Md Sarowar Alam | Bangladesh Agricultural Research Institute (BARI) (original) (raw)
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Papers by Md Sarowar Alam
Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using ... more Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using molecular markers during June 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. FL-378 was identified as donor or male parent for saltol QTL and Binadhan-7 as recurrent or recipient parent which had high yield with short life cycle. Crossing was done between them and 10 F 1 seeds were produced. PCR bands from all the 10 F 1 plants were scored as "H" represented heterozygous alleles for donor and recipient parent. Backcrossing was done to produce 105 BC 1 F 1 seedlings. Foreground selection was performed with 72 BC 1 F 1 plants with a tightly linked salt tolerance marker RM21. Out of 72 plants, 33 plants were selected for RM21 showing 'H' scores. The selected segregants were subjected to further recombinant and background selections at BC 1 F 1 generation. These selected genotypess could be used for further foreground, recombinant and background selections with appropriate markers upto BC 3 generation for the development of salt tolerant rice genotypes.
Pummelo is one of the basic citrus species of horticultural importance for human health and nutri... more Pummelo is one of the basic citrus species of horticultural importance for human health and nutrition security. It has wide genetic diversity due to its breeding behavior during the domestication process. The present research was performed to study the fruits traits and characterization of the studied 21 pummelo genotypes with morphological, fruit quality parameters, and SSR markers. Principal component analysis (PCA) was done to get PC individual plot and clustering the genotypes in five main clusters based on 17 morphological traits. The highest number (08) of genotypes was grouped in cluster V and the lowest (01) in cluster III. Genotypes resided with the highest genetic distance in the individual biplots are promising for suitable parents. Again, molecular characterization or cluster analysis was performed with 5 SSR markers. The PIC values ranged from a low of 0.66 (CAC23) to a high of 0.8787 (TAA41) and averaged 0.78. TAA41 presented the second largest (47 bp) difference among allele size range and the largest (11) number of alleles with the highest gene diversity value (0.88). The average genetic distance among the 21 pummelo genotypes were 0.8362. The NJ cluster analysis led to the grouping of the pummelo genotypes in three major clusters (I, II, and III). The genotypes resided in the similar cluster should be avoided for pummelo improvement. The findings of this research would be useful to select desirable parents for future pummelo breeding program.
Marker-assisted Backcrossing for Identification of Salt Tolerant Rice Lines, 2012
SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection... more SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection and studying genetic diversity in germplasm. SSR markers are playing important role to identify gene for salt tolerance that can be helpful for plant breeders to develop new cultivars. The experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to identify salt tolerant rice line of BC1F1 progenies of Binadhan-5 x FL-478 using SSR markers. Salt tolerant genotype, FL-478 was crossed with high yielding variety, Binadhan-5. Randomly selected 40 BC1F1 progenies along with their two parents (Binadhan-5, FL-478 and F1) were genotyped with microsatellite or SSR markers for identification of salt tolerant rice lines. Parental polymorphism survey was assayed by 10 SSR markers and three polymorphic SSR markers viz., RM 336, RM 510, and RM 585 were selected to evaluate BC1F1 rice lines for salt tolerance. In respect of Primer RM 336, 11 lines were found as salt tolerant and 25 lines were heterozygous and 3 lines were susceptible. Primer RM 510 identified two tolerant, 14 heterozygous and 22 susceptible lines. And primer RM 585 identified 4 lines as tolerant and 35 lines as susceptible. Thus, these markers could be efficiently used in tagging salt tolerant genes, in marker-assisted selection and quantitative trait loci (QTL) mapping. The selected BC1F1 could be used for developing BC2F1 and BC2F2 and mapping genes for salinity tolerance.
The experiment was conducted during 2019-2021 at Regional Agricultural Research Station, Akbarpur... more The experiment was conducted during 2019-2021 at Regional Agricultural Research Station, Akbarpur, Moulvibazar, Bangladesh with twenty-three local mango genotypes to assess the performance and genetic divergence for the identification of suitable parents for the futute breeding program.. Wide variations were observed regarding the number of fruits/plant, fruit weight, percent of edible portion, and TSS of different germplasms. The highest number of fruit per plant (610) was observed in MIAkb001, highest individual fruit weight /plant (759.7g) was obtained from MIAkb015. The higher fruit yield was recorded from MIAkb001 (162.07kg). The percent of the edible portion was higher in MIAkb014 (65.71%) whereas maximum TSS (18%) was obtained from MIAkb007 andMIAkb015 respectively. The genetic divergence was assessed in 23 mango genotypes through multivariate analysis. The genotypes under this study were grouped into five clusters and the diversity was influenced by the morphological characters. The highest number (8) of genotypes were in cluster-I and the lowest (1) number of the genotype was in cluster-III. From the PCA we found, the number of fruits per plant (FNPP), fruits yield per plant (FYPP), and individual fruit weight were positive across the two axes, and these were the main traits for genetic divergence.
Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the ... more Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding.
