Ambuja Udas | Bhabha Atomic Research Centre (original) (raw)
Papers by Ambuja Udas
Efficiency of uptake of known pollutant heavy metals Hg, Cd, U and Zn by Mimosa pudica, a non-con... more Efficiency of uptake of known pollutant heavy metals Hg, Cd, U and Zn by Mimosa pudica, a non-consumable wild plant species, was evaluated. Various concentrations of heavy metals ranging from 1-10, 1-20, 5-20 and 50-200 mM were used and maximum uptake by the roots at 10, 20, 10 and 100 μM recorded for Hg, Cd, U, and Zn, respectively. The translocation of metals from root to stem (48%) and leaves (8%) was highest for Zn, as compared to others. U showed only 35% translocation to stem, where as Cd and Hg translocation to both stem and leaves, was negligible. The results indicated differential uptake for different metals by Mimosa pudica. The roots showed maximum uptake capacity for heavy metals, implying the possible utility of Mimosa pudica for rhizofiltration.
Atomic Spectroscopy, 2013
ABSTRACT
Ecological Engineering, 2014
ABSTRACT A field experiment was conducted in a combinatorial approach to evaluate the performance... more ABSTRACT A field experiment was conducted in a combinatorial approach to evaluate the performance of five aquatic plants, singly and in all possible combinations of two plants, for As removal from 4 L of 2500 μg L−1 As in 30 days. Under As stress, Hydrilla showed an increase in growth rate (19%) in comparison to control, while other plants demonstrated a decline. Among different combinations, Ceratophyllum + Lemna, Hydrilla + Ceratophyllum and Hydrilla + Wolffia showed higher growth in As (92%, 43%, and 33%, respectively) than under control conditions. Total As removal (μg) in 30 days was found to be the maximum for Ceratophyllum + Lemna (4365) followed by Hydrilla + Ceratophyllum (3326) and Hydrilla + Wolffia (1896). In all combinations of plants, Hydrilla always contributed more than 50%. The study advocates the use of Hydrilla/Ceratophyllum as one plant of choice to be used in combination. It also proposes to screen for different combinations of two of more plants for better application of phytoremediation technology in field.
Indian journal of clinical biochemistry : IJCB, 2014
A study of iron, zinc, copper and selenium concentration levels was carried out in three compartm... more A study of iron, zinc, copper and selenium concentration levels was carried out in three compartments namely, maternal serum (MS), colostrums and cord blood serum (CS) of healthy Indian mothers (n = 42) who delivered healthy normal neonates without any congenital anomalies at Bhabha Atomic Research Centre hospital, Mumbai. Fe, Zn, Cu in maternal serum, cord blood and colostrums were estimated by flame atomic absorption spectrometry while Se was determined by graphite furnace absorption spectrometry. It was seen that there was a significant difference in the level of trace elements in the three compartments. The average levels of Fe in the three compartments were 1,132 ± 519, 2,312 ± 789 and 1,183 ± 602 μg/L while Zn was 514 ± 149, 819 ± 224 and 7,148 ± 2,316 μg/L respectively. Mean Cu values were 1,614 ± 295, 301 ± 77 and 392 ± 174 μg/L respectively while Se values were 70 ± 15, 36 ± 10 and 23 ± 8 μg/L respectively. The results indicated a positive correlation of Fe and Zn concentra...
Journal of Hazardous Materials, 2007
Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, ... more Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, which can be quantitatively extracted into certain organic solvents. In the present work, 12-molybdoarsenic acid extracted in butan-1-ol is used for quantification of As (V). Total arsenic is estimated by converting arsenic (III) to arsenic (V) by digesting samples with concentrated nitric acid before extraction. Concentration of As (III) in the sample solutions could be calculated by the difference in total arsenic and arsenic (V). The characterization of arsenic was carried out by GFAAS using Pd as modifier. Optimization of the experimental conditions and instrumental parameters was investigated in detail. Recoveries of (90-110%) were obtained in the spiked samples. The detection limit was 0.2 microg l(-1). The proposed method was successfully applied for the determination of trace amount of arsenic (III) and arsenic (V) in process water samples.
ins-india.org
Page 1. Phosphatase mediated bioremediation of Cadmium Chitra Seetharam 1* , S. Suvarna 2 , AC Ud... more Page 1. Phosphatase mediated bioremediation of Cadmium Chitra Seetharam 1* , S. Suvarna 2 , AC Udas 2 , AS Rao 1 , M. Sudersenan 2 and SK Apte 1 ... Chitra Seetharam 1* , S. Suvarna 2 , AC Udas 2 , AS Rao 1 , M. Sudersenan 2 and SK Apte 1 ...
