Amel Asely - Benha University (original) (raw)
Papers by Amel Asely
Benha Veterinary Medical Journal
Aquaculture has been a growing provider of animal protein industry in recent decades. Tilapia is ... more Aquaculture has been a growing provider of animal protein industry in recent decades. Tilapia is a very valued fish cultivated across a vast geographical region in numerous countries, including Egypt. Tilapia has the advantage of being tolerant of a wide range of environmental circumstances and utilizing food from the lowest trophic levels. In recent decades, aquaculture has become a greater contribution to animal protein supply. Tilapia is a highly valued fish grown in a wide range of nations, including Egypt, as a source of animal protein. Tilapia could be tolerant to various environmental conditions. Some natural bio-friendly feed additives such as probiotics, prebiotics, and synbiotics are become a new approach in aquaculture industry to maintain aquaculture species health and a good environment at the same time produce chemical and antibiotic-free aquaculture product they become popular dietary supplements with the potential to increase not only growth performance, but also immunological competence and general well-being of fish and crustaceans. The prebiotics preparations are known to be important feed additives to be used in the aquaculture industry to sustain both aquatic species and environmental health. This makes them popular incorporations to enhance not only the growth indicators but also the immunological competence and general well-being of fish and crustaceans. The current review paper focuses on the possible effects of phospholipids (P.L) containing prebiotics on the health, immunological response, growth performance, and survival of oreochromis niloticus.
Egyptian Journal of Veterinary Sciences
I N the current study, clinically diseased cultured Oreochromis niloticus at various life stages ... more I N the current study, clinically diseased cultured Oreochromis niloticus at various life stages were collected from different fish farms within the governorates of Qalyubia, Kafr El-Sheikh, Sharqia, and Port Said. The clinical pictures and gross lesions were recorded. Bacterial pathogen isolation and identification were accomplished using both traditional and molecular techniques. For molecular characterization, traditional PCR was employed to confirm the biochemically identified bacteria using the 16S rRNA. The pathogenicity of the isolates was examined, and histopathological findings were recorded for each. At the farm site examination, the infected fish displayed general septicemic signs such as skin hemorrhages and ulcerations, uni-and bilateral exophthalmia, congested internal organs, and significant mortality. The overall prevalence of bacterial infection was (26.2%). Streptococcus agalactiae was the most prevalent bacteria recovered from clinically diseased juveniles (15.5%), with the summer season exhibiting the highest incidence. The retrieved bacterial isolates were Streptococcus agalactiae (S. agalactiae) (50 isolates, 15.5%), Streptococcus faecalis (S. faecalis) (5 isolates, 1.5%), Enterococcus faecium (E. faecium) (37 isolates, 11.4%), and Lactococcus garviae (L. garviae) (55 isolates, 17%) were isolated from infected juveniles in the autumn (55 isolates, 17%) and adults in the summer (20 isolates, 6.2%). According to the results of this investigation, streptococcal infection, specifically S. agalactiae, S. faecalis, E. faecium, and L. garviae (strain I and II), could be a significant contributor to tilapia mortality during the summer.
Overall evaluation of the replacement of fermented soybean to fish meal in juvenile white shrimp, Litopenaeus vannamei diet: growth, health status, and hepatopancreas histomorphology
This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybea... more This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybean meal (FSBM) for 12 weeks on the growth performance, feed utilization, immunological parameters, antioxidant enzymes assays and lipid peroxidation, digestive enzymes, and histopathological analysis of juvenile Litopeneaus vannamei (L. vannamei). By substituting 0.0%, 20%, 30%, and 40% FSBM for fishmeal (w/w), four isonitrogenous diets were generated. A total of 300 juvenile L. vannamei(1.59 ±0.01 g) were randomly allocated to the experimental fiber tanks at a rate of fifteen shrimp per tank, with three replicates for each treatment. Growth performance and feed utilization decline considerably (P < 0.05) with increasing amounts of FM replacement with FSBM in diets. In comparison to the juveniles fed the other experimental diets, the diet containing a moderate level of FM replacement (20% FSBM) considerably enhanced growth performance and feed consumption during the feeding trial. The ...
