Teresa Altabella - Profile on Academia.edu (original) (raw)

Papers by Teresa Altabella

International Journal of Systematic and Evolutionary Microbiology

In the framework of the research project called fitomatics, we have isolated and characterized a ... more In the framework of the research project called fitomatics, we have isolated and characterized a bacterial plant-endophyte from the rhizomes of Iris germanica, hereafter referred to as strain FIT81T. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase- and oxidase-positive. The optimal growth temperature of strain FIT81T is 28 °C, although it can grow within a temperature range of 4–32 °C. The pH growth tolerance ranges between pH 5 and 10, and it tolerates 4% (w/v) NaCl. A 16S rRNA phylogenetic analysis positioned strain FIT81T within the genus Pseudomonas , and multilocus sequence analysis revealed that Pseudomonas gozinkensis IzPS32dT, Pseudomonas glycinae MS586T, Pseudomonas allokribbensis IzPS23T, 'Pseudomonas kribbensis' 46–2 and Pseudomonas koreensis PS9-14T are the top five most closely related species, which were selected for further genome-to-genome comparisons, as well as for physiological and chemotaxonomic characterization. The g...

Plant Physiology, Mar 1, 1990

The common bean, Phaseolus vulgaris, contains a glycoprotein that inhibits the activity of mammal... more The common bean, Phaseolus vulgaris, contains a glycoprotein that inhibits the activity of mammalian and insect a-amylases, but not of plant a-amylases. It is therefore classified as an antifeedant or seed defense protein. In P. vulgaris cv Greensleeves, a-amy- lase inhibitor (aAI) is present in embryonic axes and cotyledons, but not in other organs of the plant. The protein is synthesized during the same time period that phaseolin and phytohemagglutinin are made and also accumulates in the protein storage vacuoles (protein bodies). Purified aAl can be resolved by SDS- PAGE into five bands (Mr 15,000-19,000), four of which have covalently attached glycans. These bands represent glycoforms of two different polypeptides. All the glycoforms have complex glycans that are resistant to removal by endoglycosidase H, indicating transport of the protein through the Golgi apparatus. The two different polypeptides correspond to the N-terminal and C-terminal halves of a lectin-like protein encoded by an already identified gene or a gene closely related to it (LM Hoffman [1984]

Frontiers in Plant Science, May 8, 2018

Tomato Sterol Acyltransferases transcriptional response to several stresses suggests a dual role ... more Tomato Sterol Acyltransferases transcriptional response to several stresses suggests a dual role of SlPSAT1 and SlASAT1 in tomato plant and fruit development and the adaptive responses to stress. Overall, this study contributes to enlarge the current knowledge on plant sterol acyltransferases and set the basis for further studies aimed at understanding the role of SE metabolism in tomato plant growth and development.

Resistance to Botrytis cinerea is induced in an Arabidopsis mutant with reduced levels of sterol glycosides

Trabajo presentado a la XXIII Reunion Bianual de la Sociedad Espanola de Fisiologia Vegetal y al ... more Trabajo presentado a la XXIII Reunion Bianual de la Sociedad Espanola de Fisiologia Vegetal y al XVI Congreso Hispano-Luso de Fisiologia Vegetal, celebrados en Pamplona (Espana) del 26 al 28 de junio de 2019.

Polyamine content and regulation during pre-penetration and penetration resistance events in oat against crown rust (Puccinia coronata f. sp. avenae)

CRISPR/Cas9-mediated inactivation of Sterol Acyltransferases and its effect in tomato plant growth and development

Trabajo presentado al Internal Seminar of the Centre de Recerca Agrigenómica (CRAG), celebrado el... more Trabajo presentado al Internal Seminar of the Centre de Recerca Agrigenómica (CRAG), celebrado el 8 de marzo de 2019

Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development

Environmental and Experimental Botany

Glycosylated sterols play an important role in tomato plant and fruit development

Trabajo presentado al XV Meeting of Plant Molecular Biology, celebrado online del 26 al 27 de nov... more Trabajo presentado al XV Meeting of Plant Molecular Biology, celebrado online del 26 al 27 de noviembre de 2020.Peer reviewe

Omics and Plant Abiotic Stress Tolerance

Trends in Plant Science, 1997

Polyamines are low molecular mass polycations found in all living organisms. In plants, they have... more Polyamines are low molecular mass polycations found in all living organisms. In plants, they have been implicated in a wide range of biological processes, including growth, development and stress responses. Recently, several key genes of the polyamine biosynthesis pathways have been cloned and shown to be developmentally regulated. Experiments in which the synthesis of polyamines has been perturbed have clearly shown that these compounds are essential for normal growth and development. These findings have been further supported by recent genetic analyses of polyamine synthesis mutants of Arabidopsis. The research has revealed a much more detailed level of understanding than hitherto, opening up new avenues of investigation for the future. p olyamines form a class of aliphatic amines that are ubiquitous in living organisms, and have been implicated in a wide range of biological processes, including plant growth and development. The major forms are putrescine (butan-l,4-diamine), spermidine [N-(3-aminopropyl) butane-l,4-diamine] and spermine [NN'-bis-(3aminopropyl) butane-l,4-diamine]. At cellular pH values, these compounds behave as cations, and can interact with

