Dr Mohammad Alam Miah | Bangladesh Agricultural University (original) (raw)

Papers by Dr Mohammad Alam Miah

Journal of Advanced Veterinary and Animal Research, 2021

Objective: The study investigated the effect of different stocking density (SD) rates on the welf... more Objective: The study investigated the effect of different stocking density (SD) rates on the welfare, growth, and hemato-biochemical parameters in broiler chickens. Materials and Methods: 106 broiler chicks of 10 days old were used and assigned into four groups: A, B, C, and D. The chicks of group A were reared in floor space containing one bird per square foot area (SD1.0). The chicks of groups B, C, and D were reared at 1.5, 2.0, and 2.5 birds per square foot area (SD1.5, SD2.0, and SD2.5). Welfare, body weight, and hemato-biochemical parameters were assessed and monitored by physical observation and laboratory methods. Results: The birds reared at SD2.0, and SD2.5 rates showed increased panting breathing. Wet feces adhered below the vent. There were a significant number of birds showing dirtiness of body and feathers. Birds reared in SD2.5 were familiar with moist litters and high ammonia smell. Foot-pad dermatitis, scratches, and blister formation were detected in the leg. The s...

Bangladesh Journal of Veterinary Medicine, 2013

Microglia activation plays a pivotal role in varying stress conditions including oxidation, infla... more Microglia activation plays a pivotal role in varying stress conditions including oxidation, inflammation and certain neurodegenerative diseases. iNOS and PrxI are known to up regulate in LPS-activated microglia in vitro. Natural fruits including various berries containing both antioxidants and anti-inflammatory polyphenols may attenuate such stress conditions. We investigated the effects of blueberry extracts (BBE) on the LPS-activated stress related genes up regulation using BV-2 microglia cells in vitro. BV-2 cells were cultured in DMEM with 10% FBS, were pre-incubated with 50 µg/ml BBE for 0, 1 h and 12 h, and treated with 1µg/ml LPS for 24 h . The levels of mRNA and protein expression showed significant differences in iNOS and PrxI among the treatment groups. Hoechst and PI staining showed that BBE protects cells from activation induced death. Intracellular levels of ROS were increased by LPS stimulation over the level in the control cells whereas BBE treatment significantly low...

10 days old “Cobb 500” broiler chicks were used to investigate the influence of probiotics and gr... more 10 days old “Cobb 500” broiler chicks were used to investigate the influence of probiotics and growth promoter supplementation on growth performance and different hemato-biochemical parameters of broilers. They were randomly divided into three equal groups (n=10). Group A was considered as control, fed only with commercial ration and group B and group C were supplemented with probiotics and growth promoter respectively for the period of 21 days. Body weights were measured at 7 days interval and selective organ weights, total erythrocyte count(TEC), hemoglobin (Hb) concentration, packed cell volume (PCV), AST and ALT values were measured at the end of experiment. Results revealed that body weight was increased significantly (p 0.05) differences were found among groups of A, B, and C in the weight of breast meat, liver, Leg meat and spleen. All weights were increased significantly (p>0.05) both in probiotics and antibiotic growth promoter treated groups as compared to that of contr...

Nigella sativa (Black seed) has been recognized as one of the most popular herbs in many parts of... more Nigella sativa (Black seed) has been recognized as one of the most popular herbs in many parts of the world for centuries and used as folk medicine to cure different kinds of diseases. Vitamin E is well known for its antioxidative and anti-inflammatory properties, and has been studied in the prevention of cardiovascular disease (CVD). The present study was performed to determine the effect of black seed oil and Vit-E on growth performance and blood biochemistry in mice. A total of 40 mice weight between 25-27 gm were used for conducting the research. At first, the mice were randomly divided into 4 groups consisting 10 mice in each group. Group A served as the control group whereas Group B was administered black seed oil @ 0.5 ml/kg/day, Groups C was taken vitamin E @ 200 mg/kg/day and Group D was received both black seed oil @ 0.5 ml/kg/day + vitamin E @ 200 mg/kg/day respectively for 42 days. Result showed that, body weight of B, C and D group revealed a significant decrease than t...

