Sumitra Saha | Bangladesh Agricultural University (original) (raw)

Papers by Sumitra Saha

SN Business & Economics, Jan 31, 2023

South Asian Survey, Sep 1, 2022

BioTechnologia

Aloe vera [Aloe vera (L.) Burm. f.] is considered a valuable medicinal plant worldwide due to its... more Aloe vera [Aloe vera (L.) Burm. f.] is considered a valuable medicinal plant worldwide due to its remarkable beneficial effects on human health. However, challenges in A. vera propagation hinder meeting the increasing demand in the health and beauty sectors. As an alternative method, in vitro propagation is crucial for the mass production of Aloe plants, which is a rapid method as well. Therefore, the present study aimed to establish an efficient micropropagation protocol for A. vera by in vitro optimization of the effect of different plant growth regulators (PGRs). For shoot proliferation, sterilized explants were inoculated on the Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and thidiazuron (0.5, 1.0, 2.0, and 4.0 mg/l) in combination with 0.5 mg/l naphthaleneacetic acid (NAA). Subsequently, indole-3-butyric acid (IBA) (1.0, 2.0, and 3.0 mg/l) was used for root induction. It was found that the explants cultured on the MS medium supplemented with 4.0 mg/l BAP + 0.5 mg/l NAA showed the highest percentage of response (90 ± 1.29) for shoot induction within the minimum number of days (5 ± 0.33). The highest number of shoots (2.7 ± 0.36) and length of shoots (4.7 ± 0.42 cm) per explant were also observed with the same concentration of PGRs. However, the highest number of roots (3.2 ± 0.57), length of roots (5.67 ± 0.21 cm), and root induction (80 ± 1.97 %) were noticed within the minimum number of days (11 ± 0.79) on the MS medium supplemented with 1.0 mg/l IBA. Thus, the proposed method is a quick and effective approach for the mass propagation of A. vera with appropriate dosages of auxins and cytokinins, which may allow meeting the increasing commercial demand.

Journal of Bangladesh Agricultural University

The alarming trend of the resistance in microbes drives researchers to re-evaluate the existing m... more The alarming trend of the resistance in microbes drives researchers to re-evaluate the existing materials and methods used to maintain aseptic conditions in laboratories. Therefore, this study was designed to better understand surface microbes in biotechnology laboratory areas that may develop resistance and the action needed to control the transmission by re-evaluating the efficiency of commonly used surface sterilant: 70% ethanol. For this purpose, surface swabs were collected from the four commonly used uncleaned working spots: media preparation area, molecular area, inoculation area, and incubation area. Pure colonies were characterised morphologically and biochemically using gram staining, catalase, oxidase, indole, urase, and gelatin tests. According to morphological and biochemical characteristics, the representative bacteria were concluded as Bacillus spp., from media preparation and molecular area, Staphylococcus spp., and Pseudomonas spp, from inoculation and incubation ar...

Saudi Journal of Biological Sciences

Bioresource Technology Reports, 2022

Bangladesh Journal of Animal Science, 2012

The present research was undertaken to explore the maturation of buffalo oocytes using bovine fol... more The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilize...

Fighting against global hunger in the adverse climatic condition is a major concern of the govern... more Fighting against global hunger in the adverse climatic condition is a major concern of the governments around the globe. The pace of population growth is overwhelmingly defeating the growth in crop production. In this context, introduction of GMO is emerging as a probable solution for sustainable crop production. However, such developments are not beyond criticism. This paper assesses and evaluates the prospects and challenges of introduction of GMO in a global perspective. Experiences of GM crop cultivation in different countries are also considered. This paper is a review of all accessible literature on GMO and sustainable crop production. This review will give a flavor of the on going debate and help take decision by the interested countries regarding GMO adoption. Furthermore, on the basis of the review this paper suggests some policy recommendations.

Agricultural Science, 2020

This study was conducted to assess the effect of DCP and vitamin E supplementation on body weight... more This study was conducted to assess the effect of DCP and vitamin E supplementation on body weight, hematological(TEC, Hb content, PCV and ESR) and biochemical (AST and ALT) parameters of “Cobb 500” broiler. A total of 20 chicks (14 days old) were reared and randomly divided into four equal groups (n=5). Body weight was measured at each 7 days interval up to the end of the 35 days of experimental period. Group A was considered as control, fed with commercial ration. Group B, C and D were treated with 1 gm DCP per kg feed, 1 ml vitamin E per litre of drinking water, and 1 gm DCP per kg feed plus 1 ml vitamin E per litre of drinking water with commercial ration, respectively from day 14 to day 35. It was observed that DCP and vitamin E supplementation significantly (p<0.01) increased body weight. Moreover, TEC and Hb content increased significantly (p<0.01) in the treated groups as compared to control. Besides, ESR, AST and ALT values decreased significantly (p<0.01) in the tr...

Journal of The Bangladesh Agricultural University, 2020

Journal of Bangladesh Agricultural University, 2021

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in v... more This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II stage was 61.41 ± 1.97%. The matured COCs were fertilized for 5 hours in Brackett and Oliphant media using fresh and frozen thawed semen separately. After fertilization the oocytes were cultured in TCM-199 for 48 hours to observe the cleavage rate. The maturation, fertilization and culture were performed in an incubator at 38.5 ˚C with 5% CO 2 in humidified air. After fertilization cleavage rates were observed to check the fitness of zygotes to be morula and blastocyst. It was observed that the rates of normal fertilization (2 PN formations) for fresh and frozen semen were 36.02 ± 2.79 and 34.73 ± 2.58%, respectively and the cleavage rates were 25.19 ± 2.5 and 21.01 ± 2.8%, respectively. No significant differences (P>0.05) was observed between fresh and frozen semen in the efficiency of in vitro fertilization and subsequent development of goat embryos. It can be concluded that, both fresh and frozen semen can be used for IVF and subsequent development of goat embryos.

Journal of the Bangladesh Agricultural University, Dec 31, 2019

The present study was conducted to analyze genetic diversity among biodiesel producing plant Jatr... more The present study was conducted to analyze genetic diversity among biodiesel producing plant Jatropha curcas genotypes using six RAPD primers. The J. curcas samples were collected from ten agro climatic regions in Bangladesh. The six primers produced 31 DNA bands. All the DNA bands (31) showed 100% polymorphism. Overall gene frequency of 10 genotypes of J. curcas was ranged from 0.10 to 0.90. The average Nei's gene diversity and Shannon's Information Index for all loci were estimated 0.2994 and 0.4650, respectively. The gene diversity value was ranged from 0.18 to 0.50 and Shannon's Information Index was ranged from 0.3251 to 0.6931. Inter-germplasm similarity indices (Si) ranged from 5.56 to 66.67% with an average of 33.906%. The similarity coefficient range varied from 0.00 to 0.971. The UPGMA dendrogram constructed from Nei's (1972) genetic distance group indicated segregation of the ten J. curcas germplasms into three main clusters. Cluster I, II and III possessed 8, 1 and 1 germplasms, respectively. This study revealed that at least three different J. curcas genotypes are available in Bangladesh. The RAPD technique is, however, found to be useful in studying genetic variation among J. curcas genotypes of different regions in Bangladesh.

Ever since the birth of the first buffalo calf following in vitro fertilization (1), in vitro pro... more Ever since the birth of the first buffalo calf following in vitro fertilization (1), in vitro production of buffalo embryos has been gaining attention for its research and commercial applications. Progress in the use of embryo transfer in the buffalo is slow due to poor superovulatory response. ...

Journal of the Bangladesh Agricultural University, 2013