Amphora coffeaeformis diatom involved in reducing the susceptibility of Nile tilapia ( Oreochromis niloticus ) fingerlings to Aeromonas hydrophila infection by boosting immune and antioxidant responses and improving growth performance indicators
Aquaculture Research
Effect of dietary administration of Echinacea purpurea on immune responses, histopathological alteration and microbial safety in Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila
Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showe... more Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showed extensive hyphal growth on the skin, fins, gills and eyes. The isolate was molecular identified through 18S rRNA gene and internal transcript spacer, ITS, sequencing. The isolate showed 18S rRNA gene nucleotide identity, 91.6%, and ITS homology with Saprolegnia parasitica. Scanning electron microscopy was applied for evaluating the pathogenicity for the retrieved isolate; which proved its high virulence accompanied with the high mortality rate reaching 100% of the infected fingerlings.
Recuperative effects of honey bee pollen, ginger (Zingiber officinale), and Moringa oleifera in Nile tilapia (Oreochromis niloticus L.) after sub-lethal exposure to dimethoate
Aquaculture, 2021
Abstract This study investigated the efficacies of honey bee pollen (HBP), ginger (Zingiber offic... more Abstract This study investigated the efficacies of honey bee pollen (HBP), ginger (Zingiber officinale), and Moringa oleifera leaves (MOL) as feed additives to counteract dimethoate (DM) toxicity in Nile tilapia (Oreochromis niloticus L.). The fish were divided into eight treatment groups: G1 (control group; basal diet + no DM exposure), G2 (2.5% HBP-supplemented diet + no DM exposure), G3 (2.5% ginger-supplemented diet + no DM exposure), G4 (2.5% MOL-supplemented diet + no DM exposure), G5 (basal diet + DM exposure), G6 (2.5% HBP-supplemented diet + DM exposure), G7 (2.5% ginger-supplemented diet + DM exposure), and G8 (2.5% MOL-supplemented diet + DM exposure). The study was conducted over 30 days, with blood and tissue sample collection on days 15 and 30. Exposure to DM significantly decreased the serum levels of total protein and globulin, while significantly increasing the glucose, total cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, creatinine, creatinine kinase, liver malondialdehyde, total-superoxide dismutase, and catalase levels. By contrast, dietary supplementation of DM-exposed fish with HBP, ginger, or MOL significantly increased the total protein, albumin, and globulin levels and significantly decreased the glucose and total cholesterol levels compared with DM-exposed fish that were fed the basal diet. DM exposure also significantly increased the number of chromosomal aberrations compared with the control, but this effect was ameliorated by addition of the feed additives. The liver, kidneys, and gills had normal histological structures in the control group and the supplemented groups with no DM exposure (G2–G4). DM exposure had damaging effects, with multiple areas of hemorrhage, edema, and necrosis being observed. However, the severity of this was ameliorated by the addition of HBP, ginger, or MOL to the diet. In conclusion, dietary supplementation with HBP, ginger, or MOL improved the antioxidant capacity of Nile tilapia to cope with the biochemical changes, chromosomal aberrations, and pathological alterations that are induced by DM exposure.
Aquaculture Research, 2021
The constantly increasing rate at which scientific papers are published makes it difficult for re... more The constantly increasing rate at which scientific papers are published makes it difficult for researchers to identify papers that currently impact the research field of their interest. Hence, approaches to effectively identify papers of high impact have attracted great attention in the past. In this work, we present a method that seeks to rank papers based on their estimated short-term impact, as measured by the number of citations received in the near future. Similar to previous work, our method models a researcher as she explores the paper citation network. The key aspect is that we incorporate an attentionbased mechanism, akin to a time-restricted version of preferential attachment, to explicitly capture a researcher's preference to read papers which received a lot of attention recently. A detailed experimental evaluation on four real citation datasets across disciplines, shows that our approach is more effective than previous work in ranking papers based on their short-term impact.