Asian J. Med. Biol. Res., Jun 30, 2021

Asian J. Med. Biol. Res., Mar 31, 2021

The present study was carried out to investigate the haemato-biochemical profile during different... more The present study was carried out to investigate the haemato-biochemical profile during different stages of lactation in local Sahiwal crossbreed dairy cows at Savar area of Dhaka district of Bangladesh. Twenty four local Sahiwal crossbreed cows were selected randomly from different small farms and divided into three groups: early stage lactation (1-3 months), mid stage lactation (4-6 months) and late stage lactation (7-9 months). Blood samples were collected during three stages of lactation. There were no statistical significant (p<0.05) variations observed in hematological parameters amongst the three stage of lactation. Although the packed cell volume (PCV), hemoglobin (Hb) concentration and RBC count were found lowest in early stage of lactation than the other groups. Total leucocyte count (TLC), platelet count (PLT), mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were recorded within normal limits. Similarly, biochemical parameters were observed statist...

Objective: The present work was conducted on Cobb-500 broilers fed with either probiotics (Yogurt... more Objective: The present work was conducted on Cobb-500 broilers fed with either probiotics (Yogurt, Promax ®) or antibiotic growth promoters (Ciproflox ® , Renamycin ®) to assess the effects on body weight and biochemical parameters. Materials and methods: A total of 30 day-old broiler chicks were purchased and acclimatized for 7 days in the experimental shed. After initial acclimatization, the broilers were allotted into five equal groups (n=6). Group A was considered as control and was fed commercial ration and fresh drinking water. Groups B and C were fed commercial ration and probiotics (Yogurt, Promax ®) respectively mixed in water. Groups D and E received commercial ration and antibiotic growth promoters (Ciproflox ® , Renamycin ®) respectively with water. Body weight of each bird was recorded on day 7, 14, 21, 28 and 35. At the end of trial period, the birds were sacrificed to collect blood in order to prepare serum samples for biochemical analyses considering total cholesterol, triglyceride, high density lipids (HDL), creatinine, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Results: Body weight in all treatment groups (B, C, D and E) was significantly (P<0.05) higher as compared to control (A) group. Total cholesterol and triglyceride levels significantly (P<0.05) decreased in probiotics treated groups as compared to control and antibiotic growth promoters treated groups. AST and ALT values increased significantly (P<0.05) in antibiotic growth promoters treated groups as compared to control whereas, these values decreased in probiotics treated groups. Creatinine levels were significantly (P<0.05) higher in antibiotic growth promoters treated groups as compared to all others groups. Conclusion: Significantly increased body weight is observed in probiotics and antibiotic growth promoters supplemented broilers. Probiotics also improve the lipid profile and other biochemical parameters as compared to growth promoter. Probiotics (like Yogurt and Promax ®) seem to be better choice than antibiotic growth promoters as feed supplements.

Journal of Advanced Veterinary and Animal Research

Asian Journal of Dairy and Food Research

Background: The use of chromium (Cr) and vitamin E (Vit-E) to overcome the detrimental effects of... more Background: The use of chromium (Cr) and vitamin E (Vit-E) to overcome the detrimental effects of heat stress (HS) in broiler chickens has been studied by a few researchers. The study was designed to evaluate the effects of organic chromium picolinate (CrPic) and vit-E on physiological performances and immune responses in broiler chicken exposed to HS. Methods: A total of 120, day old chicks were reared and divided into four equal groups: A, B, C and D at day 13. Group A considered as non-treated control, Group B, C and D were treated with CrPic, Vit-E and both respectively. The birds were exposed heat to 35 ± 3°C for 6 hours for 22 days.Result: CrPic, Vit-E and combined groups had significantly higher live body weight and lower FCR (p less than 0.05). Hematological values were not varied among the groups. Liver enzymes AST but not ALT were increased significantly in the Vit-E group. Creatinine level was significantly (p less than 0.05) higher in CrPic and combined groups. Total pro...

