Previously the effect of IL-33 on kidney injury is showed in animal models. In this study, we searched if we can use IL-33 to show the early stage of kidney injury in diabetic patients. Three groups are identified: 26 patients in Group 1: Healthy group, that do not have any chronic diseases and not taking any medication; 42 patients in Group 2: DM (diabetes mellitus) group without any known kidney disease and with normal kidney functions; 32 patients in Group 3: DM + MA (microalbuminuria) group that are assumed to have nephropathy. IL-33 level of DM patient group is greater than healthy group; also IL-33 level of DM + MA patient group is greater than healthy group; but there is not any difference between DM and DM + MA group. The increase in IL-33 levels in diabetic nephropathy is not associated with kidney injury but the increase could be resulting because of diabetes. So IL-33 cannot be used in early recognition of diabetic nephropathy.

Research paper thumbnail of Nitric Oxide Affects Serum Ferritin Levels in Children with Iron Deficiency

Pediatric Hematology-Oncology, 2007

In iron deficiency, serum levels of ferritin decrease. The lack of iron has been thought to be th... more In iron deficiency, serum levels of ferritin decrease. The lack of iron has been thought to be the main factor in this decrease, but another potential factor is nitric oxide, which has been shown to affect ferritin metabolism in vitro. The aim of this study was therefore to evaluate in children with iron deficiency the relation of serum ferritin, nitric oxide degradation products (nitrate and nitrite), and endothelin-1, a protein closely related to nitric oxide function. A total of 80 children were included in the study (39 with iron deficiency, 41 controls). Serum levels of ferritin, nitrate, nitrite, and endothelin-1 were measured in all participants. In children with iron deficiency, nitrate and nitrite levels were significantly higher (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .009 and .01, respectively). Also, serum ferritin was negatively correlated with serum levels of nitrate and nitrite (p = .034, r = -.254 for nitrate and p = .01, r = -.593 for nitrite). No statistical relationship was found between serum ferritin and endothelin-1.

Research paper thumbnail of Comparison of serum levels of IL-6, IL-8, TNF-α, C reactive protein and heat shock protein 70 in patients with active or inactive Behçet’s disease

Turkish Journal of Medical Sciences

The aim of this study was to elucidate serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 in pati... more The aim of this study was to elucidate serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 in patients with active or inactive Behçet's disease. Materials and methods: The study included 50 patients who met the International Study Group criteria for Behçet's disease. Of these, 26 had active disease and 24 had inactive disease. The control group was comprised of 25 age-and sexmatched healthy participants. Serum levels of IL-6, IL-8, TNF-α, CRP, and HSP 70 were measured. Results: In patients with active disease, significantly higher mean serum levels of IL-6, IL-8, TNF-α, and CRP were found compared to patients with inactive disease or in controls (overall, P < 0.05). In patients with active disease, the mean serum level of HSP 70 was significantly higher than that of the control group (P = 0.02). In patients with inactive disease it was higher compared to the controls but with a significance level close to 0.05 (P = 0.044). However, HSP 70 levels did not differ significantly between patients with active disease and patients with inactive disease (P = 0.93). Conclusion: The results of our study suggest that serum IL-6, IL-8, TNF-α, and CRP levels are increased in patients with active Behçet's disease. HSP 70 levels in both active and inactive disease groups were higher than in controls, though it was significant only in the active group. This suggests that HSP 70 has a role in the chronic nature of Behçet's disease, with HSP 70 expression possibly not falling to normal levels in the inactive phase of the disease.

Research paper thumbnail of Immunomodulatory role of leptin treatment in experimental sepsis caused by gram negative bacteria

