puspita deswina | Indonesian Institute of Sciences (original) (raw)

Papers by puspita deswina

Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto, Jul 20, 2020

Improving the quality of rice crops is the first priority to reach the high productivity and food... more Improving the quality of rice crops is the first priority to reach the high productivity and food security in Indonesia. Food needs and nutritional adequacy of the community can be met if the quality of rice increases, so the goal of food self-sufficiency can be realized. The policy of importing rice should immediately end by providing premium-quality rice so that major food needs can be provided. Rice production in Indonesia should be continuously improved to balance with the increasing of growth population every year. Various efforts have been made such as improving the quality of rice to provide better results. The research objectives were selection and evaluation of rice varieties through participatory plant breeding with rice field farmer respondents. Rice farmers from Cibinong, Bogor and surrounding areas were asked to provide an assessment of the morphological characteristics of cross rice crops. The research objectives were selection and evaluation of rice varieties through participatory plant breeding with rice field farmer respondents. Rice farmers from Cibinong, Bogor and surrounding areas were asked to provide an assessment of the morphological characteristics of cross rice crops. The highest assessment result was P3 strain (402) selected by 15 farmers, then P1 (275) selected by 11 farmers. The most preferred varieties of rice are Limboto and IR 64.

Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry... more Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry (crylB-cry\ Aa) dan cry IB dengan promoter terinduksi pelukaan (mpi), teiah didapat. Fusi dua gen cry dan penggunaan promoter terinduksi pelukaan (mpi) ditujukan ...

Al-Kauniyah: Jurnal Biologi

AbstrakPengujian stabilitas genotip merupakan hal yang penting dalam perakitan varietas padi gog... more AbstrakPengujian stabilitas genotip merupakan hal yang penting dalam perakitan varietas padi gogo. Metode pengujian yang sering dilakukan menggunakan analisis ANOVA gabungan yang harus memenuhi normalitas data dan homogenitas ragam. Terkadang data yang diperoleh tidak dapat memenuhi asumsi, sehingga diperlukan analisis alternatif menggunakan metode kualitatif, yaitu Additive Main Effect and Main Interaction (AMMI). Penelitian menggunakan empat belas genotip padi gogo pada tujuh lokasi, dengan rancangan percobaan rancangan acak kelompok. Hasil analisis AMMI1 menunjukkan bahwa didapatkan tujuh genotip dengan nilai rerata produksi di atas rata-rata umum yaitu G29, G31, G33, G34, G35, G37, dan G42 (Inpago 9). Inpago 9 sebagai genotip dengan rata-rata produksi tertinggi di seluruh lokasi. Hasil analisis AMMI2 didapatkan genotip dengan kemampuan adaptasi spesifik lokasi dan beradaptasi pada seluruh lokasi. Dua genotip beradaptasi baik di lokasi Probolinggo yaitu G39 dan G40, sedangkan G2...

GMO Biosafety Research, 2015

Technology of genetic engineered is an alternative way to improve both the quality and the quanti... more Technology of genetic engineered is an alternative way to improve both the quality and the quantity of agricultural crops. Genetically Engineered Plants (GEPs) as the product of modern technology requires an excellent management strategies especially for the biosafety of the products before being released to commercialized to the public. This study aims determine the policy priorities in making the right decisions in order to GEPs management could be more effective and sustainable while reducing the side effects of this technology to the environment and human health. System analysis approach to take decisions using the Analytical Hierarchy Process (AHP) and Interpretative Structural Modeling (ISM). The outputs of possible policy making based on expert justifications were divided into four (4) levels, i.e.: Focus, Factor, Criteria and Alternative way level. The synthesized of experts justifications on environmental, economic, social and technological factors, give a nearly equal (eigen) values to the previous four levels, so they are concluded in having the same priority in managing of GEPs. The gene flow of GEPs to non GEPs was the most important element to be considered with the eigen values 0.278. The increase of farmer's income with eigen values 0.358 is considered as the most important criteria of economic factor. GEPs safety to human health (0.464) is the preferred social elements. Last but not least, the human resource capability in doing biosafety test (0.580) was the most important criteria for the technology factor. Based on the alternatives compiled by the experts, law enforcement elements of the rules must be done by 0.187 eigen valuescompared with other alternatives. Also based on ISM (Interpretative Structural Modeling) quadrant matrix, alternative elements are scattered into three quadrants; dependence, linkage and independent.