Asian Journal of Medical and Biological Research

Bisphenol-A (BPA) is one of the highest volume chemicals produced world-wide and used in the manu... more Bisphenol-A (BPA) is one of the highest volume chemicals produced world-wide and used in the manufacture of plastics and epoxy resins that are pervasive in our environment and daily lives. The present research was carried out to investigate the effects of two different doses of Bisphenol-A (BPA) on the body weight, hematological parameters and patho-physiological changes of kidney in mice. For this study, fifteen mice, 6 to 8 weeks of age with an average bwt 27.10±0.5 gm, were randomly divided into three groups (n= 5). Group A (control) received only normal mouse pellet while group B and group C received pellet mixed with BPA @ 50 mg and 100 mg / kg bwt daily for 12 weeks, respectively. At the end of the experiment, blood and tissues were collected and processed for hematological and histopathological examination. Results showed that BPA- treated mice caused significant elevation (p<0.01) in weight gain even treated with low dose (50mg) of BPA. The mice exposed to high dose of BP...

Journal of the Bangladesh Agricultural University

Long-term intake of high-fat food disrupts lipid metabolism, resulting in fat accumulation, obesi... more Long-term intake of high-fat food disrupts lipid metabolism, resulting in fat accumulation, obesity, and hyperlipidemia. Curcumin, active ingredients of turmeric plants, has been shown to possess potent antioxidant and hypolipidemic properties. The study was conducted to observe the effects of curcumin on butter induced hyperlipidemia, hematological values and physio-pathological alterations in liver and aorta in mice. A total of 40 Swiss Albino mice (Mus musculus), aged 28-30 days with an average body weight of 27g were randomly divided into 2 groups. Group A was considered as non-treated control (NT) (n=8) and fed on standard mice pellet and fresh drinking water. Another group was considered as butter group (n=32), fed on standard mice pellet enriched with 10% butter and after 5 weeks, mice of butter group were subdivided into 4 equal groups (n=8). Group B was considered as butter treated (BT) group fed on standard mice pellet enriched with 10% butter. Group C (C1%), group D (C2%)...

The Journal of Immunology

Ischemic myocardial injury results in sterile cardiac inflammation that leads to tissue repair, t... more Ischemic myocardial injury results in sterile cardiac inflammation that leads to tissue repair, two processes controlled by mononuclear phagocytes. Despite global burden of cardiovascular diseases, we do not understand the functional contribution to pathogenesis of specific cardiac mononuclear phagocyte lineages, in particular dendritic cells. To address this limitation, we used detailed lineage tracing and genetic studies to identify bona fide murine and human CD103 + conventional dendritic cell (cDC)1s, CD11b + cDC2s, and plasmacytoid DCs (pDCs) in the heart of normal mice and immunocompromised NSG mice reconstituted with human CD34 + cells, respectively. After myocardial infarction (MI), the specific depletion of cDCs, but not pDCs, improved cardiac function and prevented adverse cardiac remodeling. Our results showed that fractional shortening measured after MI was not influenced by the absence of pDCs. Interestingly, however, depletion of cDCs significantly improved reduction in fractional shortening. Moreover, fibrosis and cell areas were reduced in infarcted zones. This correlated with reduced numbers of cardiac macrophages, neutrophils, and T cells, indicating a blunted inflammatory response. Accordingly, mRNA levels of proinflammatory cytokines IL-1b and IFN-g were reduced. Collectively, our results demonstrate the unequivocal pathological role of cDCs following MI.

Bangladesh Journal of Veterinary Medicine

The effect of different levels of supplemented dietary protein on body weight, certain haematolog... more The effect of different levels of supplemented dietary protein on body weight, certain haematological values and meat yield characteristics in 20 "Shaver Star Bro" broilers was studied during the period from 02 March to 12 April 2002. The broilers were randomly assigned to four equal groups (A to D) each consisting of 5 birds. Group A was considered as control, fed only with commercial ration and other three groups were treated with extra dietary protein supplement @ 5%, 10% and 15% as group B, C and D respectively. Increased body weight was recorded in group B and the body weight decreases as the level of protein increased from 5% to 10% and 15% and the decrease was linear. No differences in haematological values were observed among the groups but the TEC varied significantly (p < 0.05). The mean weight of breast meat, drum stick meat varied significantly (p < 0.01). The shank weight varied significantly (p < 0.05) but there was no significant difference among th...