Turkish Journal of Medical Sciences

To investigate the eff ect of leptin treatment on circulating infl ammatory cytokines and on tiss... more To investigate the eff ect of leptin treatment on circulating infl ammatory cytokines and on tissue damage in experimental rat model of gram-negative sepsis. Materials and methods: Adult male Wistar rats, 28 in total, were randomly divided into 4 groups (n = 7): sham, leptin, sepsis, and sepsis group treated with leptin (sepsis+leptin). Sepsis was induced by intraperitoneal (ip) injection of 2 × 10 10 CFU of Escherichia coli ATCC 25922. Leptin and sepsis+leptin groups received a single dose ip 0.1 mg/kg leptin, while sham group received 1 mL of ip saline. Rats were sacrifi ced 24 h aft er the induction of sepsis. Blood samples, lung, and kidney tissues were collected for analysis. Results: Th e sepsis group had signifi cantly higher serum TNF-α, IL-6, and endothelin levels than the sham group (P = 0.05, P < 0.001, P = 0.003, respectively). Th e sepsis+leptin group had signifi cantly lower IL-6 and endothelin levels (P = 0.001 and P = 0.020, respectively), and higher lung and kidney tissue myeloperoxidase activities when compared with the sepsis group (P = 0.039, P = 0.033, respectively). Conclusion: According to our results, leptin has a profound infl uence on sepsis, and to some extent it restricts the infl ammatory events in sepsis.

Research paper thumbnail of Subclinical hypoxia of infants with intrauterine growth retardation determined by increased serum S100B protein levels

Turkish Journal of Medical Sciences

To test the hypothesis that serum S100B levels could be useful in detecting neurological damage i... more To test the hypothesis that serum S100B levels could be useful in detecting neurological damage in infants with intrauterine growth retardation (IUGR). Materials and methods: Th e study group consisted of infants with IUGR and the control group consisted of agematched healthy infants. S100B protein levels were measured aft er birth and compared between groups. Results: For this study, 43 infants with IUGR and 25 infants as a control group were recruited. Gender, gestational age, type of delivery, and maternal age of the groups were statistically insignifi cant, with the exception of the mean birth weights (2120 ± 450 g in the IUGR group and 3096 ± 570 g in the control group (P < 0.001), respectively). S100B protein levels of the IUGR infants (1.13 ± 0.54) were signifi cantly higher than those of the control group (0.45 ± 0.13) (P < 0.001). IUGR infants treated with antenatal steroids showed lower S100B levels than IUGR infants that did not receive antenatal steroid treatments (P < 0.05). Th e study group infants were divided into 2 groups, for growth retardation (GR) that was asymmetric (n = 15) and symmetric (n = 28). Th e asymmetric and symmetric GR infants' S100B levels were 1.14 ± 0.47 pg/mL and 1.21 ± 0.34 pg/mL, respectively, and no signifi cant diff erences were found between the 2 groups in terms of S100B levels (P = 0.32). Conclusion: Th e results of this study favor the opinion that there is an existing intrauterine hypoxia causing hypoxic brain tissue damage in IUGR infants, even when followed up with modern obstetrical screening protocols. Measurements of S100B may be useful in the prediction of outcome in these infants.

Research paper thumbnail of Effects of Erdosteine in Experimental Sepsis Model in Rats

Hong Kong Journal of Emergency Medicine, 2011

Objective Erdosteine is a mucolytic agent that is known to possess antioxidant effects. This stud... more Objective Erdosteine is a mucolytic agent that is known to possess antioxidant effects. This study investigated the effects of erdosteine on endothelin-1 (ET-1) levels and oxidative stress parameters superoxide dismutase (SOD) and malondialdehyde (MDA) in a rat sepsis model. Methods Four groups of Wistar albino rats (n=8 per group) were randomly allocated to the following groups: sham (group 1), sepsis (group 2), erdosteine control (group 3) and a sepsis group pretreated with erdosteine (group 4). Sepsis was induced using E. Coli ATCC 25922 inoculation. Serum ET-1, liver tissue SOD and MDA levels were determined in all groups. Results ET-1 levels were significantly higher in group 2 compared to groups 1, 3 and 4 (p<0.001, p=0.002 and p<0.001, respectively). Similarly, MDA levels in groups 1, 3 and 4 were significantly lower relative to group 2 (p<0.001, p=0.022 and p=0.010, respectively). Additionally, SOD activities in these same three groups were found to be significantly...

