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Papers by Sharif Ar-Raffi

Research paper thumbnail of Genetic variability, heritability and genetic advance of yield and yield contributing characters of lablab bean (Lablab purpureus L.)

Studies on genetic variability, heritability and genetic advance were carried out with 14 genotyp... more Studies on genetic variability, heritability and genetic advance were carried out with 14 genotypes of Lablab bean (Lablab purpureus L.) cultivated at the Agro-ecological zone9 (AEZ-9) comprising the Old Brahmaputra Floodplain Soil. Considerable amount of genotypic and phenotypic coefficient of variation was observed for all characters studied. Phenotypic variation was greater than that of the genotypic variations for all the characters. The greater portion of total phenotypic variation was due to the genotypic variation. Highest genetic and phenotypic variation were observed for green pod yield per plant (1882.68 and 2108.43, respectively) and 100-green seed weight (1380.91 and 1452.07, respectively) Heritability values were higher for days to maturity (81.37) , number of raceme per plant (80.62), pod length (99.43), pod width (99.27), green pod yield per plant (89.29), dry shelling percentage (91.58), seed yield per plant (84.93) and protein content (98.11) indicating the better potentials of improving these characters for improvement of yield. Maximum genetic advance expressed as percentage of mean was recorded for green pod yield per plant (95.22).

Research paper thumbnail of CHARACTER ASSOCIATION AND PATH COEFFICIENT ANALYSIS OF GRAIN YIELD AND YIELD RELATED TRAITS IN SOME PROMISING EARLY TO MEDIUM DURATION RICE ADVANCED LINES

Evaluation of advanced lines is an important step in any crop improvement program. A field experi... more Evaluation of advanced lines is an important step in any crop improvement program. A field experiment was
conducted using twenty three rice genotypes including three check varieties during the period from June to December
2013 at the Agronomy Field Laboratory, Department of Agronomy, Bangladesh Agricultural University,
Mymensingh to investigate the association among eight morpho-physiological traits including yield. The experiment
was laid out in a randomized complete block design with three replications. Grain yield was found to be positively
and significantly correlated with filled grain per panicle, plant height, days to 50% flowering and days to maturity
both at genotypic and phenotypic levels, indicating the importance of these traits for yield improvement in rice. From
the path coefficient analysis, it was revealed that days to maturity, days to 50% flowering, plant height, number of
filled grain per panicle and grain length had direct positive effect on yield, indicating these are the main contributors
to yield. Eventually, it was recommended that, for obtaining increased rice yield, a genotype should possess more number of filled grains per panicle.

Research paper thumbnail of Analysis of genetic variability, heritability and genetic advance for yield and yield associated traits in some promising advanced lines of rice

A field experiment was conducted using twenty three rice genotypes including three check varietie... more A field experiment was conducted using twenty three rice genotypes including three check varieties during the period from June to December, 2013, at the Agronomy Field Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh to study genetic variability, heritability and genetic advance (GA) for yield and yield associated traits in rice. The experiment was laid down in a randomized complete block design with three replications. Plant height, number of filled grains per panicle, days to 50% flowering, thousand grain weight, grain width and grain yield showed relatively high genotypic co-efficient of variation (GCV) and phenotypic co-efficient of variation (PCV) estimates. The PCV were higher than GCV for all eight traits indicating that they all interacted with the environment to some extent. High heritability was obtained for grain width (88.54%), followed by days to 50% flowering (87.61%), thousand grain weight (81.96%), grain length (81.94%), days to maturity (81.81%) and number of filled grains per panicle (78.19%) which indicates high heritable portion of variation. High to medium estimates of heritability and genetic advance were obtained for number of filled grains per panicle (18.97), days to 50% flowering (11.89), days to maturity (12.16) indicating the roles of additive gene action and a good scope of selection using their phenotypic performance. Considering, all of these characters, filled gains per panicle and days to 50% flowering and maturity were important yield related traits and could be used for selection in rice breeding programs.