한국발생생물학회 2008년도 전기 제26차 학술대회 초록, May 1, 2008

Cell Metabolism, 2016

Autoimmune diseases (AIDs), a heterogeneous group of immune-mediated disorders, are a major and g... more Autoimmune diseases (AIDs), a heterogeneous group of immune-mediated disorders, are a major and growing health problem. Although AIDs are currently treated primarily with anti-inflammatory and immunosuppressive drugs, the use of stem cell transplantation in patients with AIDs is becoming increasingly common. However, stem cell transplantation therapy has limitations, including a shortage of available stem cells and immune rejection of cells from nonautologous sources. Induced pluripotent stem cell (iPSC) technology, which allows the generation of patient-specific pluripotent stem cells, could offer an alternative source for clinical applications of stem cell therapies in AID patients. We used nonintegrating oriP/EBNA-1-based episomal vectors to reprogram dermal fibroblasts from patients with AIDs such as ankylosing spondylitis (AS), Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE). The pluripotency and multilineage differentiation capacity of each patient-specific iPSC line was validated. The safety of these iPSCs for use in stem cell transplantation is indicated by the fact that all AID-specific iPSCs are integrated transgene free. Finally, all AID-specific iPSCs derived in this study could be differentiated into cells of hematopoietic and mesenchymal lineages in vitro as shown by flow cytometric analysis and induction of terminal differentiation potential. Our results demonstrate the successful generation of integration-free iPSCs from patients with AS, SS and SLE. These findings support the possibility of using iPSC technology in autologous and allogeneic cell replacement therapy for various AIDs, including AS, SS and SLE.

한국발생생물학회 학술발표대회, May 1, 2008

A total of 25. one month old Long Evan male rats were studied to observe the effects of different... more A total of 25. one month old Long Evan male rats were studied to observe the effects of different edible oils on hemato-biochemical profiles. They were randomly assigned to one of five equal groups (n= 5) as A, B, C, D and E. Group. A was considered as control fed with rat feed (ICDDR`B) and others were supplemented with soybean oil (Group B), palm oil (Group C), coconut oil (Group D) and mustard oil (Group E) at a concentration of 7.5% with rat feed for 8 weeks. TEC was increased significantly (P0.05) among treatment groups compared to control. TLC differed insignificantly (P>0.05) in group B,C,D but varied significantly (P0.05) in the treatment groups. Triglycerides concentration of group E differed significantly (P0.05) difference of HDL-cholesterol in treatment groups with the control. The LDL- cholesterol values were below detection level in all rats. The results suggest that edible oils have little effect in rat haematobiochemical parameters. Further research should be carr...

Bangladesh Journal of Veterinary Medicine, 2012

The effects of different edible oils on body weight gain, different organ weight and serum transa... more The effects of different edible oils on body weight gain, different organ weight and serum transaminases were studied on 25, one month old long Evans male rats during the period from 3 April to 28 May 2003. The rats were randomly assigned to one of five equal groups (n = 5) as A, B, C, D and E of which group A was considered as control and fed with rat pellets (ICDDR, B) and others were supplemented with oils as soybean (group B), palm (group C, coconut (group D) and mustard (group E) at a concentration of 7.5% with pellets for 8 weeks. The results revealed significant (p < 0.001) increase in weight gain of the non supplemented rats (control). The mean heart weight of the nonsupplemented rats (control) was significantly (p < 0.05) higher than those of the treated groups. The mean liver weight of nonsupplemented, coconut and mustard oils treated rats were significantly (p < 0.05) higher than those of soybean and palm oil treated groups. The mean kidney weight of the mustard ...