Research paper thumbnail of Erratum for: Association of coronary artery disease severity and disulphide/native thiol ratio

Electronic Journal of General Medicine, 2018

Research paper thumbnail of Responses of Anterior Pituitary Hormones to Fever During Community-Acquired Infections

Electronic Journal of General Medicine, 2008

INTRODUCTION Fever is a part of the acute phase response (APR) to infection and systemic inflamma... more INTRODUCTION Fever is a part of the acute phase response (APR) to infection and systemic inflammation. It is a part of a complex physiological response of the host against microorganisms or foreign non-microbial agents invading the body (1,2). The fever is induced by infammatory mediators released by immune cells activated by contacts with foreign molecules (1,2). Recent studies have demonstrated that there are bidirectional circuits between the central nervous systems.(CNS) and the immune system 3,4. The hypothalamicpituitary-adrenal (HPA) axis plays an important role between the immune

Research paper thumbnail of The Effect of Dexmedetomidine and Propofol on Oxidative Stress Parameters during Lower Extremity Surgery: A Prospective Randomized Trial

International Journal of Anesthetics and Anesthesiology, 2015

Background: This study aims to compare the impact of sedation, continuous dexmedetomidine and pro... more Background: This study aims to compare the impact of sedation, continuous dexmedetomidine and propofol infusion, to oxidative stress that occurred as a result of tourniquet-induced ischemia reperfusion (IR) during lower extremity surgery. Material & Methods: All patients were administered combined spinoepidural anesthesia; Group D received infusion of 1µg kgdexmedetomidine for 10 minutes and 0.5µg kghinfusion, Group P was administered 0.2 mg kgpropofol following bolus 2 mg kgmin-. At baseline, 20 minutes and two hours after the tourniquet was released, plasma total antioxidant status (TAS), total oxidant status (TOS), paraoxonase (PON), stimulated paraoxonase (SPON), arylesterase, ceruloplasmin, myeloperoxidase (MPO), ischemia modified albumin (IMA) and advanced oxidation protein products (AOPP) levels were analyzed. Results: In Group D, ceruloplasmin values taken post tourniquet release were lower compared to the baseline values (p = 0.02 and 0.008, respectively). As for Group P, a decrease in the TAS and TOS values was recorded two hours after the tourniquets were released (in both p = 0.008). No differences were found in other markers of oxidative stress during intra-group comparisons (p > 0.05). Conclusion: The study concluded that administration of propofol and dexmedetomidine sedation during lower extremity surgery had similar effects on oxidative stress caused by tourniquet-induced IR.

Research paper thumbnail of A novel marker in acute central serous chorioretinopathy: thiol/disulfide homeostasis

International Ophthalmology, 2017

Purpose The aim of this study was to compare dynamic thiol/disulfide homeostatic status in acute ... more Purpose The aim of this study was to compare dynamic thiol/disulfide homeostatic status in acute central serous chorioretinopathy (CSCR) patients by using a novel and automated assay determining dynamic thiol/disulfide homeostasis.MethodsFifty-one patients with acute CSCR (study group) and 65 healthy individuals (control group) were enrolled in this study. Diagnosis of acute CSCR was made clinically and using spectral-domain RTVue OCT (optical coherence tomography) (Optovue, Fremont, CA). Fluorescein angiography confirmed the diagnosis of acute CSCR in all subjects. Total thiol, native thiol, disulfide amount, and native thiol/disulfide ratio (TDR) were calculated in the blood samples.ResultsMean total thiol, native thiol, and native TDR values were lower in patients with acute CSCR (364.2 ± 14.1, 326.4 ± 13.2, 17.14 ± 1.9, respectively) than in healthy eyes (441.2 ± 16.3, 398.5 ± 16.4, 22.70 ± 2.15, respectively; mean total thiol, p = 0.017; native thiol, p = 0.011; native TDR, p = 0.031).ConclusionsTotal thiol, native thiol, and native TDR were significantly lower statistically in patients with acute CSCR when compared with healthy controls.

Research paper thumbnail of Thiol/Disulfide Homeostasis in Patients with Central Serous Chorioretinopathy

Current Eye Research, 2016

To evaluate dynamic thiol/disulfide homeostasis in patients with chronic central serous chorioret... more To evaluate dynamic thiol/disulfide homeostasis in patients with chronic central serous chorioretinopathy (cCSC). This prospective study included 34 cCSC cases and 37 healthy individuals who were age- and sex-matched. A new colorimetric method for measuring thiol/disulfide homeostasis was used. Native thiol, total thiol/disulfide levels, disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios were measured. The age and gender distributions were similar in both main groups. The mean duration of disease was 47.29 ± 24 months. Native and total thiol levels were significantly lower among the cCSC group relative to the control group (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). There was not a statistically significant difference between the cCSC and the control group in terms of disulfide levels (p = 0.492). While disulfide/native thiol and disulfide/total thiol ratios were elevated, the native thiol/total thiol ratio was decreased in the cCSC group (p = 0.017, 0.021, 0.036, respectively). Ratios obtained using plasma native thiol, total thiol, and disulfide levels differed significantly between the both groups. Disulfide/thiol ratio was significantly greater in cCSC patients relative to healthy control subjects. Our results suggest that the oxidative process is involved in the pathogenesis of the cCSC.