Turkish Journal of Fisheries and Aquatic Sciences, 2012
This study aimed at identifying the effects of propolis and honeybee pollen (HBP) on growth perfo... more This study aimed at identifying the effects of propolis and honeybee pollen (HBP) on growth performance, fecundity and some hematological indices of liver and kidney functions of Nile tilapia ''Oreochromis niloticus'' supplemented with 2.5% of propolis or HBP in diet for 21 days. The results showed that dietary propolis or HBP significantly (P<0.05) improved Specific Growth Rate (SGR), Average Daily Gain (ADG) and Feed Efficiency ratio (FER). Propolis significantly (P<0.0001) increased the percentage of O. niloticus with ripened eggs. Microscopically, the ovaries were seen to contain a large number of oocytes >4 mm in the treated groups. In male, HBP feeding significantly (P<0.05) increased testicular weight, gonadosomatic index and improved the semen quality. Nevertheless, propolis treated males showed a significant (P<0.05) increase in head abnormalities among all groups. Sections from the testes of HBP-fed group appeared highly active and showed accumulated sperms in seminiferous tubules. Propolis or HBP significantly (P<0.001) decreased the serum ALT. Concluding that, supplementation of fish diet with either propolis or honeybee pollen is promising a beneficial effect for fisheries due to its potential improving effect on the growth rate and fecundity and preserving some biochemical indices of liver and kidney functions of O. niloticus.
Aquaculture has been increasingly contributing to animal protein production during the last few d... more Aquaculture has been increasingly contributing to animal protein production during the last few decades. Tilapia is known as one of the highly valuable fish cultured in wide geographical areas in several countries as a source of animal protein including Egypt. Tilapia has the merit of tolerance to a wide range of environmental conditions and ability to utilize food from the lowest trophic levels. The intensification of aquaculture production is usually challenged by infections including bacterial and parasitic burdens, which obligates the use of chemicals and antibiotics to control disease outbreaks. The injudicious use of antibiotics inevitably led to expansion of resistance, mutant pathogenic strains and detrimental effects to fish and consumer health. Therefore, it is imperative to find alternative ecofriendly sources as prebiotics and probiotics which can improve fish health, performance, and immunity without any side effects to the fish themselves or the consumer health. Prebio...
mmunostimulants are a category of the natural substances, which proved effective in controlling t... more mmunostimulants are a category of the natural substances, which proved effective in controlling the disease in wide range of terrestrial animals and in the field of aquaculture. Plant mixed feed named MACH is a preparation made from four plants; namely, pumpkin (Cucurbita moschata) seeds, plantain (Plantago asiatica) seeds, honeysuckle (Lonicera japonica) flowers, and safflower (Carthamus tinctorius) flowers was added to shrimp feed at concentration of 0.2%; shrimp were fed on this mixture for seven days. Immune-related gene expressions were measured on zero, one, three and seven days after start feeding. Prophenol oxidase, antilipopolysaccharide factor and lysozyme genes were studied. The results showed that the antilipopolysacchride factor gene (ALF) was highly expressed on the 3 rd day from feeding and returned to its normal levels on the 7 th day. Lysozyme gene expression reached its peak on the 3 rd day after feeding and returned to its normal values on the 7 th day. The Prophe...
Journal of Advanced Research, 2014
level in females comparing to control. In conclusion, BP can be used as a feed additive for fish ... more level in females comparing to control. In conclusion, BP can be used as a feed additive for fish prone to be raised in integrated fish farms or cage culture due to its potency to chemo-protect against genotoxicity and sperm-teratogenicity persuaded by malathion-exposure.