Proteomics: emerging tools to characterize plant metabolons

International Journal of Systematic and Evolutionary Microbiology

Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-... more Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28T, was isolated. FIT28T shows vigorous growth on nutrient rich media within the temperature range of 4–35 °C, with optimal growth at 28 °C, a wide pH tolerance from pH 5 to 11, and salt tolerance up to 6 % (w/v) NaCl. Colonies are white-yellow and quickly become mucoid. The results of analysis of the 16S rRNA gene sequence placed the strain within the genus Pseudomonas , and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB, gyrB and rpoD concatenated sequences revealed that the closest relatives of FIT28T are Pseudomonas zeae OE48.2T, ' Pseudomonas crudilactis ' UCMA 17988, Pseudomonas tensinigenes ZA5.3T, Pseudomonas helmanticensis OHA11T, Pseudomonas baetica a390T, Pseudomonas iridis P42T, Pseudomonas atagonensis PS14T and Pseudomonas koreensis Ps 9-14T, within the Pseudom...

Background: Sterols are essential components of eukaryotic cells that modulate membrane biophysic... more Background: Sterols are essential components of eukaryotic cells that modulate membrane biophysical properties and function. Plants produce a complex mixture of sterols, among which β-sitosterol, stigmasterol, campesterol, and cholesterol in some Solanaceae, are the most abundant species. Many reports have shown that the stigmasterol to β-sitosterol ratio changes during plant development and in response to stresses, suggesting that it may play a role in the regulation of these processes. In tomato (Solanum lycopersicum), changes in the stigmasterol to β-sitosterol ratio correlate with the induction of the only gene encoding sterol C22-desaturase (C22DES), the enzyme specifically involved in the conversion of β-sitosterol to stigmasterol. However, despite the biological interest of this enzyme, there is still a lack of knowledge about several relevant aspects related to its structure and function.Results: In this study we report the subcellular localization of tomato C22DES in the en...

Different subcellular localization of arginine decarboxylase (ADC) transcripts and protein during mitosis in Scots pine zygotic embryos

Cloning of a Pinus sylvestris arginine decarboxylase gene and expression analysis in needles and root under potassium deficiency

Plant Biotechnology Journal, Apr 21, 2015

Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plan... more Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert b-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. b-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.

PLOS ONE, Oct 1, 2008

Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration... more Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.

Transcriptional upregulation of Pinus sylvestris arginine decarboxylase by potassium deficiency

International Journal of Systematic and Evolutionary Microbiology

In the framework of the research project called fitomatics, we have isolated and characterized a ... more In the framework of the research project called fitomatics, we have isolated and characterized a bacterial plant-endophyte from the rhizomes of Iris germanica, hereafter referred to as strain FIT81T. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase- and oxidase-positive. The optimal growth temperature of strain FIT81T is 28 °C, although it can grow within a temperature range of 4–32 °C. The pH growth tolerance ranges between pH 5 and 10, and it tolerates 4% (w/v) NaCl. A 16S rRNA phylogenetic analysis positioned strain FIT81T within the genus Pseudomonas , and multilocus sequence analysis revealed that Pseudomonas gozinkensis IzPS32dT, Pseudomonas glycinae MS586T, Pseudomonas allokribbensis IzPS23T, 'Pseudomonas kribbensis' 46–2 and Pseudomonas koreensis PS9-14T are the top five most closely related species, which were selected for further genome-to-genome comparisons, as well as for physiological and chemotaxonomic characterization. The g...

Plant Physiology, Mar 1, 1990

The common bean, Phaseolus vulgaris, contains a glycoprotein that inhibits the activity of mammal... more The common bean, Phaseolus vulgaris, contains a glycoprotein that inhibits the activity of mammalian and insect a-amylases, but not of plant a-amylases. It is therefore classified as an antifeedant or seed defense protein. In P. vulgaris cv Greensleeves, a-amy- lase inhibitor (aAI) is present in embryonic axes and cotyledons, but not in other organs of the plant. The protein is synthesized during the same time period that phaseolin and phytohemagglutinin are made and also accumulates in the protein storage vacuoles (protein bodies). Purified aAl can be resolved by SDS- PAGE into five bands (Mr 15,000-19,000), four of which have covalently attached glycans. These bands represent glycoforms of two different polypeptides. All the glycoforms have complex glycans that are resistant to removal by endoglycosidase H, indicating transport of the protein through the Golgi apparatus. The two different polypeptides correspond to the N-terminal and C-terminal halves of a lectin-like protein encoded by an already identified gene or a gene closely related to it (LM Hoffman [1984]

Frontiers in Plant Science, May 8, 2018

Tomato Sterol Acyltransferases transcriptional response to several stresses suggests a dual role ... more Tomato Sterol Acyltransferases transcriptional response to several stresses suggests a dual role of SlPSAT1 and SlASAT1 in tomato plant and fruit development and the adaptive responses to stress. Overall, this study contributes to enlarge the current knowledge on plant sterol acyltransferases and set the basis for further studies aimed at understanding the role of SE metabolism in tomato plant growth and development.