Research paper thumbnail of Assessment of fetal antioxidant and oxidant status during different anesthesia techniques for elective cesarean sections

Journal of Research in Medical Sciences, 2015

and more popular because of the development of anesthesia techniques and materials besides patien... more and more popular because of the development of anesthesia techniques and materials besides patients can remain conscious. In the case of cesarean section surgery, for the determination of the method of anesthesia, not only patient and doctors preferences but also fetal status is important. Although general anesthesia is known to have negative effects on the baby's Apgar scores, [4] it is unavoidable for some patients who refused regional anesthesia due to fear of the needless, emergency conditions, and medical contraindication such as coagulation disorders, aortic stenosis, and infection. [5] Although oxidative and anti-oxidative stress marker levels were shown to be higher in cesarean section Background: We aimed to investigate the effects of general, spinal and epidural anesthesia on fetal total antioxidant status (TAS) and total oxidant status (TOS), and oxidative stress index (OSI) during elective cesarean section in this study. Materials and Methods: Forty-seven parturients scheduled for elective cesarean section were randomly allocated into three groups: Group spinal (n = 15), group epidural (n = 17), and group general (n = 15), This prospective randomized study was performed in Faculty of Medicine, Turgut Ozal University, Turkey. After the baby was delivered; TAS, TOS levels, and arterial blood gases parameters were analyzed in an umbilical arterial blood sample. OSI values are calculated by a ratio of TOS to the TAS. Results: The levels of TAS and TOS in umbilical arterial blood sample were not statistically different among three. However, OSI values were significantly different among the three groups (P = 0.042). Median OSI values is 24 (interquartile range [IQR], 2-37) in group spinal, 19 (IQR, 4-44) in group epidural, and 8 (IQR, 4-36) in group general. There was no significant difference in OSI values in the comparison of group spinal with group general and group epidural, but it was significantly lower in group general when compared with group epidural with Bonferroni correction (P = 0.017). Umbilical cord arterial blood gas values (pH, PaCO 2 , PaO 2 , SaO 2 , HCO 3 , and CtO 2), glucose, lactate, and hemoglobin levels were similar in three groups. Conclusion: General anesthesia may be more favorable than epidural in those undergoing cesarean section when fetal oxidative status gains importance.

Research paper thumbnail of Antioxidant effects of curcumin in spinal cord injury in rats

Turkish Journal of Trauma and Emergency Surgery, 2011

Bu deneysel çalışma, sıçanlarda spinal kord yaralanmasında curcuminin antioksidan etki yoluyla fa... more Bu deneysel çalışma, sıçanlarda spinal kord yaralanmasında curcuminin antioksidan etki yoluyla faydasını araştırmak için yapıldı. GEREÇ VE YÖNTEM Yirmi dört adet Wistar albino sıçan 3 gruba randomize edildi. Spinal kord yaralanması ağırlık düşürme modeliyle gerçekleştirildi. Grup 1'e laminektomi ardından spinal kord yaralanması uygulandı ve herhangi bir tedavi verilmedi. Grup 2'ye laminektomi ardından spinal kord yaralanması uygulandı ve curcumin verildi (200 mg/kg/gün ağızdan). Grup 3'e laminektomi ardından spinal kord yaralanması uygulandı ve metilprednizolon verildi (30 mg/kg periton içine), 24 saat sonra tüm sıçanlardan kan örnekleri alındı, sonra serum süperoksit dismutaz (SOD) ve malondialdehit (MDA) düzeyleri belirlendi ve elde edilen sonuçlar karşılaştırıldı. BULGULAR Curcumin grubunda SOD düzeyi kontrol ve metilprednizolon grubundan daha yüksekti (p<0,001 ve p<0,012). Curcumin grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,042). Benzer şekilde metilprednizolon grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,001). SONUÇ Bu çalışmanın sonuçları curcuminin etkin biçimde oksidatif hasara karşı spinal kord dokularını koruduğunu gösterir.