Effect of Transgenic Rice Cry 1A(b) Gene to Nontarget Insects Population in a Limited Field Test.... more Effect of Transgenic Rice Cry 1A(b) Gene to Nontarget Insects Population in a Limited Field Test. Transgenic rice cv. Rojolele (Line 6.11 ±), containing genes cry (IA (b)) of the bacterium Bacillus thuringiensis was advanced to the seventh generations, and tested at a limited field test in Indramayu, West Java, compared to Cisadane, Ciherang, and IR64. A non-transformed of Rojolele cultivar was used as an isogenik line control and was planted as border rows. This study was carried out to meet biosafety requirements of the biological environment, and followed all regulations stipulated in the Guidelines of Biosafety Genetic Engineering Products of Plants Series. Observation was made on the non-target insects and natural enemies in the field. Results indicated that non-target insects, including whiteback plant hopper (WPP) (Sogatella furcifera), brown hopper (Nilaparvata lugens) and Cnaphalocrosis medinalis, were found in the field, with different levels of population. Whiteback plant...

Fakultas Biologi Universitas Nasional., 2016

Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Pro... more Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Province . Bt rice genetically engineered plants (GEPs) having endurance to stem borer insect was developed by the Research Center for Biotechnology LIPI. Compared to conventional plants, the GEP has to obtain a certificate of biosafety and food safety before being released and used by public. It is necessary to study the sustainability of GEP based on indications of sustainable development. The research objective was to determine the sustainability of Bt rice plant based on attributes that construct the dimensions of ecological, economic, social, technological and institutional law. Analysis of the data used Multi Dimensional Scaling (MDS), and the results were expressed in the index form of sustainable management of GEP. Results of the study showed that the sustainability of multidimensional GEP Bt rice corresponds to the criteria of fair with the value 58.99%, except for the technologica...

IOP Conference Series: Earth and Environmental Science, 2020

The development of functional food is one of the government’s programs to improve food security i... more The development of functional food is one of the government’s programs to improve food security in Indonesia. Increasing cases of degenerative diseases such as obesity, diabetes, hypertension, and cancer have triggered people to look for functional food ingredients, especially local resource-based food sources in each region to explore the benefits and nutritional content of health. Arrowroot (Maranta arundinaceae L.) is an alternative crop-source of carbohydrate with enormous potential to be developed. Flour products from arrowroot plant have special features, which are easy to digest because the content of the glycemic index (IG) is low so it is very good for health. This plant is generally propagated by vegetation, so it has a narrow genetic diversity. Various efforts have been made to improve the genetic diversity of arrowroot plants, in order to obtain superior types of arrowroot plants with high productivity. This study aims to understand and explore the primacy of arrowroot p...

elib.pdii.lipi.go.id

Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry... more Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry (crylB-cry\ Aa) dan cry IB dengan promoter terinduksi pelukaan (mpi), teiah didapat. Fusi dua gen cry dan penggunaan promoter terinduksi pelukaan (mpi) ditujukan ...