Saccharomyces cerevisiae increases the acceptability of Nile tilapia (Oreochromis niloticus) to date palm seed meal
Aquaculture Reports
The current study aimed to control the bacterial infection associated with summer mortalities in ... more The current study aimed to control the bacterial infection associated with summer mortalities in Nile tilapia. For determination to the sensitivity of the isolated bacteria to different antimicrobials; Aeromonas Veronii (A. veronii) strian (HY1, HY2, HY3, HY4 and HY6), Citrobacter freundii (C. freundii), Proteus vulgaris (P. vulgaris) and pseudomonas flurosence (P. flurosence) isolates were tested for antimicrobial sensitivity and antimicrobial resistant genes. In order to verify the result of antibiotic sensitivity test; experimental trial was performed to determine the effect of Florofenicol and Erythromycin against infection of O. niloticus with the different pathogenic bacteria (4 strains of A. veroni (A(HY2), A(HY3), A(HY4) and A(HY6)), C. freundi, P. flourosence and P. vulgaris). The results revealed that all tested bacterial isolates were 100 % sensitive to florfenicol and erythromycin, 50% sensitive to gentamycin, 25 % sensitive to nalidixic acid and sulphamethoxazole-trimet...
Corrigendum to “The modulatory effect of mannanoligosaccharide on oxidative status, selected immune parameters and tolerance against low salinity stress in red sea bream (Pagrus major)” [Aquacult. Rep. 16 March (2020) 100278]
Aquaculture Reports, Apr 1, 2020
Benha Veterinary Medical Journal
The current investigation was undertaken to determine the potential causes of summer mass mortali... more The current investigation was undertaken to determine the potential causes of summer mass mortalities among farmed Nile tilapia (Oreochromis niloticus). Pure bacterial colonies were isolated from moribund O. niloticus from 13 different fish farms suffered from high mortalities ranged from (50-80%), during the period from April to October 2018. Fish showed external hemorrhagic spots, skin darkening, abdominal distension and exophthalmia. Internally, congestion and enlargement of internal organs with serous or hemorrhagic fluid was the most obvious picture. The phenotypic and biochemical characterization using API20E identified the bacterial isolates as (A. veronii, A. hydrophila, A. caviae and A. sobria; Aeromonads), (Ps. Fluorescence; Pseudomonas spp), (E. sakazakii and E. cloacae; Enterobacter spp), (C. freundii; Citrobacter spp), (S. odorifera, S. liquefaciens, and S. marcescens; Serratia spp), (S. lutiensis, S. equine; Streptococcus spp), Lactococcus lactis and Proteus vulgaris, with the most prevalence to aeromonads. Most isolates were accurately identified by PCR and gene sequencing. Water physicochemical parameters were measured at the farm sites showed an increase in the pH and ammonia levels. In order to confirm the pathogenicity of the bacterial isolates, an experimental infection was conducted using different doses. The results revealed that A. veronii (HY2) at dose of 9×10 8 cells/ml was the most pathogenic with mortality rate 100 %. This study concludes that A. veronii, C. freundii, P. vulgaris and P. fluorescens are implicated in Nile tilapia summer mortalities, without neglecting the role of water quality in worsening the problem. Bacterial infection.
The efficiency of dietary Sargassum aquifolium on the performance, innate immune responses, antioxidant activity, and intestinal microbiota of Nile Tilapia (Oreochromis niloticus) raised at high stocking density
Journal of Applied Phycology
Pathogens
Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease co... more Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases are considered as alternative approaches for sustainable aquaculture. Essential oils (EOs) are the secondary metabolites of medicinal plants that possess bioactive compounds like terpens, terpenoids, phenylpropenes, and isothiocyanates with synergistic relationship among these compounds. The hydrophobic compounds of EOs can penetrate the bacterial and parasitic cells and cause cell deformities and organelles dysfunctions. Dietary supplementation of EOs also modulate growth, immunity, and infectious disease resistance in aquatic organisms. Published research reports also demonstrated EOs effectiveness against Ichthyophthirius multifiliis, ...