Research paper thumbnail of Morphological and molecular characterization of some NERICA mutant lines under drought condition

To ensure the productivity of rice in drought condition, ‘NERICA’ varieties of African origin has... more To ensure the productivity of rice in drought condition, ‘NERICA’ varieties of African origin has been introduced in Bangladesh and subsequently mutant lines are also developed for improving the performance further. The present study was undertaken to screen out better drought tolerant mutant lines of NERICA having other desirable traits. Thirteen NARICA mutant lines, four susceptible (BINA dhan8, BINA dhan10, BINA dhan11 and BINA dhan12) and one tolerant (BRRI dhan56) varieties were included in the experiment and selection was based on morphological and molecular characteristics. The plants were grown in pot containing 9 kg of soil and when they were 60 days old, drought was imposed by adding 1L of distilled water at every four days interval till harvesting. The quantity of water applied to control pots was double. The results showed that important yield contributing morphological attributes like days of 50% flowering, plant height, total number of tiller hill-1, panicle length, 100 seed weight, fresh weight of plant and dry weight of plant were significantly positively regulated due to drought in N10/300/P-2(1)-3-5, N1/250/P-6-2-7 and N10/300/P-2(1)-6-13 mutant lines. The findings of genetic diversity analysis through SSR markers RM32, RM202, RM351 and RM510 for N10/300/P-2(1)-3-5, N1/350/P-2-2-6 and N1/250/P-6-2-7 lines appeared to resemble to that of control (BRRI dhan56) from the UPGMA dendrogram. Based on morphological, molecular characteristics and UPGMA dendrogram study, mutant lines N10/300/P-2(1)-3-5 and N1/250/P-6-2-7 lines appeared superior as genetic material for developing better drought tolerant rice varieties.

Research paper thumbnail of CORRELATION AND PATH COEFFICIENT ANALYSIS OF FOURTEEN DIFFERENT GENOTYPES OF LABLAB BEAN (Lablab purpureus L.)

Bangladesh Journal of Plant Breeding and Genetics, 2015

Research paper thumbnail of Somatic Embryo Formation from Co-cultivated Protoplasts of Brassica rapa & B. juncea

American Journal of Experimental Agriculture, 2015

Research paper thumbnail of Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis

Photosynthetic light energy utilisation is accompanied with the pH-gradient formation, O 2 produc... more Photosynthetic light energy utilisation is accompanied with the pH-gradient formation, O 2 production, CO 2 fixation, heat propagation and other processes, which result in dynamic changes in a volume of chloroplasts, whole cells or plant tissue. Here, we present a new concept, in which the volume changes in chloroplasts, photosynthetic bacteria, and plant leaves are measured using interference of coherent light beams produced by a HeNe laser (λ 632.8 nm). The aim and main advantage of this interferometric method applied newly to photosynthesis research is the possibility to quantify the non-photochemical processes in photosynthetic samples measured in vivo using the parallel recording of interferograms and chlorophyll a fluorescence induction kinetics. In this way, the heat production in bacteria (Rhodobacter sphaeroides) treated with a gramicidin, as well as the O 2 evolution, CO 2 uptake and transverse dilatations in leaves of bean (Phaseolus vulgaris) stressed by the excessive radiation were studied. We found that the heat production was enhanced by approximately 20% in stressed samples. Using interferometry, we have also observed pronounced transverse dilatations in a leaf tissue of bean during photosynthesis caused probably by transpiration.

Research paper thumbnail of Functional characterization of a T-DNA inserted STN8 kinase mutant of Rice

Research paper thumbnail of Chromosomes responsible for salt tolerance in Hordeum marinum as expressed in a wheat background

Post Graduate Conference, 2010

Welcome! We hope you all get a lot out of this conference. As you know this is the first time we ... more Welcome! We hope you all get a lot out of this conference. As you know this is the first time we have put together student papers as part of the conference. We are very pleased with your result with 36 papers being submitted. For the student presentations we have organised expert panels to discuss your work in a supportive atmosphere. We are grateful to Graham Gardner who shared his very successful format for postgraduate meetings that he runs for the Beef and Sheep CRCs. In the spirit of what we hope is a growing linkage between education managers of CRCs, we have invited along Belinda Griffiths, from the newly established Dairy CRC, so that she can experience firsthand a postgraduate student conference. We hope that the success of our, and other conferences, will be replicated across the CRCs! Of course a conference takes a lot of organisation and we are indebted to the following for helping bring things together. To Kamala Anggamuthu, our administrator extraordinaire who organised, flights, meals…and us!; Jodi Mitchell for organising the accommodation and conference dinner; Sarah Colombera and Barbara Williams at FFI headquarters for their organisational skills and for answering our stream of questions; to Christine Davies for her miraculous editing and tweaking skills; the willing panellists, speakers, Ryun Fell from CSU Learning and Teaching Services for organising videotaping of talks and other CSU staff who have been very willing to lend a hand.

Research paper thumbnail of Stability Analysis for Pod and Seed Production in Dry Bean (Phaseolus vulgaris L.)