Jurnal Pengkajian dan Pengembangan Teknologi Pertanian, Jul 1, 2015

ABSTRACT Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East... more ABSTRACT Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Province . Bt rice genetically engineered plants (GEPs) having endurance to stem borer insect was developed by the Research Center for Biotechnology LIPI. Compared to conventional plants, the GEP has to obtain a certificate of biosafety and food safety before being released and used by public. It is necessary to study the sustainability of GEP based on indications of sustainable development. The research objective was to determine the sustainability of Bt rice plant based on attributes that construct the dimensions of ecological, economic, social, technological and institutional law. Analysis of the data used Multi Dimensional Scaling (MDS), and the results were expressed in the index form of sustainable management of GEP. Results of the study showed that the sustainability of multidimensional GEP Bt rice corresponds to the criteria of fair with the value 58.99%, except for the technological dimension with the value of 46.71%, which was classified as less sustainable. In addition, lists of value for other dimensions are: 73.02% for environmental dimension, 69.3% for economic dimension, 51.22% for social dimension, 54.74% for institution and law dimension, and 46.71% for technology dimension that was on less sustainable value. The results also showed ten leverage factors that might affect the increasing the sustainability index of all dimensions. These factors are: environmental dimensions (possibility of crossing genetic materials from GE crops to non-GE crops), economic dimensions (farmers dependency to GE crops, affordable price of GEP seed), social dimensions (informations of GEP, public perception and acception, public participation in decision making), technology dimensions (number of GE plants from internal R&D, capacity building in research and assessment of GEP), institutions and law dimensions (implementation of regulations, GEP labelling). Keywords: Genetically Engineered Plants (GEPs), biosafety, sustainability, Bt Rice ABSTRAK Tanaman padi Bt Produk Rekayasa Genetik (PRG) memiliki sifat ketahanan terhadap hama penggerek batang kuning, hasil pengembangan Puslit Bioteknologi LIPI. Berbeda dengan tanaman konvensional, tanaman PRG harus memperoleh sertifikat keamanan hayati dan keamanan pangan sebelum dilepas dan dimanfaatkan oleh masyarakat. Kajian keberlanjutan PRG berdasarkan indikasi pembangunan berkelanjutan telah dilakukan menggunakan metode expert survey dan pemilihan responden dari kalangan pakar di wilayah Jawa Barat dan Jawa Timur. Tujuan penelitian adalah mengetahui keberlanjutan tanaman Padi Bt PRG berdasarkan atribut-atribut yang menyusun dimensi ekologi, ekonomi, sosial, teknologi serta hukum kelembagaan. Analisis data yang digunakan adalah Multi Dimensional Scaling (MDS) yang hasilnya dinyatakan dalam bentuk indeks keberlanjutan terhadap setiap dimensi yang dikaji. Hasil kajian terhadap multidimensi keberlanjutan tergolong pada kriteria cukup berkelanjutan dengan nilai 58,99%, kecuali untuk dimensi teknologi dengan nilai 46,71% yang tergolong kurang berkelanjutan. Nilai keberlanjutan untuk dimensi lingkungan 73,02%, dimensi ekonomi 69,30%, dimensi sosial 51,22% dan dimensi hukum kelembagaan 54,74%. Diperoleh sepuluh faktor pengungkit ( leverage factor ) yang dapat mempengaruhi peningkatan indeks keberlanjutan yaitu dari dimensi ekologi (Kemungkinan terjadinya perpindahan ( crossing ) material genetik dari tanaman PRG ke tanaman non-PRG), dimensi ekonomi (Ketergantungan petani pada tanaman PRG, Harga beli benih PRG yang terjangkau), dimensi sosial (persepsi dan penerimaan masyarakat, keterlibatan publik dalam pengambilan keputusan), dimensi teknologi (jumlah tanaman PRG hasil litbang sendiri, kemampuan SDM melakukan riset dan pengujian tanaman PRG) serta dimensi hukum kelembagaan (implementasi peraturan dan undang-undang, labeling terhadap PRG). Kata kunci : Produk Rekayasa Genetik (PRG), keamanan hayati, keberlanjutan (sustainability), padi Bt

Jurnal Pengkajian dan Pengembangan Teknologi Pertanian, Jul 1, 2015

Biosaintifika: Journal of Biology & Biology Education

Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitabl... more Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitable for diabetics and other degenerative diseases. It needs to be developed to reduce the dependence on imports or to substitute wheat flour. The objective of the study was to obtain superior arrowroot seedlings and observe the effect of gamma-ray irradiation on the density and position of stomata on arrowroot leaves. In this research, the irradiation of five arrowroot accessions of second-generation (MV1) with gamma rays (0, 10, 20, 30, 40, and 50 Gray) was carried out to obtain mutants with superior character so that they could be used as parent plants for arrowroot flour production. The treatments administered were arrowroot accessions (V), gamma-ray radiation dose (R), and their interaction. The qualitative and quantitative characters on plant characteristics, tuber production, and arrowroot leaves' stomata were observed. The research results showed that increased production is pri...

Jurnal Hama dan Penyakit Tumbuhan Tropika, 2009

The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field... more The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field to non target insects. Transgenic rice plant Rojolele cultivar (lines 6.11(+), 6.11-48, 6.11, 11.21-39, and 11.21-48) that contain cryIA(b) gene from Bacillus thuringiensis was obtained. These lines were expected resistance to stem borer that is considered as one of primary pests. At seven (T6) generation, limited field trial was conducted in Karawang-West Java. Cilosari cultivar (medium resistant control) and IR 62 (susceptible control) were included as standars as well as a non transformed cultivar Rojolele isogenic control and border along with other plants in the surrounding area. The testing was done to meet the biosafety requirements as stipulated in Assessment Guidelines of Living Modified Organism Biosafety Plant Series. Observation was carried out on non targert insect such as other pest insect and general predator in the field. The results showed that non target pest ...