Indian Journal of Clinical Biochemistry
Anal. Methods, 2015
A simple method for the characterization and quantification of ultratrace quantities of Se(iv) in... more A simple method for the characterization and quantification of ultratrace quantities of Se(iv) in environmental water samples by preconcentration of selenium(iv) on PdNPs was optimized by employing the multivariate strategy.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 2013
Oral cancer is a major cause of cancer morbidity and mortality worldwide and is prevalent in most... more Oral cancer is a major cause of cancer morbidity and mortality worldwide and is prevalent in most areas where tobacco related practices are observed. Essential elements play a role in many biochemical reactions as a micro-source and there is growing evidence that their concentrations are altered on the onset and progress of malignant disease. In this study the levels of copper (Cu), zinc (Zn), selenium (Se) and molybdenum (Mo) in serum of patients with oral sub mucous fibrosis (OSMF) (n = 30) and oral squamous cell carcinoma (OSCC) (n = 30); were determined and the alterations of these critical parameters were analyzed in comparison with controls (n = 30) to identify predictors amongst these parameters for disease occurrence and progression. The serum Cu and Zn were established using Flame Atomic Absorption Spectrometry. Serum estimation of Se and Mo was done by graphite furnace atomic absorption spectrometry (GFAAS). Data analysis revealed a marked, progressive and significant incr...
Indian journal of clinical biochemistry : IJCB, 2014
A study of iron, zinc, copper and selenium concentration levels was carried out in three compartm... more A study of iron, zinc, copper and selenium concentration levels was carried out in three compartments namely, maternal serum (MS), colostrums and cord blood serum (CS) of healthy Indian mothers (n = 42) who delivered healthy normal neonates without any congenital anomalies at Bhabha Atomic Research Centre hospital, Mumbai. Fe, Zn, Cu in maternal serum, cord blood and colostrums were estimated by flame atomic absorption spectrometry while Se was determined by graphite furnace absorption spectrometry. It was seen that there was a significant difference in the level of trace elements in the three compartments. The average levels of Fe in the three compartments were 1,132 ± 519, 2,312 ± 789 and 1,183 ± 602 μg/L while Zn was 514 ± 149, 819 ± 224 and 7,148 ± 2,316 μg/L respectively. Mean Cu values were 1,614 ± 295, 301 ± 77 and 392 ± 174 μg/L respectively while Se values were 70 ± 15, 36 ± 10 and 23 ± 8 μg/L respectively. The results indicated a positive correlation of Fe and Zn concentra...
Talanta, 2009
a b s t r a c t Hydrazine (HZ) and sodium borohydride (BH) are commonly used reagents for the pro... more a b s t r a c t Hydrazine (HZ) and sodium borohydride (BH) are commonly used reagents for the production of palladium nanoparticles (PdNP) in aqueous solution and also for the reduction of arsenic from higher oxidation state to lower oxidation state. A methodology based on the quantitative adsorption of reduced arsenic species on PdNP generated in situ by BH and HZ is described to characterize As (V) and As (III) in environmental water samples. It was observed that PdNP obtained by BH gave quantitative recovery of As (V) and (III) and the PdNP obtained by HZ could account for As (III). The reduced palladium particles are collected and dissolved in minimum amount of nitric acid. The quantification of arsenic was carried out using GFAAS. Optimization of the experimental conditions and instrumental parameters were investigated in detail. The proposed procedure was validated by applying it for the determination of the content of total As in Certified Reference Material BND 301-02 (NPL, India). The detection limit of arsenic in environmental water samples was 0.029 g L −1 with an enrichment factor of 50. The relative standard deviation (R.S.D.) for 10 replicate measurements of 5 g mL −1 was 4.2%. The proposed method was successfully applied for the determination of sub ppm to ppm levels of arsenic (V), (III) in environmental water samples.
Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, ... more Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, which can be quantitatively extracted into certain organic solvents. In the present work, 12-molybdoarsenic acid extracted in butan-1-ol is used for quantification of As (V). Total arsenic is estimated by converting arsenic (III) to arsenic (V) by digesting samples with concentrated nitric acid before extraction. Concentration of As (III) in the sample solutions could be calculated by the difference in total arsenic and arsenic (V). The characterization of arsenic was carried out by GFAAS using Pd as modifier. Optimization of the experimental conditions and instrumental parameters was investigated in detail. Recoveries of (90-110%) were obtained in the spiked samples. The detection limit was 0.2 g l −1 . The proposed method was successfully applied for the determination of trace amount of arsenic (III) and arsenic (V) in process water samples.