Miswak (Salvadora persica) modulated the growth performance, antioxidative response, and histopathological damage induced by zinc toxicity in Nile tilapia (Oreochromis niloticus)
Environmental Science and Pollution Research
Replacement of dietary fish oil with plant oils improves the immunological responses and the antioxidant status in Oreochromis niloticus exposed to suboptimal temperature
Fish Physiology and Biochemistry
Benha Veterinary Medical Journal
Aquaculture has been a growing provider of animal protein industry in recent decades. Tilapia is ... more Aquaculture has been a growing provider of animal protein industry in recent decades. Tilapia is a very valued fish cultivated across a vast geographical region in numerous countries, including Egypt. Tilapia has the advantage of being tolerant of a wide range of environmental circumstances and utilizing food from the lowest trophic levels. In recent decades, aquaculture has become a greater contribution to animal protein supply. Tilapia is a highly valued fish grown in a wide range of nations, including Egypt, as a source of animal protein. Tilapia could be tolerant to various environmental conditions. Some natural bio-friendly feed additives such as probiotics, prebiotics, and synbiotics are become a new approach in aquaculture industry to maintain aquaculture species health and a good environment at the same time produce chemical and antibiotic-free aquaculture product they become popular dietary supplements with the potential to increase not only growth performance, but also immunological competence and general well-being of fish and crustaceans. The prebiotics preparations are known to be important feed additives to be used in the aquaculture industry to sustain both aquatic species and environmental health. This makes them popular incorporations to enhance not only the growth indicators but also the immunological competence and general well-being of fish and crustaceans. The current review paper focuses on the possible effects of phospholipids (P.L) containing prebiotics on the health, immunological response, growth performance, and survival of oreochromis niloticus.
Egyptian Journal of Veterinary Sciences
I N the current study, clinically diseased cultured Oreochromis niloticus at various life stages ... more I N the current study, clinically diseased cultured Oreochromis niloticus at various life stages were collected from different fish farms within the governorates of Qalyubia, Kafr El-Sheikh, Sharqia, and Port Said. The clinical pictures and gross lesions were recorded. Bacterial pathogen isolation and identification were accomplished using both traditional and molecular techniques. For molecular characterization, traditional PCR was employed to confirm the biochemically identified bacteria using the 16S rRNA. The pathogenicity of the isolates was examined, and histopathological findings were recorded for each. At the farm site examination, the infected fish displayed general septicemic signs such as skin hemorrhages and ulcerations, uni-and bilateral exophthalmia, congested internal organs, and significant mortality. The overall prevalence of bacterial infection was (26.2%). Streptococcus agalactiae was the most prevalent bacteria recovered from clinically diseased juveniles (15.5%), with the summer season exhibiting the highest incidence. The retrieved bacterial isolates were Streptococcus agalactiae (S. agalactiae) (50 isolates, 15.5%), Streptococcus faecalis (S. faecalis) (5 isolates, 1.5%), Enterococcus faecium (E. faecium) (37 isolates, 11.4%), and Lactococcus garviae (L. garviae) (55 isolates, 17%) were isolated from infected juveniles in the autumn (55 isolates, 17%) and adults in the summer (20 isolates, 6.2%). According to the results of this investigation, streptococcal infection, specifically S. agalactiae, S. faecalis, E. faecium, and L. garviae (strain I and II), could be a significant contributor to tilapia mortality during the summer.
Overall evaluation of the replacement of fermented soybean to fish meal in juvenile white shrimp, Litopenaeus vannamei diet: growth, health status, and hepatopancreas histomorphology
This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybea... more This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybean meal (FSBM) for 12 weeks on the growth performance, feed utilization, immunological parameters, antioxidant enzymes assays and lipid peroxidation, digestive enzymes, and histopathological analysis of juvenile Litopeneaus vannamei (L. vannamei). By substituting 0.0%, 20%, 30%, and 40% FSBM for fishmeal (w/w), four isonitrogenous diets were generated. A total of 300 juvenile L. vannamei(1.59 ±0.01 g) were randomly allocated to the experimental fiber tanks at a rate of fifteen shrimp per tank, with three replicates for each treatment. Growth performance and feed utilization decline considerably (P < 0.05) with increasing amounts of FM replacement with FSBM in diets. In comparison to the juveniles fed the other experimental diets, the diet containing a moderate level of FM replacement (20% FSBM) considerably enhanced growth performance and feed consumption during the feeding trial. The ...