Inflammatory Bowel Diseases, 2006

The plantain banana (cultivar nangka) is among the most valued crop plants in the tropical world.... more The plantain banana (cultivar nangka) is among the most valued crop plants in the tropical world. However, the commercially attainable yields are very low compared to other food crops. Somatic embryogenesis has the great potential for rapid and efficient regeneration of plantlets and offers opportunities for large-scale production of plant material. Genetic modification on embryogenic structure offers the opportunity to add desirable gene characteristic. The objective of this study was to evaluate the different medium combination for the induction and optimization of embryogenic formation from immature male flowers. The explants of inflorescence was sampled one month after bunch emergence and approximately 15-20 cm below the last female flower hand. Response of explants and percentage of inflorescence clusters forming nodular callus were recorded and noted. Result of the experiment showed that embryogenic callus could be induced from immature male flowers. The initiation of callus i...

The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field... more The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field to non target insects. Transgenic rice plant Rojolele cultivar (lines 6.11(+), 6.11-48, 6.11, 11.21-39, and 11.21-48) that contain cryIA(b) gene from Bacillus thuringiensis was obtained. These lines were expected resistance to stem borer that is considered as one of primary pests. At seven (T6) generation, limited field trial was conducted in Karawang-West Java. Cilosari cultivar (medium resistant control) and IR 62 (susceptible control) were included as standars as well as a non transformed cultivar Rojolele isogenic control and border along with other plants in the surrounding area. The testing was done to meet the biosafety requirements as stipulated in Assessment Guidelines of Living Modified Organism Biosafety Plant Series. Observation was carried out on non targert insect such as other pest insect and general predator in the field. The results showed that non target pest insect wh...

Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto, Jul 20, 2020

Improving the quality of rice crops is the first priority to reach the high productivity and food... more Improving the quality of rice crops is the first priority to reach the high productivity and food security in Indonesia. Food needs and nutritional adequacy of the community can be met if the quality of rice increases, so the goal of food self-sufficiency can be realized. The policy of importing rice should immediately end by providing premium-quality rice so that major food needs can be provided. Rice production in Indonesia should be continuously improved to balance with the increasing of growth population every year. Various efforts have been made such as improving the quality of rice to provide better results. The research objectives were selection and evaluation of rice varieties through participatory plant breeding with rice field farmer respondents. Rice farmers from Cibinong, Bogor and surrounding areas were asked to provide an assessment of the morphological characteristics of cross rice crops. The research objectives were selection and evaluation of rice varieties through participatory plant breeding with rice field farmer respondents. Rice farmers from Cibinong, Bogor and surrounding areas were asked to provide an assessment of the morphological characteristics of cross rice crops. The highest assessment result was P3 strain (402) selected by 15 farmers, then P1 (275) selected by 11 farmers. The most preferred varieties of rice are Limboto and IR 64.

Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry... more Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry (crylB-cry\ Aa) dan cry IB dengan promoter terinduksi pelukaan (mpi), teiah didapat. Fusi dua gen cry dan penggunaan promoter terinduksi pelukaan (mpi) ditujukan ...

Al-Kauniyah: Jurnal Biologi

AbstrakPengujian stabilitas genotip merupakan hal yang penting dalam perakitan varietas padi gog... more AbstrakPengujian stabilitas genotip merupakan hal yang penting dalam perakitan varietas padi gogo. Metode pengujian yang sering dilakukan menggunakan analisis ANOVA gabungan yang harus memenuhi normalitas data dan homogenitas ragam. Terkadang data yang diperoleh tidak dapat memenuhi asumsi, sehingga diperlukan analisis alternatif menggunakan metode kualitatif, yaitu Additive Main Effect and Main Interaction (AMMI). Penelitian menggunakan empat belas genotip padi gogo pada tujuh lokasi, dengan rancangan percobaan rancangan acak kelompok. Hasil analisis AMMI1 menunjukkan bahwa didapatkan tujuh genotip dengan nilai rerata produksi di atas rata-rata umum yaitu G29, G31, G33, G34, G35, G37, dan G42 (Inpago 9). Inpago 9 sebagai genotip dengan rata-rata produksi tertinggi di seluruh lokasi. Hasil analisis AMMI2 didapatkan genotip dengan kemampuan adaptasi spesifik lokasi dan beradaptasi pada seluruh lokasi. Dua genotip beradaptasi baik di lokasi Probolinggo yaitu G39 dan G40, sedangkan G2...