Efficiency of uptake of known pollutant heavy metals Hg, Cd, U and Zn by Mimosa pudica, a non-con... more Efficiency of uptake of known pollutant heavy metals Hg, Cd, U and Zn by Mimosa pudica, a non-consumable wild plant species, was evaluated. Various concentrations of heavy metals ranging from 1-10, 1-20, 5-20 and 50-200 mM were used and maximum uptake by the roots at 10, 20, 10 and 100 μM recorded for Hg, Cd, U, and Zn, respectively. The translocation of metals from root to stem (48%) and leaves (8%) was highest for Zn, as compared to others. U showed only 35% translocation to stem, where as Cd and Hg translocation to both stem and leaves, was negligible. The results indicated differential uptake for different metals by Mimosa pudica. The roots showed maximum uptake capacity for heavy metals, implying the possible utility of Mimosa pudica for rhizofiltration.
Atomic Spectroscopy, 2013
ABSTRACT
Ecological Engineering, 2014
ABSTRACT A field experiment was conducted in a combinatorial approach to evaluate the performance... more ABSTRACT A field experiment was conducted in a combinatorial approach to evaluate the performance of five aquatic plants, singly and in all possible combinations of two plants, for As removal from 4 L of 2500 μg L−1 As in 30 days. Under As stress, Hydrilla showed an increase in growth rate (19%) in comparison to control, while other plants demonstrated a decline. Among different combinations, Ceratophyllum + Lemna, Hydrilla + Ceratophyllum and Hydrilla + Wolffia showed higher growth in As (92%, 43%, and 33%, respectively) than under control conditions. Total As removal (μg) in 30 days was found to be the maximum for Ceratophyllum + Lemna (4365) followed by Hydrilla + Ceratophyllum (3326) and Hydrilla + Wolffia (1896). In all combinations of plants, Hydrilla always contributed more than 50%. The study advocates the use of Hydrilla/Ceratophyllum as one plant of choice to be used in combination. It also proposes to screen for different combinations of two of more plants for better application of phytoremediation technology in field.
Indian journal of clinical biochemistry : IJCB, 2014
A study of iron, zinc, copper and selenium concentration levels was carried out in three compartm... more A study of iron, zinc, copper and selenium concentration levels was carried out in three compartments namely, maternal serum (MS), colostrums and cord blood serum (CS) of healthy Indian mothers (n = 42) who delivered healthy normal neonates without any congenital anomalies at Bhabha Atomic Research Centre hospital, Mumbai. Fe, Zn, Cu in maternal serum, cord blood and colostrums were estimated by flame atomic absorption spectrometry while Se was determined by graphite furnace absorption spectrometry. It was seen that there was a significant difference in the level of trace elements in the three compartments. The average levels of Fe in the three compartments were 1,132 ± 519, 2,312 ± 789 and 1,183 ± 602 μg/L while Zn was 514 ± 149, 819 ± 224 and 7,148 ± 2,316 μg/L respectively. Mean Cu values were 1,614 ± 295, 301 ± 77 and 392 ± 174 μg/L respectively while Se values were 70 ± 15, 36 ± 10 and 23 ± 8 μg/L respectively. The results indicated a positive correlation of Fe and Zn concentra...
Journal of Hazardous Materials, 2007
Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, ... more Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, which can be quantitatively extracted into certain organic solvents. In the present work, 12-molybdoarsenic acid extracted in butan-1-ol is used for quantification of As (V). Total arsenic is estimated by converting arsenic (III) to arsenic (V) by digesting samples with concentrated nitric acid before extraction. Concentration of As (III) in the sample solutions could be calculated by the difference in total arsenic and arsenic (V). The characterization of arsenic was carried out by GFAAS using Pd as modifier. Optimization of the experimental conditions and instrumental parameters was investigated in detail. Recoveries of (90-110%) were obtained in the spiked samples. The detection limit was 0.2 microg l(-1). The proposed method was successfully applied for the determination of trace amount of arsenic (III) and arsenic (V) in process water samples.
ins-india.org
Page 1. Phosphatase mediated bioremediation of Cadmium Chitra Seetharam 1* , S. Suvarna 2 , AC Ud... more Page 1. Phosphatase mediated bioremediation of Cadmium Chitra Seetharam 1* , S. Suvarna 2 , AC Udas 2 , AS Rao 1 , M. Sudersenan 2 and SK Apte 1 ... Chitra Seetharam 1* , S. Suvarna 2 , AC Udas 2 , AS Rao 1 , M. Sudersenan 2 and SK Apte 1 ...