Plants, 2021
Salinity is one of the most significant environmental stresses for sustainable crop production in... more Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most su...
Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using ... more Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using molecular markers during June 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. FL-378 was identified as donor or male parent for saltol QTL and Binadhan-7 as recurrent or recipient parent which had high yield with short life cycle. Crossing was done between them and 10 F 1 seeds were produced. PCR bands from all the 10 F 1 plants were scored as "H" represented heterozygous alleles for donor and recipient parent. Backcrossing was done to produce 105 BC 1 F 1 seedlings. Foreground selection was performed with 72 BC 1 F 1 plants with a tightly linked salt tolerance marker RM21. Out of 72 plants, 33 plants were selected for RM21 showing 'H' scores. The selected segregants were subjected to further recombinant and background selections at BC 1 F 1 generation. These selected genotypess could be used for further foreground, recombinant and background selections with appropriate markers upto BC 3 generation for the development of salt tolerant rice genotypes.
Pummelo is one of the basic citrus species of horticultural importance for human health and nutri... more Pummelo is one of the basic citrus species of horticultural importance for human health and nutrition security. It has wide genetic diversity due to its breeding behavior during the domestication process. The present research was performed to study the fruits traits and characterization of the studied 21 pummelo genotypes with morphological, fruit quality parameters, and SSR markers. Principal component analysis (PCA) was done to get PC individual plot and clustering the genotypes in five main clusters based on 17 morphological traits. The highest number (08) of genotypes was grouped in cluster V and the lowest (01) in cluster III. Genotypes resided with the highest genetic distance in the individual biplots are promising for suitable parents. Again, molecular characterization or cluster analysis was performed with 5 SSR markers. The PIC values ranged from a low of 0.66 (CAC23) to a high of 0.8787 (TAA41) and averaged 0.78. TAA41 presented the second largest (47 bp) difference among allele size range and the largest (11) number of alleles with the highest gene diversity value (0.88). The average genetic distance among the 21 pummelo genotypes were 0.8362. The NJ cluster analysis led to the grouping of the pummelo genotypes in three major clusters (I, II, and III). The genotypes resided in the similar cluster should be avoided for pummelo improvement. The findings of this research would be useful to select desirable parents for future pummelo breeding program.
Marker-assisted Backcrossing for Identification of Salt Tolerant Rice Lines, 2012
SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection... more SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection and studying genetic diversity in germplasm. SSR markers are playing important role to identify gene for salt tolerance that can be helpful for plant breeders to develop new cultivars. The experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to identify salt tolerant rice line of BC1F1 progenies of Binadhan-5 x FL-478 using SSR markers. Salt tolerant genotype, FL-478 was crossed with high yielding variety, Binadhan-5. Randomly selected 40 BC1F1 progenies along with their two parents (Binadhan-5, FL-478 and F1) were genotyped with microsatellite or SSR markers for identification of salt tolerant rice lines. Parental polymorphism survey was assayed by 10 SSR markers and three polymorphic SSR markers viz., RM 336, RM 510, and RM 585 were selected to evaluate BC1F1 rice lines for salt tolerance. In respect of Primer RM 336, 11 lines were found as salt tolerant and 25 lines were heterozygous and 3 lines were susceptible. Primer RM 510 identified two tolerant, 14 heterozygous and 22 susceptible lines. And primer RM 585 identified 4 lines as tolerant and 35 lines as susceptible. Thus, these markers could be efficiently used in tagging salt tolerant genes, in marker-assisted selection and quantitative trait loci (QTL) mapping. The selected BC1F1 could be used for developing BC2F1 and BC2F2 and mapping genes for salinity tolerance.
The experiment was conducted during 2019-2021 at Regional Agricultural Research Station, Akbarpur... more The experiment was conducted during 2019-2021 at Regional Agricultural Research Station, Akbarpur, Moulvibazar, Bangladesh with twenty-three local mango genotypes to assess the performance and genetic divergence for the identification of suitable parents for the futute breeding program.. Wide variations were observed regarding the number of fruits/plant, fruit weight, percent of edible portion, and TSS of different germplasms. The highest number of fruit per plant (610) was observed in MIAkb001, highest individual fruit weight /plant (759.7g) was obtained from MIAkb015. The higher fruit yield was recorded from MIAkb001 (162.07kg). The percent of the edible portion was higher in MIAkb014 (65.71%) whereas maximum TSS (18%) was obtained from MIAkb007 andMIAkb015 respectively. The genetic divergence was assessed in 23 mango genotypes through multivariate analysis. The genotypes under this study were grouped into five clusters and the diversity was influenced by the morphological characters. The highest number (8) of genotypes were in cluster-I and the lowest (1) number of the genotype was in cluster-III. From the PCA we found, the number of fruits per plant (FNPP), fruits yield per plant (FYPP), and individual fruit weight were positive across the two axes, and these were the main traits for genetic divergence.
Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the ... more Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding.
Plants, 2021
Salinity is one of the most significant environmental stresses for sustainable crop production in... more Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most su...