GMO Biosafety Research, 2015

Technology of genetic engineered is an alternative way to improve both the quality and the quanti... more Technology of genetic engineered is an alternative way to improve both the quality and the quantity of agricultural crops. Genetically Engineered Plants (GEPs) as the product of modern technology requires an excellent management strategies especially for the biosafety of the products before being released to commercialized to the public. This study aims determine the policy priorities in making the right decisions in order to GEPs management could be more effective and sustainable while reducing the side effects of this technology to the environment and human health. System analysis approach to take decisions using the Analytical Hierarchy Process (AHP) and Interpretative Structural Modeling (ISM). The outputs of possible policy making based on expert justifications were divided into four (4) levels, i.e.: Focus, Factor, Criteria and Alternative way level. The synthesized of experts justifications on environmental, economic, social and technological factors, give a nearly equal (eigen) values to the previous four levels, so they are concluded in having the same priority in managing of GEPs. The gene flow of GEPs to non GEPs was the most important element to be considered with the eigen values 0.278. The increase of farmer's income with eigen values 0.358 is considered as the most important criteria of economic factor. GEPs safety to human health (0.464) is the preferred social elements. Last but not least, the human resource capability in doing biosafety test (0.580) was the most important criteria for the technology factor. Based on the alternatives compiled by the experts, law enforcement elements of the rules must be done by 0.187 eigen valuescompared with other alternatives. Also based on ISM (Interpretative Structural Modeling) quadrant matrix, alternative elements are scattered into three quadrants; dependence, linkage and independent.

Effect of Transgenic Rice Cry 1A(b) Gene to Nontarget Insects Population in a Limited Field Test.... more Effect of Transgenic Rice Cry 1A(b) Gene to Nontarget Insects Population in a Limited Field Test. Transgenic rice cv. Rojolele (Line 6.11 ±), containing genes cry (IA (b)) of the bacterium Bacillus thuringiensis was advanced to the seventh generations, and tested at a limited field test in Indramayu, West Java, compared to Cisadane, Ciherang, and IR64. A non-transformed of Rojolele cultivar was used as an isogenik line control and was planted as border rows. This study was carried out to meet biosafety requirements of the biological environment, and followed all regulations stipulated in the Guidelines of Biosafety Genetic Engineering Products of Plants Series. Observation was made on the non-target insects and natural enemies in the field. Results indicated that non-target insects, including whiteback plant hopper (WPP) (Sogatella furcifera), brown hopper (Nilaparvata lugens) and Cnaphalocrosis medinalis, were found in the field, with different levels of population. Whiteback plant...

Fakultas Biologi Universitas Nasional., 2016

Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Pro... more Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Province . Bt rice genetically engineered plants (GEPs) having endurance to stem borer insect was developed by the Research Center for Biotechnology LIPI. Compared to conventional plants, the GEP has to obtain a certificate of biosafety and food safety before being released and used by public. It is necessary to study the sustainability of GEP based on indications of sustainable development. The research objective was to determine the sustainability of Bt rice plant based on attributes that construct the dimensions of ecological, economic, social, technological and institutional law. Analysis of the data used Multi Dimensional Scaling (MDS), and the results were expressed in the index form of sustainable management of GEP. Results of the study showed that the sustainability of multidimensional GEP Bt rice corresponds to the criteria of fair with the value 58.99%, except for the technologica...