Asian Journal of Plant Sciences, 2004

Research paper thumbnail of Genetic variability, heritability and genetic advance of yield and yield contributing characters of lablab bean (Lablab purpureus L.)

Studies on genetic variability, heritability and genetic advance were carried out with 14 genotyp... more Studies on genetic variability, heritability and genetic advance were carried out with 14 genotypes of Lablab bean (Lablab purpureus L.) cultivated at the Agro-ecological zone9 (AEZ-9) comprising the Old Brahmaputra Floodplain Soil. Considerable amount of genotypic and phenotypic coefficient of variation was observed for all characters studied. Phenotypic variation was greater than that of the genotypic variations for all the characters. The greater portion of total phenotypic variation was due to the genotypic variation. Highest genetic and phenotypic variation were observed for green pod yield per plant (1882.68 and 2108.43, respectively) and 100-green seed weight (1380.91 and 1452.07, respectively) Heritability values were higher for days to maturity (81.37) , number of raceme per plant (80.62), pod length (99.43), pod width (99.27), green pod yield per plant (89.29), dry shelling percentage (91.58), seed yield per plant (84.93) and protein content (98.11) indicating the better potentials of improving these characters for improvement of yield. Maximum genetic advance expressed as percentage of mean was recorded for green pod yield per plant (95.22).

Research paper thumbnail of CHARACTER ASSOCIATION AND PATH COEFFICIENT ANALYSIS OF GRAIN YIELD AND YIELD RELATED TRAITS IN SOME PROMISING EARLY TO MEDIUM DURATION RICE ADVANCED LINES

Evaluation of advanced lines is an important step in any crop improvement program. A field experi... more Evaluation of advanced lines is an important step in any crop improvement program. A field experiment was
conducted using twenty three rice genotypes including three check varieties during the period from June to December
2013 at the Agronomy Field Laboratory, Department of Agronomy, Bangladesh Agricultural University,
Mymensingh to investigate the association among eight morpho-physiological traits including yield. The experiment
was laid out in a randomized complete block design with three replications. Grain yield was found to be positively
and significantly correlated with filled grain per panicle, plant height, days to 50% flowering and days to maturity
both at genotypic and phenotypic levels, indicating the importance of these traits for yield improvement in rice. From
the path coefficient analysis, it was revealed that days to maturity, days to 50% flowering, plant height, number of
filled grain per panicle and grain length had direct positive effect on yield, indicating these are the main contributors
to yield. Eventually, it was recommended that, for obtaining increased rice yield, a genotype should possess more number of filled grains per panicle.

Research paper thumbnail of Analysis of genetic variability, heritability and genetic advance for yield and yield associated traits in some promising advanced lines of rice

A field experiment was conducted using twenty three rice genotypes including three check varietie... more A field experiment was conducted using twenty three rice genotypes including three check varieties during the period from June to December, 2013, at the Agronomy Field Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh to study genetic variability, heritability and genetic advance (GA) for yield and yield associated traits in rice. The experiment was laid down in a randomized complete block design with three replications. Plant height, number of filled grains per panicle, days to 50% flowering, thousand grain weight, grain width and grain yield showed relatively high genotypic co-efficient of variation (GCV) and phenotypic co-efficient of variation (PCV) estimates. The PCV were higher than GCV for all eight traits indicating that they all interacted with the environment to some extent. High heritability was obtained for grain width (88.54%), followed by days to 50% flowering (87.61%), thousand grain weight (81.96%), grain length (81.94%), days to maturity (81.81%) and number of filled grains per panicle (78.19%) which indicates high heritable portion of variation. High to medium estimates of heritability and genetic advance were obtained for number of filled grains per panicle (18.97), days to 50% flowering (11.89), days to maturity (12.16) indicating the roles of additive gene action and a good scope of selection using their phenotypic performance. Considering, all of these characters, filled gains per panicle and days to 50% flowering and maturity were important yield related traits and could be used for selection in rice breeding programs.