Research paper thumbnail of Value of Caffeic Acid Phenethyl Ester Pretreatment in Experimental Sepsis Model in Rats

Mediators of Inflammation, 2015

Background and Aim. The aim of this study was to determine the actions of caffeic acid phenethyl ... more Background and Aim. The aim of this study was to determine the actions of caffeic acid phenethyl ester (CAPE) on the changes of endothelin-1 (ET-1) level, tumor necrosis factor- (TNF-) alpha, and oxidative stress parameters such as superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in experimental sepsis model in rats.Materials and Methods. Twenty-four rats were randomly divided into three experimental groups: sham (group 1), sepsis (group 2), and sepsis + CAPE (group 3),n= 8 each. CAPE was administered (10 µmol/kg) intraperitoneally to group 3 before sepsis induction. Serum ET-1, serum TNF-alpha, tissue SOD activity, and tissue MDA levels were measured in all groups.Results. Pretreatment with CAPE decreased ET-1, TNF-alpha, and MDA levels in sepsis induced rats. Additionally SOD activities were higher in rats pretreated with CAPE after sepsis induction.Conclusion. Our results demonstrate that CAPE may have a beneficial effect on ET and TNF-alpha levels and oxida...

Research paper thumbnail of Lipid peroxidation and scavenging enzyme levels in the liver of streptozotocin-induced diabetic rats

Indian journal of biochemistry & biophysics, 2003

In this study, alterations in the liver antioxidant enzymes status and lipid peroxidation in shor... more In this study, alterations in the liver antioxidant enzymes status and lipid peroxidation in short-term (8-weeks) and long-term (24-weeks) diabetic rats were examined. Glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) levels were significantly increased, but superoxide dismutase (SOD) activity was significantly reduced in 8-weeks diabetic rats, compared to control. Catalase (CAT) activity, however, was found unchanged. In 24-weeks diabetic rats, while GSH-Px activity was unchanged, but SOD and CAT activities and MDA levels were significantly increased, compared to control. These results suggest that diabetes-induced alterations in tissue antioxidant system may reflect a generalized increase in tissue oxidative stress. It can be concluded that lipid peroxidation and antioxidant enzyme levels are elevated in diabetic condition. Hence, diabetes mellitus, if left untreated, may increase degenerative processes due to accumulation of oxidative free radicals.

Research paper thumbnail of Effects of Different Fluids on Hymodynamic Parameters and Liver Arginase Activities in Hemorrhagic Shock

AIM: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to res... more AIM: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to research the effect of different fluid resuscitation on the liver arginase activity. MATERIALS AND METHODS: Fifty rats were divided into five groups. In group 1; massive splenic injury was untreated, in group 2 massive injury was treated with 70 ml/kg/hour Ringer Lactate solution, in groups 3,4,5 massive injury was treated with 7.5 ml/kg/hour Hydroxyethyl Starch, Hypertonic Saline and Dextran-40, respectively between 15 and 30 minutes of injury. In all groups, splenectomy was performed at the 30th minute. Hemodynamic monitoring and anesthesia were continued up to 90 minutes. Duration of follow-up was 48 hrs. After 48 hours of survival, liver tissue samples were removed and were kept in -70◦C for the analysis of arginase activities. RESULTS: Different fluid regimens were found to have no effects on hemodynamic parameters, arteriel blood gases and survival at the early phase of uncontrolled hemorrhagic shock. RL increased liver arginase activity compared with control group (p<0.02) and other groups. (p<0.05). Unlike Ringer Lactate, other groups had no statistical differences at arginase activities comparing to control group. CONCLUSIONS: Finally, depending on long term results, Ringer Lactate is more useful fluid choice in hemorrhagic shock.

Research paper thumbnail of Decreased Plasma Vitamin D Levels in Patients with Undifferentiated Spondyloarthritis and Ankylosing Spondylitis