IOP Conference Series: Earth and Environmental Science, 2020

The development of functional food is one of the government’s programs to improve food security i... more The development of functional food is one of the government’s programs to improve food security in Indonesia. Increasing cases of degenerative diseases such as obesity, diabetes, hypertension, and cancer have triggered people to look for functional food ingredients, especially local resource-based food sources in each region to explore the benefits and nutritional content of health. Arrowroot (Maranta arundinaceae L.) is an alternative crop-source of carbohydrate with enormous potential to be developed. Flour products from arrowroot plant have special features, which are easy to digest because the content of the glycemic index (IG) is low so it is very good for health. This plant is generally propagated by vegetation, so it has a narrow genetic diversity. Various efforts have been made to improve the genetic diversity of arrowroot plants, in order to obtain superior types of arrowroot plants with high productivity. This study aims to understand and explore the primacy of arrowroot p...

elib.pdii.lipi.go.id

Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry... more Padi transgenik tahan penggerek batang yang masing-masing mengandung gen crylAb, fusi dna gen cry (crylB-cry\ Aa) dan cry IB dengan promoter terinduksi pelukaan (mpi), teiah didapat. Fusi dua gen cry dan penggunaan promoter terinduksi pelukaan (mpi) ditujukan ...

Jurnal Pengkajian dan Pengembangan Teknologi Pertanian, Jul 1, 2015

ABSTRACT Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East... more ABSTRACT Sustainability Analysis of Genetically Engineered Rice Management i n West Java and East Java Province . Bt rice genetically engineered plants (GEPs) having endurance to stem borer insect was developed by the Research Center for Biotechnology LIPI. Compared to conventional plants, the GEP has to obtain a certificate of biosafety and food safety before being released and used by public. It is necessary to study the sustainability of GEP based on indications of sustainable development. The research objective was to determine the sustainability of Bt rice plant based on attributes that construct the dimensions of ecological, economic, social, technological and institutional law. Analysis of the data used Multi Dimensional Scaling (MDS), and the results were expressed in the index form of sustainable management of GEP. Results of the study showed that the sustainability of multidimensional GEP Bt rice corresponds to the criteria of fair with the value 58.99%, except for the technological dimension with the value of 46.71%, which was classified as less sustainable. In addition, lists of value for other dimensions are: 73.02% for environmental dimension, 69.3% for economic dimension, 51.22% for social dimension, 54.74% for institution and law dimension, and 46.71% for technology dimension that was on less sustainable value. The results also showed ten leverage factors that might affect the increasing the sustainability index of all dimensions. These factors are: environmental dimensions (possibility of crossing genetic materials from GE crops to non-GE crops), economic dimensions (farmers dependency to GE crops, affordable price of GEP seed), social dimensions (informations of GEP, public perception and acception, public participation in decision making), technology dimensions (number of GE plants from internal R&D, capacity building in research and assessment of GEP), institutions and law dimensions (implementation of regulations, GEP labelling). Keywords: Genetically Engineered Plants (GEPs), biosafety, sustainability, Bt Rice ABSTRAK Tanaman padi Bt Produk Rekayasa Genetik (PRG) memiliki sifat ketahanan terhadap hama penggerek batang kuning, hasil pengembangan Puslit Bioteknologi LIPI. Berbeda dengan tanaman konvensional, tanaman PRG harus memperoleh sertifikat keamanan hayati dan keamanan pangan sebelum dilepas dan dimanfaatkan oleh masyarakat. Kajian keberlanjutan PRG berdasarkan indikasi pembangunan berkelanjutan telah dilakukan menggunakan metode expert survey dan pemilihan responden dari kalangan pakar di wilayah Jawa Barat dan Jawa Timur. Tujuan penelitian adalah mengetahui keberlanjutan tanaman Padi Bt PRG berdasarkan atribut-atribut yang menyusun dimensi ekologi, ekonomi, sosial, teknologi serta hukum kelembagaan. Analisis data yang digunakan adalah Multi Dimensional Scaling (MDS) yang hasilnya dinyatakan dalam bentuk indeks keberlanjutan terhadap setiap dimensi yang dikaji. Hasil kajian terhadap multidimensi keberlanjutan tergolong pada kriteria cukup berkelanjutan dengan nilai 58,99%, kecuali untuk dimensi teknologi dengan nilai 46,71% yang tergolong kurang berkelanjutan. Nilai keberlanjutan untuk dimensi lingkungan 73,02%, dimensi ekonomi 69,30%, dimensi sosial 51,22% dan dimensi hukum kelembagaan 54,74%. Diperoleh sepuluh faktor pengungkit ( leverage factor ) yang dapat mempengaruhi peningkatan indeks keberlanjutan yaitu dari dimensi ekologi (Kemungkinan terjadinya perpindahan ( crossing ) material genetik dari tanaman PRG ke tanaman non-PRG), dimensi ekonomi (Ketergantungan petani pada tanaman PRG, Harga beli benih PRG yang terjangkau), dimensi sosial (persepsi dan penerimaan masyarakat, keterlibatan publik dalam pengambilan keputusan), dimensi teknologi (jumlah tanaman PRG hasil litbang sendiri, kemampuan SDM melakukan riset dan pengujian tanaman PRG) serta dimensi hukum kelembagaan (implementasi peraturan dan undang-undang, labeling terhadap PRG). Kata kunci : Produk Rekayasa Genetik (PRG), keamanan hayati, keberlanjutan (sustainability), padi Bt