Indian Journal of Clinical Biochemistry
Anal. Methods, 2015
A simple method for the characterization and quantification of ultratrace quantities of Se(iv) in... more A simple method for the characterization and quantification of ultratrace quantities of Se(iv) in environmental water samples by preconcentration of selenium(iv) on PdNPs was optimized by employing the multivariate strategy.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 2013
Oral cancer is a major cause of cancer morbidity and mortality worldwide and is prevalent in most... more Oral cancer is a major cause of cancer morbidity and mortality worldwide and is prevalent in most areas where tobacco related practices are observed. Essential elements play a role in many biochemical reactions as a micro-source and there is growing evidence that their concentrations are altered on the onset and progress of malignant disease. In this study the levels of copper (Cu), zinc (Zn), selenium (Se) and molybdenum (Mo) in serum of patients with oral sub mucous fibrosis (OSMF) (n = 30) and oral squamous cell carcinoma (OSCC) (n = 30); were determined and the alterations of these critical parameters were analyzed in comparison with controls (n = 30) to identify predictors amongst these parameters for disease occurrence and progression. The serum Cu and Zn were established using Flame Atomic Absorption Spectrometry. Serum estimation of Se and Mo was done by graphite furnace atomic absorption spectrometry (GFAAS). Data analysis revealed a marked, progressive and significant incr...
Indian journal of clinical biochemistry : IJCB, 2014
A study of iron, zinc, copper and selenium concentration levels was carried out in three compartm... more A study of iron, zinc, copper and selenium concentration levels was carried out in three compartments namely, maternal serum (MS), colostrums and cord blood serum (CS) of healthy Indian mothers (n = 42) who delivered healthy normal neonates without any congenital anomalies at Bhabha Atomic Research Centre hospital, Mumbai. Fe, Zn, Cu in maternal serum, cord blood and colostrums were estimated by flame atomic absorption spectrometry while Se was determined by graphite furnace absorption spectrometry. It was seen that there was a significant difference in the level of trace elements in the three compartments. The average levels of Fe in the three compartments were 1,132 ± 519, 2,312 ± 789 and 1,183 ± 602 μg/L while Zn was 514 ± 149, 819 ± 224 and 7,148 ± 2,316 μg/L respectively. Mean Cu values were 1,614 ± 295, 301 ± 77 and 392 ± 174 μg/L respectively while Se values were 70 ± 15, 36 ± 10 and 23 ± 8 μg/L respectively. The results indicated a positive correlation of Fe and Zn concentra...
Talanta, 2009
a b s t r a c t Hydrazine (HZ) and sodium borohydride (BH) are commonly used reagents for the pro... more a b s t r a c t Hydrazine (HZ) and sodium borohydride (BH) are commonly used reagents for the production of palladium nanoparticles (PdNP) in aqueous solution and also for the reduction of arsenic from higher oxidation state to lower oxidation state. A methodology based on the quantitative adsorption of reduced arsenic species on PdNP generated in situ by BH and HZ is described to characterize As (V) and As (III) in environmental water samples. It was observed that PdNP obtained by BH gave quantitative recovery of As (V) and (III) and the PdNP obtained by HZ could account for As (III). The reduced palladium particles are collected and dissolved in minimum amount of nitric acid. The quantification of arsenic was carried out using GFAAS. Optimization of the experimental conditions and instrumental parameters were investigated in detail. The proposed procedure was validated by applying it for the determination of the content of total As in Certified Reference Material BND 301-02 (NPL, India). The detection limit of arsenic in environmental water samples was 0.029 g L −1 with an enrichment factor of 50. The relative standard deviation (R.S.D.) for 10 replicate measurements of 5 g mL −1 was 4.2%. The proposed method was successfully applied for the determination of sub ppm to ppm levels of arsenic (V), (III) in environmental water samples.
Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, ... more Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, which can be quantitatively extracted into certain organic solvents. In the present work, 12-molybdoarsenic acid extracted in butan-1-ol is used for quantification of As (V). Total arsenic is estimated by converting arsenic (III) to arsenic (V) by digesting samples with concentrated nitric acid before extraction. Concentration of As (III) in the sample solutions could be calculated by the difference in total arsenic and arsenic (V). The characterization of arsenic was carried out by GFAAS using Pd as modifier. Optimization of the experimental conditions and instrumental parameters was investigated in detail. Recoveries of (90-110%) were obtained in the spiked samples. The detection limit was 0.2 g l −1 . The proposed method was successfully applied for the determination of trace amount of arsenic (III) and arsenic (V) in process water samples.