Internal Medicine, 2013

Objective The aim of the present study was to evaluate the plasma vitamin D (vit D) levels and th... more Objective The aim of the present study was to evaluate the plasma vitamin D (vit D) levels and their association with the disease activity in patients with ankylosing spondylitis (AS) and undifferentiated spondyloarthritis (uSpA) compared with healthy populations. Methods This study included 161 spondyloarthritis patients (113 uSpA patients and 48 AS patients) attending our rheumatology outpatient clinic, along with 92 controls. Results The plasma vit D levels were 18 μg/L (8-38) in the AS group, 20 μg/L (4-92.3) in the uSpA group and 24.3 μg/L (7.2-76.8) in the control group. The plasma vit D levels of the AS patients were significantly lower than those of the patients in the control group (p=0.004). The men in the AS group had significanly lower vit D levels than those in the control group (p=0.005). On the other hand, the women in the uSpA group had significanly lower vit D levels than those in the control group (p=0.011). The vit D levels were inversely related to both erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in the AS patients (p=0.002, R=-0.428; p<0.001, R=-0.592, respectively). This correlation was not demonstrated in the uSpA patients. The vit D levels were not found to correlate with the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) levels in either the AS or uSpA patients. Conclusion 25-hydroxy-vit D deficiency is frequently observed in patients with SpAs. In this study, vit D deficiency was much more prominent in the male AS patients. On the other hand, among women, the uSpA patients exhibited much more prominent vit D deficiency than the control group subjects. The acute phase response may inversely affect the vit D levels in AS patients.

Research paper thumbnail of Association of polycystic ovary syndrome and a non-dipping blood pressure pattern in young women

Clinics, 2010

OBJECTIVE: The association between polycystic ovarian syndrome and increased cardiovascular disea... more OBJECTIVE: The association between polycystic ovarian syndrome and increased cardiovascular disease risk is still a controversial issue. In light of data documenting some common pathways or common end-points, the present study was undertaken to determine whether there is a relationship between sleep blood pressure pattern disturbances and polycystic ovarian syndrome in young women. METHOD: The daytime and nighttime ambulatory blood pressures (BPs) were determined for each subject, according to the actual waking and sleeping times recorded in their individual diaries, in this cross-sectional study. RESULTS: The study group comprised 168 women (mean age: 25.7±5.5) diagnosed with polycystic ovarian syndrome, while the control group included 52 age-and BMI-matched healthy subjects (mean age: 26.1±5.4). When nocturnal BP declines very little or not at all, with the BP falling less than 10% during sleep compared with waking values, this pattern is classified as a non-dipping BP pattern. However, the non-dipping pattern of BP changes was significantly more common in polycystic ovarian syndrome patients compared to the control group (p<0.01). The prevalence of a non-dipping BP pattern was 43.4% (73 patients) in polycystic ovarian syndrome patients and 3.9% (2 patients) in the control group. CONCLUSION: Our cross-sectional study revealed that a non-dipping BP pattern is highly prevalent in polycystic ovarian syndrome patients, even if they are young and non-obese.

Research paper thumbnail of Evaluation of Asymmetric Dimethylarginine (ADMA) Levels in Children with Growth Hormone Deficiency

Journal of Clinical Research in Pediatric Endocrinology, 2014

Ori gi nal Ar tic le ABS TRACT Ob jec ti ve: To investigate serum asymmetric dimethylarginine (AD... more Ori gi nal Ar tic le ABS TRACT Ob jec ti ve: To investigate serum asymmetric dimethylarginine (ADMA) levels in children with isolated growth hormone deficiency (GHD) and to determine the effect of GH replacement therapy on these levels. Methods: 31 patients diagnosed with isolated GHD and 29 age-and sexmatched healthy children were enrolled in the study. Height, weight and waist circumference were measured in all subjects. Fasting serum insulin-like growth factor-1 (IGF-1), IGF binding protein-3, glucose, insulin and lipid levels were evaluated. Serum ADMA levels were assessed using the enzyme-linked immunosorbent assay technique. The same evaluations were repeated on the 3 rd and 6 th months of treatment in 28 of the GHD cases. Results: There were no significant differences in ADMA levels between the patient and control groups [0.513±0.130 (0.291-0.820) μmol/L vs. 0.573±0.199 (0.241-1.049) μmol/L]. There was a positive correlation between serum ADMA and HbA1c levels in the control group. In the GHD cases, ADMA levels negatively correlated with high-density lipoprotein levels and positively correlated with low-density lipoprotein levels. There was also a significant increase in ADMA levels in patients receiving GH therapy compared to pre-treatment levels [serum ADMA level, 1.075±0.133 (0.796-1.303) μmol/L at the 3 rd month and 0.923±0.121 (0.695-1.159) μmol/L at the 6 th month of treatment]. There was a negative correlation between ADMA levels and homeostasis model assessment of insulin resistance values at the 6 th month evaluation. There were no relationships between ADMA levels and age, sex, or pubertal state either before or during the treatment. Conclusion: Serum ADMA levels were found to be similar in patients with GHD and in healthy children. However, serum ADMA levels showed a significant increase in GHD patients following GH replacement therapy.