Research paper thumbnail of Morphological and molecular characterization of some NERICA mutant lines under drought condition

To ensure the productivity of rice in drought condition, ‘NERICA’ varieties of African origin has... more To ensure the productivity of rice in drought condition, ‘NERICA’ varieties of African origin has been introduced in Bangladesh and subsequently mutant lines are also developed for improving the performance further. The present study was undertaken to screen out better drought tolerant mutant lines of NERICA having other desirable traits. Thirteen NARICA mutant lines, four susceptible (BINA dhan8, BINA dhan10, BINA dhan11 and BINA dhan12) and one tolerant (BRRI dhan56) varieties were included in the experiment and selection was based on morphological and molecular characteristics. The plants were grown in pot containing 9 kg of soil and when they were 60 days old, drought was imposed by adding 1L of distilled water at every four days interval till harvesting. The quantity of water applied to control pots was double. The results showed that important yield contributing morphological attributes like days of 50% flowering, plant height, total number of tiller hill-1, panicle length, 100 seed weight, fresh weight of plant and dry weight of plant were significantly positively regulated due to drought in N10/300/P-2(1)-3-5, N1/250/P-6-2-7 and N10/300/P-2(1)-6-13 mutant lines. The findings of genetic diversity analysis through SSR markers RM32, RM202, RM351 and RM510 for N10/300/P-2(1)-3-5, N1/350/P-2-2-6 and N1/250/P-6-2-7 lines appeared to resemble to that of control (BRRI dhan56) from the UPGMA dendrogram. Based on morphological, molecular characteristics and UPGMA dendrogram study, mutant lines N10/300/P-2(1)-3-5 and N1/250/P-6-2-7 lines appeared superior as genetic material for developing better drought tolerant rice varieties.

Research paper thumbnail of CORRELATION AND PATH COEFFICIENT ANALYSIS OF FOURTEEN DIFFERENT GENOTYPES OF LABLAB BEAN (Lablab purpureus L.)

Bangladesh Journal of Plant Breeding and Genetics, 2015

Research paper thumbnail of Somatic Embryo Formation from Co-cultivated Protoplasts of Brassica rapa & B. juncea

American Journal of Experimental Agriculture, 2015

Research paper thumbnail of Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis

Photosynthetic light energy utilisation is accompanied with the pH-gradient formation, O 2 produc... more Photosynthetic light energy utilisation is accompanied with the pH-gradient formation, O 2 production, CO 2 fixation, heat propagation and other processes, which result in dynamic changes in a volume of chloroplasts, whole cells or plant tissue. Here, we present a new concept, in which the volume changes in chloroplasts, photosynthetic bacteria, and plant leaves are measured using interference of coherent light beams produced by a HeNe laser (λ 632.8 nm). The aim and main advantage of this interferometric method applied newly to photosynthesis research is the possibility to quantify the non-photochemical processes in photosynthetic samples measured in vivo using the parallel recording of interferograms and chlorophyll a fluorescence induction kinetics. In this way, the heat production in bacteria (Rhodobacter sphaeroides) treated with a gramicidin, as well as the O 2 evolution, CO 2 uptake and transverse dilatations in leaves of bean (Phaseolus vulgaris) stressed by the excessive radiation were studied. We found that the heat production was enhanced by approximately 20% in stressed samples. Using interferometry, we have also observed pronounced transverse dilatations in a leaf tissue of bean during photosynthesis caused probably by transpiration.

Research paper thumbnail of Functional characterization of a T-DNA inserted STN8 kinase mutant of Rice

Research paper thumbnail of Chromosomes responsible for salt tolerance in Hordeum marinum as expressed in a wheat background

Post Graduate Conference, 2010

Welcome! We hope you all get a lot out of this conference. As you know this is the first time we ... more Welcome! We hope you all get a lot out of this conference. As you know this is the first time we have put together student papers as part of the conference. We are very pleased with your result with 36 papers being submitted. For the student presentations we have organised expert panels to discuss your work in a supportive atmosphere. We are grateful to Graham Gardner who shared his very successful format for postgraduate meetings that he runs for the Beef and Sheep CRCs. In the spirit of what we hope is a growing linkage between education managers of CRCs, we have invited along Belinda Griffiths, from the newly established Dairy CRC, so that she can experience firsthand a postgraduate student conference. We hope that the success of our, and other conferences, will be replicated across the CRCs! Of course a conference takes a lot of organisation and we are indebted to the following for helping bring things together. To Kamala Anggamuthu, our administrator extraordinaire who organised, flights, meals…and us!; Jodi Mitchell for organising the accommodation and conference dinner; Sarah Colombera and Barbara Williams at FFI headquarters for their organisational skills and for answering our stream of questions; to Christine Davies for her miraculous editing and tweaking skills; the willing panellists, speakers, Ryun Fell from CSU Learning and Teaching Services for organising videotaping of talks and other CSU staff who have been very willing to lend a hand.

Research paper thumbnail of Stability Analysis for Pod and Seed Production in Dry Bean (Phaseolus vulgaris L.)

Asian Journal of Plant Sciences, 2004