Amphora coffeaeformis diatom involved in reducing the susceptibility of Nile tilapia ( Oreochromis niloticus ) fingerlings to Aeromonas hydrophila infection by boosting immune and antioxidant responses and improving growth performance indicators
Aquaculture Research
Effect of dietary administration of Echinacea purpurea on immune responses, histopathological alteration and microbial safety in Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila
Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showe... more Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showed extensive hyphal growth on the skin, fins, gills and eyes. The isolate was molecular identified through 18S rRNA gene and internal transcript spacer, ITS, sequencing. The isolate showed 18S rRNA gene nucleotide identity, 91.6%, and ITS homology with Saprolegnia parasitica. Scanning electron microscopy was applied for evaluating the pathogenicity for the retrieved isolate; which proved its high virulence accompanied with the high mortality rate reaching 100% of the infected fingerlings.
Recuperative effects of honey bee pollen, ginger (Zingiber officinale), and Moringa oleifera in Nile tilapia (Oreochromis niloticus L.) after sub-lethal exposure to dimethoate
Aquaculture, 2021
Abstract This study investigated the efficacies of honey bee pollen (HBP), ginger (Zingiber offic... more Abstract This study investigated the efficacies of honey bee pollen (HBP), ginger (Zingiber officinale), and Moringa oleifera leaves (MOL) as feed additives to counteract dimethoate (DM) toxicity in Nile tilapia (Oreochromis niloticus L.). The fish were divided into eight treatment groups: G1 (control group; basal diet + no DM exposure), G2 (2.5% HBP-supplemented diet + no DM exposure), G3 (2.5% ginger-supplemented diet + no DM exposure), G4 (2.5% MOL-supplemented diet + no DM exposure), G5 (basal diet + DM exposure), G6 (2.5% HBP-supplemented diet + DM exposure), G7 (2.5% ginger-supplemented diet + DM exposure), and G8 (2.5% MOL-supplemented diet + DM exposure). The study was conducted over 30 days, with blood and tissue sample collection on days 15 and 30. Exposure to DM significantly decreased the serum levels of total protein and globulin, while significantly increasing the glucose, total cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, creatinine, creatinine kinase, liver malondialdehyde, total-superoxide dismutase, and catalase levels. By contrast, dietary supplementation of DM-exposed fish with HBP, ginger, or MOL significantly increased the total protein, albumin, and globulin levels and significantly decreased the glucose and total cholesterol levels compared with DM-exposed fish that were fed the basal diet. DM exposure also significantly increased the number of chromosomal aberrations compared with the control, but this effect was ameliorated by addition of the feed additives. The liver, kidneys, and gills had normal histological structures in the control group and the supplemented groups with no DM exposure (G2–G4). DM exposure had damaging effects, with multiple areas of hemorrhage, edema, and necrosis being observed. However, the severity of this was ameliorated by the addition of HBP, ginger, or MOL to the diet. In conclusion, dietary supplementation with HBP, ginger, or MOL improved the antioxidant capacity of Nile tilapia to cope with the biochemical changes, chromosomal aberrations, and pathological alterations that are induced by DM exposure.
Aquaculture Research, 2021
The constantly increasing rate at which scientific papers are published makes it difficult for re... more The constantly increasing rate at which scientific papers are published makes it difficult for researchers to identify papers that currently impact the research field of their interest. Hence, approaches to effectively identify papers of high impact have attracted great attention in the past. In this work, we present a method that seeks to rank papers based on their estimated short-term impact, as measured by the number of citations received in the near future. Similar to previous work, our method models a researcher as she explores the paper citation network. The key aspect is that we incorporate an attentionbased mechanism, akin to a time-restricted version of preferential attachment, to explicitly capture a researcher's preference to read papers which received a lot of attention recently. A detailed experimental evaluation on four real citation datasets across disciplines, shows that our approach is more effective than previous work in ranking papers based on their short-term impact.