Research paper thumbnail of The Effect of Purified Micronized Flavonoid Fraction on the Healing of Anastomoses in the Colon in Rats

Surgery Today, 2006

collagenolysis, deposition of new collagen, and maturation. 1 There is a complex interaction of m... more collagenolysis, deposition of new collagen, and maturation. 1 There is a complex interaction of many factors and collagen turnover. 2 Anastomotic leakage is a major complication of surgery of the digestive tract. 3-5 Morbidity and mortality rates because of clinically evident leakage are still high, despite advances in operative techniques and suture materials. 4 The reported incidences of leakage of colorectal anastomoses vary greatly. Using contrast studies, leakage was detected in up to 50% of patients who underwent an anterior resection. 6,7 Clinically, apparent anastomotic leakage ranges from 3.4% to 8%. 8 Anastomotic leakage increases the hospital length of stay twofold and perioperative morbidity threefold. 9 Flavonoids are a chemically active heterogeneous group of plant extracts naturally occurring as food chemicals with considerable structural, biochemical, and pharmacological diversity. 10,11 The mechanisms of flavonoid action are multifactorial. Flavonoids have distinct effects on the function of immune and inflammatory cells both in vivo and in vitro: the release of inflammatory mediators and cytotoxic enzymes, the formation of oxygen radical species, and the homophilic and/or heterophilic adhesiveness of inflammatory cells. Flavonoids also have a beneficial action on collagen sythesis. 10 With the aforementioned effects of flavonoids, the objective of this study was to investigate the effects of clinically used, micronized, purified, flavonoid fractions (Daflon, 500 mg; Servier, Turkey) on the healing of anastomoses in the colon of rats. Methods The Ethics Committee of Fatih University Medical School approved this experimental protocol. The study was performed in accordance with the internationally accepted principles for laboratory animals.

Research paper thumbnail of Is IL-33 useful to detect early stage of renal failure?

Renal Failure, 2013

IL-33 is a proinflammatory cytokine that is a member of IL-1 family. Previously the effect of IL-... more IL-33 is a proinflammatory cytokine that is a member of IL-1 family. Previously the effect of IL-33 on kidney injury is showed in animal models. In this study, we searched if we can use IL-33 to show the early stage of kidney injury in diabetic patients. Three groups are identified: 26 patients in Group 1: Healthy group, that do not have any chronic diseases and not taking any medication; 42 patients in Group 2: DM (diabetes mellitus) group without any known kidney disease and with normal kidney functions; 32 patients in Group 3: DM + MA (microalbuminuria) group that are assumed to have nephropathy. IL-33 level of DM patient group is greater than healthy group; also IL-33 level of DM + MA patient group is greater than healthy group; but there is not any difference between DM and DM + MA group. The increase in IL-33 levels in diabetic nephropathy is not associated with kidney injury but the increase could be resulting because of diabetes. So IL-33 cannot be used in early recognition of diabetic nephropathy.

Research paper thumbnail of Nitric Oxide Affects Serum Ferritin Levels in Children with Iron Deficiency

Pediatric Hematology-Oncology, 2007

In iron deficiency, serum levels of ferritin decrease. The lack of iron has been thought to be th... more In iron deficiency, serum levels of ferritin decrease. The lack of iron has been thought to be the main factor in this decrease, but another potential factor is nitric oxide, which has been shown to affect ferritin metabolism in vitro. The aim of this study was therefore to evaluate in children with iron deficiency the relation of serum ferritin, nitric oxide degradation products (nitrate and nitrite), and endothelin-1, a protein closely related to nitric oxide function. A total of 80 children were included in the study (39 with iron deficiency, 41 controls). Serum levels of ferritin, nitrate, nitrite, and endothelin-1 were measured in all participants. In children with iron deficiency, nitrate and nitrite levels were significantly higher (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .009 and .01, respectively). Also, serum ferritin was negatively correlated with serum levels of nitrate and nitrite (p = .034, r = -.254 for nitrate and p = .01, r = -.593 for nitrite). No statistical relationship was found between serum ferritin and endothelin-1.