Jurnal Pengkajian dan Pengembangan Teknologi Pertanian, Jul 1, 2015

Biosaintifika: Journal of Biology & Biology Education

Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitabl... more Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitable for diabetics and other degenerative diseases. It needs to be developed to reduce the dependence on imports or to substitute wheat flour. The objective of the study was to obtain superior arrowroot seedlings and observe the effect of gamma-ray irradiation on the density and position of stomata on arrowroot leaves. In this research, the irradiation of five arrowroot accessions of second-generation (MV1) with gamma rays (0, 10, 20, 30, 40, and 50 Gray) was carried out to obtain mutants with superior character so that they could be used as parent plants for arrowroot flour production. The treatments administered were arrowroot accessions (V), gamma-ray radiation dose (R), and their interaction. The qualitative and quantitative characters on plant characteristics, tuber production, and arrowroot leaves' stomata were observed. The research results showed that increased production is pri...

Jurnal Hama dan Penyakit Tumbuhan Tropika, 2009

The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field... more The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field to non target insects. Transgenic rice plant Rojolele cultivar (lines 6.11(+), 6.11-48, 6.11, 11.21-39, and 11.21-48) that contain cryIA(b) gene from Bacillus thuringiensis was obtained. These lines were expected resistance to stem borer that is considered as one of primary pests. At seven (T6) generation, limited field trial was conducted in Karawang-West Java. Cilosari cultivar (medium resistant control) and IR 62 (susceptible control) were included as standars as well as a non transformed cultivar Rojolele isogenic control and border along with other plants in the surrounding area. The testing was done to meet the biosafety requirements as stipulated in Assessment Guidelines of Living Modified Organism Biosafety Plant Series. Observation was carried out on non targert insect such as other pest insect and general predator in the field. The results showed that non target pest ...

Inflammatory Bowel Diseases, 2006

The plantain banana (cultivar nangka) is among the most valued crop plants in the tropical world.... more The plantain banana (cultivar nangka) is among the most valued crop plants in the tropical world. However, the commercially attainable yields are very low compared to other food crops. Somatic embryogenesis has the great potential for rapid and efficient regeneration of plantlets and offers opportunities for large-scale production of plant material. Genetic modification on embryogenic structure offers the opportunity to add desirable gene characteristic. The objective of this study was to evaluate the different medium combination for the induction and optimization of embryogenic formation from immature male flowers. The explants of inflorescence was sampled one month after bunch emergence and approximately 15-20 cm below the last female flower hand. Response of explants and percentage of inflorescence clusters forming nodular callus were recorded and noted. Result of the experiment showed that embryogenic callus could be induced from immature male flowers. The initiation of callus i...

The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field... more The impact of transgenic rice expressing cryIA(b) gene for stem borer resistance at limited field to non target insects. Transgenic rice plant Rojolele cultivar (lines 6.11(+), 6.11-48, 6.11, 11.21-39, and 11.21-48) that contain cryIA(b) gene from Bacillus thuringiensis was obtained. These lines were expected resistance to stem borer that is considered as one of primary pests. At seven (T6) generation, limited field trial was conducted in Karawang-West Java. Cilosari cultivar (medium resistant control) and IR 62 (susceptible control) were included as standars as well as a non transformed cultivar Rojolele isogenic control and border along with other plants in the surrounding area. The testing was done to meet the biosafety requirements as stipulated in Assessment Guidelines of Living Modified Organism Biosafety Plant Series. Observation was carried out on non targert insect such as other pest insect and general predator in the field. The results showed that non target pest insect wh...