Turkish Journal of Fisheries and Aquatic Sciences, 2012
This study aimed at identifying the effects of propolis and honeybee pollen (HBP) on growth perfo... more This study aimed at identifying the effects of propolis and honeybee pollen (HBP) on growth performance, fecundity and some hematological indices of liver and kidney functions of Nile tilapia ''Oreochromis niloticus'' supplemented with 2.5% of propolis or HBP in diet for 21 days. The results showed that dietary propolis or HBP significantly (P<0.05) improved Specific Growth Rate (SGR), Average Daily Gain (ADG) and Feed Efficiency ratio (FER). Propolis significantly (P<0.0001) increased the percentage of O. niloticus with ripened eggs. Microscopically, the ovaries were seen to contain a large number of oocytes >4 mm in the treated groups. In male, HBP feeding significantly (P<0.05) increased testicular weight, gonadosomatic index and improved the semen quality. Nevertheless, propolis treated males showed a significant (P<0.05) increase in head abnormalities among all groups. Sections from the testes of HBP-fed group appeared highly active and showed accumulated sperms in seminiferous tubules. Propolis or HBP significantly (P<0.001) decreased the serum ALT. Concluding that, supplementation of fish diet with either propolis or honeybee pollen is promising a beneficial effect for fisheries due to its potential improving effect on the growth rate and fecundity and preserving some biochemical indices of liver and kidney functions of O. niloticus.
Aquaculture has been increasingly contributing to animal protein production during the last few d... more Aquaculture has been increasingly contributing to animal protein production during the last few decades. Tilapia is known as one of the highly valuable fish cultured in wide geographical areas in several countries as a source of animal protein including Egypt. Tilapia has the merit of tolerance to a wide range of environmental conditions and ability to utilize food from the lowest trophic levels. The intensification of aquaculture production is usually challenged by infections including bacterial and parasitic burdens, which obligates the use of chemicals and antibiotics to control disease outbreaks. The injudicious use of antibiotics inevitably led to expansion of resistance, mutant pathogenic strains and detrimental effects to fish and consumer health. Therefore, it is imperative to find alternative ecofriendly sources as prebiotics and probiotics which can improve fish health, performance, and immunity without any side effects to the fish themselves or the consumer health. Prebio...
mmunostimulants are a category of the natural substances, which proved effective in controlling t... more mmunostimulants are a category of the natural substances, which proved effective in controlling the disease in wide range of terrestrial animals and in the field of aquaculture. Plant mixed feed named MACH is a preparation made from four plants; namely, pumpkin (Cucurbita moschata) seeds, plantain (Plantago asiatica) seeds, honeysuckle (Lonicera japonica) flowers, and safflower (Carthamus tinctorius) flowers was added to shrimp feed at concentration of 0.2%; shrimp were fed on this mixture for seven days. Immune-related gene expressions were measured on zero, one, three and seven days after start feeding. Prophenol oxidase, antilipopolysaccharide factor and lysozyme genes were studied. The results showed that the antilipopolysacchride factor gene (ALF) was highly expressed on the 3 rd day from feeding and returned to its normal levels on the 7 th day. Lysozyme gene expression reached its peak on the 3 rd day after feeding and returned to its normal values on the 7 th day. The Prophe...
Journal of Advanced Research, 2014
level in females comparing to control. In conclusion, BP can be used as a feed additive for fish ... more level in females comparing to control. In conclusion, BP can be used as a feed additive for fish prone to be raised in integrated fish farms or cage culture due to its potency to chemo-protect against genotoxicity and sperm-teratogenicity persuaded by malathion-exposure.