Resistance to Botrytis cinerea is induced in an Arabidopsis mutant with reduced levels of sterol glycosides

Trabajo presentado a la XXIII Reunion Bianual de la Sociedad Espanola de Fisiologia Vegetal y al ... more Trabajo presentado a la XXIII Reunion Bianual de la Sociedad Espanola de Fisiologia Vegetal y al XVI Congreso Hispano-Luso de Fisiologia Vegetal, celebrados en Pamplona (Espana) del 26 al 28 de junio de 2019.

Polyamine content and regulation during pre-penetration and penetration resistance events in oat against crown rust (Puccinia coronata f. sp. avenae)

CRISPR/Cas9-mediated inactivation of Sterol Acyltransferases and its effect in tomato plant growth and development

Trabajo presentado al Internal Seminar of the Centre de Recerca Agrigenómica (CRAG), celebrado el... more Trabajo presentado al Internal Seminar of the Centre de Recerca Agrigenómica (CRAG), celebrado el 8 de marzo de 2019

Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development

Environmental and Experimental Botany

Glycosylated sterols play an important role in tomato plant and fruit development

Trabajo presentado al XV Meeting of Plant Molecular Biology, celebrado online del 26 al 27 de nov... more Trabajo presentado al XV Meeting of Plant Molecular Biology, celebrado online del 26 al 27 de noviembre de 2020.Peer reviewe

Omics and Plant Abiotic Stress Tolerance

Trends in Plant Science, 1997

Polyamines are low molecular mass polycations found in all living organisms. In plants, they have... more Polyamines are low molecular mass polycations found in all living organisms. In plants, they have been implicated in a wide range of biological processes, including growth, development and stress responses. Recently, several key genes of the polyamine biosynthesis pathways have been cloned and shown to be developmentally regulated. Experiments in which the synthesis of polyamines has been perturbed have clearly shown that these compounds are essential for normal growth and development. These findings have been further supported by recent genetic analyses of polyamine synthesis mutants of Arabidopsis. The research has revealed a much more detailed level of understanding than hitherto, opening up new avenues of investigation for the future. p olyamines form a class of aliphatic amines that are ubiquitous in living organisms, and have been implicated in a wide range of biological processes, including plant growth and development. The major forms are putrescine (butan-l,4-diamine), spermidine [N-(3-aminopropyl) butane-l,4-diamine] and spermine [NN'-bis-(3aminopropyl) butane-l,4-diamine]. At cellular pH values, these compounds behave as cations, and can interact with

Proteomics: emerging tools to characterize plant metabolons

International Journal of Systematic and Evolutionary Microbiology

Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-... more Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28T, was isolated. FIT28T shows vigorous growth on nutrient rich media within the temperature range of 4–35 °C, with optimal growth at 28 °C, a wide pH tolerance from pH 5 to 11, and salt tolerance up to 6 % (w/v) NaCl. Colonies are white-yellow and quickly become mucoid. The results of analysis of the 16S rRNA gene sequence placed the strain within the genus Pseudomonas , and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB, gyrB and rpoD concatenated sequences revealed that the closest relatives of FIT28T are Pseudomonas zeae OE48.2T, ' Pseudomonas crudilactis ' UCMA 17988, Pseudomonas tensinigenes ZA5.3T, Pseudomonas helmanticensis OHA11T, Pseudomonas baetica a390T, Pseudomonas iridis P42T, Pseudomonas atagonensis PS14T and Pseudomonas koreensis Ps 9-14T, within the Pseudom...

Background: Sterols are essential components of eukaryotic cells that modulate membrane biophysic... more Background: Sterols are essential components of eukaryotic cells that modulate membrane biophysical properties and function. Plants produce a complex mixture of sterols, among which β-sitosterol, stigmasterol, campesterol, and cholesterol in some Solanaceae, are the most abundant species. Many reports have shown that the stigmasterol to β-sitosterol ratio changes during plant development and in response to stresses, suggesting that it may play a role in the regulation of these processes. In tomato (Solanum lycopersicum), changes in the stigmasterol to β-sitosterol ratio correlate with the induction of the only gene encoding sterol C22-desaturase (C22DES), the enzyme specifically involved in the conversion of β-sitosterol to stigmasterol. However, despite the biological interest of this enzyme, there is still a lack of knowledge about several relevant aspects related to its structure and function.Results: In this study we report the subcellular localization of tomato C22DES in the en...

Different subcellular localization of arginine decarboxylase (ADC) transcripts and protein during mitosis in Scots pine zygotic embryos

Cloning of a Pinus sylvestris arginine decarboxylase gene and expression analysis in needles and root under potassium deficiency

Plant Biotechnology Journal, Apr 21, 2015

Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plan... more Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert b-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. b-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.

PLOS ONE, Oct 1, 2008

Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration... more Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.

Transcriptional upregulation of Pinus sylvestris arginine decarboxylase by potassium deficiency