Saccharomyces cerevisiae increases the acceptability of Nile tilapia (Oreochromis niloticus) to date palm seed meal
Aquaculture Reports
The current study aimed to control the bacterial infection associated with summer mortalities in ... more The current study aimed to control the bacterial infection associated with summer mortalities in Nile tilapia. For determination to the sensitivity of the isolated bacteria to different antimicrobials; Aeromonas Veronii (A. veronii) strian (HY1, HY2, HY3, HY4 and HY6), Citrobacter freundii (C. freundii), Proteus vulgaris (P. vulgaris) and pseudomonas flurosence (P. flurosence) isolates were tested for antimicrobial sensitivity and antimicrobial resistant genes. In order to verify the result of antibiotic sensitivity test; experimental trial was performed to determine the effect of Florofenicol and Erythromycin against infection of O. niloticus with the different pathogenic bacteria (4 strains of A. veroni (A(HY2), A(HY3), A(HY4) and A(HY6)), C. freundi, P. flourosence and P. vulgaris). The results revealed that all tested bacterial isolates were 100 % sensitive to florfenicol and erythromycin, 50% sensitive to gentamycin, 25 % sensitive to nalidixic acid and sulphamethoxazole-trimet...
Corrigendum to “The modulatory effect of mannanoligosaccharide on oxidative status, selected immune parameters and tolerance against low salinity stress in red sea bream (Pagrus major)” [Aquacult. Rep. 16 March (2020) 100278]
Aquaculture Reports, Apr 1, 2020
Benha Veterinary Medical Journal
The current investigation was undertaken to determine the potential causes of summer mass mortali... more The current investigation was undertaken to determine the potential causes of summer mass mortalities among farmed Nile tilapia (Oreochromis niloticus). Pure bacterial colonies were isolated from moribund O. niloticus from 13 different fish farms suffered from high mortalities ranged from (50-80%), during the period from April to October 2018. Fish showed external hemorrhagic spots, skin darkening, abdominal distension and exophthalmia. Internally, congestion and enlargement of internal organs with serous or hemorrhagic fluid was the most obvious picture. The phenotypic and biochemical characterization using API20E identified the bacterial isolates as (A. veronii, A. hydrophila, A. caviae and A. sobria; Aeromonads), (Ps. Fluorescence; Pseudomonas spp), (E. sakazakii and E. cloacae; Enterobacter spp), (C. freundii; Citrobacter spp), (S. odorifera, S. liquefaciens, and S. marcescens; Serratia spp), (S. lutiensis, S. equine; Streptococcus spp), Lactococcus lactis and Proteus vulgaris, with the most prevalence to aeromonads. Most isolates were accurately identified by PCR and gene sequencing. Water physicochemical parameters were measured at the farm sites showed an increase in the pH and ammonia levels. In order to confirm the pathogenicity of the bacterial isolates, an experimental infection was conducted using different doses. The results revealed that A. veronii (HY2) at dose of 9×10 8 cells/ml was the most pathogenic with mortality rate 100 %. This study concludes that A. veronii, C. freundii, P. vulgaris and P. fluorescens are implicated in Nile tilapia summer mortalities, without neglecting the role of water quality in worsening the problem. Bacterial infection.
The efficiency of dietary Sargassum aquifolium on the performance, innate immune responses, antioxidant activity, and intestinal microbiota of Nile Tilapia (Oreochromis niloticus) raised at high stocking density
Journal of Applied Phycology
Pathogens
Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease co... more Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases are considered as alternative approaches for sustainable aquaculture. Essential oils (EOs) are the secondary metabolites of medicinal plants that possess bioactive compounds like terpens, terpenoids, phenylpropenes, and isothiocyanates with synergistic relationship among these compounds. The hydrophobic compounds of EOs can penetrate the bacterial and parasitic cells and cause cell deformities and organelles dysfunctions. Dietary supplementation of EOs also modulate growth, immunity, and infectious disease resistance in aquatic organisms. Published research reports also demonstrated EOs effectiveness against Ichthyophthirius multifiliis, ...
Miswak (Salvadora persica) modulated the growth performance, antioxidative response, and histopathological damage induced by zinc toxicity in Nile tilapia (Oreochromis niloticus)
Environmental Science and Pollution Research
Replacement of dietary fish oil with plant oils improves the immunological responses and the antioxidant status in Oreochromis niloticus exposed to suboptimal temperature
Fish Physiology and Biochemistry